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Feed energy utilization by hair sheep: does the 0.82 conversion factor of digestible to metabolizable energy need to be revised?
- A. S. Brito Neto, C. J. L. Herbster, L. C. Geraseev, G. L. Macedo Junior, D. R. Nascimento, A. C. Rocha, M. I. B. Pereira, M. I. Marcondes, L. P. Silva, L. R. Bezerra, R. L. Oliveira, E. S. Pereira
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- Journal:
- The Journal of Agricultural Science / Volume 161 / Issue 5 / October 2023
- Published online by Cambridge University Press:
- 14 December 2023, pp. 734-742
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The objective was to evaluate energy partitioning and predict the relationship between metabolizable energy (ME) and digestible energy (DE) in hair sheep fed tropical diets at three feeding levels (maintenance, intermediate and high). To evaluate the energy partition, a database with 114 records (54 non-castrated males and 60 females) from comparative slaughter studies was used. To estimate the ratio ME:DE, 207 observations (74 non-castrated males and 133 females) were used from six studies in a multi-study approach, two indirect calorimetry studies (n = 93) and four comparative slaughter (n = 114), using a mixed model and study as random effect. A simple linear regression equation of the ME against DE was fitted to predict the efficiency of DE to ME conversion. Gas losses were greatest (P < 0.05) for animals fed at maintenance level (7.92% of gross energy intake). The variations of energy losses in the urine were 2.64, 2.06 and 2.08%; faecal losses were 34.37, 37.80 and 36.91% for maintenance, intermediary and high level of feeding, respectively. The regression analysis suggested a strong linear relationship between ME and DE, generating the model ME (MJ/day) = −0.1559 (±0.07525) + 0.8503 (±0.005864) × DE (MJ/day). This study highlights the importance of the relationship ME:DE. Equation/factor 0.85 presented herein is alternative that could be used for the calculation of ME from DE in feedlot diets tropical. In conclusion, we suggest that for hair sheep fed tropical diets the conversion factor 0.85 is more adequate to predict ME from DE.
Thinking out of the box: revisiting health surveillance based on medical records
- Vanderson S. Sampaio, Rafael Lopes, Mina Cintho Ozahata, Helder I. Nakaya, Erick Sousa, José D. Araújo, Marcelo A.S. Bragatte, Anderson F. Brito, Regina Maura Zettoni Grespan, Maria Ligia Damato Capuani, Helves Humberto Domingues, Alessandra Cristina Guedes Pellini, Sheila de Oliveira Garcia Mateos, Mônica Tilli Reis Pessoa Conde, Fabio Eudes Leal, Ester Sabino, Mariangela Simão, Jorge Kalil
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- Journal:
- Antimicrobial Stewardship & Healthcare Epidemiology / Volume 3 / Issue 1 / 2023
- Published online by Cambridge University Press:
- 24 October 2023, e185
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Despite the considerable advances in the last years, the health information systems for health surveillance still need to overcome some critical issues so that epidemic detection can be performed in real time. For instance, despite the efforts of the Brazilian Ministry of Health (MoH) to make COVID-19 data available during the pandemic, delays due to data entry and data availability posed an additional threat to disease monitoring. Here, we propose a complementary approach by using electronic medical records (EMRs) data collected in real time to generate a system to enable insights from the local health surveillance system personnel. As a proof of concept, we assessed data from São Caetano do Sul City (SCS), São Paulo, Brazil. We used the “fever” term as a sentinel event. Regular expression techniques were applied to detect febrile diseases. Other specific terms such as “malaria,” “dengue,” “Zika,” or any infectious disease were included in the dictionary and mapped to “fever.” Additionally, after “tokenizing,” we assessed the frequencies of most mentioned terms when fever was also mentioned in the patient complaint. The findings allowed us to detect the overlapping outbreaks of both COVID-19 Omicron BA.1 subvariant and Influenza A virus, which were confirmed by our team by analyzing data from private laboratories and another COVID-19 public monitoring system. Timely information generated from EMRs will be a very important tool to the decision-making process as well as research in epidemiology. Quality and security on the data produced is of paramount importance to allow the use by health surveillance systems.
The Portuguese version of the Screen for Disordered Eating: Validity and reliability in middle aged and older women
- A. T. Pereira, M. J. Brito, R. V. Duarte, C. Marques, D. Pereira, C. Cabaços, A. Macedo
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- Journal:
- European Psychiatry / Volume 66 / Issue S1 / March 2023
- Published online by Cambridge University Press:
- 19 July 2023, pp. S424-S425
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Introduction
Besides the traditionally studied group of young females, disordered eating occurs in all age groups (Eedena, Hoekena, and Hoek 2021). In recent years, there has been an increase in the prevalence of eating disorders and symptoms in middle-aged and older women (40 years old and over) (Mangweth-Matzek and Hoek 2017).
Experts in eating psychopathology in special groups such as Samuels, Maine and Taltillo (2019) suggest the use of the Screen for Disordered Eating (SDE; Magen et al. 2018) in the psychometric assessment of women in middle age and older. The SDE was developed to allow the Eating Disorders (ED) screening in Primary Health Care in people of all ages and without excluding Binge Eating Disorder (BED).
The SDE is composed of five items (yes or no answers), extracted from other validated self-reported questionnaires for the assessment of eating psychopathology.
