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Microsporidian diversity in the aquatic isopod Asellus aquaticus
- Daniel Grabner, Annemie Doliwa, Lidia Sworobowicz, Anna Wysocka, Alexander Weigand, Michał Grabowski, Tomasz Mamos, Bernd Sures
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- Journal:
- Parasitology / Volume 149 / Issue 13 / November 2022
- Published online by Cambridge University Press:
- 25 August 2022, pp. 1729-1736
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- Article
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We conducted a molecular survey on microsporidian diversity in different lineages (operational taxonomic units = OTUs) of Asellus aquaticus from 30 sites throughout Europe. Host body length was determined, and DNA was extracted from host tissue excluding the intestine and amplified by microsporidian-specific primers. In total, 247 A. aquaticus specimens were analysed from which 26.7% were PCR-positive for microsporidians, with significantly more infections in larger individuals. Prevalence ranged between 10 and 90%. At 9 sites, no microsporidians were detected. A significant relationship was found between the frequency of infected individuals and habitat type, as well as host OTU. The lowest proportion of infected individuals was detected in spring-habitats (8.7%, n = 46) and the highest in ponds (37.7%, n = 53). Proportion of infected individuals among host OTUs A, D and J was 31.7, 21.7 and 32.1%, respectively. No infections were detected in OTU F. Our results are, however, accompanied by a partially low sample size, as only a minimum of 5 individuals was available at a few locations. Overall, 17 different microsporidian molecular taxonomic units (MICMOTUs) were distinguished with 5 abundant isolates (found in 4–17 host individuals) while the remaining 12 MICMOTUs were “rare” and found only in 1–3 host individuals. No obvious spatio-genetic pattern could be observed. The MICMOTUs predominantly belonged to Nosematida and Enterocytozoonida. The present study shows that microsporidians in A. aquaticus are abundant and diverse but do not show obvious patterns related to host genetic lineages or geography.
Eu Succession Regulation: Choice of Applicable Law and Protection of Family Members
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- By Anna Wysocka
- Edited by Katharina Boele-Woelki, Joanna K. Miles, Jens M. Scherpe
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- Book:
- The Future of Family Property in Europe
- Published by:
- Intersentia
- Published online:
- 08 May 2020
- Print publication:
- 22 April 2011, pp 383-406
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Summary
INTRODUCTION
On 14 October 2009, the European Commission published the eagerly awaited proposal for a European Union Regulation on jurisdiction, applicable law, recognition and enforcement of decisions and authentic instruments in matters of succession and the creation of a European Certificate of Succession1 (hereinafter referred to as the “proposal” or “Succession Regulation”).
The preparation of this proposal was preceded by a comparative research into the international succession laws of the Member States conducted by German Notarial Institute located in Wurzburg (Deutsches Notarinstitut). In 2002, on the basis of national reports submitted by experts from 15 old Member States, the German Notarial Institute prepared a summary report which contained a synthesis of the private international laws of succession in the European Union, as well as recommendations as to the future unification of international succession within the European Union (hereinafter referred to as the “DNotI report”). The results of the comparative study and recommendations as to the future unification of rules on jurisdiction, applicable law and the recognition of judgments in succession matters were discussed during the conference held in Brussels on May 10–11, 2004. Additionally, it was proposed that the future EU instrument could influence the creation of a European certificate of succession and a European register of wills.
The next milestone on the road towards a unified European private international law of succession was the EU Council's summit held in Brussels on November 4–5, 2004, during which the document entitled “The Hague Programme: Strengthening freedom, security and justice in the European Union” (hereinafter: “the Hague Programme”) was adopted. The Hague Programme assumed that until 2011 the law applicable to contractual obligations, non-contractual obligations, maintenance, matrimonial property regimes, divorces and succession will be regulated at the EU level. As underlined in the Hague Programme, EU instruments should cover only matters of private international law and should not be based on harmonized concepts of the family or marriage. In the Hague Programme the EU Council invited the European Commission to submit, in 2005, “a green paper on the conflict of laws in matters of succession, including the question of jurisdiction, mutual recognition and enforcement of decisions in this area, a European certificate of inheritance and a mechanism allowing precise knowledge of the existence of last wills and testaments of residents of the European Union”. In response to this invitation, on March 1, 2005 the EU Commission presented a document called the “Green Paper: Successions and wills” (hereinafter: “Green Paper”).