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Epidemiological and molecular evidence supports the zoonotic transmission of Giardia among humans and dogs living in the same community

  • R. J. TRAUB (a1), P. T. MONIS (a2), I. ROBERTSON (a1), P. IRWIN (a1), N. MENCKE (a3) and R. C. A. THOMPSON (a1)...
Abstract

Giardia duodenalis isolates recovered from humans and dogs living in the same locality in a remote tea-growing community of northeast India were characterized at 3 different loci; the SSU-rDNA, elongation factor 1-alpha (ef1-α) and triose phosphate isomerase (tpi) gene. Phylogenetic analysis of the SSU-rDNA and ef1-α genes provided poor genetic resolution of the isolates within various assemblages, stressing the importance of using multiple loci when inferring genotypes to Giardia. Analysis of the tpi gene provided better genetic resolution and placed canine Giardia isolates within the genetic groupings of human isolates (Assemblages A and B). Further evidence for zoonotic transmission was supported by epidemiological data showing a highly significant association between the prevalence of Giardia in humans and presence of a Giardia-positive dog in the same household (odds ratio 3·01, 95% CI, 1·11, 8·39, P=0·0000).

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Corresponding author
WHO Collaborating Centre for the Molecular Epidemiology of Parasitic Infections, School of Veterinary and Biomedical Sciences, Murdoch University, South Street, Murdoch, Western Australia 6150. Tel: +61 (08) 93602466. Fax: +61 (08) 93104144. E-mail: andrew_t@central.murdoch.edu.au
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REFERENCES

AMAR, C. F., DEAR, P. H., PEDRAZA-DIAZ, S., LOOKER, N., LINNANE, E. & McLAUCHLIN, J. (2002). Sensitive PCR-restriction fragment length polymorphism assay for detection and genotyping of Giardia duodenalis in human faeces. Journal of Clinical Microbiology 40, 446452.
ANDERSON, T. J. C. (2001). The dangers of using single locus markers in parasite epidemiology: Ascaris as a case study. Trends in Parasitology 17, 183188.
ANDREWS, R. H., ADAMS, M., BOREHAM, P. F., MAYRHOFER, G. & MELONI, B. P. (1989). Giardia intestinalis: electrophoretic evidence for a species complex. International Journal for Parasitology 19, 183190.
ASHFORD, R. W. & SNOWDEN, K. S. (2001). Dogs and protozoan zoonoses. In Dogs, Zoonoses and Public Health (ed. Macpherson, C. N. L., Meslin, F. X. & Wandeler, A. I. ), pp. 127128. CABI Publishing, New York.
BARUCH, A. C., ISAAC-RENTON, J. & ADAM, R. D. (1996). The molecular epidemiology of Giardia lamblia: a sequence-based approach. Journal of Infectious Diseases 174, 233236.
BUGG, R. J., ROBERTSON, I. D., ELLIOT, A. D. & THOMPSON, R. C. (1999). Gastrointestinal parasites of urban dogs in Perth, Western Australia. Veterinary Journal 157, 295301.
CARRANZA, S. G., RIBERA, C., BAGUNA, J. & RIUTORT, M. (1996). Evidence that two types of 18S rDNA coexist in the genome of Dugesia (Schmidtea) mediterranea (Platyhelminthes, Turbellaria, Tricladida). Molecular Biology and Evolution 13, 824832.
DIXON, M. T. & HILLIS, D. M. (1993). Ribosomal RNA secondary structure: compensatory mutations and implications for phylogenetic analysis. Molecular Biology and Evolution 10, 256267.
EY, P. L., MANSOURI, M., KULDA, J., NOHYNKOVA, E., MONIS, P. T., ANDREWS, R. H. & MAYRHOFER, G. (1997). Genetic analysis of Giardia from hoofed farm animals reveals artiodactyl-specific and potentially zoonotic genotypes. Journal of Eukaryotic Microbiology 44, 626635.
FAUST, E. C., D'ANTONIO, J. S., ODOM, V., MILLER, M. J., PERES, C., SAWITZ, W., THOMEN, L. F., TOBIE, J. & WALKER, J. H. (1938). A critical study of clinical laboratory techniques for the diagnosis of protozoan cysts and helminth eggs in feces. American Journal of Tropical Medicine and Hygiene 18, 169183.
