Skip to main content
×
Home
    • Aa
    • Aa
  • Get access
    Check if you have access via personal or institutional login
  • Cited by 67
  • Cited by
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Bernotienė, Rasa Palinauskas, Vaidas Iezhova, Tatjana Murauskaitė, Dovilė and Valkiūnas, Gediminas 2016. Avian haemosporidian parasites (Haemosporida): A comparative analysis of different polymerase chain reaction assays in detection of mixed infections. Experimental Parasitology, Vol. 163, p. 31.


    Calero-Riestra, María and García, Jesus T. 2016. Sex-dependent differences in avian malaria prevalence and consequences of infections on nestling growth and adult condition in the Tawny pipit, Anthus campestris. Malaria Journal, Vol. 15, Issue. 1,


    DUBIEC, ANNA PODMOKŁA, EDYTA ZAGALSKA-NEUBAUER, MAGDALENA DROBNIAK, SZYMON M. ARCT, ANETA GUSTAFSSON, LARS and CICHOŃ, MARIUSZ 2016. Differential prevalence and diversity of haemosporidian parasites in two sympatric closely related non-migratory passerines. Parasitology, Vol. 143, Issue. 10, p. 1320.


    Lotta, Ingrid A. Pacheco, M. Andreína Escalante, Ananias A. González, Angie D. Mantilla, Juan S. Moncada, Ligia I. Adler, Peter H. and Matta, Nubia E. 2016. Leucocytozoon Diversity and Possible Vectors in the Neotropical highlands of Colombia. Protist, Vol. 167, Issue. 2, p. 185.


    Matthews, Alix E. Ellis, Vincenzo A. Hanson, Alison A. Roberts, Jackson R. Ricklefs, Robert E. and Collins, Michael D. 2016. Avian haemosporidian prevalence and its relationship to host life histories in eastern Tennessee. Journal of Ornithology, Vol. 157, Issue. 2, p. 533.


    Moens, Michaël A.J. and Pérez-Tris, Javier 2016. Discovering potential sources of emerging pathogens: South America is a reservoir of generalist avian blood parasites. International Journal for Parasitology, Vol. 46, Issue. 1, p. 41.


    Renner, Swen C. Lüdtke, Bruntje Kaiser, Sonja Kienle, Julia Schaefer, H. Martin Segelbacher, Gernot Tschapka, Marco and Santiago-Alarcon, Diego 2016. Forests of opportunities and mischief: disentangling the interactions between forests, parasites and immune responses. International Journal for Parasitology, Vol. 46, Issue. 9, p. 571.


    Santiago-Alarcon, Diego MacGregor-Fors, Ian Kühnert, Katharina Segelbacher, Gernot and Schaefer, H. Martin 2016. Avian haemosporidian parasites in an urban forest and their relationship to bird size and abundance. Urban Ecosystems, Vol. 19, Issue. 1, p. 331.


    Václav, Radovan Betáková, Tatiana Švančarová, Petra Pérez-Serrano, Jorge Criado-Fornelio, Ángel Škorvanová, Lucia and Valera, Francisco 2016. Nest ecology of blood parasites in the European roller and its ectoparasitic carnid fly. Experimental Parasitology, Vol. 165, p. 71.


    Valkiūnas, Gediminas Ilgūnas, Mikas Bukauskaitė, Dovilė Žiegytė, Rita Bernotienė, Rasa Jusys, Vytautas Eigirdas, Vytautas Fragner, Karin Weissenböck, Herbert and Iezhova, Tatjana A. 2016. Plasmodium delichoni n. sp.: description, molecular characterisation and remarks on the exoerythrocytic merogony, persistence, vectors and transmission. Parasitology Research, Vol. 115, Issue. 7, p. 2625.


    Zagalska-Neubauer, Magdalena and Bensch, Staffan 2016. High prevalence of Leucocytozoon parasites in fresh water breeding gulls. Journal of Ornithology, Vol. 157, Issue. 2, p. 525.


    Biedrzycka, Aleksandra Migalska, Magdalena and Bielański, Wojciech 2015. A quantitative PCR protocol for detecting specific Haemoproteus lineages: molecular characterization of blood parasites in a Sedge Warbler population from southern Poland. Journal of Ornithology, Vol. 156, Issue. 1, p. 201.