ObjectivesTo analyze the psychometric properties of the Portuguese Version of the Screen for Disordered Eating in a sample of women from the general population aged 40 and over.
MethodsParticipants were 516 women with a mean age of 50.28 of years old (± 8.063; range: 40-80). They answered an online survey including the preliminary Portuguese version of the SDE and the Portuguese version of the Eating Disorder Examination – Questionnaire (EDE-Q-7; Pereira et al. 2021).
ResultsConfirmatory Factor Analysis showed that the unidimensional model presented good fit indexes (χ2/df=1.502; RMSEA=.0311, p<.001; CFI=.987 TLI=.995, GFI=.965). The Cronbach’s alfa was .762. All the items contributed to the internal consistency, as they presented item-total correlations above .40 and the exclusion of each one would decrease the alpha. Pearson correlations between SDE and the EDE-Q-7 were significant (p<.01), positive and moderate/high, as follows: .516 with the total score and .318, .503 and .536 respectively with the dimensional scores of Dietary restraint, Shape/weight overvaluation and Body dissatisfaction.
ConclusionsAs observed with the original English-language scale, the Portuguese version of the SDE has shown good validity (construct and concurrent) and internal consistency. As such, the SDE might be a useful tool to investigate disordered eating psychopathology in older women. In the near future we will determine the SDE cut-offs with the best combination of sensitivity and specificity to screen for eating disorders in this populational group.
Disclosure of InterestNone Declared
When the SAINT goes marching in – A novel transcranial magnetic stimulation protocol shows miraculous promise
- J. D. Vieira de Andrade, M. J. Brito
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- Journal:
- European Psychiatry / Volume 66 / Issue S1 / March 2023
- Published online by Cambridge University Press:
- 19 July 2023, p. S835
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Introduction
Transcranial Magnetic Stimulation (TMS) is a non-invasive neuromodulation tool with a growing body of clinical evidence demonstrating positive outcomes in patients with treatment-resistant depression (TRD) as sole or adjuvant therapy. Theta-burst stimulation (TBS), specifically intermittent TBS (iTBS), uses short intermittent pulse trains to cut each session’s duration to 10% of the original repetitive TMS protocol sessions, making it a more appealing option given that it shows similar efficacy. Nevertheless, the number of sessions required remains the same, with a single protocol lasting around 4 to 6 weeks, or longer. However, a new protocol has very recently been approved by the FDA for application in TRD, called the SAINT (Stanford Intelligent Accelerated Neuromodulation Therapy), which reduces treatment duration to 5 days.
ObjectivesTo ascertain what evidence supports the SAINT protocol and its efficacy by reviewing available published literature.
MethodsA PubMed database search was performed and the main findings of selected studies were summarized.
ResultsThree articles were found, which consisted of clinical trials with small study samples of TRD patients. One study found a 90% remission rate after the aforementioned 5-day treatment regimen, with another reporting a 79% response rate after a double-blinded trial. All studies reported no difference in tolerability compared with regular iTBS protocols.
ConclusionsThe SAINT protocol shows promising preliminary results, with efficacy, tolerability and safety of use comparable with that of TMS protocols already in use. The reduction in treatment duration that this intensive option is based on is a significant improvement for applicability in clinical practice, which might increase patient compliance and offer quicker results. Further studies are required to evaluate whether the remission rates are maintained in the long term.
Disclosure of InterestNone Declared
COVID-19 Pandemic: Another Source of Stress for Medical Students
- A.T. Pereira, C. Cabacos, A. Araújo, M.J. Soares, M.J. Brito, F. Carvalho, D. Mota, M. Bajouco, N. Madeira, M. Carneiro, A. Macedo
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- Journal:
- European Psychiatry / Volume 65 / Issue S1 / June 2022
- Published online by Cambridge University Press:
- 01 September 2022, pp. S495-S496
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Introduction
The COVID-19 pandemic has completely changed the experience of higher education with potentially negative consequences for students’ wellbeing.
ObjectivesTo compare medicine/dentistry students’ depression/anxiety/stress levels before versus during the pandemic and to analyse the role of COVID-19-related stressors in their psychological distress.
MethodsStudents from the Faculty of Medicine University of Coimbra answered socio-demographic and personality questionnaires and the Depression, Anxiety and Stress Scale/DASS before (academic years 2016-2017-2018-2019 - SAMPLE1; n=1000) and during (September-December 2020 and January-March 2021 - SAMPLE2; n=650) the COVID-19 pandemic. Mean age (21.12±3.75), personality traits scores, and gender proportions (»75% girls) did not significantly differ between samples. SAMPLE2 also filled in the Fear of COVID-19 Scale and a new version of the Inventory of Sources of Stress During Medical Education/ISSDME, containing a COVID-19 -related dimension (restrictions on training and on socializing with friends/colleagues).
ResultsSAMPLE2 presented significantly higher mean scores of depression (3.89±3.55vs.3.33±3.34), anxiety (3.27±4.08vs.2.86±3.29), stress (7.07±5.72vs.6.18±4.59) and total DASS (12.28±10.55vs.13.65±11.13) than SAMPLE1 (all p<.05). Fear of COVID-19 was a significant predictor of DASS score (adjusted R2=2.9%, p<.001). COVID-19-related stressors continued explaining significant increments of DASS variance after controlling for each of the ISSDME dimensions: Course demands (R2 Change=1.8%), Human demands (2.5%), Lifestyle (2.3%), Academic competition (5.5%), and Academic adjustment (5.2%) (all p<.001).
