In recent field studies, suspected gymnophallid metacercariae were histologically located in the mantle of mussels from the Norwegian Sea. Mussels from the sites in which that infection was detected also presented abnormally high pearl numbers. It has been previously described that gymnophallid metacercariae could cause pearl formation processes in mussels, as a host reaction to encapsulate these metacercariae. Given the pathological host reaction these parasites elicit, a study was performed to identify gymnophallid metacercariae found in mussels collected from Tromsø at morphological and molecular level and to assess, by the use of molecular tools, the relationship between the parasite and the biological material inside the pearls. As a result, Gymnophallus bursicola metacercariae infecting Norwegian Mytilus edulis were identified according to morphological characters, along with the first 18S rDNA and COI sequences for this trematode species. In addition, parasite DNA from the core of the pearls was extracted and amplified for the first time, confirming the parasitological origin of these pearls. This procedure could allow identifying different parasitic organisms responsible for the generation of pearls in bivalves.

Direct numerical simulations of a temporally developing, low-speed, variable-density, turbulent, plane mixing layer are performed. The Navier–Stokes equations in the low-Mach-number approximation are solved using a novel algorithm based on an extended version of the velocity–vorticity formulation used by Kim et al. (J. Fluid Mech., vol 177, 1987, 133–166) for incompressible flows. Four cases with density ratios $s=1,2,4$ and 8 are considered. The simulations are run with a Prandtl number of 0.7, and achieve a $Re_{\unicode[STIX]{x1D706}}$ up to 150 during the self-similar evolution of the mixing layer. It is found that the growth rate of the mixing layer decreases with increasing density ratio, in agreement with theoretical models of this phenomenon. Comparison with high-speed data shows that the reduction of the growth rates with increasing density ratio has a weak dependence with the Mach number. In addition, the shifting of the mixing layer to the low-density stream has been characterized by analysing one-point statistics within the self-similar interval. This shifting has been quantified, and related to the growth rate of the mixing layer under the assumption that the shape of the mean velocity and density profiles do not change with the density ratio. This leads to a predictive model for the reduction of the growth rate of the momentum thickness, which agrees reasonably well with the available data. Finally, the effect of the density ratio on the turbulent structure has been analysed using flow visualizations and spectra. It is found that with increasing density ratio the longest scales in the high-density side are gradually inhibited. A gradual reduction of the energy in small scales with increasing density ratio is also observed.

The genus Perkinsus includes protistan parasites infecting marine molluscs throughout the world, some of which are associated with mass mortalities. Life cycle involves vegetative proliferation within the host, by which a cell named trophozoite undergoes successive bipartitioning. Other stages have been observed in vitro or in vivo, depending on the species: hypnospore, zoosporangium and zoospore. Molecular taxonomy supports a close affinity between dinoflagellates and Perkinsus spp. Six species of Perkinsus are currently considered valid: P. marinus, P. olseni, P. qugwadi, P. chesapeaki, P. andrewsi and P. mediterraneus. Histology and, above all, incubation of host tissues in Ray's fluid thioglycollate medium (RFTM) are classic diagnostic methods. In addition, more sensitive and quicker molecular diagnostic techniques based on either immunoassays or PCR have been developed for Perkinsus spp. Epizootiological studies have shown a marked influence of water temperature and salinity on P. marinus infection in oysters Crassostrea virginica, thus determining parasite geographical range and temporal disease dynamics (seasonality). In vitro cultures have been established for four Perkinsus spp. Immune response to infection varies depending on host and involves phagocytosis or encapsulation of the parasite cells by host haemocytes. A polypeptide is secreted by clam Tapes philippinarum haemocytes that could kill the parasite. In vitro cultured P. marinus cells secrete proteases that are likely involved in degradation of host tissues. P. marinus can suppress the toxic oxygen radicals produced by host haemocytes. In addition to host death, sublethal effects caused by Perkinsus spp. (reduction of fecundity, growth, and condition) may have significant ecological and economic implications. Various strategies have been assayed to mitigate the consequences of P. marinus epizootics on the oyster industry: modifications of management/culture procedures, selective breeding to obtain resistant oyster strains, and the use of triploid oysters and allochthonous oyster species. Some chemotherapeutants have been proved to inhibit or kill parasite cells in vitro.