    Dinhopl, Nora Nedorost, Nora Mostegl, Meike M. Weissenbacher-Lang, Christiane and Weissenböck, Herbert 2015. In situ hybridization and sequence analysis reveal an association of Plasmodium spp. with mortalities in wild passerine birds in Austria. Parasitology Research, Vol. 114, Issue. 4, p. 1455.


    Hellgren, Olof 2015. Allelic variation at innate immune genes (avian β-defensins), within a natural population of great tits. Journal of Avian Biology, Vol. 46, Issue. 1, p. 113.


    Ivanova, Karina Zehtindjiev, Pavel Mariaux, Jean and Georgiev, Boyko B. 2015. Genetic diversity of avian haemosporidians in Malaysia: Cytochrome b lineages of the genera Plasmodium and Haemoproteus (Haemosporida) from Selangor. Infection, Genetics and Evolution, Vol. 31, p. 33.


    Lotta, Ingrid A. Gonzalez, Angie D. Pacheco, M. Andreína Escalante, Ananias A. Valkiūnas, Gediminas Moncada, Ligia I. and Matta, Nubia E. 2015. Leucocytozoon pterotenuis sp. nov. (Haemosporida, Leucocytozoidae): description of the morphologically unique species from the Grallariidae birds, with remarks on the distribution of Leucocytozoon parasites in the Neotropics. Parasitology Research, Vol. 114, Issue. 3, p. 1031.


    Mata, Vanessa A. da Silva, Luís P. Lopes, Ricardo J. and Drovetski, Sergei V. 2015. The Strait of Gibraltar poses an effective barrier to host-specialised but not to host-generalised lineages of avian Haemosporidia. International Journal for Parasitology, Vol. 45, Issue. 11, p. 711.


    Pérez-Rodríguez, Antón de la Hera, Iván Bensch, Staffan and Pérez-Tris, Javier 2015. Evolution of seasonal transmission patterns in avian blood-borne parasites. International Journal for Parasitology, Vol. 45, Issue. 9-10, p. 605.


    Pérez-Rodríguez, Antón de la Hera, Iván Fernández-González, Sofía and Pérez-Tris, Javier 2014. Global warming will reshuffle the areas of high prevalence and richness of three genera of avian blood parasites. Global Change Biology, Vol. 20, Issue. 8, p. 2406.


    Hellgren, Olof Kutzer, Megan Bensch, Staffan Valkiūnas, Gediminas and Palinauskas, Vaidas 2013. Identification and characterization of the merozoite surface protein 1 (msp1) gene in a host-generalist avian malaria parasite, Plasmodium relictum (lineages SGS1 and GRW4) with the use of blood transcriptome. Malaria Journal, Vol. 12, Issue. 1, p. 381.


    ×

Diagnosing genetically diverse avian malarial infections using mixed-sequence analysis and TA-cloning

  • J. PÉREZ-TRIS (a1) and S. BENSCH (a1)
  • DOI: http://dx.doi.org/10.1017/S003118200500733X
  • Published online: 01 March 2005
Abstract

Birds harbouring several malarial parasites are common in the wild, and resolving such multiple infections is important for our understanding of host–parasite relationships. We propose a simple and reasonably accurate method for detecting and resolving multiple infections, based on the analysis of parasite cytochrome b DNA sequences: genetically mixed infections are first identified by double nucleotide peaks on sequence electropherograms, and later retrieved by TA-cloning. We applied this method to wild birds, and to experimentally created mixes with varying proportion of two parasites (Plasmodium spp. and Haemoproteus spp.). In general, the method was very efficient in detecting and resolving multiple infections, but some problems were encountered. Several multiple infections were erroneously scored as simple, either because one of the parasite lineages was a better target for the primers used, or because it was much more abundant in the mix. On the other hand, single nucleotide substitutions and template switching during PCR produced artificial sequences in some clones. We discuss the utility of the method, and propose a framework for its use when screening for genetically diverse avian malarial parasites.

Copyright
Corresponding author
Tel.: +46 46 222 37 96. Fax: +46 46 222 47 16. E-mail: javier.perez-tris@zooekol.lu.se
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Parasitology
  • ISSN: 0031-1820
  • EISSN: 1469-8161
  • URL: /core/journals/parasitology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords: