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Glufosinate Does Not Affect Floral Morphology and Pollen Viability in Glufosinate-Resistant Cotton
- Walter E. Thomas, Wendy A. Pline, John W. Wilcut, Keith L. Edmisten, Randy Wells, Ryan P. Viator, Mary D. Paulsgrove
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- Journal:
- Weed Technology / Volume 18 / Issue 2 / June 2004
- Published online by Cambridge University Press:
- 20 January 2017, pp. 258-262
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Studies were conducted to determine whether glufosinate treatments to glufosinate-resistant cotton caused changes in floral morphology, pollen viability, and seed set. Four glufosinate treatments were included: (1) glufosinate applied postemergence over the top (POST) at the four-leaf stage, (2) glufosinate applied POST at the eight-leaf stage, (3) the first two treatments sequentially, and (4) a POST application at the four-leaf stage followed by (fb) a postemergence-directed stem application (PDS) at the eight-leaf stage. Glufosinate was consistently applied at 0.49 kg ai/ha. A nontreated control was included. Glufosinate treatments did not affect stigma height, length of the staminal column, or pollen viability. However, the distance from the top anther to the tip of the stigma was less in plants treated with an eight-leaf POST treatment than in nontreated plants, although this difference is not likely to influence pollen deposition because in both cases anthers reached above the stigma tip. Plants receiving four-leaf POST fb eight-leaf PDS treatment with glufosinate had eight seeds per boll less than nontreated plants; however, the more rigorous four-leaf POST fb eight-leaf POST treatment did not differ from the nontreated in seeds per boll.
Reproductive abnormalities in glyphosate-resistant cotton caused by lower CP4-EPSPS levels in the male reproductive tissue
- Wendy A. Pline, Ryan Viator, John W. Wilcut, Keith L. Edmisten, Judith Thomas, Randy Wells
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- Journal:
- Weed Science / Volume 50 / Issue 4 / August 2002
- Published online by Cambridge University Press:
- 20 January 2017, pp. 438-447
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Glyphosate treatments to glyphosate-resistant (GR) cotton have been associated with poor pollination and increased boll abortion. Anatomical studies were conducted to characterize the effect of glyphosate treatments on the development of male and female reproductive organs of cotton flowers at anthesis. In comparison with nontreated plants, glyphosate applied at both the four-leaf stage postemergence (POST) and at the eight-leaf stage POST directed inhibited the elongation of the staminal column and filament, which increased the distance from the anthers to the receptive stigma tip by 4.9 to 5.7 mm during the first week of flowering. The increased distance from the anthers to the stigma resulted in 42% less pollen deposited on stigmas of glyphosate-treated plants than in nontreated plants. Moreover, pollen from glyphosate-treated plants showed numerous morphological abnormalities. Transmission electron microscopy showed the presence of large vacuoles, numerous starch grains, and less organized pockets of the endoplasmic reticulum containing fewer ribosomes in pollen from glyphosate-treated plants than from nontreated plants. Pollen development in glyphosate-treated plants is likely inhibited or aborted at the vacuolate microspore and vacuolate microgamete stages of microgametogenesis, resulting in immature pollen at anthesis. Although stigmas from glyphosate-treated plants were 1.2 to 1.4 mm longer than those from nontreated plants, no other anatomical differences in stigmas were visibly evident. The presence of the GR 5-enolpyruvylshikimate-3-phosphate synthase (CP4-EPSPS) enzyme from Agrobacterium sp. strain CP4 was quantified in reproductive and vegetative tissues using enzyme-linked immunosorbent assay. The content of CP4-EPSPS in the stigma, anther, preanthesis floral bud (square), and flower petals was significantly less than that in the vegetative leaf tissue. Glyphosate effects on the male reproductive development resulting in poor pollen deposition on the stigma, as well as production of aborted pollen with reduced viability, provide a likely explanation for reports of increased boll abortion and pollination problems in glyphosate-treated GR cotton.
Glyphosate-induced reductions in pollen viability and seed set in glyphosate-resistant cotton and attempted remediation by gibberellic acid (GA3)
- Wendy A. Pline, Keith L. Edmisten, John W. Wilcut, Randy Wells, Judith Thomas
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- Journal:
- Weed Science / Volume 51 / Issue 1 / February 2003
- Published online by Cambridge University Press:
- 20 January 2017, pp. 19-27
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Glyphosate treatments to glyphosate-resistant (GR) cotton can cause increased fruit loss compared with untreated plants, likely due to reductions in pollen viability and alterations in floral morphology that may reduce pollination efficiency. This study was conducted to determine whether both stamen and pistil are affected by glyphosate treatments by measuring seed set from reciprocal reproductive crosses made between glyphosate-treated GR, untreated GR, and conventional nontransgenic cotton. Pollen viability was 51 and 38% lower for the first and second week of flowering, respectively, in GR plants treated with a four-leaf postemergence (POST) treatment and an eight-leaf POST-directed treatment of glyphosate than in GR plants that were not treated. Seed set per boll was significantly reduced when the pollen donor parent was glyphosate treated vs. untreated for the first 2 wk of flowering. There were no significant differences between treatments applied to male parents as measured by seed set at Weeks 3 and 4 of flowering. Seed set was not influenced by glyphosate treatments applied to female parents at any time. Retention of bolls resulting from crosses was reduced by glyphosate treatment of male parents during the first and third week of flowering but was not affected by glyphosate treatment of female parents. The application of gibberellic acid (GA), which has been shown to reverse male sterility in tomato (Lycopersicon esculentum L.) and to enhance boll retention in cotton, was investigated for similar effects in glyphosate-treated GR cotton. The GA treatments to glyphosate-treated plants increased the anther–stigma distance 12-fold, stigma height, and pollen viability in the second week of flowering but decreased the number of seeds in second-position bolls on Fruiting branches 1 through 3, decreased the number of first-position bolls per plant, and increased the number of squares in comparison with glyphosate-treated GR plants not receiving GA. Although GA applications to glyphosate-treated GR cotton have some remedial effect on pollen viability, the GA-induced increase in the anther–stigma difference exacerbates the increase in anther–stigma distance caused by glyphosate, resulting in low pollination.
Glyphosate negatively affects pollen viability but not pollination and seed set in glyphosate-resistant corn
- Walter E. Thomas, Wendy A. Pline-Srnić, Judith F. Thomas, Keith L. Edmisten, Randy Wells, John W. Wilcut
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- Journal:
- Weed Science / Volume 52 / Issue 5 / October 2004
- Published online by Cambridge University Press:
- 20 January 2017, pp. 725-734
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Experiments were conducted in the North Carolina State University Phytotron greenhouse and field locations in Clayton, Rocky Mount, and Lewiston-Woodville, NC, in 2002 to determine the effect of glyphosate on pollen viability and seed set in glyphosate-resistant (GR) corn. Varieties representing both currently commercial GR corn events, GA21 and NK603, were used in phytotron and field studies. All glyphosate treatments were applied at 1.12 kg ai ha−1 at various growth stages. Regardless of hybrid, pollen viability was reduced in phytotron and field studies with glyphosate treatments applied at the V6 stage or later. Scanning electron microscopy of pollen from affected treatments showed distinct morphological alterations correlating with reduced pollen viability as determined by Alexander stain. Transmission electron microscopy showed pollen anatomy alterations including large vacuoles and lower starch accumulation with these same glyphosate treatments. Although pollen viability and pollen production were reduced in glyphosate treatments after V6, no effect on kernel set or yield was found among any of the reciprocal crosses in the phytotron or field studies. There were also no yield differences among any of the hand self-pollinated (nontreated male × nontreated female, etc.) crosses. Using enzyme-linked immunosorbent assay to examine CP4-5-enolpyruvlshikimate-3-phosphate synthase expression in DKC 64-10RR (NK603) at anthesis, we found the highest expression in pollen with progressively less in brace roots, ear leaf, anthers, roots, ovaries, silks, stem, flag leaf, and husk.
