The World’s Poultry Science Journal is indebted to Prof J.A. Castello, Prof D.K. Flock, Dr M. Tixier-Boichard, Dr S. Cherepanov and Dr Jiangxia Zheng for the translations of these summaries.

Traditional diagnostic capabilities (serology and culture) are not enough to monitor the poultry infections efficiently. For effective control of poultry infections, a regular program incorporating simple and cost-effective molecular diagnostics is necessary. On this rationale, it is possible to present working molecular diagnostic technology that would work equally well in field as well as in the laboratory. Recently, the loop-mediated isothermal amplification (LAMP) assay has emerged as simple and inexpensive diagnostic tool for the molecular detection of various animal pathogens. To perform LAMP, no specialised instruments (e.g. thermal cycler) are required, permitting its use in developing countries. Various reliable LAMP assays have been reported for the detection of different poultry pathogens. However, still there is a need to improve the sensitivity, specificity, reproducibility, user-friendliness, delivery to end-user and affordability of LAMP assays. This article reviews current LAMP assays available for the molecular detection of important viral, bacterial and protozoan pathogens of poultry. It focuses on the various aspects of LAMP for the diagnosis of important poultry pathogens based upon pathogen type, specimen, target genes, LAMP primer types, detection limits, fluorescent detectors and LAMP chemistry used. This paper provides updates on principle, instrumentation, basic methodology, quantification capability, reagents and kits used currently in performing LAMP.

Studies have shown that dietary chromium (Cr) supplementation beneficially affects physiological functions such as cell preservation, antioxidant activity and immune response that are of utmost importance to animal homeostasis and thermoregulatory capacity under heat stress conditions. For instance, Cr is essential for the synthesis of the specific low molecular weight Cr-binding-substance (LMWCr) that upon conversion to chromodulin, activates the insulin signalling cascade. This results in greater cell permeability to insulin, with a subsequent positive effect on the metabolism of carbohydrates, lipids and proteins. Furthermore, Cr has antioxidant properties which help to attenuate the negative effects of oxidative stress. With regards to meat quality, Cr decreases lipid peroxidation. It has been suggested that Cr supplementation increases antibody responses and lymphocyte counts in broiler chickens exposed to high environmental temperatures. In addition, trivalent Cr supplementation increases growth performance and decreases the circulating levels of undesirable metabolites and hormones such as cholesterol and corticosterone in broiler chickens exposed to heat stress. The aim of this review was to report the effects of Cr supplementation as a nutritional strategy to increase growth performance, immune response, carcass characteristics and meat quality of broilers produced under hot conditions. This knowledge may contribute to improve the productivity and sustainability of broiler production in a context of global warming and development of livestock production in hot climate areas.

In the adult hen, the oviduct receives the ovum from the ovary and provides the biological environment for the formation and potential fertilisation of the egg. During egg formation, albumin, from the magnum is deposited around the yolk, followed by the eggshell membranes from the isthmus, which subsequently surround the egg. As the yolk traverses through the oviduct, calcium is deposited on to it, from the uterus, forming a hardened eggshell. Ovalbumin, avidin and ovomucin secreted from magnum provides content and antimicrobial activity in the egg-white. Collagen X and fibrilin 1 from the isthmus make up the fibrous eggshell membranes. Calbindin 1, ovocleidin-116 and secreted phosphoprotein 1 secreted from the shell gland contribute in calcium ion remodelling for eggshell mineralisation. This review summarises the expression pattern and functional role of genes having a leading role in the egg formation.

Increasing the structural components in the diet, namely through including coarse grain particles in diets and manipulating the dietary fibre composition, has been shown to improve gut health, feed utilisation and production efficiency. This is primarily because structural components physically stimulate activity in the fore gut. An example of this is dietary non-starch polysaccharides (NSP), namely insoluble NSP, which have been shown to instigate beneficial effects on gut health, litter quality and nutrient utilisation, by increasing crop and gizzard activity, stimulating digestive enzyme production and enhancing bacterial fermentation in the hind gut. However, there is a lack of consistency with regard to the direct effects of dietary fibre on chicken health and production. The aim of this review therefore is to explore the impact of feeding different sources of fibre and different size grain particles on gut health and microflora, nutrient utilisation, performance and litter quality in broilers.

Birds have evolved in direct contact with natural nanoparticles (NPs) that are identical to artificial trace-element NPs. This relationship, the high action potential and their ability to reduce environmental pollution make NPs a promising component of bird diets. However, from available published studies there is no unity in justifying the applied dosages of NPs and their calculations. NPs are used in the studies in various doses, for example: Cu 0.5-50 mg/kg, Ag 10-1000 mg/kg, Se 0.2-5 mg/kg, Cr 500-1500 ppb. Therefore, universal approaches and criteria of NP investigations are necessary for the establishment of their use in feed.

The mechanisms of action of the trace elements in artificial NPs in birds vary from the those of ionic forms of trace elements, which determine the differences in the productive effect. According to data from different authors, chickens receiving NPs in feed have higher chickens body weight by 13-24%. Such benefits have increased interest in sources of trace-element NPs significantly over the past two decades. The design of trace-element NPs has led to promising developments in the safe use of NPs for poultry nutrition, such as coating NPs with inert substances and adjusting their size. However, constraining circumstances determined by the difficulty of predicting the toxic properties of nanostructures exist, even though artificial trace-element NPs are a relatively safe class of nanostructures due to their production requirements, and metal NPs are already used in human food and medicine. The following review discusses the benefits and potential hazardous effects of NPs and the possibility of using them as feed supplements for poultry.

Heat stress is one of the main challenges in poultry production as it reduces performance in broilers and layers. This review focuses on the impact of heat stress in poultry production with emphasis on broilers and layers, methodologies to measure the severity of stress and dealing the preventive measures to alleviate stress due to heat. Potential use of naked neck and frizzle genes is highlighted. Nutritional interventions including offering a balanced diet, increasing energy, required amino acids, vitamins like vitamins A, E, Se and minerals like Ca, Na, Cl and K additional supplementation of vitamin C, provision of cool water at levels of up to five times of feed intake to satisfy the special needs during heat stress all have proven advantages. Designing housing with gable type roofing and open sided, wet curtains/exhaust fans, tunnel ventilation in environment control houses, provision of more floor space and free movement, to exhibit natural movement and decreasing flock density is useful to minimise stress as well as to regulate the temperature in micro environment. Thermos tolerance in birds can be enhanced by early feed restriction, good management with better ventilation and developing of heat tolerant breeds by selecting for less feathers. Selection of breeds suitable to climate, nutritional manipulation and small management changes can minimise heat stress in birds.

Pigeon pea (PP), Cajanus cajan, is a plant that is cultivated for human food and animal feed. It exists as a wide range of cultivars, and their flexibility for use in animal rearing systems have made PP popular, especially for small-scale farmers. PP is grown widely in India and in parts of Africa and Central America. The main producers of PP in the world are India, Uganda, Tanzania, Kenya, Malawi, Ethiopia, Mozambique, the Dominican Republic, Puerto Rico, the West Indies in the Caribbean and Latin America region, Indonesia and the Philippines and Australia. Analysis has shown that PP contains 17.9-24.3% crude protein (CP) in whole grain, and 21.1-28.1% in split seeds, and high protein genotypes contain 32.5%. Optimal levels of utilisation have been shown to improve broiler performance and may reduce daily feed cost. However, PP contains anti-nutritional factors that negatively affect feed efficiency. The use of processing methods such as fermentation, boiling, milling, soaking, and roasting can minimise any harmful effects and improve its nutritive quality, positively enhancing performance parameters. Studies on the use of PP suggested that it can be included at 7.5% of the diet or as 50% substitution for soybean meal in broiler diets.

Paul Hocking

1948 - 2018

Paul Hocking was born in 1948 and grew up on a mixed farm near Exeter in Devon. He read agriculture at Reading University and obtained a postgraduate Diploma in Genetics at Edinburgh University in 1970. From 1970 to 1977 he worked for a secretariat providing services to cattle breeding societies. His work on a selection programme for dairy shorthorn cattle formed the basis for his PhD awarded in 1978 by Reading University. After 3 years lecturing at Reading he spent the next two years as a research fellow at the Animal Research Centre in Ottawa. It was there that he started to transfer his genetic interests from cattle to poultry. In 1983 he joined the Nutrition Department at the Poultry Research Centre in Edinburgh with the remit to study the topic of feed restriction in breeding birds. He remained there for the rest of his career seeing many changes, with the centre by the time of his retirement having been absorbed into the Roslin Institute and subsequently the Royal (Dick) School of Veterinary Science in the University of Edinburgh.

Paul quickly made a name in what became known as the ‘broiler breeder paradox’. The large body of work that defined the reproductive biology of broiler breeders and its control by feed restriction made him the go-to person for broiler and turkey breeder reproductive and welfare research. All Paul’s work was characterised by well-designed experiments and careful conclusions that led to sound understanding. This standing was recognised by the European Food Standard agency, with him serving on their Panels on Animal Health and Welfare of broilers and broiler breeders and in judicial reviews in the UK on breeder welfare. Paul embraced the genomic revolution and was in the forefront of setting up the populations needed to identify genes for Mendelian and quantitative traits in poultry. He found new applications for his talents in understanding eye defects and disease susceptibility. His review, published in the WPSJ in 2008, on foot pad lesion scoring remains high in the cited papers list. Paul was diligent in carrying a piece of work through to its completion and was author or co-author of over 200 papers. He was a sought-after speaker and had travelled around the world on his reputation - travelling was something he much enjoyed. His work was recognised by the award of the Gordon Memorial Medal in 2013 giving his widely acclaimed lecture on the subject of ‘The unexpected consequences of genetic selection in broilers and turkeys: problems and solutions’

Paul made a huge contribution to the committees and societies in our science community. He was a prominent figure in the UK branch of the World Poultry Science Association (WPSA). He served as its President and played an important role in several of the Poultry Science Symposia organised by the Branch. Paul also made a major contribution to the European Federation of WPSA. He was Vice President from 2006 to 2010 and the UK representative on Working Group 3 (Genetics). He organised the 7th Symposium of the Group in Scotland. He was a Council Member of British Poultry Science and in 2010 became its Joint Editor.

Paul was popular with his colleagues and with his thoughtful, friendly demeanour was a welcome collaborator on many projects. His unique style of after-dinner jokes has been imitated but not matched. His service to the science and community that underpins such a major industry has left a lasting legacy. All these things, except the jokes, were recognised when Paul was elected to the International Poultry Hall of Fame at the World Poultry Congress in Beijing in 2016. He was a great scientist, contributing hugely to poultry research, as well as a friend and mentor to many.

Paul had latterly decreased his work load to part time, preparatory to moving back to his roots in Devon. He had started his new life there, much preferring the milder climate to that of Edinburgh. It is a great pity that the rapid onset of a cancer deprived him of more years of retirement. He leaves a wife, Denise, son Chris and daughters Michelle and Jenny. He will be much missed by them and his many friends and colleagues around the world.

Dr Ian Dunn and Professor Colin Whitehead

Donald McQueen Shaver

12 August 1920 – 28 July 2018

One of the first Canadians inducted into the International Poultry Hall of Fame, Donald Shaver, founder of Shaver Poultry Breeding Farms Ltd., has died, a few days short of his 98th birthday. Donald Shaver was born and grew up in Galt, now part of Cambridge, Ontario. As a teenager he kept chickens in the backyard of his urban home, and in a vacant lot next door.

He joined the Canadian army in the second World War, achieved the rank of Lieutenant Colonel, and was part of the force that liberated The Netherlands in 1945.

After the war, he extended his interest in poultry breeding and established a hatchery and feed mill in Galt. He assembled a large collection of White Leghorn lines purchased from other breeders and began crossbreeding experiments that led to the development of the Shaver Starcross 288. The outstanding performance of this hybrid encouraged Shaver to expand his operations and begin selling parent stock to franchise hatcheries in Canada and the United States. He built a larger hatchery and established a breeding farm adjacent to his home on the outskirts of Galt. By the mid 1960’s new farms were added, and a much larger hatchery, as the business expanded around the world.

At its height, Shaver Poultry Breeding Farms Ltd. was selling breeding stock in more than 90 countries. Subsidiary companies were established in the US, Great Britain, France, and Germany. There were joint ventures in Pakistan, New Zealand, India and Barbados. The company expanded into brown egg layers and meat chickens, which were sold alongside the highly successful white egg Starcross 288. By the mid 1970’s there were four breeding farms in Cambridge, and two hatcheries. Breeding development work took place in France and Great Britain as well as in Canada.

Donald Shaver was himself responsible for a large part of the Company’s success and expansion. He travelled extensively, probably spending between one third and one half of his time overseas. While at home he worked 16 hours, seven days a week, and expected similar commitment from his staff.

Initially, most of the genetics input came from consultants, of which Dr R.K. Cole of Cornell University was the most active. In-house geneticists were hired beginning in the 1960’s and two were employed at the time of Donald Shaver’s retirement in 1985.

Early on, during a period of rapid expansion, Cargill Inc. of Minneapolis became part owners of Shaver Poultry Breeding Farms. When Donald Shaver retired in 1985, they became sole owners and soon sold the Company to ISA in France, and they in turn were taken over by what has now become Hendrix Genetics. This Company still maintains two of the breeding farms and a busy hatchery in Cambridge.

Shaver also established a beef breeding business and it became quite successful, selling frozen semen and embryos internationally. However, when one case of mad-cow disease occurred in Alberta in 1995, the international market shut down and the beef business closed.

After retirement, Donald Shaver maintained his lifelong advocacy for sustainable agriculture. He made his final presentation on this topic in 2016. He was involved as a Director in energy, insurance, communication and manufacturing. He was Chairman of Canada Development Investment Corporation until 2008.

Among many awards, Donald Shaver received honorary doctorates from the Universities of McGill, Guelph and Alberta, and was an Officer of the Order of Canada. He is survived by two sons and two daughters, grandchildren and great-grandchildren.

John Brake

1952 – 2018

NC State University’s College of Agriculture and Life Sciences lost a world-renowned poultry science expert and award-winning teacher, mentor and leader on July 31, when the William Neal Reynolds Distinguished Professor, John Brake passed away.

Brake, aged 66, had a long history with NC State and its Prestage Department of Poultry Science – first as an undergraduate studying poultry and animal science and then as a Ph.D. student in physiology in the 1970s. He joined the faculty at Auburn University in 1978, then returned to NC State as an assistant poultry science professor in 1981.

Brake’s research assistant Rasha Qudsieh noted that he was best known for his expertise in feed milling, enzymes, induced moulting of commercial layers, management and nutrition of broiler breeders and their progeny, processing and hatchery management. “He also developed and managed a truly singular vertically integrated broiler breeder-broiler research programme based at NC State for over 30 years,” she said.

Brake wrote hundreds of scientific and popular articles that have been translated into over 10 languages, and he has consulted and presented in more than 40 countries. He held several leadership roles in his department and for professional societies, and served as poultry science’s research coordinator for eight years and director of graduate programmes for 15 years.

He won many other research, teaching and international service awards, including the university’s Global Engagement Award in 2016, its Outstanding Young Alumnus Award in 1986 and CALS’ graduate instructor award in 2003. He has received two of the highest honours bestowed by the Poultry Science Association: He won the Merck Award for Achievement in 1995 and was named a fellow in 2006.

Pat Curtis, head of the Prestage Department of Poultry Science, said that Brake was "a friend, colleague, mentor and scholar (who) will be greatly missed by the department and the poultry industry."

John Brake was a long-time and very active WPSA member. He attended and actively participated in WPSA meetings/seminars around the globe and provided consulting service to many in the poultry industry in all parts of the world. He was a regular fixture at poultry meetings, and well known and respected by his fellow poultry scientists.

Dr Peter E. Lake OBE, FRSE

23 September 1928 – 14 June 2018

Dr Peter Edmund Lake passed away suddenly but peacefully on 13th June 2018 aged 89 years at a care home in Annan, Dumfriesshire, Scotland. His home of 66 years had been Edinburgh where he devoted his entire working life (1951-1988) to avian research, specifically but not exclusively relating to the domestic fowl. He established a world-wide reputation in the field of artificial insemination that had wide ranging benefits for the poultry industry in Britain and around the world.

Dr Lake had a degree in Zoology from Birmingham University (1949), a diploma in Agriculture from Christ's College, Cambridge (1950) and a PhD in avian reproductive physiology from Edinburgh University (1955). In 1951 he took a post as a Scientific Officer in the Reproductive Physiology Department at the Poultry Research Centre in Edinburgh, a body of the UK Agricultural and Food Research Council. He was to be based there all his working life ultimately being appointed Acting Head of Station.

In the post-War period there was a rapid expansion in the poultry industry as a source of cheaper food and this could not be sustained through traditional farm breeding methods. Peter Lake and colleagues pioneered work on the biochemistry and physiology of the production and function of the spermatozoa of domestic birds (especially chickens and turkeys). He recognised the importance of artificial insemination not only for the exponential growth of the poultry industry but also to enhance the quality and size of the product through selective breeding. This required much experimentation in the collection, storage and transportation of the spermatozoa. In 1952 Dr Lake spent a study year at the National Institute for Medical Research, Mill Hill, London with Professor A.S. Parkes and Dr C. Polge who had discovered the use of glycerol for freezing spermatozoa. In 1960 Dr Lake spent a sabbatical year on a Lalor Foundation Fellowship at the University of California, Davis with Prof. F.W. Lorenz and Dr F.X. Ogasawara. This was a life shaping experience from which many friendships and partnerships developed, underlined in 1989 with his election as a Fellow of the Poultry Science Association of America.

Throughout his career Dr Lake wrote or contributed to scores of scientific articles, books and reviews including in1978 (with J.M. Stewart) a key work for the Ministry of Agriculture, Fisheries and Food entitled 'Artificial Insemination in Poultry'. He travelled widely in support of colleagues around the world and to attend numerous poultry congresses usually as a speaker or lecturer. In 1980 he spent three months in Japan as Visiting Professor in Animal Science at Kyushu University, advising many poultry breeding centres.

Upon his retirement as Head of the Reproductive Physiology Department in 1988 Dr Lake was proud to receive the Order of the British Empire from the Queen in recognition of his lifetimes work. Previously he was also honoured with the Fellowship of the Royal Society of Edinburgh in 1982 and the Fellowship of the Institute of Biology in 1984.

Post-retirement in 1989 he took up a Nicholas Memorial Fellowship at Cuddy Farms in Ontario, Canada advising this multi-national turkey breeder and in December 1989 he concluded his career by accepting a United Nations FAO commission to assist the poultry breeding industry in Albania.

Dr Lake married Mary Bennett in 1954, who, over 56 years, was an indispensable part of his success and happiness. They had 4 sons, Michael, Martin, Christopher and Kenneth. Peter missed Mary badly after she passed away in 2010 and Michael also pre-deceased him. Peter is survived by three sons and nine grandchildren. Apart from work and family his great passion was rugby which he enjoyed as a player and referee.

Péter Földi

Péter Földi, 75, has passed away Monday, August 27th, 2018, following a long-term serious illness. He was Consultant and before the General Secretary of the Hungarian Poultry Product Board.

After his graduation as an agricultural engineer on the Agricultural University of Gödöllő he was working in various positions on the experimental farm of the University for 10 years. From 1980 to 1995 he worked at the Hungarian Ministry of Agriculture, between 1991-1995 as Head of Department. From 1995 he was the General Secretary of the Hungarian Poultry Product Board and the secretary of the Association of the Hungarian Layer Hybrid Breeders and Egg producers. From 2010 he was consultant for the Hungarian Poultry Product Board.

He was member in the Poultry Department of the Hungarian Veterinary Association. He was one of the editors of the Hungarian Poultry magazine as well. He represented Hungary at the International Egg Commission (IEC) for years. This organisation awarded him with a special prize in 2007.

He was the Secretary of the Hungarian Branch of the World’s Poultry Science Association (WPSA), and he was Treasurer of the EUWEP and EEPTA as well for years.

He was given several state, ministerial and sectoral awards such as ‘For Hungarian Poultry Sector’ award (2016) , ‘Újhelyi Imre’ (2011), and ‘Életfa’ award (2013), when he was 70.

The World’s Poultry Science Journal is indebted to Prof J.A. Castello, Prof D.K. Flock, Dr M. Tixier-Boichard, Dr S. Cherepanov and Prof N. Yang for the translations of these summaries.

Designer foods of animal origin are produced either by feeding specific diets, or using new techniques like genetic engineering, cross-breeding. Designer eggs are an important type of functional foods. With the advancement of poultry industry, there is a rising interest in poultry biotechnology for altering the egg composition by genetic and nutritional manipulations for human health. This can be done by modifying cholesterol concentration and its fractions, lipid profile, fatty acids, amino acids and minerals or through adding therapeutic pharmaceutical molecules. Designer eggs provide vegetarian, safe, immune powered, specialty or organic foodstuffs which can have improved vitamins, minerals, balanced ratio of omega-6 to omega-3 fatty acids, lowered total cholesterol, additional boost of antibodies and essential pigments such as carotenoids. From the relevant scientific literature, functional eggs can be considered as human designer food. This review describes the concepts of designer eggs and their health benefits and nutritional values.

Compared to 2009, total poultry population in 2016 decreased by 8.63% in Croatia. In this period, the number of hens decreased by 42.50%, turkeys by 12.31%, geese by 66.23%, ducks by 51.06%, and the number of other poultry species decreased by 90.92%. Consumption of poultry meat in all European Union countries combined is estimated at 22 kg per capita per year, which is 59.10% more than in Croatia, where poultry meat is consumed at an average level of 9 kg per capita. A similar pattern relates to the consumption of table eggs, which is 20.67% lower in Croatia than the average yearly consumption of 12.1 kg of eggs per capita in all European Union countries combined. Croatia produces 661 million eggs per year. Out of the total number of table egg producers, most of them use extensive production systems and have less than 4,999 birds at their farms, and only three producers have production capacity which exceeds 100,000 birds. The management of laying hens is mostly by using enriched caged systems. In future, the production of indigenous breeds (Zagorje turkey and Hrvatica hen) is anticipated to have an important role in Croatian poultry production, as these breeds are resistant and endurable, and have the potential to assure self-sustainability of small poultry producers.

Cinnamon is a common spice obtained from the bark of the cinnamon tree (Cinnamomum zeylanicum). It has been used for culinary, as well as medicinal, purposes since ancient times in various countries. Apart from substantial amounts of several nutrients, including carbohydrates, proteins, choline, vitamins (A, K, C, B3), and minerals, several biological active compounds are present in the extract of oil, which contribute to immunomodulatory, antioxidant, antiviral, lowering blood cholesterol, antimicrobial, lipid-lowering, antihypertension, anti-inflammatory, antitumor, gastroprotective, antidiabetic, neuroprotective and blood purifying properties. Cinnamon roots serve as a hepatic stimulant by improving bile production, eliminating toxins, restoring electrolyte balance and regulating hydration and can be used for enhancing digestion. In addition, nutritional properties of cinnamon powder include positive effects regarding growth, digestion, enhanced activity of gut microflora, improvement of immune response, as well as improved feed efficiency and health improvement of poultry birds. Recently, research focus has been directed towards supplementing broiler diets with cinnamon powder as a phytobiotic in order to replace synthetic growth promoters. After reviewing the literature, it was found that the research at the molecular level to elucidate the mechanisms behind the potential of cinnamon as a feed additive in poultry is limited, despite its promising impacts. Furthermore, supplementation doses vary significantly, i.e., from 0.02 to 7%. So, the aim of this review was to compile the published research related to cinnamon. Hence explore its beneficial properties, find out its optimal dosage for uses by veterinarians, researchers, and nutritionists, as well as its potential to use as a natural feed additive to replace the synthetic antibiotic growth promoters in poultry feed.

The World’s Poultry Science Journal is indebted to Prof J.A. Castello, Prof D.K. Flock, Dr M. Tixier-Boichard, Dr S. Cherepanov and Prof N. Yang for the translations of these summaries.

The gut has great importance for the commercial success of poultry production. Numerous ion transporters, exchangers, and channels are present on both the apical and the basolateral membrane of intestinal epithelial cells, and their differential expression along the crypt-villus axis within the various intestinal segments ensures efficient intestinal absorption and effective barrier function. Recent studies have shown that intensive production systems, microbial exposure, and nutritional management significantly affect intestinal physiology and intestinal ion transport. Dysregulation of normal intestinal ion transport is manifested as diarrhoea, malabsorption, and intestinal inflammation resulting into poor production efficiency. This review discusses the basic mechanisms involved in avian intestinal ion transport and the impact of development during growth, nutritional and environmental alterations, and intestinal microbial infections on it. The effect of intestinal microbial infections on avian intestinal ion transport depends on factors such as host immunity, pathogen virulence, and the mucosal organisation of the particular intestinal segment.