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Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non-endemic regions of Argentina

  • C. I. CURA (a1), R. H. LUCERO (a2), M. BISIO (a1), E. OSHIRO (a3), L. B. FORMICHELLI (a2), J. M. BURGOS (a4), S. LEJONA (a5), B. L. BRUSÉS (a2), D. O. HERNÁNDEZ (a6), G. V. SEVERINI (a6), E. VELAZQUEZ (a7), T. DUFFY (a1), E. ANCHART (a5), R. LATTES (a8), J. ALTCHEH (a9), H. FREILIJ (a9), M. DIEZ (a10), C. NAGEL (a10), C. VIGLIANO (a10), L. FAVALORO (a10), R. R. FAVALORO (a10), D. E. MERINO (a2), S. SOSA-ESTANI (a3) (a7) and A. G. SCHIJMAN (a1)...


Genetic diversity of Trypanosoma cruzi may play a role in pathogenesis of Chagas disease forms. Natural populations are classified into 6 Discrete Typing Units (DTUs) Tc I-VI with taxonomical status. This study aimed to identify T. cruzi DTUs in bloodstream and tissue samples of Argentinean patients with Chagas disease. PCR-based strategies allowed DTU identification in 256 clinical samples from 239 Argentinean patients. Tc V prevailed in blood from both asymptomatic and symptomatic cases and Tc I was more frequent in bloodstream, cardiac tissues and chagoma samples from immunosuppressed patients. Tc II and VI were identified in a minority of cases, while Tc III and Tc IV were not detected in the studied population. Interestingly, Tc I and Tc II/VI sequences were amplified from the same skin biopsy slice from a kidney transplant patient suffering Chagas disease reactivation. Further data also revealed the occurrence of mixed DTU populations in the human chronic infection. In conclusion, our findings provide evidence of the complexity of the dynamics of T. cruzi diversity in the natural history of human Chagas disease and allege the pathogenic role of DTUs I, II, V and VI in the studied population.


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*Corresponding author: Vuelta de Obligado 2490, Buenos Aires 1428, Argentina. Tel: +54 11 47832871. Fax: +54 11 47868576. E-mail:


