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Prevalence of infection and 18S rRNA gene sequences of Cytauxzoon species in Iberian lynx (Lynx pardinus) in Spain

  • J. MILLÁN (a1), V. NARANJO (a2), A. RODRÍGUEZ (a1), J. M. PÉREZ DE LA LASTRA (a2), A. J. MANGOLD (a3) and J. DE LA FUENTE (a2) (a4)
  • DOI:
  • Published online: 28 February 2007

The Iberian lynx (Lynx pardinus) is the most endangered felid in the world. Only about 160 individuals remain in 2 separate metapopulations in Southern Spain (Sierra Morena and Doñana). We obtained blood samples of 20 lynxes captured from 2004 to 2006, and determined the prevalence of infection and genetic diversity of Cytauxzoon spp. using 18S rRNA PCR and sequence analysis. Prevalence of infection was 15% (3 of 20). Cytauxzoon sp. was only detected in Sierra Morena. For phylogenetic analysis, we used the sequences reported in the present study and those characterized in different domestic and wild felids and ticks from North and South America, Asia and Europe. Three different Cytauxzoon sp. sequences were obtained. They were closely related to that obtained from a Spanish cat, but diverged in up to 1·0% with respect to the only previously reported sequence from an Iberian lynx. Conversely, the latter sequence clustered together with C. manul sequences obtained from Pallas cats (Otocolobus manul) in Mongolia. Our analysis yields a separate cluster of C. felis sequences from cats, wild felids and ticks in the United States and Brazil. These results suggest that at least 2 different Cytauxzoon spp. may be present in Iberian lynx. The apparent absence in one of the areas, together with the possibility of fatal cytauxzoonosis in lynxes makes necessary disease risks to be taken into account in management conservation strategies, such as translocations and re-introductions.

Corresponding author
*Corresponding author: Estación Biológica de Doñana (CSIC), Pabellón del Perú, Avda. María Luisa s/n, 41013-Sevilla, Spain. Tel: +34 954323240. Fax: +34 954621125. E-mail:
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M. T. Allsopp , T. Cavalier-Smith , D. T. De Waal and B. A. Allsopp (1994). Phylogeny and evolution of the piroplasms. Parasitology 108, 147152.

S. F. Altschul , W. Gish , W. Miller , E. W. Myers and D. J. Lipman (1990). Basic local alignment search tool. Journal of Molecular Biology 215, 403410.

P. J. Bondy Jr., L. A. Cohn , J. W. Tyler and A. E. Marsh (2005). Polymerase chain reaction detection of Cytauxzoon felis from field-collected ticks and sequence analysis of the small subunit and internal transcribed spacer 1 region of the ribosomal RNA gene. Journal of Parasitology 91, 458461.

A. J. Birkenheuer , J. A. Le , A. M. Valenzisi , M. D. Tucker , M. G. Levy and E. B. Breitschwerdt (2006 a). Cytauxzoon felis infection in cats in the mid-Atlantic states: 34 cases (1998–2004). Journal of the American Veterinary Medicine Association 228, 568571.

A. J. Birkenheuer , H. Marr , A. R. Alleman , M. G. Levy and E. B. Breitschwerdt (2006 b). Development and evaluation of a PCR assay for the detection of Cytauxzoon felis DNA in feline blood samples. Veterinary Parasitology 137, 144149.

E. F. Blouin , A. A. Kocan , K. M. Kocan and J. Hair (1987). Evidence of a limited schizogonous cycle for Cytauxzoon felis in bobcats following exposure to infected ticks. Journal of Wildlife Diseases 23, 499501.

A. Criado-Fornelio , M. A. González del , A. Buling-Saraña and J. C. Barba-Carretero (2004). The “expanding universe” of piroplasms. Veterinary Parasitology 119, 337345.

F. de Castro and B. Bolker (2005). Mechanisms of disease-induced extinction. Ecology Letters 8, 117126.

S. L. Deem , W. B. Karesh and W. Weisman (2001). Putting theory into practice: wildlife health in conservation. Conservation Biology 15, 12241233.

J. Felsenstein (1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783791.

P. Ferreras , M. Delibes , F. Palomares , J. M. Fedriani , J. Calzada and E. Revilla (2004). Dispersal in the Iberian lynx: factors affecting the start, duration, distance and dispersal success. Behavioral Ecology 15, 3140.