Weed-Free Yield Response of Seven Cotton (Gossypium hirsutum) Cultivars to CGA-362622 Postemergence
- Dunk Porterfield, John W. Wilcut, Scott B. Clewis, Keith L. Edmisten
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- Journal:
- Weed Technology / Volume 16 / Issue 1 / March 2002
- Published online by Cambridge University Press:
- 20 January 2017, pp. 180-183
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Field studies were conducted in 1998 and 1999 to evaluate the response of seven cotton cultivars to CGA-362622 applied postemergence at 7.5 and 15 g ai/ha to three- to five-leaf cotton. The cultivars evaluated included Deltapine 51, Deltapine NuCotn 33B, Paymaster 1220 RR, Paymaster 1220 BG/RR, Stoneville bromoxynil-resistant 47, Stoneville 474, and Sure-Grow 125. At 1 to 2 wk after treatment (WAT), CGA-362622 at 7.5 and 15 g/ha injured all cotton cultivars 7 to 9% and 13 to 15%, respectively. Cotton injury symptoms included chlorosis and minor stunting. At 3 to 4 WAT, injury from CGA-362622 at 7.5 and 15 g/ha was 2 to 6% and 7 to 9%, respectively. Except for Paymaster 1220 RR, Deltapine NuCotn 33B, and Stoneville 474, all cotton cultivars were injured more by the higher rate than by the lower rate of CGA-362622. Injury was not visibly apparent 6 to 8 WAT. CGA-362622 at either rate had no effect on cotton lint yield.
Postemergence Weed Control in Soybean (Glycine max) with Cloransulam-Methyl and Diphenyl Ether Tank-Mixtures
- Wendy A. Pline, John W. Wilcut, Keith L. Edmisten
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- Journal:
- Weed Technology / Volume 16 / Issue 4 / December 2002
- Published online by Cambridge University Press:
- 20 January 2017, pp. 737-742
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Field studies were conducted in 1995 and 1996 at three locations in North Carolina to evaluate weed control and soybean injury with postemergence (POST) treatments of cloransulam-methyl alone or in tank-mixture with acifluorfen, fomesafen, or lactofen compared with a commercial standard of acifluorfen plus bentazon. Soybean injury was 2 to 3% 7 d after treatment with cloransulam-methyl applied alone and 11 to 46% when applied with fomesafen, lactofen, acifluorfen, or acifluorfen plus bentazon. Cloransulam-methyl applied alone controlled 95% of entireleaf morningglory and ivyleaf morningglory. Control was not increased by the addition of acifluorfen, fomesafen, or lactofen. Cloransulam-methyl improved the control of common lambsquarters to at least 81% compared with dimethenamid applied preemergence alone (69% control). All diphenyl ether herbicide treatments controlled common lambsquarters at least 91%. Prickly sida control by cloransulam-methyl ranged from 14 to 73% 8 wk after treatment. Control of prickly sida was varied by diphenyl ether herbicides (73 to 100% control). Tank-mixtures of cloransulam-methyl + fomesafen and cloransulam-methyl + acifluorfen increased the control of prickly sida over either herbicide applied alone. Soybean yield was greater for all tank-mixtures than for any diphenyl ether herbicide or for cloransulam-methyl treatment applied alone. But only the acifluorfen + cloransulam-methyl treatment had higher economic returns than the cloransulam-methyl treatment alone. All other POST systems, with the exception of lactofen applied alone, had similar economic returns. Tank-mixtures of cloransulam-methyl and diphenyl ether herbicides increased the spectrum of control and soybean yield compared with these herbicides applied alone.
Cotton, Peanut, and Soybean Response to Sublethal Rates of Dicamba, Glufosinate, and 2,4-D
- Virginia A. Johnson, Loren R. Fisher, David L. Jordan, Keith E. Edmisten, Alexander M. Stewart, Alan C. York
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- Journal:
- Weed Technology / Volume 26 / Issue 2 / June 2012
- Published online by Cambridge University Press:
- 20 January 2017, pp. 195-206
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Development and utilization of dicamba-, glufosinate-, and 2,4-D-resistant crop cultivars will potentially have a significant influence on weed management in the southern United States. However, off-site movement to adjacent nontolerant crops and other plants is a concern in many areas of eastern North Carolina and other portions of the southeastern United States, especially where sensitive crops are grown. Cotton, peanut, and soybean are not resistant to these herbicides, will most likely be grown in proximity, and applicators will need to consider potential adverse effects on nonresistant crops when these herbicides are used. Research was conducted with rates of glufosinate, dicamba, and 2,4-D designed to simulate drift on cotton, peanut, and soybean to determine effects on yield and quality and to test correlations of visual estimates of percent injury with crop yield and a range of growth and quality parameters. Experiments were conducted in North Carolina near Lewiston-Woodville and Rocky Mount during 2009 and 2010. Cotton and peanut (Lewiston-Woodville and Rocky Mount) and soybean (two separate fields [Rocky Mount] during each year were treated with dicamba and the amine formulation of 2,4-D at 1/2, 1/8, 1/32, 1/128, and 1/512 the manufacturer's suggested use rate of 280 g ai ha−1 and 540 g ai ha−1, respectively. Glufosinate was applied at rates equivalent to 1/2, 1/4, 1/8, 1/16, and 1/32 the manufacturer's suggested use rate of 604 g ai ha−1. A wide range of visible injury was noted at both 1 and 2 wk after treatment (WAT) for all crops. Crop yield was reduced for most crops when herbicides were applied at the highest rate. Although correlations of injury 1 and 2 WAT with yield were significant (P ≤ 0.05), coefficients ranged from −0.25 to −0.50, −0.36 to −0.62, and −0.40 to −0.67 for injury 1 WAT vs. yield for cotton, peanut, and soybean, respectively. These respective crops had ranges of correlations of −0.17 to −0.43, −0.34 to −0.64, and −0.41 to −0.60 for injury 2 WAT. Results from these experiments will be used to emphasize the need for diligence in application of these herbicides in proximity to crops that are susceptible as well as the need to clean sprayers completely before spraying sensitive crops.
Absorption and translocation of glyphosate in glyphosate-resistant cotton as influenced by application method and growth stage
- Wendy A. Pline, Andrew J. Price, John W. Wilcut, Keith L. Edmisten, Randy Wells
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- Journal:
- Weed Science / Volume 49 / Issue 4 / August 2001
- Published online by Cambridge University Press:
- 20 January 2017, pp. 460-467
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The influence of herbicide placement and plant growth stage on the absorption and translocation patterns of 14C-glyphosate in glyphosate-resistant cotton was investigated. Plants at four growth stages were treated with 14C-glyphosate on a 5-cm2 section of the stem, which simulated a postemergence-directed spray (PDS) application, or on the newest mature leaf, which simulated a postemergence (POST) application. Plants were harvested 3 and 7 d after treatment and divided into the treated leaf or treated stem, mature leaves, immature leaves and buds, stems, roots, fruiting branches (including the foliage on the fruiting branch), squares, and bolls. The PDS versus POST application