Skip to main content Accessibility help
×
×
Home

Dietary n-6:n-3 fatty acid ratio in the perinatal period affects bone parameters in adult female rats

  • M. Korotkova (a1), C. Ohlsson (a2), L. Å. Hanson (a3) and B. Strandvik (a1)

Abstract

PUFA and their metabolites are important regulators of bone formation and resorption. The effect of PUFA on bone growth may be especially striking during the perinatal period. The aim of the present study was to investigate the effect of diets with different n-6:n-3 fatty acid (FA) ratios during the perinatal period on bone parameters in the adult offspring. During late gestation and throughout lactation, rat dams were fed an isoenergetic diet containing 70 g linseed oil (n-3 diet), soyabean oil (n-6+n-3 diet) or sunflower-seed oil (n-6 diet) per kg with n-6:n-3 FA ratios of 0·4, 9 and 216, respectively. The offspring were weaned onto an ordinary chow and followed until 30 weeks of age. Bone parameters were analysed using peripheral quantitative computerised tomography and dual-energy X-ray absorptiometry. Femur length and cortical cross-sectional bone area and bone mineral content were significantly higher in the n-6+n-3 group than in the other groups. Cortical bone thickness in the n-6+n-3 group was increased compared with the n-3 group, but most cortical bone parameters did not differ between the n-3 and n-6 groups. The results suggest that regulatory mechanisms were influenced by the n-6:n-3 FA ratio early in life and not compensated for by the introduction of an ordinary diet after weaning.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Dietary n-6:n-3 fatty acid ratio in the perinatal period affects bone parameters in adult female rats
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Dietary n-6:n-3 fatty acid ratio in the perinatal period affects bone parameters in adult female rats
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Dietary n-6:n-3 fatty acid ratio in the perinatal period affects bone parameters in adult female rats
      Available formats
      ×

Copyright

Corresponding author

*Corresponding author: Dr Marina Korotkova, fax +46 31 21 70 23, email Marina.Korotkova@cmm.ki.se