FRANKENA, K. & GRAAT, E. A. M. (1997). Multivariate analysis: logistic regression. In Application of Quantitative Methods in Veterinary Epidemiology (ed. Noordhuizen, J. P. T. M., Frankena, K., Van Der Hoofd, C. M. & Graat, E. A. M. ), pp. 137178. Wageningen Pers, Wageningen.
GRACZYK, T. K., BOSCO-NIZEYI, J., SSEBIDE, B., THOMPSON, R. C. A., READ, C. & CRANFIELD, M. (2002). Anthropozoonotic Giardia duodenalis Genotype (Assemblage A) infections in habitats of free-ranging human-habituated gorillas, Uganda. Journal of Parasitology 88, 905909.
HILLIS, D. M. & DIXON, M. T. (1991). Ribosomal DNA: molecular evolution and phylogenetic inference. The Quarterly Review of Biology 66, 411453.
HOMAN, W. L., VAN ENCKEVORT, F. H., LIMPER, L., VAN EYS, G. J., SCHOONE, G. J., KASPRZAK, W., MAJEWSKA, A. C. & VAN KNAPEN, F. (1992). Comparison of Giardia isolates from different laboratories by isoenzyme analysis and recombinant DNA probes. Parasitology Research 78, 316323.
HOPKINS, R. M., MELONI, B. P., GROTH, D. M., WETHERALL, J. D., REYNOLDSON, J. A. & THOMPSON, R. C. (1997). Ribosomal RNA sequencing reveals differences between the genotypes of Giardia isolates recovered from humans and dogs living in the same locality. Journal of Parasitology 83, 4451.
HOPKINS, R. M., CONSTANTINE, C. C., GROTH, D. A., WETHERALL, J. D., REYNOLDSON, J. A. & THOMPSON, R. C. (1999). PCR-based DNA fingerprinting of Giardia duodenalis isolates using the intergenic rDNA spacer. Parasitology 118, 531539.
HOSMER, D. W. & LEMESHOW, S. (1989). Applied Logistic Regression, John Wiley, New York.
ITOH, N., MURAOKA, N., AOKI, M. & ITAGAKI, T. (2001). Prevalence of Giardia lamblia infection in household dogs. Kansenshogaku Zasshi 75, 671677.
JACOBS, S. R., FORRESTER, C. P. & YANG, J. (2001). A survey of the prevalence of Giardia in dogs presented to Canadian veterinary practices. Canadian Veterinary Journal 42, 4546.
KUMAR, S., TAMURA, K., JAKOBSEN, I. B. & NEI, M. (2001). MEGA2: molecular evolutionary genetics analysis software. Arizona State University, Tempe, Arizona, USA.
LINDSAY, D. S., DUBEY, J. P., BUTLER, J. M. & BLAGBURN, B. L. (1997). Mechanical transmission of Toxoplasma gondii oocysts by dogs. Veterinary Parasitology 73, 2733.
MAYRHOFER, G., ANDREWS, R. H., EY, P. L. & CHILTON, N. B. (1995). Division of Giardia isolates from humans into two genetically distinct assemblages by electrophoretic analysis of enzymes encoded at 27 loci and comparison with Giardia muris. Parasitology 111, 1117.
McGLADE, T. R., ROBERTSON, I. D., ELLIOT, A. D. & THOMPSON, R. C. (2003). High prevalence of Giardia detected in cats by PCR. Veterinary Parasitology 110, 197205.
MELONI, B. P. & THOMPSON, R. C. (1987). Comparative studies on the axenic in vitro cultivation of Giardia of human and canine origin: evidence for intraspecific variation. Transactions of the Royal Society of Tropical Medicine and Hygiene 81, 637640.
MONIS, P. T., ANDREWS, R. H., MAYRHOFER, G. & EY, P. L. (1999). Molecular systematics of the parasitic protozoan Giardia intestinalis. Molecular Biology and Evolution 16, 11351144.
MONIS, P. T., MAYRHOFER, G., ANDREWS, R. H., HOMAN, W. L., LIMPER, L. & EY, P. L. (1996). Molecular genetic analysis of Giardia intestinalis isolates at the glutamate dehydrogenase locus. Parasitology 112, 112.
MONIS, P. T., ANDREWS, R. H., MAYRHOFER, G., MACKRILL, J., KULDA, J., ISAAC-RENTON, J. L. & EY, P. L. (1998). Novel lineages of Giardia intestinalis identified by genetic analysis of organisms isolated from dogs in Australia. Parasitology 116, 719.
MORGAN, U. M., PALLANT, L., DWYER, B. W., FORBES, D. A., RICH, G. & THOMPSON, R. C. (1998). Comparison of PCR and microscopy for detection of Cryptosporidium parvum in human faecal specimens: clinical trial. Journal of Clinical Microbiology 36, 995998.
NASH, T. E. & MOWATT, M. R. (1992). Identification and characterization of a Giardia lamblia group-specific gene. Experimental Parasitology 75, 369378.
NOLLER, H. F. (1984). Structure of ribosomal RNA. Annual Review of Biochemistry 53, 119162.
O'HANDLEY, R. M., OLSON, M. E., FRASER, D., ADAMS, P. & THOMPSON, R. C. (2000). Prevalence and genotypic characterisation of Giardia in dairy calves from Western Australia and Western Canada. Veterinary Parasitology 90, 193200.
OLIVEIRA-SEQUEIRA, T. C., AMARANTE, A. F., FERRARI, T. B. & NUNES, L. C. (2002). Prevalence of intestinal parasites in dogs from Sao Paulo State, Brazil. Veterinary Parasitology 103, 1927.
PONCE-MACOTELA, M., MARTINEZ-GORDILLO, M. N., BERMUDEZ-CRUZ, R. M., SALAZAR-SCHETTINO, P. M., ORTEGA-PIERRES, G. & EY, P. L. (2002). Unusual prevalence of the Giardia intestinalis A-II subtype amongst isolates from humans and domestic animals in Mexico. International Journal for Parasitology 32, 12011202.
QARI, S. H., GOLDMAN, I. F., PIENIAZEK, N. J., COLLINS, W. E. & LAL, A. A. (1994). Blood and sporozoite stage-specific small subunit ribosomal RNA-encoding genes of the human malaria parasite Plasmodium vivax. Gene 150, 4349.
READ, C., WALTERS, J., ROBERTSON, I. D. & THOMPSON, R. C. A. (2002). Correlation between genotype of Giardia duodenalis and diarrhoea. International Journal for Parasitology 32, 229231.
STRANDEN, A. M., ECKERT, J. & KOHLER, P. (1990). Electrophoretic characterization of Giardia isolated from humans, cattle, sheep, and a dog in Switzerland. Journal of Parasitology 76, 660668.
THOMPSON, J. D., HIGGINS, D. G. & GIBSON, T. J. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific group penalties and weight matrix choice. Nucleic Acids Research 22, 46734680.
THOMPSON, R. C. (2000). Giardiasis as a re-emerging infectious disease and its zoonotic potential. International Journal for Parasitology 30, 12591267.
THOMPSON, S. C. (1994). Giardia lamblia in children and the child care setting: a review of the literature. Journal of Paediatrics and Child Health 30, 202209.
TRAUB, R. J., ROBERTSON, I. D., IRWIN, P., MENCKE, N. & THOMPSON, R. C. (2002). The role of dogs in transmission of gastrointestinal parasites in a remote tea-growing community in northeastern India. American Journal of Tropical Medicine and Hygiene 67, 539545.
world health organization (1996). The World Health Report 1996. Fighting Disease, Fostering Development. WHO, Geneva.
YONG, T. S., PARK, S. J., HWANG, U. W., YANG, H. W., LEE, K. W., MIN, D. Y., RIM, H. J., WANG, Y. & ZHENG, F. (2000). Genotyping of Giardia lamblia isolates from humans in China and Korea using ribosomal DNA sequences. Journal of Parasitology 86, 887891.
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Parasitology
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