G. E. Fox , J. D. Wisotzkey and P. Jurtshuk Jr. (1992). How close is close: 16S rRNA sequence identity may not be sufficient to guarantee species identity. International Journal of Systematic Bacteriology 42, 166170.

M. M. Garner , N. P. Lung , S. Citino , E. C. Greiner , J. W. Harvey and B. L. Homer (1996). Fatal cytauxzoonosis in a captive-reared white tiger (Panthera tigris). Veterinary Pathology 33, 8286.

D. T. Haydon , M. K. Laurenson and C. Sillero-Zubiri (2002). Integrating epidemiology into population viability analysis: managing the risk posed by rabies and canine distemper to the Ethiopian wolf. Conservation Biology 16, 13721385.

C. J. Ketz-Riley , M. V. Reichard , R. A. Van den Bussche , J. P. Hoover , J. Meinkoth and A. A. Kocan (2003). An intraerythrocytic small piroplasm in wild-caught Pallas's cats (Otocolobus manul) from Mongolia. Journal of Wildlife Diseases 39, 424430.

A. A. Kocan , E. F. Blouin and B. L. Glenn (1985). Hematologic and serum chemical values for free-ranging bobcats, Felis rufus (Schreber), with reference to animals with natural infections of Cytauxzoon felis Kier, 1979. Journal of Wildlife Diseases 21, 190192.

S. Kumar , K. Tamura and M. Nei (2004). MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Briefings in Bioinformatics 5, 150163.

I. Luaces , E. Aguirre , M. García-Montijano , J. Velarde , M. A. Tesouro , C. Sánchez , M. Galka , P. Fernández and A. Sainz (2005). First report of an intraerythrocytic small piroplasm in wild Iberian lynx (Lynx pardinus). Journal of Wildlife Diseases 41, 810815.

F. Mathews , D. Moro , R. Strachan , M. Gelling and N. Buller (2006). Health surveillance in wildlife reintroductions. Biological Conservation 131, 338347.

J. H. Meinkoth and A. A. Kocan (2005). Feline cytauxzoonosis. Veterinary Clinics of North America: Small Animal Practice 35, 89101.

J. Meinkoth , A. A. Kocan , L. Whitworth , G. Murphy , J. C. Fox and J. P. Woods (2000). Cats surviving natural infection with Cytauxzoon felis: 18 cases (1997–1998). Journal of Veterinary Internal Medicine 14, 521525.

J. C. Nietfeld and C. Pollock (2002). Fatal cytauxzoonosis in a free-ranging bobcat (Lynx rufus). Journal of Wildlife Diseases 38, 607610.

F. Palomares , A. Rodríguez , R. Laffitte and M. Delibes (1991). The status and distribution of the Iberian lynx Felis pardina (Temminck) in Coto Doñana area, SW Spain. Biological Conservation 57, 159169.

J. M. Pérez and R. L. Palma (2001). A new species of Felicola (Phthiraptera: Trichodectidae) from the endangered Iberian lynx: another reason to ensure its survival. Biodiversity and Conservation 10, 929937.

M. V. Reichard , R. A. Van den Bussche , J. H. Meinkoth , J. P. Hoover and A. A. Kocan (2005). A new species of Cytauxzoon from Pallas' cats caught in Mongolia and comments on the systematics and taxonomy of piroplasmids. Journal of Parasitology 91, 420426.

A. Rodríguez and M. Delibes (2002). Internal structure and patterns of contraction in the geographic range of the Iberian lynx. Ecography 25, 314328.

D. S. Rotstein , S. K. Taylor , J. W. Harvey and J. Bean (1999). Hematologic effects of cytauxzoonosis in Florida panthers and Texas cougars in Florida. Journal of Wildlife Diseases 35, 613617.

M. E. Scott (1988). The impact of infectious disease on animal populations: implications for conservation biology. Conservation Biology 2, 4065.

K. F. Smith , D. F. Sax and K. D. Lafferty (2006). Evidence for the role of infectious disease in species extinction and endangerment. Conservation Biology 20, 13491357.

J. D. Thompson , D. G. Higgins and T. J. Gibson (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 46734680.

E. T. Thorne and E. S. Williams (1988). Disease and endangered species: the black-footed ferret as a recent example. Conservation Biology 2, 6674.

M. J. Yabsley , S. M. Murphy and M. W. Cunningham (2006). Molecular detection and characterization of Cytauxzoon felis and a Babesia species in cougars from Florida. Journal of Wildlife Diseases 42, 366374.

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