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Genetic diversity of schistosomes and snails: implications for control


Molecular approaches are providing new insights into the genetic diversity of schistosomes and their intermediate snail hosts. For instance, molecular tools based on the polymerase chain reaction are being developed for the diagnosis of schistosomiasis and the detection of prepatent schistosome infections in snails at transmission sites. Robust phylogenies of the different species of Schistosoma, Bulinus and Biomphalaria have been determined and novel methods are available to identify the different and cryptic taxa involved. Microsatellite analyses and mitochondrial DNA sequencing methods have been developed and are contributing to a better understanding of the genetic structure of both schistosome and snail populations. New sampling procedures to capture DNA of eggs and larval stages of schistosomes in field situations are facilitating more detailed and ethically advantageous studies on parasite heterogeneity. Knowledge of the genetic diversity of schistosome and snail populations adds a further dimension to the monitoring and surveillance of disease, and the implementation of new molecular-based approaches will be of increasing importance in helping to assess the impact of schistosomiasis control strategies.

Corresponding author
*Corresponding author: David Rollinson, Wolfson Wellcome Biomedical Laboratories, Department of Zoology, Natural History Museum, Cromwell Road, LondonSW7 5BD, UK. Tel: +44 20 7942 518. Fax: +44 20 7942 5518. E-mail:
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L. E. Agola , D. N. Mburu , R. J. DeJong , B. N. Mungai , G. M. Muluvi , E. N. Njagi , E. S. Loker and G. M. Mkoji (2006). Microsatellite typing reveals strong genetic structure of Schistosoma mansoni from localities in Kenya. Infection Genetics and Evolution 6, 484490.

ANON, (2007). A turning point for neglected tropical disease control. Lancet 369, 1404.

J. Balen , J. R. Stothard , N. B. Kabatereine , E. M. Tukahebwa , F. Kazibwe , S. Whawell , J. P. Webster , J. Utzinger and A. Fenwick (2006). Morbidity due to Schistosoma mansoni: an epidemiological assessment of distended abdomen syndrome in Ugandan school children with observations before and 1-year after anthelminthic chemotherapy. Transactions of the Royal Society of Tropical Medicine and Hygiene 100, 10391048.

L. Blair , J. P. Webster and G. C. Barker (2001). Isolation and characterization of polymorphic microsatellite markers in Schistosoma mansoni from Africa. Molecular Ecology Notes 1, 9395.

H. O. Bogh , X. Q. Zhu , B. Z. Qian and R. B. Gasser (1999). Scanning for nucleotide variations in mitochondrial DNA fragments of Schistosoma japonicum by single-strand conformation polymorphism. Parasitology 118, 7382.

P. Boisier , C. E. Ramarokoto , P. Ravoniarimbinina , L. Rabarijaona and V. E. Ravaoalimalala (2001). Geographic differences in hepatosplenic complications of schistosomiasis mansoni and explanatory factors of morbidity. Tropical Medicine and International Health 6, 699706.

S. V. Brant and E. S. Loker (2005). Can specialized pathogens colonize distantly related hosts? Schistosome evolution as a case study. PLoS Pathogens 1, 167169.

C. M. Davies , J. P. Webster and M. E. J. Woolhouse (2001). Trade-offs in the evolution of virulence in an indirectly transmitted macroparasite. Proceedings of the Royal Society of London Series B-Biological Sciences 268, 251257.

P. Durand , C. Sire and A. Theron (2000). Isolation of microsatellite markers in the digenetic trematode Schistosoma mansoni from Guadeloupe island. Molecular Ecology 9, 997998.

R. B. Gasser (1998). What's in that band? International Journal for Parasitology 28, 989996.

R. Golan , C. M. Gower , A. M. Emery , D. Rollinson , and J. P. Webster (2008). Isolation and characterization of the first polymorphic microsatellite markers for Schistosoma haematobium and their application in multiplex reactions. Molecular Ecology Resources 8, 647649.

A. L. Gomes , F. L. Melo , R. P. Werkhauser and F. G. Abath (2006). Development of a real time polymerase chain reaction for quantitation of Schistosoma mansoni DNA. Memorias do Instituto Oswaldo Cruz 101 (Suppl 1), 133136.

A. Jørgensen , T. K. Kristensen and J. R. Stothard (2007). Phylogeny and biogeography of African Biomphalaria (Gastropoda: Planorbidae) with special emphasis on the endemic species of the great East African lakes. Zoological Journal of the Linnean Society 151, 337349.

E. Jolly , C. Chin , S. Miller , M. Baghat , K. Lim , J. DeRisis and J. McKerrow (2007). Gene expression patterns during adaptation of a parasite helminth to different environmental niches. Genome Biology 8, R65.

R. A. Kane , J. Bartley , J. R. Stothard , J. Vercruysse , D. Rollinson and V. R. Southgate (2002). Application of single strand conformational polymorphism (SSCP) analysis with fluorescent primers for differentiation of Schistosoma haematobium group species. Transactions of the Royal Society of Tropical Medicine and Hygiene 96, 235241.

R. A. Kane , J. R. Stothard , A. M. Emery and D. Rollinson (2008). Molecular characterization of freshwater snails in the genus Bulinus: a role for barcodes? Parasites and Vectors 1:15 doi:10.1186/1756-3305-1-15.

C. H. King , R. F. Sturrock , H. C. Kariuki and J. Hamburger (2006). Transmission control for schistosomiasis – why it matters now. Trends in Parasitology 22, 575582.

T. H. Le , D. Blair and D. P. McManus (2000). Mitochondrial DNA sequences of human schistosomes: the current status. International Journal for Parasitology 30, 283290.

T. H. Le , D. Blair and D. P. McManus (2002 a). Revisiting the question of limited genetic variation within Schistosoma japonicum. Annals of Tropical Medicine and Parasitology 96, 155164.

T. H. Le , D. Blair and D. P. McManus (2002 b). Mitochondrial genomes of parasitic flatworms. Trends in Parasitology 18, 206213.

T. H. Le , P. F. Humair , D. Blair , T. Agatsuma , D. T. Littlewood and D. P. McManus (2001). Mitochondrial gene content, arrangement and composition compared in African and Asian schistosomes. Molecular and Biochemical Parasitology 117, 6171.

D. T. Littlewood , A. E. Lockyer , B. L. Webster , D. A. Johnston and T. H. Le (2006). The complete mitochondrial genomes of Schistosoma haematobium and Schistosoma spindale and the evolutionary history of mitochondrial genome changes among parasitic flatworms. Molecular Phylogenetics and Evolution 39, 452467.

A. E. Lockyer , J. Spinks , L. R. Noble , D. Rollinson and C. S. Jones (2007). Identification of genes involved in interactions between Biomphalaria glabrata and Schistosoma mansoni by suppression subtractive hybridization. Molecular and Biochemical Parasitology 151, 1827.

F. L. Melo , A. L. Gomes , C. S. Barbosa , R. P. Werkhauser and F. G. Abath (2006). Development of molecular approaches for the identification of transmission sites of schistosomiasis. Transactions of the Royal Society of Tropical Medicine and Hygiene 100, 10491055.

J. A. Morgan , R. J. Dejong , G. O. Adeoye , E. D. Ansa , C. S. Barbosa , P. Bremond , I. M. Cesari , N. Charbonnel , L. R. Correa , G. Coulibaly , P. S. D'Andrea , C. P. De Souza , M. J. Doenhoff , S. File , M. A. Idris , R. N. Incani , P. Jarne , D. M. Karanja , F. Kazibwe , J. Kpikpi , N. J. Lwambo , A. Mabaye , L. A. Magalhaes , A. Makundi , H. Mone , G. Mouahid , G. M. Muchemi , B. N. Mungai , M. Sene , V. R. Southgate , L. A. T. Tchuente , A. Theron , F. Yousif , E. M. Zanotti-Magalhaes , G. M. Mkoji and E. S. Loker (2005). Origin and diversification of the human parasite Schistosoma mansoni. Molecular Ecology 14, 38893902.

J. A. Morgan , R. J. DeJong , F. Kazibwe , G. M. Mkoji and E. S. Loker (2003). A newly-identified lineage of Schistosoma. International Journal for Parasitology 33, 977985.

F. Prugnolle , T. De Meeus , P. Durand , C. Sire and A. Theron (2002). Sex-specific genetic structure in Schistosoma mansoni: evolutionary and epidemiological implications. Molecular Ecology 11, 12311238.

F. Prugnolle , A. Theron , P. Durand and T. De Meeus (2004). Test of pangamy by genetic analysis of Schistosoma mansoni pairs within its natural murine host in Guadeloupe. Journal of Parasitology 90, 507509.

R. J. Quinnell (2003). Genetics of susceptibility to human helminth infection. International Journal for Parasitology 33, 12191231.

N. B. Rodrigues , P. Coura Filho , C. P. de Souza , L. K. Jannoti Passos , E. Dias-Neto and A. J. Romanha (2002). Populational structure of Schistosoma mansoni assessed by DNA microsatellites. International Journal for Parasitology 32, 843851.

D. Rollinson , J. R. Stothard and V. R. Southgate (2001). Interactions between intermediate snail hosts of the genus Bulinus and schistosomes of the Schistosoma haematobium group. Parasitology 123, S245S260.

J. W. Rudge , H. Carabin , E. Balolong Jr., V. Tallo , J. Shrivastava , D-B Lu , M-G Basáñez , R. Olveda , S. T. McGarvey and J. P. Webster (2008). Population genetics of Schistosoma japonicum within the Philippines suggest high levels of transmission between humans and dogs. PloS Neglected Tropical Diseases 2: e340.

J. W. Rudge , D-B Lu , G-W Feng , T-P Wang , M-G. Basáñez and J. P. Webster (2009). Parasite genetic differentiation by host species and habitat type: molecular epidemiology of Schistosoma japonicum in hilly and marshland areas of Anhui Province, China. Molecular Ecology 18, 2134–47.

N. Sandoval , M. Siles-Lucas , J. Lopez Aban , J. L. Perez-Arellano , T. Garate and A. Muro (2006 a). Schistosoma mansoni: a diagnostic approach to detect acute schistosomiasis infection in a murine model by PCR. Experimental Parasitology 114, 8488.

J. Shrivastava , G. C. Barker , M. V. Johansen , Z. Xiaonong , G. D. Aligui , S. T. McGarvey and J. P. Webster (2003). Isolation and characterization of polymorphic DNA microsatellite markers from Schistosoma japonicum. Molecular Ecology Notes 3, 406408.

M. L. Steinauer , L. E. Agola , I. N. Mwangi , G. M. Mkoji and E. S. Loker (2008 a). Molecular epidemiology of Schistosoma mansoni: A robust, high-throughput method to assess multiple microsatellite markers from individual miracidia. Infection, Genetics and Evolution 8, 6873.

M. L. Steinauer , B. Hanelt , I. N. Mwangi , G. M. Maina , L. E. Agola , J. M. Kinthuia , M. W. Mutuku , B. N. Mungal , W. D. Wilson , G. M. Mkoji and E. S. Loker (2008 b). Introgressive hybridization of human and rodent schistosome parasites in western Kenya. Molecular Ecology 17, 50625074.

J. R. Stothard , J. Bartley , C. S. Griffin , S. J. Hubbard , T. K. Kristensen and D. Rollinson (2002 a). Identification of snails within the Bulinus africanus group from East Africa by multiplex SNaPshot™ analysis of single nucleotide polymorphisms within the cytochrome oxidase subunit I. Memorias do Instituto Oswaldo Cruz. 97, 3136.

J. R. Stothard and A. F. Gabrielli (2007). Schistosomiasis in African infants and preschool children: to treat or not to treat? Trends in Parasitology 23, 8386.

J. R. Stothard , A. F. Mgeni , S. Khamis , E. Seto , M. Ramsan , S. J. Hubbard , T. K. Kristensen and D. Rollinson (2002 b). New insights into the transmission biology of urinary schistosomiasis in Zanzibar. Transactions of the Royal Society of Tropical Medicine and Hygiene 96, 470475.

T. Suzuki , Y. Osada , T. Kumagai , A. Hamada , E. Okuzawa and T. Kanazawa (2006). Early detection of Schistosoma mansoni infection by touchdown PCR in a mouse model. Parasitology International 55, 213218.

R. J. Ten Hove , J. C. Verweij , K. Vereecken , K. Polman , L. Dieye and L. van Lieshout (2008). Multiplex real-time PCR for the detection and quantification of Schistosoma mansoni and S. haematobium infection in stool samples collected in northern Senegal. Transactions of the Royal Society of Tropical Medicine and Hygiene 102, 179185.

K. K. Van de Vijver , C. G. Colpaert , W. Jacobs , K. Kuypers , C. H. Hokke , A. M. Deelder and E. A. Van Marck (2006). The host's genetic background determines the extent of angiogenesis induced by schistosome egg antigens. Acta Tropica 99, 243251.

T. P. Wang , J. Shrivastava , M. V. Johansen , S. Q. Zhang , F. F. Wang and J. P. Webster (2006). Does multiple hosts mean multiple parasites? Population genetic structure of Schistosoma japonicum between definitive host species. International Journal for Parasitology 36, 13171325.

B. L. Webster , V. R. Southgate and D. T. Littlewood (2006). A revision of the interrelationships of Schistosoma including the recently described Schistosoma guineensis. International Journal for Parasitology 36, 947955.

B. L. Webster , L. A. T. Tchuente and V. R. Southgate (2007). A single-strand conformation polymorphism (SSCP) approach for investigating genetic interactions of Schistosoma haematobium and Schistosoma guineensis in Loum, Cameroon. Parasitology Research 100, 739745.

R. A. Wilson , P. D. Ashton , S. Braschi , G. P. Dillon , M. Berriman and A. Ivens (2007). ‘Oming’ in on schistosomes: prospects and limitations for post-genomics. Trends in Parasitology 23, 1420.

M. Zarowiecki , T. Huyse and D. T. J. Littlewood (2007). Making the most of mitochondrial genomes – markers for phylogeny, molecular ecology and barcodes in Schistosoma (Platyhelminthes: Digenea). International Journal for Parasitology 37, 14011418.

S. M. Zhang , C. M. Adema , T. B. Kepler and E. S. Loker (2004). Diversification of Ig superfamily genes in an invertebrate. Science 305, 251254.

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