References

Hide All
Chlebna-Sokol, D & Rusinska, A (2001) Serum insulin/like growth factor I, bone mineral density and biochemical markers of bone metabolism in children with idiopathic osteoporosis. Endocr Regul 35, 201208.
Folch, J, Lees, M, Sloane-Stanley, GH (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226, 497509.
Gordeladze, JO, Drevon, CA, Syversen, U & Reseland, JE (2002) Leptin stimulates human osteoblastic cell proliferation, de novo collagen synthesis, and mineralization: impact on differentiation markers, apoptosis, and osteoclastic signaling. J Cell Biochem 85, 825836.
Innis, SM & Kuhnlein, HV (1988) Long-chain n -3 fatty acids in breast milk of Inuit women consuming traditional foods. Early Hum Dev 18, 185189.
Iwami-Morimoto, Y, Yamaguchi, K & Tanne, K (1999) Influence of dietary n -3 polyunsaturated fatty acid on experimental tooth movement in rats. Angle Orthod 69, 365371.
Jones, G, Riley, MD & Dwyer, T (2000) Maternal diet during pregnancy is associated with bone mineral density in children: a longitudinal study. Eur J Clin Nutr 54, 749756.
Judex, S, Wohl, GR, Wolff, RB, Leng, W, Gillis, AM & Zernicke, RF (2000) Dietary fish oil supplementation adversely affects cortical bone morphology and biomechanics in growing rabbits. Calcif Tissue Int 66, 443448.
Knapp, HR (1990) Prostaglandins in human semen during fish oil ingestion: evidence for in vivo cyclooxygenase inhibition and appearance of novel trienoic compounds. Prostaglandins 39, 407423.
Kokkinos, PP, Shaye, R, Alam, BS & Alam, SQ (1993) Dietary lipids, prostaglandin E2 levels, and tooth movement in alveolar bone of rats. Calcif Tissue Int 53, 333337.
Korotkova, M, Gabrielsson, B, Hanson, LA & Strandvik, B (2001) Maternal essential fatty acid deficiency depresses serum leptin levels in suckling rat pups. J Lipid Res 42, 359365.
Korotkova, M, Gabrielsson, B, Lonn, M, Hanson, LA & Strandvik, B (2002) Leptin levels in rat offspring are modified by the ratio of linoleic to alpha-linolenic acid in the maternal diet. J Lipid Res 43, 17431749.
Li, Y, Seifert, MF, Ney, DM, Grahn, M, Grant, AL, Allen, KG & Watkins, BA (1999) Dietary conjugated linoleic acids alter serum IGF-I and IGF binding protein concentrations and reduce bone formation in rats fed ( n -6) or ( n -3) fatty acids. J Bone Miner Res 14, 11531162.
Lucas, A (1991) Programming by early nutrition in man. Ciba Found Symp 156, 3850.
Lucas, A (1998) Programming by early nutrition: an experimental approach. J Nutr 128, 401S406S.
McCarthy, TL, Centrella, M, Raisz, LG & Canalis, E (1991) Prostaglandin E2 stimulates insulin-like growth factor I synthesis in osteoblast-enriched cultures from fetal rat bone. Endocrinology 128, 28952900.
Mehta, G, Roach, HI, Langley-Evans, S, Taylor, P, Reading, I, Oreffo, RO, Aihie-Sayer, A, Clarke, NM & Cooper, C (2002) Intrauterine exposure to a maternal low protein diet reduces adult bone mass and alters growth plate morphology in rats. Calcif Tissue Int 71, 493498.
Moreira-Andres, MN, Papapietro, K, Canizo, FJ, Rejas, J, Larrodera, L & Hawkins, FG (1995) Correlations between bone mineral density, insulin-like growth factor I and auxological variables. Eur J Endocrinol 132, 573579.
Ohlsson, C, Bengtsson, BA, Isaksson, OG, Andreassen, TT & Slootweg, MC (1998) Growth hormone and bone. Endocr Rev 19, 5579.
Okada, Y, Lorenzo, JA, Freeman, AM, Tomita, M, Morham, SG, Raisz, LG & Pilbeam, CC (2000) Prostaglandin G/H synthase-2 is required for maximal formation of osteoclast-like cells in culture. J Clin Invest 105, 823832.
Raisz, LG & Fall, PM (1990) Biphasic effects of prostaglandin E2 on bone formation in cultured fetal rat calvariae: interaction with cortisol. Endocrinology 126, 16541659.
Rocquelin, G, Kiffer, J, Tapsoba, S & Bouda, C (2001) High proportions of n -6 polyunsaturated fatty acids in mature milk of mothers in Ouagadougou, Burkina Faso. Acta Paediatr 90, 450452.
Sakaguchi, K, Morita, I & Murota, S (1994) Eicosapentaenoic acid inhibits bone loss due to ovariectomy in rats. Prostaglandins Leukot Essent Fatty Acids 50, 8184.
Sjogren, K, Sheng, M, Moverare, S, Liu, JL, Wallenius, K, Tornell, J, Isaksson, O, Jansson, JO, Mohan, S & Ohlsson, C (2002) Effects of liver-derived insulin-like growth factor I on bone metabolism in mice. J Bone Miner Res 17, 19771987.
Steppan, CM, Crawford, DT, Chidsey-Frink, KL, Ke, H & Swick, AG (2000) Leptin is a potent stimulator of bone growth in ob/ob mice. Regul Pept 92, 7378.
Takiguchi, T, Kobayashi, M, Nagashima, C, Yamaguchi, A, Nishihara, T & Hasegawa, K (1999) Effect of prostaglandin E2 on recombinant human bone morphogenetic protein-2-stimulated osteoblastic differentiation in human periodontal ligament cells. J Periodontal Res 34, 431436.
Vidal, O, Lindberg, MK, Hollberg, K, Baylink, DJ, Andersson, G, Lubahn, DB, Mohan, S, Gustafsson, JA & Ohlsson, C (2000) Estrogen receptor specificity in the regulation of skeletal growth and maturation in male mice. Proc Natl Acad Sci USA 97, 54745479.
Watkins, BA, Li, Y, Allen, KG, Hoffmann, WE & Seifert, MF (2000) Dietary ratio of ( n -6)/( n -3) polyunsaturated fatty acids alters the fatty acid composition of bone compartments and biomarkers of bone formation in rats. J Nutr 130, 22742284.
Watkins, BA, Li, Y, Lippman, HE & Seifert, MF (2001) Omega-3 polyunsaturated fatty acids and skeletal health. Exp Biol Med (Maywood) 226, 485497.
Watkins, BA, Shen, CL, Allen, KG & Seifert, MF (1996) Dietary ( n -3) and ( n -6) polyunsaturates and acetylsalicylic acid alter ex vivo PGE2 biosynthesis, tissue IGF-I levels, and bone morphometry in chicks. J Bone Miner Res 11, 13211332.
Watkins, BA, Shen, CL, McMurtry, JP, Xu, H, Bain, SD, Allen, KG & Seifert, MF (1997) Dietary lipids modulate bone prostaglandin E2 production, insulin-like growth factor-I concentration and formation rate in chicks. J Nutr 127, 10841091.
Windahl, SH, Vidal, O, Andersson, G, Gustafsson, JA & Ohlsson, C (1999) Increased cortical bone mineral content but unchanged trabecular bone mineral density in female ERbeta(-/-) mice. J Clin Invest 104, 895901.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed