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Amino acid catabolism in the nematodes Heligmosomoides polygyrus and Panagrellus redivivus. 1. Removal of the amino group

  • Barbara D. Grantham (a1) and J. Barrett (a1)


The major transaminase in Heligmosomoides polygyrus, Panagrellus redivivus and rat liver was the 2-oxoglutarate-glutamate system, with relatively few amino acids acting as donors for the pyruvate-alanine and oxaloacetate–aspartate systems. The relative effectiveness of the different amino acid donors in the three transaminase systems was similar in all three tissues. Both H. polygyrus and P. redivivus can oxidatively deaminate a range of L-amino acids, although D-amino acid oxidase activity was low. Serine and threonine dehydratase activity and histidase activity were present in H. polygyrus and P. redivivus and both nematodes were also able to deaminate glutamine, asparagine and arginine. When NAD(H) was the cofactor the glutamate dehydrogenases of H. polygyrus and P. redivivus showed similar regulatory properties to the mammalian enzyme. However, with NADP(H) the results were anomalous. The capacity of both nematodes to transaminate and oxidatively deaminate amino acids was broadly similar and comparable to mammalian tissue. Glutamate dehydrogenase is probably the major route for deamination in these nematodes. A complete sequence of urea cycle enzymes could not be demonstrated in either P. redivivus or H. polygyrus.



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Barrett, J. (1981). Biochemistry of Parasitic Helminths. London: Macmillan.
Barrett, J. & Butterworth, P. E. (1984). Acetaldehyde formation by mitochondria from the free-living nematode Panagrellus redivivus. The Biochemical Journal 221, 535–40.
Bergmeyer, H. U., Gawehn, K. & Grassl, M. (1974). Glutaminase. In Methods of Enzymatic Analysis (ed. H., Bergmeyer), vol. 1, p. 465. Academic Press: New York.
Burren, C. H. (1980). A method for obtaining large numbers of clean infective larvae of Nemato spiroides dubius. Zeitschrift für Parasitenkunde 62, 111–12.
Burns, R. O. (1971). l-Threonine deaminase-biosynthetic (Salmonella typhimurium). In Methods in Enzymology, vol. 17B, (ed. H., Tabor and Tabor, C. W.) pp. 555–60. New York: Academic Press.
Ceriotti, G. (1974). Ornithine carbamoyltransferase. In Methods of Enzymatic Analysis (ed. Bergmeyer, H. U.), vol. 2, pp. 691–8. New York, Academic Press.
Dobson, C. (1961). Certain aspects of the host-parasite relationship of Nematospiroides dubius (Baylis). 1. Resistance of male and female mice to experimental infections. Parasitology 51, 173–9.
Fahien, L. A. & Cohen, P. P. (1964). A kinetic study of carbamoyl phosphate synthetase. Journal of Biological Chemistry 239, 1925–34.
Horowitz, R. J. (1978). Temporal variability patterns and the distributional patterns of stream fishes. Ecological Monographs 48, 307–21.
Magasanik, B., Kaminskas, E. & Kimhi, Y. (1971). Histidase (histidine-ammonia-lyase) (Bacilis subtilis). In Methods in Enzymology (ed. H., Tabor and Tabor, C. W.), vol. 17 B, pp. 4750. New York: Academic Press.
Moroney, M. J. (1962). Facts from Figures. London: Penguin.
Mustafa, T., Komuniecki, R. & Mettrick, D. F. (1978). Cystolic glutamate dehydrogenase in adult Hymenolepis diminuta (Cestoda). Comparative Biochemistry and Physiology 61B, 219–22.
Newsholme, E. A. & Start, C. (1976). Regulation in Metabolism. New York: Wiley.
Rainbow, V. M. T. (1972). Studies on the helminth parasites of small mammals with particular reference to the ecology and physiology of Nematospiroides dubius. Ph.D. thesis, University of London.
Ratner, S. (1970 a). Argininosuccinate synthetase (steer liver). In Methods in Enzymology (ed. H., Tabor and Tabor, C. W.), vol. 17A, pp. 298303. New York: Academic Press.
Ratner, S. (1970 b). Arginino-succinase (steer liver). In Methods in Enzymology (ed. H., Tabor and Tabor, C. W.), vol. 17A, pp. 304–9. New York: Academic Press.
Rhodes, M. B. & Ferguson, D. L. (1973). Haemonchus contortus. Enzymes III. Glutamate dehydrogenase. Experimental Parasitology 34, 100–10.
Schwartz, M. K. (1971). Clinical aspects of arginase. In Methods in Enzymology, vol. 17B, (ed. H., Tabor, and Tabor, C. W.), pp. 857–1. New York: Academic Press.
Suda, M. & Nakagawa, M. (1971). l-Serine dehydratase (rat liver). In Methods in Enzymology, vol. 17B, (ed. H., Tabor and Tabor, C. W.), pp. 346–51. New York: Academic Press.
Wright, D. J. (1975). Studies on nitrogen catabolism in Panagrellus redivivus, Goodey, 1945 (Nematoda:Cephalobidae). Comparative Biochemistry and Physiology 52B, 255–69.
Wright, D. J. & Newall, D. R. (1976). Nitrogen excretion, osmotic and ionic regulation in nematodes. In The Organisation of Nematodes, (ed. Croll, H. A.), pp. 163210. New York: Academic Press.
Wriston, J. C. (1970). Asparaginase. In Methods in Enzymology (ed. H., Tabor and Tabor, C. W.), vol. 17 A, pp. 732–6. New York: Academic Press.


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