Skip to main content
    • Aa
    • Aa
  • Get access
    Check if you have access via personal or institutional login
  • Cited by 13
  • Cited by
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Chua, Kek Heng Lim, Siew Chee Ng, Ching Ching Lee, Ping Chin Lim, Yvonne Ai Lian Lau, Tze Pheng and Chai, Hwa Chia 2015. Development of High Resolution Melting Analysis for the Diagnosis of Human Malaria. Scientific Reports, Vol. 5, p. 15671.

    Claser, Carla Malleret, Benoit Peng, Kaitian Bakocevic, Nadja Gun, Sin Yee Russell, Bruce Ng, Lai Guan and Rénia, Laurent 2014. Rodent Plasmodium-infected red blood cells: Imaging their fates and interactions within their hosts. Parasitology International, Vol. 63, Issue. 1, p. 187.

    Marie, Alexandra Boissière, Anne Tsapi, Majoline Poinsignon, Anne Awono-Ambéné, Parfait H Morlais, Isabelle Remoue, Franck and Cornelie, Sylvie 2013. Evaluation of a real-time quantitative PCR to measure the wild Plasmodium falciparum infectivity rate in salivary glands of Anopheles gambiae. Malaria Journal, Vol. 12, Issue. 1, p. 224.

    Prugnolle, F. Rougeron, V. Becquart, P. Berry, A. Makanga, B. Rahola, N. Arnathau, C. Ngoubangoye, B. Menard, S. Willaume, E. Ayala, F. J. Fontenille, D. Ollomo, B. Durand, P. Paupy, C. and Renaud, F. 2013. Diversity, host switching and evolution of Plasmodium vivax infecting African great apes. Proceedings of the National Academy of Sciences, Vol. 110, Issue. 20, p. 8123.

    Che, Pulin Cui, Long Kutsch, Olaf Cui, Liwang and Li, Qianjun 2012. Validating a Firefly Luciferase-Based High-Throughput Screening Assay for Antimalarial Drug Discovery. ASSAY and Drug Development Technologies, Vol. 10, Issue. 1, p. 61.

    Hunt, Peter W. 2011. Molecular diagnosis of infections and resistance in veterinary and human parasites. Veterinary Parasitology, Vol. 180, Issue. 1-2, p. 12.

    Polley, S. D. Boadi, S. Watson, J. Curry, A. and Chiodini, P. L. 2011. Detection and species identification of microsporidial infections using SYBR Green real-time PCR. Journal of Medical Microbiology, Vol. 60, Issue. 4, p. 459.

    Bourgeois, N. Boutet, A. Bousquet, P-J. Basset, D. Douard-Enault, C. Charachon, S. and Lachaud, L. 2010. Comparison of three real-time PCR methods with blood smears and rapid diagnostic test in Plasmodium sp. infection. Clinical Microbiology and Infection, Vol. 16, Issue. 8, p. 1305.

    Erdman, Laura K. and Kain, Kevin C. 2008. Molecular diagnostic and surveillance tools for global malaria control. Travel Medicine and Infectious Disease, Vol. 6, Issue. 1-2, p. 82.

    Berry, A. Senescau, A. Lelièvre, J. Benoit-Vical, F. Fabre, R. Marchou, B. and Magnaval, J.F. 2006. Prevalence of Plasmodium falciparum cytochrome b gene mutations in isolates imported from Africa, and implications for atovaquone resistance. Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 100, Issue. 10, p. 986.

    Magnaval, J.-F. Berry, A. Fabre, R. and Cassaing, S. 2006. Plasmodium falciparum Chloroquine-Resistance Transporter Gene Detection in Imported Plasmodium falciparum Malaria Cases. Clinical Infectious Diseases, Vol. 42, Issue. 12, p. 1806.

    Bessières, M.-H. Cassaing, S. Berry, A. Fabre, R. and Magnaval, J.-F. 2005. Apport de la biologie moléculaire au diagnostic des parasitoses. Médecine et Maladies Infectieuses, Vol. 35, p. S52.

    Berry, Antoine Vessière, Aurélia Fabre, Richard Benoit-Vical, Françoise Marchou, Bruno Massip, Patrice and Magnaval, Jean-François 2004. Pfcrt K76T mutation and its associations in imported Plasmodium falciparum malaria cases. Infection, Genetics and Evolution, Vol. 4, Issue. 4, p. 361.


Comparative assessment of conventional PCR with multiplex real-time PCR using SYBR Green I detection for the molecular diagnosis of imported malaria

  • R. FABRE (a1), A. BERRY (a1), B. MORASSIN (a1) and J. F. MAGNAVAL (a1)
  • DOI:
  • Published online: 01 January 2004

For the diagnosis of imported malaria, optical or immunochromatographic methods are known to be less sensitive and less specific than PCR-based methods, which are conversely more complicated and time-consuming. An original strategy, based upon the sequential use of a multiplex competitive real-time PCR detecting Plasmodium falciparum or Plasmodium spp. infection, followed by, if necessary, a single real-time PCR for species identification, was therefore performed and then tested versus conventional PCR in routine conditions. Conventional PCR has been used since October 1999 in the Department of Parasitology, University Hospitals in Toulouse, as a 2nd line diagnostic method. Out of 183 patients tested, 48 were found to be harbouring a falciparum infection by conventional microscopy, 60 by conventional PCR and 60 by multiplex competitive real-time PCR. Nine further patients had a non-falciparum infection, and concordant species identifications were obtained by both conventional PCR and single real-time PCR. The major value of PCR-based methods, when compared to microscopical techniques, was to ascertain the negativity of a suspect sample. Moreover, real-time PCR allows simplification of the operating procedure, with a diagnosis being made within 2 h.

Corresponding author
Tel: +33 5 61 32 28 90. Fax: +33 5 61 14 59 72. E-mail:
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

  • ISSN: 0031-1820
  • EISSN: 1469-8161
  • URL: /core/journals/parasitology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *