Skip to main content
×
Home

A new electronic assay enables ultrasensitive detection of diverse biological analytes—nucleic acids, proteins and small molecules—on a single integrated circuit

  • Leyla Soleymani (a1)
Abstract
Abstract

Development of universal biosensors based on electrical readout is currently limited by the difficulty of electrical signal transduction upon capture of neutral analytes. Kelley and co-workers demonstrate an elegant approach wherein an amplified electrical current flows to a multiplexed electrode array in proportion with the binding of nucleic acids, proteins, and small molecules—regardless of their inherent charge. Here we present a commentary on the strengths and limitations of this method.

Copyright
Corresponding author
Address all correspondence to Leyla Soleymani at soleyml@mcmaster.ca
References
Hide All
1. Soleymani L., Fang Z., Sargent E., and Kelley S.O.: Programming the detection limits of biosensors through controlled nanostructuring. Nat. Nanotechnol. 4, 844 (2009).
2. Ziegler A., Koch A., Krockenberger K., and Großhennig A.: Personalized medicine using DNA biomarkers: a review. Hum. Genet. 131, 1627 (2012).
3. Rifai N., Gillette M.A., and Carr S.A.: Protein biomarker discovery and validation: the long and uncertain path to clinical utility. Nat. Biotechnol. 24, 971 (2006).
4. Iliuk A.B., Hu L., and Tao W.A.: Aptamer in bioanalytical applications. Anal. Chem. 83, 4440 (2011).
5. Das J., Cederquist K.B., Zaragoza A.A., Lee P.E., Sargent E.H., and Kelley S.O.: An ultrasensitive universal detector based on neutralizer displacement. Nat. Chem. 4, 642 (2012).
6. Lapierre-Devlin M., Asher C.L., Taft B.J., Gasparac R., Roberts M.A., and Kelley S.O.: Amplified electrocatalysis at DNA-modified nanowires. Nano Lett. 5, 1051 (2005).
7. Drummond T.G., Hill M.G., and Barton J.K.: Electrochemical DNA sensors. Nat. Biotechnol. 21, 1192 (2003).
8. Zeglis B.M. and Barton J.K.: DNA base mismatch detection with bulky rhodium intercalators: synthesis and applications. Nat. Biotechnol. 2, 357 (2007).
9. Wang H., Bhunia A.K., and Lu C.: A microfluidic flow-through device for high throughput electrical lysis of bacterial cells based on continuous dc voltage. Biosens. Bioelectron. 22, 582 (2006).
10. Plaxco K.W. and Soh H.T.: Witch-based biosensors: a new approach towards real-time, in vivo molecular detection. Trends Biotechnol. 29, 1 (2011).
11. Lapierre M.A., O'Keefe M., Taft B.J., and Kelley S.O.: Electrocatalytic detection of pathogenic DNA sequences and antibiotic resistance markers. Anal. Chem. 75, 6327 (2003).
12. Soleymani L., Fang Z., Sun X., Yang H., Taft B., Sargent E., and Kelley S.O.: Nanostructuring of patterned microelectrodes to enhance the sensitivity of electrochemical nucleic acids detection. Angew. Chem. Int. Ed. (International ed. in English). 48, 8457 (2009).
13. Gasparac R., Taft B.J., Lapierre-Devlin M., Lazareck A.D., Xu J.M., and Kelley S.O.: Ultrasensitive electrocatalytic DNA detection with 3D nanoelectrodes. J. Am. Chem. Soc. 126, 12270 (2004).
14. Soleymani L., Fang Z., Kelley S.O., and Sargent E.H.: Integrated nanostructures for direct detection of DNA at attomolar concentrations. Appl. Phys. Lett. 95, 143701 (2009).
15. Yao W., Wang L., Wang H., Zhang X., and Li L.: An aptamer-based electrochemiluminescent biosensor for ATP detection. Biosens. Bioelectron. 24, 3269 (2009).
16. Zuo X., Song S., Zhang J., Pan D., Wang L., and Fan C.: A target-responsive electrochemical aptamer switch (TREAS) for reagentless detection of nanomolar ATP. J. Am. Chem. Soc. 129, 1042 (2007).
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

MRS Communications
  • ISSN: 2159-6859
  • EISSN: 2159-6867
  • URL: /core/journals/mrs-communications
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Metrics

Full text views

Total number of HTML views: 3
Total number of PDF views: 12 *
Loading metrics...

Abstract views

Total abstract views: 123 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 11th December 2017. This data will be updated every 24 hours.