Book contents
- Analytical Geomicrobiology A Handbook of Instrumental Techniques
- Analytical Geomicrobiology
- Copyright page
- Contents
- Contributors
- Foreword
- Part I Standard Techniques in Geomicrobiology
- Part II Advanced Analytical Instrumentation
- Part III Imaging Techniques
- Part IV Spectroscopy
- Part V Microbiological Techniques
- 14 Lipid Biomarkers in Geomicrobiology: Analytical Techniques and Applications
- 15 Phylogenetic Techniques in Geomicrobiology
- Index
- References
15 - Phylogenetic Techniques in Geomicrobiology
from Part V - Microbiological Techniques
Published online by Cambridge University Press: 06 July 2019
- Analytical Geomicrobiology A Handbook of Instrumental Techniques
- Analytical Geomicrobiology
- Copyright page
- Contents
- Contributors
- Foreword
- Part I Standard Techniques in Geomicrobiology
- Part II Advanced Analytical Instrumentation
- Part III Imaging Techniques
- Part IV Spectroscopy
- Part V Microbiological Techniques
- 14 Lipid Biomarkers in Geomicrobiology: Analytical Techniques and Applications
- 15 Phylogenetic Techniques in Geomicrobiology
- Index
- References
Summary
Molecular biological techniques have revolutionized the field of geomicrobiology by providing researchers with robust techniques for identifying microorganisms and characterizing microbial communities in a wide variety of environments. These techniques have freed researchers from the constraints of classical culture-based microbiology and allowed the discovery of previously unknown phylogenetic diversity of microorganisms. In this chapter, we discuss the theory, methods, and workflow for applying molecular techniques to identify and characterize microbial populations. Our chapter focuses on SSU rRNA gene-based approaches, guiding the reader from sample collection and gene amplification through bioinformatics and statistical analysis. The workflow presented has been successfully used to identify microbial populations and community dynamics in a wide variety of habitats to understand the interactions between microbes and their environment.
- Type
- Chapter
- Information
- Analytical GeomicrobiologyA Handbook of Instrumental Techniques, pp. 360 - 404Publisher: Cambridge University PressPrint publication year: 2019