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Identification of compounds from Paris polyphylla (ChongLou) active against Dactylogyrus intermedius

Published online by Cambridge University Press:  03 April 2013

ZE-HONG LI ,
JIA-YU WAN ,
GUI-QIN WANG ,
FU-GUANG ZHAO  and
JI-HONG WEN
ZE-HONG LI
Affiliation:
College of Life Sciences, Jilin Agriculture University, Jilin, Changchun 130118, China
JIA-YU WAN
Affiliation:
Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130122, China
GUI-QIN WANG
Affiliation:
College of Life Sciences, Jilin Agriculture University, Jilin, Changchun 130118, China
FU-GUANG ZHAO
Affiliation:
College of Life Sciences, Jilin Agriculture University, Jilin, Changchun 130118, China
JI-HONG WEN*
Affiliation:
Obstetrics and Gynecology Department, The First Hospital of Jilin University, Changchun 130021, China
*
*Corresponding author. Obstetrics and Gynecology Department, The First Hospital of JiLin University, Changchun 130021, China. Fax: +86431-88782182. E-mail: jihong.wen@yahoo.com.cn
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Summary

The present study was designated to ascertain the anthelmintic activity of the rhizomes of Paris polyphylla and to isolate and characterize the active constituents. The methanol extract from rhizomes of P. polyphylla showed significant anthelmintic activity against Dactylogyrus intermedius with the median effective concentration (EC50) 22·5 mg L−1. Based on this finding, the methanol extract was fractionated by silica gel column chromatography in a bioassay-guided fractionation yielding 2 bioactive compounds, the structures of these compounds were elucidated as formosanin C and polyphyllin VII. The in vivo tests revealed that formosanin C and polyphyllin VII were significantly effective against D. intermedius with EC50 values of 0·6 and 1·2 mg L−1, respectively. The acute toxicities (LC50) of formosanin C and polyphyllin VII for grass carp were 2·8 and 2·9 mg L−1, respectively. The overall results provide important information for the potential application of formosanin C and polyphyllin VII in the therapy of serious infection caused by D. intermedius.

Keywords

Dactylogyrus intermediusParis polyphyllaformosanin Cpolyphyllin VIIanthelmintic
Type
Research Article
Information
Parasitology , Volume 140 , Issue 8 , July 2013 , pp. 952 - 958
Copyright
Copyright © Cambridge University Press 2013 

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