main effect on absorption was significant. Absorption of 14C-glyphosate applied to stem tissue was higher in PDS applications than in POST applications. Plants receiving PDS applications absorbed 35% of applied 14C-glyphosate, whereas those receiving POST applications absorbed 26%, averaged over growth stages at application. Absorption increased from the four-leaf growth stage to the eight-leaf stage in POST applications but reached a plateau at the eight-leaf stage. Plants with PDS applications showed an increase in absorption from the four- to eight- to twelve-leaf stages and reached a plateau at the 12-leaf stage. Translocation of 14C-glyphosate to roots was greater at all growth stages with PDS treatments than with POST treatments. Herbicide placement did not affect translocation of 14C-glyphosate to squares and bolls. Squares and bolls retained 0.2 to 3.7% of applied 14C-glyphosate, depending on growth stage. Separate studies were conducted to investigate the fate of foliar-applied 14C-glyphosate at the four- or eight-leaf growth stages when harvested at 8- or 10-leaf, 12-leaf, midbloom (8 to 10 nodes above white bloom), and cutout (five nodes above white bloom, physiological maturity) stages. Thirty to 37% of applied 14C-glyphosate remained in the plant at cutout in four- and eight-leaf treatment stages, respectively. The concentration of 14C-glyphosate in tissue (Bq g−1 dry weight basis) was greatest in mature leaves and immature leaves and buds in plants treated at the four-leaf stage. Plants treated at the eight-leaf stage and harvested at all growth stages except cutout showed a higher concentration of 14C-glyphosate in squares than in other plant tissue. Accumulation of 14C-glyphosate in squares reached a maximum of 43 Bq g−1 dry weight at harvest at the 12-leaf stage. This concentration corresponds to 5.7 times greater accumulation of 14C-glyphosate in squares than in roots, which may also be metabolic sinks. These data suggest that reproductive tissues such as bolls and squares can accumulate 14C-glyphosate at higher concentrations than other tissues, especially when the herbicide treatment is applied either POST or PDS during reproductive stages (eight-leaf stage and beyond).
Yield and Physiological Response of Nontransgenic Cotton to Simulated Glyphosate Drift
- Walter E. Thomas, Ian C. Burke, Bridget L. Robinson, Wendy A. Pline-Srnić, Keith L. Edmisten, Randy Wells, John W. Wilcut
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- Journal:
- Weed Technology / Volume 19 / Issue 1 / March 2005
- Published online by Cambridge University Press:
- 20 January 2017, pp. 35-42
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Field studies were conducted in 2001 in Lewiston, NC, and in 2002 at Clayton and Lewiston, NC, to investigate the response of nontransgenic cotton to simulated glyphosate drift in a weed-free environment. Nontransgenic cotton variety ‘Fibermax 989’ was planted in a conventional seedbed at all locations. Glyphosate treatments were applied early postemergence (EPOST) at the four-leaf growth stage of cotton at 0, 8.7, 17.5, 35, 70, 140, 280, 560, and 1,120 g ai/ha and represent 0, 0.78, 1.55, 3.13, 6.25, 12.5, 25, 50, and 100% of the commercial use rate, respectively. Rates as low as 140 g/ha caused lint yield reductions depending on year and location. When averaged over all locations, lint yield reductions of 4, 49, 72, and 87% compared with nontreated cotton were observed with glyphosate rates of 140, 280, 560, and 1,120 g/ha, respectively. Visual injury and shikimic acid accumulation were evident at glyphosate rates greater or equal to 70 g/ha. Collectively, visual injury and shikimic acid accumulation at 7 d after EPOST treatment might be used as a diagnostic indicator to predict potential yield reductions from simulated glyphosate drift.
