Westward expansion of the Patagonian steppe and retrocession of Andean forests due to increasing aridity over the past one or two millennia has been a persistent theme in the ecological and paleoecological literature for at least half a century. New evidence from pollen profiles, tree-ring analysis, vegetation structure, and photographic and documentary historical sources does not show the expansion of the steppe. Instead, over the past century trees have invaded the steppe as a consequence mainly of human-induced changes in the fire regime, and trees have regenerated in forest areas that were heavily burnt at the onset of European colonization.

Advancements in ancient DNA analyses now permit comparative molecular and morphological studies of extinct animal dung commonly preserved in caves of semiarid regions. These new techniques are showcased using a unique dung deposit preserved in a late glacial vizcacha (Lagidium sp.) midden from a limestone cave in southwestern Argentina (38.5° S). Phylogenetic analyses of the mitochondrial DNA show that the dung originated from a small ground sloth species not yet represented by skeletal material in the region, and not closely related to any of the four previously sequenced extinct and extant sloth species. Analyses of pollen and plant cuticles, as well as analyses of the chloroplast DNA, show that the Cuchillo Curá ground sloth browsed on many of the same herb, grass, and shrub genera common at the site today, and that its habitat was treeless Patagonian scrub-steppe. We envision a day when molecular analyses are used routinely to supplement morphological identifications and possibly to provide a time-lapse view of molecular diversification.

Paleoenvironmental changes dating back to before 10,000 yr B.P. at the northernmost occurrences of Nothofagus forests in Argentina at about latitude 37°S permit the reconstruction of past changes in the intensity of the winter rains, related to the southern westerlies that appear to determine the forest boundary. The paleoenvironmental interpretation is based on changes in the proportions of different Nothofagus species and changes in the ratio betwen forest and steppe taxa. The relatively most diverse and dense Nothofagus forest developed only during the last 4500 yr, prior to human impact during the last 300 yr. Before the middle Holocene, climatic conditions must have been different from the modern ones, with less overall precipitation judging from the overall lower amount of tree pollen and the reduction to primarily Nothfagus pumilio. An interval dated to older than 10,000 yr B.P. is characterized by co-occurrence of Prumnopitys andina, Nothofagus pumilio, and shrub-steppe taxa. Prumnopitys andina is known today only from scattered upper montane forest sites in Chile between 36° and 43°S lat and its ecological requirements are essentially unknown. The taxa combination, however, suggests that late-glacial climate must have been drier, and probably cooler than today. This implies that the winter rains and, consequently, the seasonal shift of the westerly circulation was reduced during the late Pleistocene and did not reach modern levels before 8500 yr B.P.

Pollen assemblage changes and stable hydrogen isotope analysis of mosses (Sphagnum magellanicum and Drepanocladus s.l.) from a bog in Tierra del Fuego, Argentina, provided independent proxies for reconstructing changes in effective moisture and temperature over the past 16,000 cal yr B.P. A deterministic model was used to reconstruct the stable hydrogen isotope composition of meteoric water from the D/H ratios of bog mosses over the last 16,000 years. Abrupt changes in temperature, as recorded in D/H ratios of moss cellulose, were accompanied by synchronous changes in vegetation composition during the late Pleistocene and early and middle Holocene, when moisture levels were lower than today. In contrast, temperature variability during the late Holocene was not accompanied by comparable vegetation changes. In particular, grass pollen (Poaceae) increased during periodic cold spells between 15,000 and 11,000 cal yr B.P., but a cold spell of similar magnitude ca. 2000 cal yr B.P. did not appear to affect vegetation. During the late Pleistocene, the isotopic record from the peat core shows variations similar to the D/H ratios in the Antarctic Taylor Dome ice core. However, the timing of the changes in the Harberton record is more in line with the timing of other Southern Hemisphere records.

Paleomagnetic analysis of samples from the original 600-m-long core from the San Agustin Plains, New Mexico, showed an unquestionable reversal stratigraphy dating the record back to at least 1.6 my. Analysis of pollen, ostracodes, and algae of a duplicate sample section in the vicinity of the original coring site and spanning the last 18,000 yr B.P. shows a much higher resolution than the earlier results.

The history of the low-elevation forest and forest-steppe ecotone on the east side of the Andes is revealed in pollen and charcoal records obtained from mid-latitude lakes. Prior to 15,000 cal yr BP, the vegetation was characterized by steppe vegetation with isolated stands of Nothofagus. The climate was generally dry, and the sparse vegetation apparently lacked sufficient fuels to burn extensively. After 15,000 cal yr BP, a mixture of Nothofagus forest and shrubland/steppe developed. Fire activity increased between 13,250 and 11,400 cal yr BP, contemporaneous with a regionally defined cold dry period (Huelmo/Mascardi Cold Reversal). The early-Holocene period was characterized by an open Nothofagus forest/shrubland mosaic, and fire frequency was high in dry sites and low in wet sites; the data suggest a sharp decrease in moisture eastward from the Andes. A shift to a surface-fire regime occurred at 7500 cal yr BP at the wet site and at 4400 cal yr BP at the dry site, preceding the expansion of Austrocedrus by 1000–1500 yr. The spread of Austrocedrus is explained by a shift towards a cooler and wetter climate in the middle and late Holocene. The change to a surface-fire regime is consistent with increased interannual climate variability and the onset or strengthening of ENSO. The present-day mixed forest dominated by Nothofagus and Austrocedrus was established in the last few millennia.