Cereal rye (Secale cereale L.) cover crop and preemergence herbicides are important components of an integrated weed management program for waterhemp [Amaranthus tuberculatus (Moq.) Sauer] and Palmer amaranth (Amaranthus palmeri S. Watson) management in soybean [Glycine max (L.) Merr.]. Accumulating adequate cereal rye biomass for effective suppression of Amaranthus spp. can be challenging in the upper Midwest due to the short window for cereal rye growth in a corn–soybean rotation. Farmers are adopting the planting green system to optimize cereal rye biomass production and weed suppression. This study aimed to evaluate the feasibility of planting soybean green when integrated with preemergence herbicides for the control of Amaranthus spp. under two soybean planting time frames. The study was conducted across 19 site-years in the United States over the 2021 and 2022 growing seasons. Factors included cover crop management practices (“no-till,” “cereal rye early-term,” and “cereal rye plant-green”), soybean planting times (“early” and “late”), and use of preemergence herbicides (“NO PRE” and “YES PRE”). Planting soybean green increased cereal rye biomass production by 33% compared with early termination. Greater cereal rye biomass production when planting green provided a 44% reduction in Amaranthus spp. density compared with no-till. The use of preemergence herbicides also resulted in a 68% reduction in Amaranthus spp. density compared with NO PRE. Greater cereal rye biomass produced when planting green reduced soybean stand, which directly reduced soybean yield in some site-years. Planting soybean green is a feasible management practice to optimize cereal rye biomass production, which, combined with preemergence herbicides, provided effective Amaranthus spp. management. Soybean stand was a key factor in maintaining soybean yields compared with no-till when planting green. Farmers should follow best management recommendations for proper planter and equipment setup to ensure effective soybean establishment under high levels of cereal rye biomass when planting green.

Drill-interseeding cover crops into corn (Zea mays L.) is an emerging establishment method in northern U.S. production regions. However, cover crop performance in interseeded systems remains variable, and creating environments that are conducive to cover crop but not weed growth is challenging. Cultural practices that partition resources between corn and interseeded cover crops have potential to improve performance if weeds are adequately managed. This study evaluated interactions among corn hybrids differing in leaf architecture (upright, pendulum), corn row spacing (76 cm, 152 cm), and interseeding timing (V3, V6) on light transmittance, relative fitness of cover crop species (cereal rye [Secale cereale L.], annual ryegrass (Lolium multiflorum Lam), red clover [Trifolium pratense L.]) and weeds, and corn grain yield at three U.S. Northeast locations. Results showed that light transmittance through the corn canopy was greater in 152-cm row spacing compared with 76-cm row spacing at the V6 growth stage, with the magnitude of difference increasing at the V10 corn growth stage. Corn hybrids had a marginal effect on light transmittance. The effect of row spacing and interseeding timing on fall cover crop biomass varied across cover crop species and locations. In 76-cm rows, interseeding earlier (V3) increased cover crop biomass production. The relative fitness of cover crops was greater than that of weeds in each combination of cultural practices that included narrow spacing (76 cm), whereas the relative fitness of weeds was greater than that of cover crops when interseeding in wide rows (152 cm). The effect of row spacing on corn yield varied among locations, with higher yields observed in 76-cm row spacing compared with 152-cm at two of three locations. Our results show that interseeding early (V3) on 76-cm row spacing can balance cover crop and corn production management goals, while placing cover crops at a relative fitness advantage over weeds.

Preemergence herbicides associated with cereal rye (Secale cereale L.) cover crop (hereafter “cereal rye”) can be an effective waterhemp [Amaranthus tuberculatus (Moq.) Sauer.] and Palmer amaranth (Amaranthus palmeri S. Watson) management strategy in soybean [Glycine max (L.) Merr.] production. Delaying cereal rye termination until soybean planting (planting green) optimizes biomass production and weed suppression but might further impact the fate of preemergence herbicides. Limited research is available on the fate of preemergence herbicides applied over living cereal rye in the planting green system. Field experiments were conducted in Illinois, Kansas, Pennsylvania, and Wisconsin to evaluate the fate of flumioxazin and pyroxasulfone and Amaranthus spp. residual control under different cover crop management practices in soybean in 2021 and 2022 (8 site-years). A flumioxazin + pyroxasulfone herbicide premix was applied preemergence at soybean planting under no-till without cereal rye, cereal rye early terminated before soybean planting, and cereal rye terminated at soybean planting. Flumioxazin and pyroxasulfone concentrations in the soil were quantified at 0, 7, and 21 d after treatment (DAT), and Amaranthus spp. density was determined at postemergence herbicide application. The presence of cereal rye biomass intercepted flumioxazin and pyroxasulfone at preemergence application and reduced concentration in the soil when compared with no-till, mainly at 0 DAT. Main differences in herbicide concentration were observed between no-till and cereal rye treatments rather than cereal rye termination times. Despite reducing herbicide concentration in the soil, the presence of the cereal rye biomass did not affect early-season residual Amaranthus spp. control. The adoption of effective preemergence herbicides associated with a properly managed cereal rye cover crop is an effective option for integrated Amaranthus spp. management programs in soybean production systems.

