Skip to main content
×
×
Home

Distribution of Weedy Red Rice (Oryza sativa) Resistant to Imidazolinone Herbicides and its Relationship to Rice Cultivars and Wild Oryza Species

  • Ives C. G. R. Goulart (a1), Tereza C. O. Borba (a2), Valmir G. Menezes (a3) and Aldo Merotto (a1)
Abstract

Several weedy red rice populations have evolved resistance to imidazolinone herbicides worldwide. The understanding of the factors related to the herbicide resistance in weedy red rice is important to prevent its occurrence in new areas where imidazolinone-resistant rice cultivars are being used, and to manage the new rice cultivars resistant to herbicides with modes of action other than the acetolactate synthase (ALS)-inhibitors that are being developed. The objectives of this study were to analyze the relationship of weedy red rice populations from southern Brazil with rice cultivars and wild Oryza species and to evaluate the occurrence of introgression from rice cultivars and seed migration as the origin of resistance to imidazolinone herbicides in weedy rice. The study was based on 27 weedy red rice populations, seven rice cultivars, and four wild Oryza species that were genotyped with 24 simple sequence repeats and three ALS-specific single-nucleotide polymorphism markers. A large proportion of the genetic variation of the weedy red rice populations was found within (74%) rather than among populations (26%). The weedy red rice populations were more closely related to the newer rice cultivars that are imidazolinone-resistant than to the older cultivars. The South American native Oryza glumaepatula and the other wild Oryza species—Oryza rufipogon, Oryza longistaminata, and Oryza glaberrima—clustered separately from weedy red rice populations, indicating a low likelihood of introgression among weedy red rice and these wild species. Seed migration was an important factor in the genetic structure of the evaluated weedy red rice populations, although gene flow by pollen from resistant cultivars was the principal reason for the spread of herbicide resistance.

Copyright
Corresponding author
Corresponding author's E-mail: merotto@ufrgs.br
References
Hide All
Al-Ahmad, H, Gressel, J (2006) Mitigation using a tandem construct containing a selectively unfit gene precludes establishment ofBrassica napus transgenes in hybrids and backcrosses with weedy Brassica rapa . Plant Biotechnol J. 4:2333
Borba, TDO, Brondani, R, Rangel, P, Brondani, C (2009) Microsatellite marker-mediated analysis of the EMBRAPA rice core collection genetic diversity. Genetica. 137:293304
Botstein, D, White, RL, Skolnick, M, Davis, RW (1980) Construction of a genetic-linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet. 32:314331
Burgos, NR, Norsworthy, JK, Scott, RC, Smith, KL (2008) Red rice (Oryza sativa) status after 5 years of imidazolinone-resistant rice technology in Arkansas. Weed Technol. 22:200208
Busconi, M, Rossi, D, Lorenzoni, C, Baldi, G, Fogher, C (2012) Spread of herbicide-resistant weedy rice (red rice, Oryza sativa L.) after 5 years of Clearfield rice cultivation in Italy. Plant Biol. 14:751759
Cao, Q, Lu, BR, Xia, H, Rong, J, Sala, F, Spada, A, Grassi, F (2006) Genetic diversity and origin of weedy rice (Oryza sativa f. spontanea) populations found in north-eastern China revealed by simple sequence repeat (SSR) markers. Ann Bot. 98:12411252
Cao, Q-J, Xia, H, Yang, X, Lu, BR (2009) Performance of hybrids between weedy rice and insect-resistant transgenic rice under field experiments: implication for environmental biosafety assessment. J Integr Plant Biol. 51:11381148
Chung, JW, Park, YJ (2010) Population structure analysis reveals the maintenance of isolated sub-populations of weedy rice. Weed Res. 50:606620
Courtois, B, Froulin, J, Greco, R, Brusch, G, Droc, G, Hamelin, C, Ruiz, M, Clément, G, Evrard, JC, Coppenol, S, Katsantonis, D, Oliveira, M, Negrão, S, Matos, C, Cavigiolo, S, Lupotto, E, Piffanelli, Ahmadia, N (2012) Genetic diversity and population structure in a European collection of rice. Crop Sci. 52:16631675
Delouche, JC, Burgos, NR, Gealy, DR, Martin, GZS, Labrada, R, Larinde, M, Rosell, C (2007) Weedy Rices—Origin, Biology, Ecology, and Control. Rome Food and Agriculture Organization. 155 p
Doyle, JJ, Doyle, JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull. 19:1115
Ellstrand, NC (2009) Evolution of invasiveness in plants following hybridization. Biol Invasions. 11:10891091
Evanno, G, Regnaut, S, Goudet, J (2005) Detecting the number of clusters of individuals using the software structure: a simulation study. Mol Ecol. 14:26112620
Garris, AJ, Tai, TH, Coburn, J, Kresovich, S, McCouch, S (2005) Genetic structure and diversity in Oryza sativa L. Genetics. 169:16311638
Gealy, DR, Agrama, HA, Eizenga, GC (2009) Exploring genetic and spatial structure of US weedy red rice (Oryza sativa) in relation to rice relatives worldwide. Weed Sci. 57:627643
Gealy, DH, Agrama, H, Jia, MH (2012) Genetic analysis of a typical U.S. red rice phenotypes: indications of prior gene flow in rice fields? Weed Sci. 60:451461
Gealy, DR, Mitten, DH, Rutger, JN (2003) Gene flow between red rice (Oryza sativa) and herbicide-resistant rice (O. sativa): implications for weed management. Weed Technol. 17:627645
Goulart, ICGR, Matzenbacher, FO, Merotto, A Jr. (2012a) Differential germination pattern of rice cultivars resistant to imidazolinone herbicides carrying different acetolactate synthase gene mutations. Weed Res. 52:224232
Goulart, ICGR, Pacheco, M, Nunes, A, Merotto, A Jr. (2012b) Identification of origin and analysis of population structure of field-selected imidazolinone-herbicide resistant red rice. Euphytica. 187:437447
Gressel, J, Valverde, BE (2009) A strategy to provide long-term control of weedy rice while mitigating herbicide resistance transgene flow, and its potential use for other crops with related weeds. Pest Manag Sci. 65:723731
Hu, T, Qv, X, Xiao, G, Huang, X (2009) Enhanced tolerance to herbicide of rice plants by over-expression of a glutathione S-transferase. Mol Breed. 24:409418
Ishikawa, R, Toki, N, Imai, K, Sato, HI, Yamagishi, H, Shimamoto, Y, Ueno, K, Morishima, H, Sato, T (2005) Origin of weedy rice grown in Bhutan and the force of genetic diversity. Genet Resour Crop Evol. 52:395403
Jiang, ZX, Xia, HB, Basso, B, Lu, BR (2012) Introgression from cultivated rice influences genetic differentiation of weedy rice populations at a local spatial scale. Theor ApplGenet. 124:309322
Jung, HI, Kuk, YI, Back, K, Lee, DJ, Lee, S, Burgos, NR (2010) Resistance levels and fitness of protoporphyrinogen oxidase (PROTOX) inhibitor-resistant transgenic rice in paddy fields. Field Crops Res. 115:125131
Karasawa, MMG, Vencovsky, R, Silva, CM, Zucchi, MI, Oliveira, GCX, Veasey, EA (2007) Genetic structure of Brazilian wild rice (Oryza glumaepatula Steud., Poaceae) populations analyzed using microsatellite markers. Genet Mol Biol. 30:400410
Kawasaki, A, Imai, K, Ushiki, J, Ishii, T, Ishikawa, R (2009) Molecular constitution of weedy rice (Oryza sativa L.) found in Okayama prefecture, Japan. Breed Sci. 59:229236
Kuk, YI, Burgos, NR, Shivrain, VK (2008) Natural tolerance to imazethapyr in red rice (Oryza sativa). Weed Sci. 56:111
Lentini, Z, Espinoza, AM (2005) Coexistence of weedy rice and rice in tropical america—gene flow and genetic diversity. Pages 305322 in Gressel, J, ed. Crop Ferality and Volunteerism. Boca Raton, FL CRC
Lin, C, Fang, J, Xu, W, Zhao, T, Cheng, J, Tu, J, Ye, G, Shen, Z (2008) A built-in strategy for containment of transgenic plants: creation of selectively terminable transgenic rice. PLoS ONE. 3:e1818
Liu, K, Muse, SV (2005) PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics. 21:21282129
Livore, AB, Prina, AR, Birk, I, Singh, B, inventors; Instituto Nacional de Technologia Agropecuaria assignee (2005) March 10. Rice plants having increased tolerance to imidazolinone herbicides. U.S. patent 20070028318
Londo, JP, Schaal, BA (2007) Origins and population genetics of weedy red rice in the USA. Mol Ecol. 16:45234535
Mantel, N (1967) The detection of disease clustering and a generalized regression approach. Cancer Res. 27:209220
Marchezan, E, Menezes, NLD, Siqueira, CDA (2001) Controle da qualidade das sementes de arroz irrigado utilizadas em Santa Maria/RS. Ciência Rural. 31:375379
Menezes, VG, Mariot, CHP, Kalsing, A, Goulart, ICGR (2009) Arroz-vermelho (Oryza sativa) resistente aos herbicidas imidazolinonas. Planta Daninha. 27:10471052
Merotto, A Jr., Kalsing, A, Lopes, SIG (2013) Development of rice cultivars related to the technologies applied to the red rice control—public institutions—Brazil. CD. Proceedings of the II Latin-American Symposium of Red Rice. Porto Alegre, RS, Brazil Institute Riograndense of Rice and Federal University of Rio Grande do Sul
Michiels, F, Johnson, K, inventors; Plant Genetic Systems, N.V. (Gent, BE) assignee (2001) January 25. Glufosinate tolerant rice. U.S. patent 6333449
Nei, M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics. 89:583590
Nei, M, Tajima, F, Tateno, Y (1983) Accuracy of estimated phylogenetic trees from molecular data. J Mol Evol. 19:153170
Peakall, ROD, Smouse, PE (2006) Genalex 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes. 6:288295
Prathepha, P (2011) Microsatellite analysis of weedy rice (Oryza sativa f. spontanea) from Thailand and Lao PDR. Aust J Crop Sci. 5:4954
Pritchard, JK, Stephens, M, Donnelly, P (2000) Inference of population structure using multilocus genotype data. Genetics. 155:945959
Rajguru, SN, Burgos, NR, Shivrain, VK, McD Stewart, J (2005) Mutations in the red rice ALS gene associated with resistance to imazethapyr. Weed Sci. 53:946946
Reagon, M, Thurber, CS, Gros, BL, Olsen, KM, Jia, Y, Caicedo, AL (2010) Genomic patterns of nucleotide diversity in divergent populations of U.S. weedy rice. BMC Evol Biol. 10:180
Roso, AC, Merotto, A Jr., Delatorre, CA, Menezes, VG (2010) Regional scale distribution of imidazolinone herbicide-resistant alleles in red rice (Oryza sativa L.) determined through SNP markers. Field Crops Res. 119:175182
Scarabel, L, Cenghialta, C, Manuello, D, Sattin, M (2012) monitoring and management of imidazolinone-resistant red rice (Oryza sativa L., var. sylvatica) in Clearfield® Italian paddy rice. Agronomy. 2:371383
Shivrain, VK, Burgos, NR, Agrama, HA, Lawton-Rauh, A, Lu, BR, Sales, MA, Boyett, V, Gealy, DR, Moldenhauer, KAK (2010a) Genetic diversity of weedy red rice (Oryza sativa) in Arkansas, USA. Weed Res. 50:289302
Shivrain, VK, Burgos, NR, Sales, MA, Kuk, YI (2010b) Polymorphisms in the ALS gene of weedy rice (Oryza sativa L.) accessions with differential tolerance to imazethapyr. Crop Prot. 29:336341
Song, X, Liu, L, Wang, Z, Qiang, S (2009) Potential gene flow from transgenic rice (Oryza sativa L.) to different weedy rice (Oryza sativa f. spontanea) accessions based on reproductive compatibility. Pest Manag Sci. 65:862869
Song, Z, Lu, BR, Chen, J (2004) Pollen flow of cultivated rice measured under experimental conditions. Biodivers Conserv. 13:579590
Tamura, K, Dudley, J, Nei, M, Kumar, S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 24:15961599
Templeton, AR (2006) Gene flow and population subdivision. Pages 168203 in Templeton, AR. Population Genetics and Microevolutionary Theory. Hoboken, NJ J Wiley
Thurber, CS, Jia, MH, Jia, YL, Caicedo, AL (2013) Similar traits, different genes? Examining convergent evolution in related weedy rice populations. Mol Ecol. 22:685698
Wright, S (1949) The genetical structure of populations. Ann Eugenics. 15:323354
Xia, HB, Wang, W, Xia, H, Zhao, W, Lu, BR (2011) Conspecific crop–weed introgression influences evolution of weedy rice (Oryza sativa f. spontanea) across a geographical range. PLoS ONE. 6:e16189
Yoshida, H, Itoh, J, Ohmori, S, Miyoshi, K, Horigome, A, Uchida, E, Kimizu, M, Matsumura, Y, Kusaba, M, Satoh, H, Nagato, Y (2007) Superwoman1-cleistogamy, a hopeful allele for gene containment in GM rice. Plant Biotechnol J. 5:835846
Zhang, DL, Zhang, HL, Qi, YW, Wang, MX, Sun, JL, Ding, L, Li, ZC (2013) Genetic structure and eco-geographical differentiation of cultivated Hsien rice (Oryza sativa L. subsp. indica) in China revealed by microsatellites. Chin Sci Bull. 58:344352
Zhang, DL, Zhang, H, Wang, M, Sun, J, Qi, Y, Wang, F, Wei, X, Han, L, Wang, X, Li, Z (2009) Genetic structure and differentiation of Oryza sativa L. in China revealed by microsatellites. Theor Appl Genet. 119:11051117
Zhou, M, Xu, H, Wei, X, Ye, Z, Wei, L, Gong, W, Wang, Y, Zhu, Z (2006) Identification of a glyphosate-resistant mutant of rice 5-enolpyruvylshikimate 3-phosphate synthase using a directed evolution strategy. Plant Physiol. 140:184195
Ziska, LH, Gealy, DR, Tomecek, MB, Jackson, AK, Black, HL (2012) Recent and projected increases in atmospheric CO2 concentration can enhance gene flow between wild and genetically altered rice (Oryza sativa). PLoS ONE. 7(5):e37522
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Weed Science
  • ISSN: 0043-1745
  • EISSN: 1550-2759
  • URL: /core/journals/weed-science
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed