The effects of thirteen plant growth regulators and herbicides on glucose absorption, metabolism, and incorporation in pea root tissues were studied. Both indoleacetic acid (IAA) and gibberellic acid (GA) caused no effects, while α-naphthalene-acetic acid (α-NAA) and β-naphthaleneacetic acid (β-NAA) caused an increase in catabolic and a decrease in anabolic processes, with the C-1 carbon undergoing the largest change. The absorption of glucose was also inhibited considerably by the synthetic auxins. The carbamates, isopropyl N-phenylcarbamate (IPC), n-Ethyl-N,N-di-n-propylthiolcarbamate (EPTC), and 3-(p-chlorophenyl)-1,1-dimethylurea (monuron) inhibited slightly the glucose absorption. All affected the catabolic process to a certain extent while only IPC caused a change in the anabolic process. Both 2,3,6-trichlorobenzoic acid (TBA) and 2,3,5-triiodobenzoic acid (TIBA) decreased glucose absorption and increased glucose-1-C14 catabolism. TIBA also affected the anabolic process. Other herbicides, 3-amino-1,2,4-triazole (amitrole), maleic hydrazide (MH), 2-chloro-4,6-bis(ethylamino)-s-triazine (simazine), and 2,2-dichloropropionic acid (dalapon) caused varied degrees of inhibition to glucose absorption. Both simazine and dalapon increased C-1 catabolism and exerted no effect on the anabolic process. Only dalapon altered the catabolism of glucose-6-C14. Amitrole and MH decreased the over-all catabolic process with no effect on the pathway; both chemicals increased the anabolic process.