Cotton Stage of Growth Determines Sensitivity to 2,4-D
- Seth A. Byrd, Guy D. Collins, A. Stanley Culpepper, Darrin M. Dodds, Keith L. Edmisten, David L. Wright, Gaylon D. Morgan, Paul A. Baumann, Peter A. Dotray, Misha R. Manuchehri, Andrea Jones, Timothy L. Grey, Theodore M. Webster, Jerry W. Davis, Jared R. Whitaker, Phillip M. Roberts, John L. Snider, Wesley M. Porter
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- Journal:
- Weed Technology / Volume 30 / Issue 3 / September 2016
- Published online by Cambridge University Press:
- 20 January 2017, pp. 601-610
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The anticipated release of EnlistTM cotton, corn, and soybean cultivars likely will increase the use of 2,4-D, raising concerns over potential injury to susceptible cotton. An experiment was conducted at 12 locations over 2013 and 2014 to determine the impact of 2,4-D at rates simulating drift (2 g ae ha−1) and tank contamination (40 g ae ha−1) on cotton during six different growth stages. Growth stages at application included four leaf (4-lf), nine leaf (9-lf), first bloom (FB), FB + 2 wk, FB + 4 wk, and FB + 6 wk. Locations were grouped according to percent yield loss compared to the nontreated check (NTC), with group I having the least yield loss and group III having the most. Epinasty from 2,4-D was more pronounced with applications during vegetative growth stages. Importantly, yield loss did not correlate with visual symptomology, but more closely followed effects on boll number. The contamination rate at 9-lf, FB, or FB + 2 wk had the greatest effect across locations, reducing the number of bolls per plant when compared to the NTC, with no effect when applied at FB + 4 wk or later. A reduction of boll number was not detectable with the drift rate except in group III when applied at the FB stage. Yield was influenced by 2,4-D rate and stage of cotton growth. Over all locations, loss in yield of greater than 20% occurred at 5 of 12 locations when the drift rate was applied between 4-lf and FB + 2 wk (highest impact at FB). For the contamination rate, yield loss was observed at all 12 locations; averaged over these locations yield loss ranged from 7 to 66% across all growth stages. Results suggest the greatest yield impact from 2,4-D occurs between 9-lf and FB + 2 wk, and the level of impact is influenced by 2,4-D rate, crop growth stage, and environmental conditions.
Response of LibertyLink and WideStrike Cotton to Varying Rates of Glufosinate
- Darrin M. Dodds, Christopher L. Main, L. Thomas Barber, Charles Burmester, Guy D. Collins, Keith Edmisten, Daniel O. Stephenson IV, Jared R. Whitaker, Deborah L. Boykin
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- Journal:
- Weed Technology / Volume 29 / Issue 4 / December 2015
- Published online by Cambridge University Press:
- 20 January 2017, pp. 665-674
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Field studies were conducted in Alabama, Arkansas, Georgia, Louisiana, Mississippi, North Carolina, and Tennessee during 2010 and 2011 to determine the effect of glufosinate application rate on LibertyLink and WideStrike cotton. Glufosinate was applied in a single application (three-leaf cotton) or sequential application (three-leaf followed by eight-leaf cotton) at 0.6, 1.2, 1.8, and 2.4 kg ai ha−1. Glufosinate application rate did not affect visual injury or growth parameters measured in LibertyLink cotton. No differences in LibertyLink cotton yield were observed because of glufosinate application rate; however, LibertyLink cotton treated with glufosinate yielded slightly more cotton than the nontreated check. Visual estimates of injury to WideStrike cotton increased with each increase in glufosinate application rate. However, the injury was transient, and by 28 d after the eight-leaf application, no differences in injury were observed. WideStrike cotton growth was adversely affected during the growing season following glufosinate application at rates of 1.2 kg ha−1 and greater; however, cotton height and total nodes were unaffected by glufosinate application rate at the end of the season. WideStrike cotton maturity was delayed, and yields were reduced following glufosinate application at rates of 1.2 kg ha−1 and above. Fiber quality of LibertyLink and WideStrike cotton was unaffected by glufosinate application rate. These data indicate that glufosinate may be applied to WideStrike cotton at rates of 0.6 kg ha−1 without inhibiting cotton growth, development, or yield. Given the lack of injury or yield reduction following glufosinate application to LibertyLink cotton, these cultivars possess robust resistance to glufosinate. Growers are urged to be cautious when increasing glufosinate application rates to increase control of glyphosate-resistant Palmer amaranth in WideStrike cotton. However, glufosinate application rates may be increased to maximum labeled rates when making applications to LibertyLink cotton without fear of reducing cotton growth, development, or yield.