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ALS resistance in several smooth pigweed (Amaranthus hybridus) biotypes

  • Cory M. Whaley (a1), Henry P. Wilson and James H. Westwood (a2)
Abstract

Experiments were conducted to identify acetolactate synthase (ALS, EC 2.2.1.6 [formerly EC 4.1.3.18]) mutation sites in eight biotypes of smooth pigweed and correlate these mutations with patterns of herbicide cross-resistance. Four herbicide-resistant smooth pigweed biotypes (R5, R6, R7, R8) collected from fields in Virginia, Delaware, and Maryland, showed a similar response to postemergence applications of the ALS-inhibitors imazethapyr, pyrithiobac, chlorimuron, thifensulfuron, and cloransulam. These R biotypes ranged from 261- to 537-fold resistant to imazethapyr and 29- to 88-fold resistant to pyrithiobac. The biotypes also had reduced sensitivity to chlorimuron and thifensulfuron of 2- to 14-fold and 10- to 25-fold, respectively, relative to a susceptible smooth pigweed biotype (S). Biotypes R6, R7, and R8 had reduced sensitivity of 3- to 10-fold to cloransulam relative to the S biotype, whereas R5 had increased sensitivity. All of these biotypes were found to have a serine to asparagine substitution at amino acid position 653, as numbered relative to the protein sequence of Arabidopsis thaliana. This stands in contrast to four other imidazolinone (IMI)-resistant smooth pigweed biotypes (R1, R2, R3, R4) that were collected from fields in Somerset County, Maryland. These biotypes were found to have an alanine to threonine substitution at position 122 of the ALS enzyme and were previously characterized at the whole-plant level with high-level resistance to IMI herbicides, increased sensitivity to pyrimidinylthiobenzoate and triazolopyrimidine sulfonanilide herbicides, and low to no cross-resistance to sulfonylurea herbicides.

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Corresponding author
Corresponding author. Eastern Shore Agricultural Research and Extension Center, Virginia Polytechnic Institute and State University, Painter, VA 23420; hwilson@vt.edu
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P. Bernasconi , A. R. Woodworth , B. A. Rosen , M. V. Subramanian , and D. L. Siehl 1995. A naturally occurring point mutation confers broad range tolerance to herbicides that target acetolactate synthase. J. Biol. Chem. 270:1738117385.

R. S. Chaleff and C. J. Mauvais 1984. Acetolactate synthase is the site of action of two sulfonylurea herbicides in higher plants. Science. 224:14431445.

A. K. Chang and R. G. Duggleby 1998. Herbicide-resistant forms of Arabidopsis thaliana acetohydroxyacid synthase: characterization of the catalytic properties and sensitivity to inhibitors of four defined mutants. Biochem. J. 333:765777.

J. T. Christopher , S. B. Powles , and J. A. M. Holtum 1992. Resistance to acetolactate synthase-inhibiting herbicides in annual ryegrass (Lolium rigidum) involves at least two mechanisms. Plant Physiol. 100:19091913.

J. Durner , V. Gailus , and P. Boger 1990. New aspects on inhibition of plant acetolactate synthase by chlorsulfuron and imazaquin. Plant Physiol. 95:11441149.

B. C. Gerwick , M. V. Subramanian , and V. I. Loney-Gallant 1990. Mechanism of action of the 1,2,4-triazolo[1,5-a]pyrimidines. Pestic. Sci. 29:357364.

G. Mourad and J. King 1992. Effect of four classes of herbicides on growth and acetolactate-synthase activity in several variants of Arabidopsis thaliana . Planta. 188:491497.

D. H. Poston , H. P. Wilson , and T. E. Hines 2000. Imidazolinone resistance in several Amaranthus hybridus populations. Weed Sci. 48:508513.

D. H. Poston , J. Wu , K. K. Hatzios , and H. P. Wilson 2001. Enhanced sensitivity to cloransulam-methyl in imidazolinone-resistant smooth pigweed. Weed Sci. 49:711716.

K. Sathasivan , G. W. Haughn , and N. Murai 1990. Nucleotide sequence of a mutant acetolactate synthase gene from an imidazolinone-resistant Arabidopsis thaliana var. Columbia . Nucleic Acids Res. 18:2188.

K. Sathasivan , G. W. Haughn , and N. Murai 1991. Molecular basis of imidazolinone herbicide resistance in Arabidopsis thaliana var Columbia. Plant Physiol. 97:10441050.

D. L. Shaner 1999. Resistance to acetolactate synthase (ALS) inhibitors in the United States: history, occurrence, detection and management. J. Weed Sci. Tech. 44:405411.

D. L. Shaner , P. C. Anderson , and M. A. Stidham 1984. Imidazolinones: Potential inhibitors of acetohydroxyacid synthase. Plant Physiol. 76:545546.

L. J. Veldhuis , L. M. Hall , J. T. O'Donovan , W. Dyer , and J. C. Hall 2000. Metabolism-based resistance of a wild mustard (Sinapis arvensis L.) biotype to ethametsulfuron-methyl. J. Agric. Food Chem. 48:29862990.

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Weed Science
  • ISSN: 0043-1745
  • EISSN: 1550-2759
  • URL: /core/journals/weed-science
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