Britton, C. Roberts, B. and Marks, N.D. 2016. Haemonchus contortus and Haemonchosis – Past, Present and Future Trends.
Gasser, Robin B. Korhonen, Pasi K. Zhu, Xing-Quan and Young, Neil D. 2016.
Gasser, R.B. Schwarz, E.M. Korhonen, P.K. and Young, N.D. 2016. Haemonchus contortus and Haemonchosis – Past, Present and Future Trends.
Ondrovics, Martina Gasser, Robin B. and Joachim, Anja 2016.
Preston, Sarah Jabbar, Abdul and Gasser, Robin B. 2016. A perspective on genomic-guided anthelmintic discovery and repurposing using Haemonchus contortus. Infection, Genetics and Evolution, Vol. 40, p. 368.
Ratnappan, Ramesh Vadnal, Jonathan Keaney, Melissa Eleftherianos, Ioannis O’Halloran, Damien and Hawdon, John M. 2016. RNAi-mediated gene knockdown by microinjection in the model entomopathogenic nematode Heterorhabditis bacteriophora. Parasites & Vectors, Vol. 9, Issue. 1,
Wang, Meng Wang, Diandong Zhang, Xi Wang, Xu Liu, Wencui Hou, Xiaomeng Huang, Xiaoyin Xie, Bingyan and Cheng, Xinyue 2016. Double-stranded RNA-mediated interference of dumpy genes in Bursaphelenchus xylophilus by feeding on filamentous fungal transformants. International Journal for Parasitology, Vol. 46, Issue. 5-6, p. 351.
Zamanian, Mostafa and Andersen, Erik C. 2016. Prospects and challenges of CRISPR/Cas genome editing for the study and control of neglected vector-borne nematode diseases. The FEBS Journal,
Hagen, Jana Scheerlinck, Jean-Pierre Y. and Gasser, Robin B. 2015. Knocking down schistosomes – promise for lentiviral transduction in parasites. Trends in Parasitology, Vol. 31, Issue. 7, p. 324.
Hu, Dandan Song, Xingju Xie, Yue Zhong, Xiuqin Wang, Ning Zheng, Yu Gu, Xiaobin Wang, Tao Peng, Xuerong and Yang, Guangyou 2015. Molecular insights into a tetraspanin in the hydatid tapeworm Echinococcus granulosus. Parasites & Vectors, Vol. 8, Issue. 1,
Stroehlein, Andreas J. Young, Neil D. Korhonen, Pasi K. Jabbar, Abdul Hofmann, Andreas Sternberg, Paul W. and Gasser, Robin B. 2015. The Haemonchus contortus kinome - a resource for fundamental molecular investigations and drug discovery. Parasites & Vectors, Vol. 8, Issue. 1,
Tyagi, Rahul Joachim, Anja Ruttkowski, Bärbel Rosa, Bruce A. Martin, John C. Hallsworth-Pepin, Kymberlie Zhang, Xu Ozersky, Philip Wilson, Richard K. Ranganathan, Shoba Sternberg, Paul W. Gasser, Robin B. and Mitreva, Makedonka 2015. Cracking the nodule worm code advances knowledge of parasite biology and biotechnology to tackle major diseases of livestock. Biotechnology Advances, Vol. 33, Issue. 6, p. 980.
Tzelos, T. Matthews, J.B. Whitelaw, B. and Knox, D.P. 2015. Marker genes for activation of the RNA interference (RNAi) pathway in the free-living nematode Caenorhabditis elegans and RNAi development in the ovine nematode Teladorsagia circumcincta. Journal of Helminthology, Vol. 89, Issue. 02, p. 208.
Dell’Oca, Nicolás Basika, Tatiana Corvo, Ileana Castillo, Estela Brindley, Paul J. Rinaldi, Gabriel and Tort, Jose F. 2014. RNA interference in Fasciola hepatica newly excysted juveniles: Long dsRNA induces more persistent silencing than siRNA. Molecular and Biochemical Parasitology, Vol. 197, Issue. 1-2, p. 28.
Hagen, Jana Young, Neil D. Every, Alison L. Pagel, Charles N. Schnoeller, Corinna Scheerlinck, Jean-Pierre Y. Gasser, Robin B. and Kalinna, Bernd H. 2014. Omega-1 knockdown in Schistosoma mansoni eggs by lentivirus transduction reduces granuloma size in vivo. Nature Communications, Vol. 5, p. 5375.
Kotze, Andrew C. Hunt, Peter W. Skuce, Philip von Samson-Himmelstjerna, Georg Martin, Richard J. Sager, Heinz Krücken, Jürgen Hodgkinson, Jane Lespine, Anne Jex, Aaron R. Gilleard, John S. Beech, Robin N. Wolstenholme, Adrian J. Demeler, Janina Robertson, Alan P. Charvet, Claude L. Neveu, Cedric Kaminsky, Ronald Rufener, Lucien Alberich, Melanie Menez, Cecile and Prichard, Roger K. 2014. Recent advances in candidate-gene and whole-genome approaches to the discovery of anthelmintic resistance markers and the description of drug/receptor interactions. International Journal for Parasitology: Drugs and Drug Resistance, Vol. 4, Issue. 3, p. 164.
Yuan, Wang Liu, Yingying Lok, James B Stoltzfus, Jonathan D Gasser, Robin B Lei, Weiqiang Fang, Rui Zhao, Junlong and Hu, Min 2014. Exploring features and function of Ss-riok-3, an enigmatic kinase gene from Strongyloides stercoralis. Parasites & Vectors, Vol. 7, Issue. 1,
2014. Biotechnology in Medical Sciences.
Caffrey, Conor R. Britton, Collette and McKerrow, James H. 2013. Handbook of Proteolytic Enzymes.
Jex, Aaron R. Liu, Shiping Li, Bo Young, Neil D. Hall, Ross S. Li, Yingrui Geldhof, Peter Nejsum, Peter Sternberg, Paul W. Wang, Jun Yang, Huanming and Gasser, Robin B. 2013. Ascaris: The Neglected Parasite.
RNA interference (RNAi) has become an invaluable tool for the functional analysis of genes in a wide variety of organisms including the free-living nematode Caenorhabditis elegans. Recently, attempts have been made to apply this technology to parasitic helminths of animals and plants with variable success. Gene knockdown has been reported for Schistosoma mansoni by soaking or electroporating different life-stages in dsRNA. Similar approaches have been tested on parasitic nematodes which clearly showed that, under certain conditions, it was possible to interfere with gene expression. However, despite these successes, the current utility of this technology in parasite research is questionable. First, problems have arisen with the specificity of RNAi. Treatment of the parasites with dsRNA resulted, in many cases, in non-specific effects. Second, the current RNAi methods have a limited efficiency and effects are sometimes difficult to reproduce. This was especially the case in strongylid parasites where only a small number of genes were susceptible to RNAi-mediated gene knockdown. The future application of RNAi in parasite functional genomics will greatly depend on how we can overcome these difficulties. Optimization of the dsRNA delivery methods and in vitro culture conditions will be the major challenges.
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