Hunter-gatherer occupations of the forests of Patagonia are still not well understood compared to those of the open steppe and the coasts. The paucity of forest sites with a thorough chronological framework and the incomplete picture of climate-human dynamics in such environments pose a challenge to understanding the occupations of such habitats. This paper presents recent work at the Las Quemas rockshelter, an archaeological site located in the Andean forests of the Aisén region (44°38’ S) dating between 6100 and 2400 cal B.P. We examine the context of the site, its rock art, and the technological and functional dimensions of its lithic assemblage. By comparing these records with local environmental reconstructions that report forest area fluctuations after the Post-Glacial period, this study provides novel data for a comprehensive regional-scale model of human occupations in central Patagonian forests. Following an initial mid-Holocene occupation, recurring occupational events at the site coincide with a significant contraction of forested area in the region during the late Holocene. The results are discussed in regard to regional archaeological data and current models of forest occupation in Patagonia.