Carrot is a high-value cash crop that is grown in Israel throughout theyear. Egyptian broomrape is a chlorophyll-lacking, obligate, rootholoparasite that parasitizes members of many botanical families, includingthe Apiaceae. At high infestation levels, Egyptian broomrape can cause totalyield loss in carrot. A protocol has been developed for the control ofEgyptian broomrape in carrot. Because carrots are grown in Israel underfall, winter, and spring conditions, information about the relations betweenthe efficacy of control and temperature is important. Therefore, theobjective of this study was to investigate the response of carrot andEgyptian broomrape to herbicides at different phenological stages undervarying temperature regimes. This study was conducted undertemperature-controlled conditions in a multiclimate greenhouse and in a nethouse. Applications of the imidazolinone herbicides imazapic and imazamox(each applied at 4.8 g ai ha−1) injured carrot plants and reducedyield and yield quality. Glyphosate effectively controlled Egyptianbroomrape and did not negatively affect the carrot plants when applied threetimes at ≤ 108 g ae ha−1. High temperatures increased the carrotplants’ sensitivity to glyphosate. This study found that three applicationsof glyphosate at 108 g ae ha−1 can prevent Egyptian broomrapedamage without causing any damage to the carrot crop. Our results indicatethat weather conditions can affect herbicide phytotoxicity in carrot. Thehighest temperature at the time of herbicide application corresponded to thestrongest observed phytotoxic effect. To summarize, effective Egyptianbroomrape control can be achieved by three sequential foliar applications ofglyphosate (108 g ae ha−1), beginning during the early parasitismstage (i.e., small tubercles). Moreover, applying glyphosate on carrot athigh temperature (i.e., 28/22 C day/night temperatures) can injure carrotplants and reduce control efficacy.