Skip to main content
×
Home
    • Aa
    • Aa

A new electronic assay enables ultrasensitive detection of diverse biological analytes—nucleic acids, proteins and small molecules—on a single integrated circuit

  • Leyla Soleymani (a1)
Abstract
Abstract

Development of universal biosensors based on electrical readout is currently limited by the difficulty of electrical signal transduction upon capture of neutral analytes. Kelley and co-workers demonstrate an elegant approach wherein an amplified electrical current flows to a multiplexed electrode array in proportion with the binding of nucleic acids, proteins, and small molecules—regardless of their inherent charge. Here we present a commentary on the strengths and limitations of this method.

Copyright
Corresponding author
Address all correspondence to Leyla Soleymani at soleyml@mcmaster.ca
References
Hide All
1. L. Soleymani , Z. Fang , E. Sargent , and S.O. Kelley : Programming the detection limits of biosensors through controlled nanostructuring. Nat. Nanotechnol. 4, 844 (2009).

2. A. Ziegler , A. Koch , K. Krockenberger , and A. Großhennig : Personalized medicine using DNA biomarkers: a review. Hum. Genet. 131, 1627 (2012).

3. N. Rifai , M.A. Gillette , and S.A. Carr : Protein biomarker discovery and validation: the long and uncertain path to clinical utility. Nat. Biotechnol. 24, 971 (2006).

4. A.B. Iliuk , L. Hu , and W.A. Tao : Aptamer in bioanalytical applications. Anal. Chem. 83, 4440 (2011).

5. J. Das , K.B. Cederquist , A.A. Zaragoza , P.E. Lee , E.H. Sargent , and S.O. Kelley : An ultrasensitive universal detector based on neutralizer displacement. Nat. Chem. 4, 642 (2012).

6. M. Lapierre-Devlin , C.L. Asher , B.J. Taft , R. Gasparac , M.A. Roberts , and S.O. Kelley : Amplified electrocatalysis at DNA-modified nanowires. Nano Lett. 5, 1051 (2005).

7. T.G. Drummond , M.G. Hill , and J.K. Barton : Electrochemical DNA sensors. Nat. Biotechnol. 21, 1192 (2003).

9. H. Wang , A.K. Bhunia , and C. Lu : A microfluidic flow-through device for high throughput electrical lysis of bacterial cells based on continuous dc voltage. Biosens. Bioelectron. 22, 582 (2006).

10. K.W. Plaxco and H.T. Soh : Witch-based biosensors: a new approach towards real-time, in vivo molecular detection. Trends Biotechnol. 29, 1 (2011).

11. M.A. Lapierre , M. O'Keefe , B.J. Taft , and S.O. Kelley : Electrocatalytic detection of pathogenic DNA sequences and antibiotic resistance markers. Anal. Chem. 75, 6327 (2003).

12. L. Soleymani , Z. Fang , X. Sun , H. Yang , B. Taft , E. Sargent , and S.O. Kelley : Nanostructuring of patterned microelectrodes to enhance the sensitivity of electrochemical nucleic acids detection. Angew. Chem. Int. Ed. (International ed. in English). 48, 8457 (2009).

13. R. Gasparac , B.J. Taft , M. Lapierre-Devlin , A.D. Lazareck , J.M. Xu , and S.O. Kelley : Ultrasensitive electrocatalytic DNA detection with 3D nanoelectrodes. J. Am. Chem. Soc. 126, 12270 (2004).

14. L. Soleymani , Z. Fang , S.O. Kelley , and E.H. Sargent : Integrated nanostructures for direct detection of DNA at attomolar concentrations. Appl. Phys. Lett. 95, 143701 (2009).

15. W. Yao , L. Wang , H. Wang , X. Zhang , and L. Li : An aptamer-based electrochemiluminescent biosensor for ATP detection. Biosens. Bioelectron. 24, 3269 (2009).

16. X. Zuo , S. Song , J. Zhang , D. Pan , L. Wang , and C. Fan : A target-responsive electrochemical aptamer switch (TREAS) for reagentless detection of nanomolar ATP. J. Am. Chem. Soc. 129, 1042 (2007).

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

MRS Communications
  • ISSN: 2159-6859
  • EISSN: 2159-6867
  • URL: /core/journals/mrs-communications
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Metrics

Full text views

Total number of HTML views: 3
Total number of PDF views: 11 *
Loading metrics...

Abstract views

Total abstract views: 114 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 18th October 2017. This data will be updated every 24 hours.