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Bisio, M., Cura, C., Duffy, T., Altcheh, J., Giganti, S. O., Begher, S., Scapellato, P., Burgos, J. M., Levin, M. J., Schreck, R., Freilij, H. and Schijman, A. G. (2009). Trypanosoma cruzi discrete typing units in Chagas disease patients with HIV co-infection. Revista Biomédica 20, 166178.
Brenière, S. F., Bosseno, M. F., Telleria, J., Bastrenta, B., Yacsik, N., Noireau, F., Alcazar, J. L., Barnabé, C., Wincker, P. and Tibayrenc, M. (1998). Different behavior of two Trypanosoma cruzi major clones: transmission and circulation in young Bolivian patients. Experimental Parasitology 89, 285295.
Burgos, J. M., Altcheh, J., Bisio, M., Duffy, T., Valadares, H. M., Seidenstein, M. E., Piccinali, R., Freitas, J. M., Levin, M. J., Macchi, L., Macedo, A. M., Freilij, H. and Schijman, A. G. (2007). Direct molecular characterization of bloodstream parasite populations causing Congenital Chagas disease. International Journal for Parasitology 37, 13191327.
Burgos, J. M., Begher, S. B., Freitas, J. M., Bisio, M., Duffy, T., Altcheh, J., Teijeiro, R., Lopez Alcoba, H., Deccarlini, F., Freilij, H., Levin, M. J., Levalle, J., Macedo, A. M. and Schijman, A. G. (2005). Molecular diagnosis and typing of Trypanosoma cruzi populations and lineages in cerebral Chagas disease in a patient with AIDS. The American Journal of Tropical Medicine and Hygiene 73, 10161018.
Burgos, J. M., Diez, M., Vigliano, C., Bisio, M., Risso, M., Duffy, T., Cura, C., Brusses, B., Favaloro, L., Leguizamon, M. S., Lucero, R. H., Laguens, R., Levin, M. J., Favaloro, R. and Schijman, A. G. (2010). Molecular identification of Trypanosoma cruzi discrete typing units in end-stage chronic Chagas heart disease and reactivation after heart transplantation. Clinical Infectious Diseases 51, 485495.
Campbell, D. A., Westenberger, S. J. and Sturm, N. R. (2004). The determinants of Chagas disease: connecting parasite and host genetics. Current Molecular Medicine 4, 549562.
Carranza, J. C., Valadares, H. M., D'Avila, D. A., Baptista, R. P., Moreno, M., Galvão, L. M., Chiari, E., Sturm, N. R., Gontijo, E. D., Macedo, A. M. and Zingales, B. (2009). Trypanosoma cruzi maxicircle heterogeneity in Chagas disease patients from Brazil. International Journal for Parasitology 39, 963973.
Di Noia, J. M., Buscaglia, C. A., De Marchi, C. R., Almeida, I. C. and Frasch, A. C. (2002). A Trypanosoma cruzi small surface molecule provides the first immunological evidence that Chagas' disease is due to a single parasite lineage. The Journal of Experimental Medicine 195, 401413.
Diosque, P., Barnabe, C., Padilla, A., Marco, J., Cardozo, R., Cimino, R., Nasserd, J., Tibayrenc, M. and Basombrıo, M. (2003). Multilocus enzyme electrophoresis analysis of Trypanosoma cruzi isolates from a geographically restricted endemic area for Chagas’ disease in Argentina. International Journal for Parasitology 33, 9971003.
Duffy, T., Bisio, M., Altcheh, J., Burgos, J. M., Diez, M., Levin, M. J., Favaloro, R. R., Freilij, H. and Schijman, A. G. (2009). Accurate real-time PCR strategy for monitoring bloodstream parasitic loads in chagas disease patients. PLoS Neglected Tropical Diseases 3, e419.
Freitas, J. M., Lages-Silva, E., Crema, E., Pena, S. D. and Macedo, A. M. (2005). Real time PCR strategy for the identification of major lineages of Trypanosoma cruzi directly in chronically infected human tissues. International Journal for Parasitology 35, 411417.
Gaunt, M. W., Yeo, M., Frame, I. A., Stothard, J. R., Carrasco, H. J., Taylor, M. C., Mena, S. S., Veazey, P., Miles, G. A., Acosta, N., de Arias, A. R. and Miles, M. A. (2003). Mechanism of genetic exchange in American trypanosomes. Nature, London 421, 936939.
Higo, H., Miura, S., Horio, M., Mimori, T., Hamano, S., Agatsuma, T., Yanagi, T., Cruz-Reyes, A., Uyema, N., Rojas de Arias, A., Matta, V., Akahane, H., Hirayama, K., Takeuchi, T., Tada, I. and Himeno, K. (2004). Genotypic variation among lineages of Trypanosoma cruzi and its geographic aspects. Parasitology International 53, 337344.
Macedo, A. M. and Pena, S. D. J. (1998). Genetic variability of Trypanosoma cruzi: implications for the pathogenesis of Chagas disease. Parasitology Today 14, 119123.
Macedo, A. M., Machado, C. R., Oliveira, R. P. and Pena, S. D. (2004). Trypanosoma cruzi: genetic structure of populations and relevance of genetic variability to the pathogenesis of chagas disease. Memórias do Instituto Oswaldo Cruz 99, 112.
Miles, M. A., Llewellyn, M. S., Lewis, M. D., Yeo, M., Baleela, R., Fitzpatrick, S., Gaunt, M. W. and Mauricio, I. L. (2009). The molecular epidemiology and phylogeography of Trypanosoma cruzi and parallel research on Leishmania: looking back and to the future. Parasitology 136, 15091528.
Ocaña-Mayorga, S., Llewellyn, M. S., Costales, J. A., Miles, M. A. and Grijalva, M. J. (2010). Sex, subdivision, and domestic dispersal of Trypanosoma cruzi lineage I in southern Ecuador. PLoS Neglected Tropical Diseases 4, e915.
Vago, A. R., Andrade, L. O., Leite, A. A., d'Avila Reis, D., Macedo, A. M., Adad, S. J., Tostes, S. Jr, Moreira, M. C., Filho, G. B. and Pena, S. D. (2000). Genetic characterization of Trypanosoma cruzi directly from tissues of patients with chronic Chagas disease: differential distribution of genetic types into diverse organs. The American Journal of Pathology 156, 18051809.
Virreira, M., Alonso-Vega, C., Solano, M., Jijena, J., Brutus, L., Bustamante, Z., Truyens, C., Schneider, D., Torrico, F., Carlier, Y. and Svoboda, M. (2006). Congenital Chagas disease in Bolivia is not associated with DNA polymorphism of Trypanosoma cruzi. The American Journal of Tropical Medicine and Hygiene 75, 871879.
World Health Organization (2007). Report of the Scientific Working Group on Chagas disease. TDR/SWG/09. World Health Organization, Geneva, Switzerland.
Zingales, B., Andrade, S. G., Briones, M. R., Campbell, D. A., Chiari, E., Fernandes, O., Guhl, F., Lages-Silva, E., Macedo, A. M., Machado, C. R., Miles, M. A., Romanha, A. J., Sturm, N. R., Tibayrenc, M. and Schijman, A. G. (2009). A new consensus for Trypanosoma cruzi intraspecific nomenclature: second revision meeting recommends TcI to TcVI. Memórias do Instituto Oswaldo Cruz 104, 10511054.



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