Delaying cover crop termination until cash crop planting (i.e., planting green) is an emerging no-till practice. Improved management recommendations are needed for optimizing weed suppression benefits while minimizing other pest, fertility, and crop management risks when planting green in corn production systems. In a 2-yr field experiment, we evaluated the interaction between cereal rye residue management tactics (standing residue, roll-crimping, roll-crimping with row cleaners) and herbicide programs (1-pass preemergence [PRE], 2-pass postemergence [POST]) when planting green on weed recruitment spatial patterns and corn performance compared to standard termination (14 d preplant [DPP]) and ryelage harvest (14 DPP) practices. In a 2-yr on-farm experiment, we evaluated corn performance in response to the same residue management tactics. Cereal rye biomass production varied significantly across years in on-station experiments, with average (4.9 Mg ha−1) and anomalous (9.9 Mg ha−1) levels observed in 2020 and 2021, respectively. In 2020, planting green with an integrated roll-crimper/row cleaner system resulted in greater intrarow weed density compared with planting green into standing cereal rye. Interrow weed density was lower when roll-crimping was employed compared to early termination (14 DPP). Planting green into standing cereal rye resulted in greater mean corn height (V5 stage) compared to other treatments, but corn population and yield did not differ. In 2021, few differences in weed recruitment patterns were observed, but corn population and yield were significantly lower in planting green treatments compared to early termination. In both years, late-season weed biomass was lower in two-pass POST programs compared to one-pass PRE programs. On-farm trials showed that planting green into standing residue increases corn height and can reduce corn populations, which may lead to reduce yields. Our results suggest that management recommendations for optimizing herbicide application timing should consider intrarow and interrow weed recruitment dynamics associated with residue management tactics needed to optimize corn performance.

Using declassified colonial and British records in Hong Kong and London, as well as memoirs of former leftists and newspapers, this article explores the strategies the Hong Kong colonial government employed in a propaganda campaign to garner political support of the rural population in the New Territories, a porous land frontier during the Cold War. It also analyses the varying political orientations of migrant farmers, who often had received economic benefits from both the colonial government and the leftist organizations. This article reveals that the colonial government established the Vegetable Marketing Organization (VMO), a state-owned enterprise, to first nationalize the vegetable wholesale market in the immediate post-war period, and subsequently used it to combat increasing political influence and anti-government activities of the communist-controlled Society of Plantations. Despite the improvement of the livelihood of immigrant farmers, the VMO Scheme failed to out-compete the Society economically, which was ultimately eliminated by draconian measures. Through studying the agrarian politics and economic contestations in Hong Kong’s rural area, this article provides a lens on how the Cold War was played out at a village level in East Asia.

OBJECTIVES/GOALS: Glioblastomas (GBMs) are heterogeneous, treatment-resistant tumors that are driven by populations of cancer stem cells (CSCs). In this study, we perform an epigenetic-focused functional genomics screen in GBM organoids and identify WDR5 as an essential epigenetic regulator in the SOX2-enriched, therapy resistant cancer stem cell niche. METHODS/STUDY POPULATION: Despite their importance for tumor growth, few molecular mechanisms critical for CSC population maintenance have been exploited for therapeutic development. We developed a spatially resolved loss-of-function screen in GBM patient-derived organoids to identify essential epigenetic regulators in the SOX2-enriched, therapy resistant niche. Our niche-specific screens identified WDR5, an H3K4 histone methyltransferase responsible for activating specific gene expression, as indispensable for GBM CSC growth and survival. RESULTS/ANTICIPATED RESULTS: In GBM CSC models, WDR5 inhibitors blocked WRAD complex assembly and reduced H3K4 trimethylation and expression of genes involved in CSC-relevant oncogenic pathways. H3K4me3 peaks lost with WDR5 inhibitor treatment occurred disproportionally on POU transcription factor motifs, required for stem cell maintenance and including the POU5F1(OCT4)::SOX2 motif. We incorporated a SOX2/OCT4 motif driven GFP reporter system into our CSC cell models and found that WDR5 inhibitor treatment resulted in dose-dependent silencing of stem cell reporter activity. Further, WDR5 inhibitor treatment altered the stem cell state, disrupting CSC in vitro growth and self-renewal as well as in vivo tumor growth. DISCUSSION/SIGNIFICANCE: Our results unveiled the role of WDR5 in maintaining the CSC state in GBM and provide a rationale for therapeutic development of WDR5 inhibitors for GBM and other advanced cancers. This conceptual and experimental framework can be applied to many cancers, and can unmask unique microenvironmental biology and rationally designed combination therapies.