Skip to main content
×
Home
    • Aa
    • Aa

Intraerythrocytic Plasmodium falciparum utilize a broad range of serum-derived fatty acids with limited modification for their growth

  • F. MI-ICHI (a1) (a2), K. KITA (a2) and T. MITAMURA (a1)
Abstract

Plasmodium falciparum causes the most severe form of malaria. Utilization of fatty acids in serum is thought to be necessary for survival of this parasite in erythrocytes, and thus characterization of the parasite fatty acid metabolism is important in developing a new strategy for controlling malaria. Here, we examined which combinations of fatty acids present in human serum support the continuous culture of P. falciparum in serum-free medium. Metabolic labelling and gas chromatography analyses revealed that, despite the need for particular fatty acids for the growth of intraerythrocytic P. falciparum, it can metabolize a broad range of serum-derived fatty acids into the major lipid species of their membranes and lipid bodies. In addition, these analyses showed that the parasite's overall fatty acid composition reflects that of the medium, although the parasite has a limited capacity to desaturate and elongate serum-derived fatty acids. These results indicate that the Plasmodium parasite is distinct from most cells, which maintain their fatty acid composition by coordinating de novo biosynthesis, scavenging, and modification (desaturation and elongation).

Copyright
Corresponding author
Department of Molecular Protozoology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Tel: +81 6 6879 8279. Fax: +81 6 6879 8281. E-mail: mitamura@biken.osaka-u.ac.jp
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

Asahi, H., Kanazawa, T., Hirayama, N. and Kajihara, Y. ( 2005). Investigating serum factors promoting erythrocytic growth of Plasmodium falciparum. Experimental Parasitology109, 715.

Bligh, E. G. and Dyer, W. J. ( 1959). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology37, 911917.

Grellier, P., Rigomier, D., Clavey, V., Fruchart, J.-C. and Schrevel, J. ( 1991). Lipid traffic between high density proteins and Plasmodium falciparum-infected red blood cells. Journal of Cell Biology112, 267277.

Hanada, K., Mitamura, T., Fukasawa, M., Magistrado, P. A., Horii, T. and Nishijima, M. ( 2000). Neutral sphingomyelinase activity dependent on Mg2+ and anionic phospholipids in the intraerythrocytic malaria parasite Plasmodium falciparum. The Biochemical Journal346, 671677.

Heusser, D. ( 1968). Thin-layer chromatography of fatty acids on silanized silica gel. Journal of Chromatography33, 6269.

Hsiao, L. L., Howard, R. J., Aikawa, M. and Taraschi, T. F. ( 1991). Modification of host cell membrane lipid composition by the intra-erythrocytic human malaria parasite Plasmodium falciparum. The Biochemical Journal274, 121132.

Khunyoshyeng, S., Cheevadhanarak, S., Rachdawong, S. and Tanticharoen, M. ( 2002). Differential expression of desaturases and changes in fatty acid composition during sporangiospore germination and development in Mucor rouxii. Fungal Genetics and Biology37, 1321.

Krishnegowda, G. and Gowda, D. C. ( 2003). Intraerythrocytic Plasmodium falciparum incorporates extraneous fatty acids to its lipids without any structural modification. Molecular and Biochemical Parasitology132, 5558.

Mitamura, T., Hanada, K., Ko-Mitamura, E. P., Nishijima, M. and Horii, T. ( 2000). Serum factors governing intraerythrocytic development and cell cycle progression of Plasmodium falciparum. Parasitology International49, 219229.

Mitamura, T. and Palacpac, N. M. Q. ( 2003). Lipid metabolism in Plasmodium falciparum-infected erythrocyte: possible new targets for malaria chemotherapy. Microbes and Infections5, 545552.

Palacpac, N. M. Q., Hiramine, Y., Mi-ichi, F., Torii, M., Kita, K., Hiramatsu, R., Horii, T. and Mitamura, T. ( 2004). Developmental stage-specific triacylglycerol biosynthesis, degradation and trafficking as lipid bodies in Plasmodium falciparum-infected erythrocyte. Journal of Cell Science117, 14691480.

Surolia, N. and Surolia, A. ( 2001). Triclosan offers protection against blood stages of malaria by inhibiting enoyl-ACP reductase of Plasmodium falciparum. Nature, Medicine7, 167173.

Trager, W. and Jensen, J. B. ( 1976). Human malaria parasites in continuous culture. Science193, 673675.

Vial, H. J., Thuet, M. J. and Philippot, J. R. ( 1982). Phospholipid biosynthesis in synchronous Plasmodium falciparum cultures. Journal of Protozoology29, 258263.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Parasitology
  • ISSN: 0031-1820
  • EISSN: 1469-8161
  • URL: /core/journals/parasitology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords: