Skip to main content

An electrophysiological preparation of Ascaris suum pharyngeal muscle reveals a glutamate-gated chloride channel sensitive to the avermectin analogue, milbemycin D

  • R. J. Martin (a1)

An electrophysiological preparation of Ascaris suum pharyngeal muscle suitable for recording changes of input conductance using a 2-microelectrode current clamp and pharmacological study is described. The preparation is shown to contain a glutamate-gated Cl (ion sensitive) channel sensitive to the avermectin analogue, milbemycin D. The application of glutamate produces a dose-dependent increase in Cl conductance and the effect of glutamate is potentiated by milbemycin D. Milbemycin D also produced a dose-dependent increase in input conductance.

Hide All
Arena, J. P. (1994). Expression of Caenorhabditis elegans messenger-RNA in xenopus oocytes: a model system to study the mechanism of action of avermectins. Parasitology Today 10, 35–7.
Avery, L. (1993). Motor-neuron m3 controls pharyngeal muscle-relaxation timing in Caenorhabditis elegans. Journal of Experimental Biology 175, 283–97.
Avery, L., Davis, M. W., Denk, W., Dent, J. & Hess, G. (1994). M3 is an inhibitory glutamatergic pharyngeal motoneuron. Worm Breeder's Gazette 13, 72.
Byerly, L. & Masuda, M. O. (1979). Voltage-clamp analysis of the potassium current that produces a negative-going action potential in Ascaris muscle. Journal of Physiology 288, 263–84.
Campbell, W. C. & Benz, G. W. (1984). Ivermectin: a review of efficacy and safety. Journal of Veterinary Pharmacology and Therapeutics 7, 116.
Cully, D. F., Vassilatis, D. K., Liu, K. K., Paress, P. S., Vanderploeg, L. H. T. & Schaeffer, J. M. (1994). Cloning of an avermectin-sensitive glutamate-gated chloride channel from Caenorhabditis elegans. Nature, London 371, 707–11.
Del Castillo, J. & Morales, T. (1967). The electrical and mechanical activity of the esophageal cell of Ascaris lumbricoides. Journal of Generative Physiology 50, 603–30.
Geary, T. G., Sims, S. M., Thomas, E. M., Vanover, L., Davis, J. P., Winterrowd, C. A. & Klein, R. (1993). Haemonchus contortus ivermectin-induced paralysis of the pharynx. Experimental Parasitology 77, 8896.
Holden-Dye, L. & Walker, R. J. (1990). Avermectin and avermectin derivatives are antagonists at the 4-aminobutyric acid (GABA) receptors on the somatic muscle cells of Ascaris: is this the site of anthelmintic action? Parasitology 101, 265–71.
Horseman, B. G., Seymour, C., Bermudez, I. & Beadle, D. J. (1988). The effects of L-glutamate on cultured insect neurons. Neuroscience Letters 85, 6570.
Laughton, D. L., Wolstenholme, A. J. & Lunt, G. (1995). The beta subunit of the avermectin sensitive glutamate receptor is expressed in the pm4 pharyngeal muscle cells. Worm Breeder's Gazette 14, 48.
Lea, T. J. & Usherwood, P. N. R. (1973). The site of action of ibotenic acid and the identification of two populations of glutamate receptors on insect muscle-fibres. Comparative and General Pharmacology 4, 333–50.
Lingle, C. & Marder, E. (1981). A glutamate-activated chloride conductance on a crustacean muscle. Brain Research 212, 481–8.
Martin, R. J. & Pennington, A. J. (1989). A patch-clamp study of effects of dihydroavermectin on Ascaris muscle. British Journal of Pharmacology 98, 747–56.
Wafford, K. A. & Sattelle, D. B. (1989). l-glutamate receptors on the cell body membrane of an identified insect motor neuron. Journal of Experimental Biology 144, 449–62.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

  • ISSN: 0031-1820
  • EISSN: 1469-8161
  • URL: /core/journals/parasitology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *



Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed