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Chapter 25 - Sperm Selection for ICSI by Morphology

MSOME/IMSI

from Section 4 - Insemination/ICSI

Published online by Cambridge University Press:  07 August 2023

By
Pierre Vanderzwalmen ,
Maximilian Schuff  and
Barbara Wirleitner
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

Spectacular progress in the therapeutic management of patients with severe male infertility was obtained with the implementation of intracytoplasmic sperm injection (ICSI) and has opened avenues to treating previously untreatable cases of infertility. However, it must be recognized that in the vast majority of ICSI cycles, the selection of the spermatozoon followed by the intracytoplasmic injection is done very quickly at low magnification and without detection possibilities for potential pathological morphological anomalies of spermatozoa. Strong correlations between sperm morphological characteristics and male fertility have been demonstrated. Thus, it seems reasonable to implement optimized non-invasive sperm selection techniques in ART based on morphological ’normalcy’ of spermatozoa. Almost 20 years ago, ’motile-sperm organelle morphology examination’ (MSOME) was introduced by Bartoov et al. Using Nomarski differential interference contrast optics (DIC) an improved three-dimensional view of the head and midpiece became available. This chapter will describe different aspects of sperm selection by morphology.

Keywords

morphologyMSOMEIMSIspermfertilization
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 186 - 195
Publisher: Cambridge University Press
Print publication year: 2023

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References

Palermo, G., Joris, H., Devroey, P. and Van Steirteghem, A. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 1992; 340:17–18.CrossRefGoogle ScholarPubMed
Coutton, C., Escoffier, J., Martinez, G., Arnoult, C. and Ray, P. Teratozoospermia: spotlight on the main genetic actors in the human. Hum Reprod Update 2015; 21:455–85.CrossRefGoogle ScholarPubMed
Jenkins, T., Aston, K., Hotaling, J., et al. Teratozoospermia and asthenozoospermia are associated with specific epigenetic signatures. Andrology 2016; 4:843–9.CrossRefGoogle ScholarPubMed
Vanderzwalmen, P., Bach, M., Gaspard, O., et al. Motile-sperm organelle-morphology examination and intracytoplasmic morphologically selected sperm injection: clinical and technical aspects, in A Practical Guide to Selecting Gametes and Embryos, ed. Montag, M., pp. 59–80 (Boca Raton, FL: CRC Press, 2014).Google Scholar
Bartoov, B., Berkovitz, A. and Eltes, F. Selection of spermatozoa with normal nuclei to improve the pregnancy rate with intracytoplasmic sperm injection. N Engl J Med 2001; 345:1067–8.CrossRefGoogle ScholarPubMed
Boitrelle, F., Albert, M., Petit, J.-M., et al. Small human sperm vacuoles observed under high magnification are pocket-like nuclear concavities linked to chromatin condensation failure. Reprod Biomed Online 2013; 27:201–11.CrossRefGoogle ScholarPubMed
Knez, K., Zorn, B., Tomazevic, T., Vrtacnik–Bokal, E. and Virant-Klun, I. The IMSI procedure improves poor embryo development in the same infertile couples with poor semen quality: A comparative prospective randomized study. Reprod Biol Endocrinol 2011; 9:123–30.CrossRefGoogle ScholarPubMed
Vanderzwalmen, P., Hiemer, A., Rubner, P., et al. Blastocyst development after sperm selection at high magnification is associated with size and number of nuclear vacuoles. Reprod Biomed Online 2008; 17:617–27.CrossRefGoogle ScholarPubMed
Yurchuk, T., Petrushkо, M., Gapon, A. , Piniaiev, V. and Kuleshova, L. The impact of cryopreservation on the morphology of spermatozoa in men with oligoasthenoteratozoospermia. Cryobiology 2021; 100:117–24.CrossRefGoogle ScholarPubMed
Vaughan, D., Leung, A., Resetkova, N., et al. How many oocytes are optimal to achieve multiple live births with one stimulation cycle? The one-and-done approach. Fertil Steril 2017; 107:397–404.CrossRefGoogle ScholarPubMed
Setti, A., Braga, D., Provenza, R., Iaconelli, A. and Borges, E. Oocyte ability to repair sperm DNA fragmentation: the impact of maternal age on intracytoplasmic sperm injection outcomes. Fertil Steril 2021; 116(1):123–9.CrossRefGoogle ScholarPubMed
Berkovitz, A., Dekel, Y., Goldstein, R., et al. The significance of human spermatozoa vacuoles can be eleucidated by a novel procedure of array comparative genomic hybridization. Hum Reprod 2018; 3:563–71.Google Scholar
Neyer, A., Zintz, M., Stecher, A., et al. The impact of paternal factors on cleavage stage and blastocyst development analyzed by time-lapse imaging: a retrospective observational study. J Assist Reprod Genet 2015; 32:1607–14.CrossRefGoogle ScholarPubMed

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