ConclusionsThis study adds to the evidence of the negative impact of COVID-19 on students and emphasizes its pernicious role on medical students’ psychological distress, which is already higher due to the individual and academic stressors to which they are more exposed.
DisclosureNo significant relationships.
Metabolic changes, agronomic performance, and quality of seeds in soybean with the pat gene after application of glufosinate
- Alfredo Junior P. Albrecht, Ivana Paula F. S. de Brito, Leandro P. Albrecht, André Felipe M. Silva, Ana Karollyna A. de Matos, Caio Antonio Carbonari, Edivaldo D. Velini
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- Journal:
- Weed Science / Volume 68 / Issue 6 / November 2020
- Published online by Cambridge University Press:
- 02 October 2020, pp. 594-604
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The transgenic Liberty Link® (LL) soybean is tolerant to glufosinate, conferred by the enzyme phosphinothricin acetyltransferase (PAT), which is encoded by the pat gene from Streptomyces viridochromogenes. Because symptoms of injury can be observed in soybean [Glycine max (L.) Merr.] plants in some situations, this study evaluated the effects of rates of glufosinate on agronomic performance; quality of LL soybean seeds; and the ammonia, glufosinate, and N-acetyl-l-glufosinate concentration (NAG) in soybeans with and without the pat gene after application of increasing glufosinate rates. Field and greenhouse experiments were conducted; the first evaluated the selectivity of glufosinate in LL soybeans, and the second evaluated the metabolic changes in soybeans with (LL) and without (RR2) the pat gene, after application of glufosinate. For fieldwork, application of glufosinate at rates up to four times the maximum recommended caused initial injury symptoms (up to 38.5%) in LL soybean plants. However, no negative effect was found on seed quality and agronomic performance of LL plants, including yield. This shows the selectivity of glufosinate promoted by pat gene insertion for application in POST (V4), in LL soybean. For the greenhouse experiment, it was concluded that the LL soybean plants presented high glufosinate metabolism, lower ammonia concentration, and no reduction in dry matter, in comparison with RR2 soybean, after application of high rates of glufosinate.
Survival and predictors of deaths of patients hospitalised due to COVID-19 from a retrospective and multicentre cohort study in Brazil
- M. M. Santos, E. E. S. Lucena, K. C. Lima, A. A. C. Brito, M. B. Bay, D. Bonfada
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- Journal:
- Epidemiology & Infection / Volume 148 / 2020
- Published online by Cambridge University Press:
- 07 September 2020, e198
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This study aimed to analyse the survival of patients admitted to Brazilian hospitals due to the COVID-19 and estimate prognostic factors. This is a retrospective, multicentre cohort study, based on data from 46 285 hospitalisations for COVID-19 in Brazil. Survival functions were calculated using the Kaplan–Meier's method. The log-rank test compared the survival functions for each variable and from that, hazard ratios (HRs) were calculated, and the proportional hazard model was used in Cox multiple regression. The smallest survival curves were the ones for patients at the age of 68 years or more, black/mixed race, illiterate, living in the countryside, dyspnoea, respiratory distress, influenza-like outbreak, O2 saturation <95%, X-ray change, length of stay in the intensive care unit (ICU), invasive ventilatory support, previous heart disease, pneumopathy, diabetes, Down's syndrome, neurological disease and kidney disease. Better survival was observed in the influenza-like outbreak and in an asthmatic patient. The multiple model for increased risk of death when they were admitted to the ICU HR 1.28, diabetes HR 1.17, neurological disease HR 1.34, kidney disease HR 1.11, heart disease HR 1.14, black or mixed race of HR 1.50, asthma HR 0.71 and pneumopathy HR 1.12. This reinforces the importance of socio-demographic and clinical factors as a prognosis for death.
Psychopathology and global functioning in schizophrenic patients on depot antypsychotics and long-acting injectable risperidone: A six month comparative study
- A. Intxausti, A.L. Morera, C.C. González-Hernández, D. Alonso-Diaz, N. González-Brito, D. Hernández-García, M. Henry, A. Orozco, E. Díaz-Mesa, E. de Diego-Herrero
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- European Psychiatry / Volume 22 / Issue S1 / March 2007
- Published online by Cambridge University Press:
- 16 April 2020, p. S116
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Introduction:
The introduction of the first atypical antipsychotic with a long acting formulation has open new therapeutic options for the treatment of schizophrenic patients. Our objective consists of comparing psychopathology levels and global functioning in patients with paranoid schizophrenia treated in monotherapy either with long-acting injectable risperidone (LAIR) or conventional depot antipsychotics (DA).
Methods:Patients attending at the community mental health center during the six-month recruitment period were eligible to enter the study. Scores achieved in positive and negative subscales of PANNS and EEAG scale of (Global Activity Evaluating Scale) were evaluated at baseline and 6 months later. Six patients treated with RLAI and six patients treated with DA were recruited. Data were analyzed both with the real sample (N=6 per group) and extrapoling the same results to a bigger sample size (N=24 per group).
Results:Mean increase in scores for both PANNS positive and negative subscales were lower in patients treated with RLAI that in those treated with DA (positive subscale: 0.018±0.06 vs. 0.048±0.03, RLAI and DA, respectively, p=0.387; negative subscale: 0.232±0.076 vs. 0.3095±0.123, RLAI and DA, respectively, p=0.579). EEAG scores were higher for patients treated with RLAI than those treated with DA (1.250±0.56 vs. 0.333±0.225, p=0.144). When these results are extrapolated to a sample of 24 patients per group, differences in EEAG reach statistical significance (p=0.034).
Conclusions:After 6 months of treatment, patients treated with RLAI tend to show a greater improvement in their global activity than those treated with DA.
Isolated perfused udder model for transcriptome analysis in response to Streptococcus agalactiae
- Mayara M. D. C. A. Weller, Isabela Fonseca, Ana P. Sbardella, Isabella S. B. Pinto, Lyderson F. Viccini, Humberto M. Brandão, Juliana C. Gern, Wanessa A. Carvalho, Alessandro S. Guimarães, Maria A. V. P. Brito, Danísio P. Munari, Marcos V. G. B. Silva, Marta F. Martins
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- Journal:
- Journal of Dairy Research / Volume 86 / Issue 3 / August 2019
- Published online by Cambridge University Press:
- 27 August 2019, pp. 307-314
- Print publication:
- August 2019
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This study aimed to evaluate the transcriptional changes occurring in isolated perfused mammary alveolar tissue in response to inoculation with S. agalactiae and to identify the most affected biological functions and pathways after 3 h. Four udders taken at slaughter from cows with healthy mammary gland were perfused ex situ with warmed and gassed Tyrode's solution. Mammary alveolar tissue samples were taken from the left fore and rear quarters (IQ-inoculated quarters) before inoculation (hour 0) and at 3 h post inoculation (hpi) and at the same times from control right fore and rear quarters (not inoculated: NIQ). A total of 1756 differentially expressed genes (DEGs) were identified between IQ and NIQ at 3 hpi using edgeR package. Within this set of DEGs, 952 were up regulated and mainly involved with innate immune response and inflammatory response, e.g., CD14, CCL5, TLR2, IL-8, SAA3, as well as in transcriptional regulation such as FOS, STAT3 and NFKBIA. Genes down-regulated (804) included those involved with lipid synthesis e.g., APOC2, SCD, FABP3 and FABP4. The most affected pathways were chemokine signaling, Wnt signaling and complement and coagulation cascades, which likely reflects the early stage response of mammary tissue to S. agalactiae infection. No significant gene expression changes were detected by RNA-Seq in the others contrasts. Real time-PCR confirmed the increase in mRNA abundance of immune-related genes: TLR2, TLR4, IL-1β, and IL-10 at 3 hpi between IQ and NIQ. The expression profiles of Casp1 and Bax for any contrasts were unaffected whereas Bcl2 was increased in IQ, which suggests no induction of apoptosis during the first hours after infection. Results provided novel information regarding the early functional pathways and gene network that orchestrate innate immune responses to S. agalactiae infection. This knowledge could contribute to new strategies to enhance resistance to this disease, such as genomic selection.
Genotype imputation from various low-density SNP panels and its impact on accuracy of genomic breeding values in pigs
- D. A. Grossi, L. F. Brito, M. Jafarikia, F. S. Schenkel, Z. Feng
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The uptake of genomic selection (GS) by the swine industry is still limited by the costs of genotyping. A feasible alternative to overcome this challenge is to genotype animals using an affordable low-density (LD) single nucleotide polymorphism (SNP) chip panel followed by accurate imputation to a high-density panel. Therefore, the main objective of this study was to screen incremental densities of LD panels in order to systematically identify one that balances the tradeoffs among imputation accuracy, prediction accuracy of genomic estimated breeding values (GEBVs), and genotype density (directly associated with genotyping costs). Genotypes using the Illumina Porcine60K BeadChip were available for 1378 Duroc (DU), 2361 Landrace (LA) and 3192 Yorkshire (YO) pigs. In addition, pseudo-phenotypes (de-regressed estimated breeding values) for five economically important traits were provided for the analysis. The reference population for genotyping imputation consisted of 931 DU, 1631 LA and 2103 YO animals and the remainder individuals were included in the validation population of each breed. A LD panel of 3000 evenly spaced SNPs (LD3K) yielded high imputation accuracy rates: 93.78% (DU), 97.07% (LA) and 97.00% (YO) and high correlations (>0.97) between the predicted GEBVs using the actual 60 K SNP genotypes and the imputed 60 K SNP genotypes for all traits and breeds. The imputation accuracy was influenced by the reference population size as well as the amount of parental genotype information available in the reference population. However, parental genotype information became less important when the LD panel had at least 3000 SNPs. The correlation of the GEBVs directly increased with an increase in imputation accuracy. When genotype information for both parents was available, a panel of 300 SNPs (imputed to 60 K) yielded GEBV predictions highly correlated (⩾0.90) with genomic predictions obtained based on the true 60 K panel, for all traits and breeds. For a small reference population size with no parents on reference population, it is recommended the use of a panel at least as dense as the LD3K and, when there are two parents in the reference population, a panel as small as the LD300 might be a feasible option. These findings are of great importance for the development of LD panels for swine in order to reduce genotyping costs, increase the uptake of GS and, therefore, optimize the profitability of the swine industry.
Variation in the Sensitivities of Hairy Beggarticks (Bidens pilosa) Plants and Their Progenies to Glufosinate Ammonium
- Ivana P. F. S. de Brito, Bruna B. Marchesi, Carolina Pucci, Caio A. Carbonari, Edivaldo D. Velini
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- Weed Science / Volume 64 / Issue 4 / December 2016
- Published online by Cambridge University Press:
- 20 January 2017, pp. 570-578
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The objective of this study was to evaluate the response of hairy beggarticks plants to different doses of glufosinate ammonium and the range in sensitivities of the plants and their progenies to the herbicide. Three studies were conducted, all in a greenhouse and repeated at different times. In the first study, two experiments were conducted to examine the dose–response curve, and the treatments were seven different doses of the herbicide glufosinate ammonium (0, 50, 100, 200, 400, 800, and 1,600 g ai ha−1), with four replications each. In the second study, which examined the range in sensitivity of hairy beggarticks to glufosinate ammonium, 44 plants were sprayed with a dose of 200 g ai ha−1 of the herbicide. Finally, in the third study, the range in sensitivity of the progeny of hairy beggarticks to glufosinate ammonium was investigated; in this experiment, the progenies of seven of the previous plants were sprayed with 200 g ai ha−1 of herbicide. The ammonium contents in the tissues were measured and percent injury wase visually assessed. Ammoniun content in hairy beggarticks leaves was increased more than seven times by glufosinate application and the maximum ammonium content was observed for the highest dose of the herbicide. Variability existed in the ammonium content among the individuals of the population of hairy beggarticks; however, the behavior was not replicated in the same way in the progenies. The survival of the plants after application of the herbicide allows the production of progenies with wide variability in their sensitivity to the product, independent of the behavior for the progenitor plants.
Avian pathogenic Escherichia coli - methods for improved diagnosis
- H.C. KUNERT FILHO, D. CARVALHO, T.T. GRASSOTTI, B.D. SOARES, J.M. ROSSATO, A.C. CUNHA, K.C.T. BRITO, L.S. CAVALLI, B.G. BRITO
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- Journal:
- World's Poultry Science Journal / Volume 71 / Issue 2 / June 2015
- Published online by Cambridge University Press:
- 03 June 2015, pp. 249-258
- Print publication:
- June 2015
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Avian pathogenic Escherichia coli (APEC) cause extra intestinal disease in chickens, turkeys, and other avian species via respiratory tract infection, giving rise to typical signs related to colibacillosis, such as: septicaemia, enteritis, granulomas, omphalitis, sinusitis, airsacculitis, arthritis/synovitis, peritonitis, pericarditis, perihepatitis, cellulitis, and swollen head syndrome. Disease caused by APEC strains results in significant financial losses to the poultry industry worldwide. This review provides essential information on the diagnosis of APEC. The information cited in this review will elucidate the various forms of APEC diagnosis while demonstrating the, lack of a precise diagnosis of this strain. The diagnoses mentioned herein reinforce the necessity to make progress toward a more accurate diagnosis of APEC to contribute to its reduction in poultry flocks.
Review of the distribution and conservation status of the terrestrial reptiles of the Cape Verde Islands
- Raquel Vasconcelos, José Carlos Brito, Salvador Carranza, D. James Harris
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Cape Verde has a higher number of reptile taxa and endemics than any of the five archipelagos in the Macaronesian region. Mapping the precise distributions and assessing the conservation status of reptiles is the first step towards effective conservation. Presence/absence and abundance data were gathered from extensive fieldwork and post-1980 literature. Evaluation of conservation status was considered at specific and subspecific levels, following IUCN Red List criteria and using RAMAS. Fieldwork confirmed the occurrence of 34 of 37 previously recorded taxa (31 native, three exotic). One taxon continues to be considered Extinct. Three broad distribution and rarity patterns were identified: widespread and abundant taxa occurring on ≥ 2 islands/islets, widespread or abundant taxa restricted to one island, and rare or limited range taxa occurring on small areas of islands or islets. More than a third of taxa have areas of occupancy < 20 km2 and extents of occurrence < 100 km2. Geckos are rarer than skinks because of their high habitat specialization, with 58% occurring on only one island/islet. About half of all taxa are potentially threatened, twice the proportion of those in the Canary Islands, a difference that could be explained by the smaller area and greater aridity of the Cape Verde islands. The criterion used for most threat categorizations is geographical range, and the most pervasive threats are natural disasters, intrinsic factors of the species and introduced species. The importance of applying conservation status at the subspecific level to island endemics is emphasized. Several conservation measures are proposed, including optimized design of protected areas.
Findings of the International Nosocomial Infection Control Consortium (INICC), Part I: Effectiveness of a Multidimensional Infection Control Approach on Catheter-Associated Urinary Tract Infection Rates in Pediatric Intensive Care Units of 6 Developing Countries
- Victor D. Rosenthal, Bala Ramachandran, Lourdes Dueñas, Carlos Álvarez-Moreno, J. A. Navoa-Ng, Alberto Armas-Ruiz, Gulden Ersoz, Lorena Matta-Cortés, Mandakini Pawar, Ata Nevzat-Yalcin, Marena Rodriguez-Ferrer, Ana Concepción Bran de Casares, Claudia Linares, Victoria D. Villanueva, Roberto Campuzano, Ali Kaya, Luis Fernando Rendon-Campo, Amit Gupta, Ozge Turhan, Nayide Barahona-Guzmán, Lilian de Jesús-Machuca, María Corazon V. Tolentino, Jorge Mena-Brito, Necdet Kuyucu, Yamileth Astudillo, Narinder Saini, Nurgul Gunay, Guillermo Sarmiento-Villa, Eylul Gumus, Alfredo Lagares-Guzmán, Oguz Dursun
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- Journal:
- Infection Control & Hospital Epidemiology / Volume 33 / Issue 7 / July 2012
- Published online by Cambridge University Press:
- 02 January 2015, pp. 696-703
- Print publication:
- July 2012
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Design.
A before-after prospective surveillance study to assess the impact of a multidimensional infection control approach for the reduction of catheter-associated urinary tract infection (CAUTI) rates.
Setting.Pediatric intensive care units (PICUs) of hospital members of the International Nosocomial Infection Control Consortium (INICC) from 10 cities of the following 6 developing countries: Colombia, El Salvador, India, Mexico, Philippines, and Turkey.
Patients.PICU inpatients.
Methods.We performed a prospective active surveillance to determine rates of CAUTI among 3,877 patients hospitalized in 10 PICUs for a total of 27,345 bed-days. The study was divided into a baseline period (phase 1) and an intervention period (phase 2). In phase 1, surveillance was performed without the implementation of the multidimensional approach. In phase 2, we implemented a multidimensional infection control approach that included outcome surveillance, process surveillance, feedback on CAUTI rates, feedback on performance, education, and a bundle of preventive measures. The rates of CAUTI obtained in phase 1 were compared with the rates obtained in phase 2, after interventions were implemented.
Results.During the study period, we recorded 8,513 urinary catheter (UC) days, including 1,513 UC-days in phase 1 and 7,000 UC-days in phase 2. In phase 1, the CAUTI rate was 5.9 cases per 1,000 UC-days, and in phase 2, after implementing the multidimensional infection control approach for CAUTI prevention, the rate of CAUTI decreased to 2.6 cases per 1,000 UC-days (relative risk, 0.43 [95% confidence interval, 0.21–1.0]), indicating a rate reduction of 57%.
Conclusions.Our findings demonstrated that implementing a multidimensional infection control approach is associated with a significant reduction in the CAUTI rate of PICUs in developing countries.
Contributors
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- By Rose Teteki Abbey, K. C. Abraham, David Tuesday Adamo, LeRoy H. Aden, Efrain Agosto, Victor Aguilan, Gillian T. W. Ahlgren, Charanjit Kaur AjitSingh, Dorothy B E A Akoto, Giuseppe Alberigo, Daniel E. Albrecht, Ruth Albrecht, Daniel O. Aleshire, Urs Altermatt, Anand Amaladass, Michael Amaladoss, James N. Amanze, Lesley G. Anderson, Thomas C. Anderson, Victor Anderson, Hope S. Antone, María Pilar Aquino, Paula Arai, Victorio Araya Guillén, S. Wesley Ariarajah, Ellen T. Armour, Brett Gregory Armstrong, Atsuhiro Asano, Naim Stifan Ateek, Mahmoud Ayoub, John Alembillah Azumah, Mercedes L. García Bachmann, Irena Backus, J. Wayne Baker, Mieke Bal, Lewis V. Baldwin, William Barbieri, António Barbosa da Silva, David Basinger, Bolaji Olukemi Bateye, Oswald Bayer, Daniel H. Bays, Rosalie Beck, Nancy Elizabeth Bedford, Guy-Thomas Bedouelle, Chorbishop Seely Beggiani, Wolfgang Behringer, Christopher M. Bellitto, Byard Bennett, Harold V. Bennett, Teresa Berger, Miguel A. Bernad, Henley Bernard, Alan E. Bernstein, Jon L. Berquist, Johannes Beutler, Ana María Bidegain, Matthew P. Binkewicz, Jennifer Bird, Joseph Blenkinsopp, Dmytro Bondarenko, Paulo Bonfatti, Riet en Pim Bons-Storm, Jessica A. Boon, Marcus J. Borg, Mark Bosco, Peter C. Bouteneff, François Bovon, William D. Bowman, Paul S. Boyer, David Brakke, Richard E. Brantley, Marcus Braybrooke, Ian Breward, Ênio José da Costa Brito, Jewel Spears Brooker, Johannes Brosseder, Nicholas Canfield Read Brown, Robert F. Brown, Pamela K. Brubaker, Walter Brueggemann, Bishop Colin O. Buchanan, Stanley M. Burgess, Amy Nelson Burnett, J. Patout Burns, David B. Burrell, David Buttrick, James P. Byrd, Lavinia Byrne, Gerado Caetano, Marcos Caldas, Alkiviadis Calivas, William J. Callahan, Salvatore Calomino, Euan K. Cameron, William S. Campbell, Marcelo Ayres Camurça, Daniel F. Caner, Paul E. Capetz, Carlos F. Cardoza-Orlandi, Patrick W. Carey, Barbara Carvill, Hal Cauthron, Subhadra Mitra Channa, Mark D. Chapman, James H. Charlesworth, Kenneth R. Chase, Chen Zemin, Luciano Chianeque, Philip Chia Phin Yin, Francisca H. Chimhanda, Daniel Chiquete, John T. Chirban, Soobin Choi, Robert Choquette, Mita Choudhury, Gerald Christianson, John Chryssavgis, Sejong Chun, Esther Chung-Kim, Charles M. A. Clark, Elizabeth A. Clark, Sathianathan Clarke, Fred Cloud, John B. Cobb, W. Owen Cole, John A Coleman, John J. Collins, Sylvia Collins-Mayo, Paul K. Conkin, Beth A. Conklin, Sean Connolly, Demetrios J. Constantelos, Michael A. Conway, Paula M. Cooey, Austin Cooper, Michael L. Cooper-White, Pamela Cooper-White, L. William Countryman, Sérgio Coutinho, Pamela Couture, Shannon Craigo-Snell, James L. Crenshaw, David Crowner, Humberto Horacio Cucchetti, Lawrence S. Cunningham, Elizabeth Mason Currier, Emmanuel Cutrone, Mary L. Daniel, David D. Daniels, Robert Darden, Rolf Darge, Isaiah Dau, Jeffry C. Davis, Jane Dawson, Valentin Dedji, John W. de Gruchy, Paul DeHart, Wendy J. Deichmann Edwards, Miguel A. De La Torre, George E. Demacopoulos, Thomas de Mayo, Leah DeVun, Beatriz de Vasconcellos Dias, Dennis C. Dickerson, John M. Dillon, Luis Miguel Donatello, Igor Dorfmann-Lazarev, Susanna Drake, Jonathan A. Draper, N. Dreher Martin, Otto Dreydoppel, Angelyn Dries, A. J. Droge, Francis X. D'Sa, Marilyn Dunn, Nicole Wilkinson Duran, Rifaat Ebied, Mark J. Edwards, William H. Edwards, Leonard H. Ehrlich, Nancy L. Eiesland, Martin Elbel, J. Harold Ellens, Stephen Ellingson, Marvin M. Ellison, Robert Ellsberg, Jean Bethke Elshtain, Eldon Jay Epp, Peter C. Erb, Tassilo Erhardt, Maria Erling, Noel Leo Erskine, Gillian R. Evans, Virginia Fabella, Michael A. Fahey, Edward Farley, Margaret A. Farley, Wendy Farley, Robert Fastiggi, Seena Fazel, Duncan S. Ferguson, Helwar Figueroa, Paul Corby Finney, Kyriaki Karidoyanes FitzGerald, Thomas E. FitzGerald, John R. Fitzmier, Marie Therese Flanagan, Sabina Flanagan, Claude Flipo, Ronald B. Flowers, Carole Fontaine, David Ford, Mary Ford, Stephanie A. Ford, Jim Forest, William Franke, Robert M. Franklin, Ruth Franzén, Edward H. Friedman, Samuel Frouisou, Lorelei F. Fuchs, Jojo M. Fung, Inger Furseth, Richard R. Gaillardetz, Brandon Gallaher, China Galland, Mark Galli, Ismael García, Tharscisse Gatwa, Jean-Marie Gaudeul, Luis María Gavilanes del Castillo, Pavel L. Gavrilyuk, Volney P. Gay, Metropolitan Athanasios Geevargis, Kondothra M. George, Mary Gerhart, Simon Gikandi, Maurice Gilbert, Michael J. Gillgannon, Verónica Giménez Beliveau, Terryl Givens, Beth Glazier-McDonald, Philip Gleason, Menghun Goh, Brian Golding, Bishop Hilario M. Gomez, Michelle A. Gonzalez, Donald K. Gorrell, Roy Gottfried, Tamara Grdzelidze, Joel B. Green, Niels Henrik Gregersen, Cristina Grenholm, Herbert Griffiths, Eric W. Gritsch, Erich S. Gruen, Christoffer H. Grundmann, Paul H. Gundani, Jon P. Gunnemann, Petre Guran, Vidar L. Haanes, Jeremiah M. Hackett, Getatchew Haile, Douglas John Hall, Nicholas Hammond, Daphne Hampson, Jehu J. Hanciles, Barry Hankins, Jennifer Haraguchi, Stanley S. Harakas, Anthony John Harding, Conrad L. Harkins, J. William Harmless, Marjory Harper, Amir Harrak, Joel F. Harrington, Mark W. Harris, Susan Ashbrook Harvey, Van A. Harvey, R. Chris Hassel, Jione Havea, Daniel Hawk, Diana L. Hayes, Leslie Hayes, Priscilla Hayner, S. Mark Heim, Simo Heininen, Richard P. Heitzenrater, Eila Helander, David Hempton, Scott H. Hendrix, Jan-Olav Henriksen, Gina Hens-Piazza, Carter Heyward, Nicholas J. Higham, David Hilliard, Norman A. Hjelm, Peter C. Hodgson, Arthur Holder, M. Jan Holton, Dwight N. Hopkins, Ronnie Po-chia Hsia, Po-Ho Huang, James Hudnut-Beumler, Jennifer S. Hughes, Leonard M. Hummel, Mary E. Hunt, Laennec Hurbon, Mark Hutchinson, Susan E. Hylen, Mary Beth Ingham, H. Larry Ingle, Dale T. Irvin, Jon Isaak, Paul John Isaak, Ada María Isasi-Díaz, Hans Raun Iversen, Margaret C. Jacob, Arthur James, Maria Jansdotter-Samuelsson, David Jasper, Werner G. Jeanrond, Renée Jeffery, David Lyle Jeffrey, Theodore W. Jennings, David H. Jensen, Robin Margaret Jensen, David Jobling, Dale A. Johnson, Elizabeth A. Johnson, Maxwell E. Johnson, Sarah Johnson, Mark D. Johnston, F. Stanley Jones, James William Jones, John R. Jones, Alissa Jones Nelson, Inge Jonsson, Jan Joosten, Elizabeth Judd, Mulambya Peggy Kabonde, Robert Kaggwa, Sylvester Kahakwa, Isaac Kalimi, Ogbu U. Kalu, Eunice Kamaara, Wayne C. Kannaday, Musimbi Kanyoro, Veli-Matti Kärkkäinen, Frank Kaufmann, Léon Nguapitshi Kayongo, Richard Kearney, Alice A. Keefe, Ralph Keen, Catherine Keller, Anthony J. Kelly, Karen Kennelly, Kathi Lynn Kern, Fergus Kerr, Edward Kessler, George Kilcourse, Heup Young Kim, Kim Sung-Hae, Kim Yong-Bock, Kim Yung Suk, Richard King, Thomas M. King, Robert M. Kingdon, Ross Kinsler, Hans G. Kippenberg, Cheryl A. Kirk-Duggan, Clifton Kirkpatrick, Leonid Kishkovsky, Nadieszda Kizenko, Jeffrey Klaiber, Hans-Josef Klauck, Sidney Knight, Samuel Kobia, Robert Kolb, Karla Ann Koll, Heikki Kotila, Donald Kraybill, Philip D. W. Krey, Yves Krumenacker, Jeffrey Kah-Jin Kuan, Simanga R. Kumalo, Peter Kuzmic, Simon Shui-Man Kwan, Kwok Pui-lan, André LaCocque, Stephen E. Lahey, John Tsz Pang Lai, Emiel Lamberts, Armando Lampe, Craig Lampe, Beverly J. Lanzetta, Eve LaPlante, Lizette Larson-Miller, Ariel Bybee Laughton, Leonard Lawlor, Bentley Layton, Robin A. Leaver, Karen Lebacqz, Archie Chi Chung Lee, Marilyn J. Legge, Hervé LeGrand, D. L. LeMahieu, Raymond Lemieux, Bill J. Leonard, Ellen M. Leonard, Outi Leppä, Jean Lesaulnier, Nantawan Boonprasat Lewis, Henrietta Leyser, Alexei Lidov, Bernard Lightman, Paul Chang-Ha Lim, Carter Lindberg, Mark R. Lindsay, James R. Linville, James C. Livingston, Ann Loades, David Loades, Jean-Claude Loba-Mkole, Lo Lung Kwong, Wati Longchar, Eleazar López, David W. Lotz, Andrew Louth, Robin W. Lovin, William Luis, Frank D. Macchia, Diarmaid N. J. MacCulloch, Kirk R. MacGregor, Marjory A. MacLean, Donald MacLeod, Tomas S. Maddela, Inge Mager, Laurenti Magesa, David G. Maillu, Fortunato Mallimaci, Philip Mamalakis, Kä Mana, Ukachukwu Chris Manus, Herbert Robinson Marbury, Reuel Norman Marigza, Jacqueline Mariña, Antti Marjanen, Luiz C. L. Marques, Madipoane Masenya (ngwan'a Mphahlele), Caleb J. D. Maskell, Steve Mason, Thomas Massaro, Fernando Matamoros Ponce, András Máté-Tóth, Odair Pedroso Mateus, Dinis Matsolo, Fumitaka Matsuoka, John D'Arcy May, Yelena Mazour-Matusevich, Theodore Mbazumutima, John S. McClure, Christian McConnell, Lee Martin McDonald, Gary B. McGee, Thomas McGowan, Alister E. McGrath, Richard J. McGregor, John A. McGuckin, Maud Burnett McInerney, Elsie Anne McKee, Mary B. McKinley, James F. McMillan, Ernan McMullin, Kathleen E. McVey, M. 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Yee, Viktor Yelensky, Yeo Khiok-Khng, Gustav K. K. Yeung, Angela Yiu, Amos Yong, Yong Ting Jin, You Bin, Youhanna Nessim Youssef, Eliana Yunes, Robert Michael Zaller, Valarie H. Ziegler, Barbara Brown Zikmund, Joyce Ann Zimmerman, Aurora Zlotnik, Zhuo Xinping
- Edited by Daniel Patte, Vanderbilt University, Tennessee
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- The Cambridge Dictionary of Christianity
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- 05 August 2012
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- 20 September 2010, pp xi-xliv
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Photostability of gas- and solid-phase biomolecules under astrophysical analog soft X-rays field
- S. Pilling, D. P. P. Andrade, R. T. Marinho, E. M. do Nascimento, H. M. Boechat-Roberty, R. B. de Castilho, G. G. B. de Souza, L. H. Coutinho, R. L. Cavasso-Filho, A. F. Lago, A. N. de Brito
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- Journal:
- Proceedings of the International Astronomical Union / Volume 5 / Issue S265 / August 2009
- Published online by Cambridge University Press:
- 09 March 2010, pp. 440-441
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- August 2009
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We present experimental studies on the interaction of soft X-rays on gas-phase and solid-phase amino acids and nucleobases in an attempt to verify if these molecules (supposed to be formed in molecular clouds/protostellar clouds) can survive long enough to be observed or even to be found in meteorites. Measurements have been undertaken employing 150 eV photons under high vacuum conditions at the Brazilian Synchrotron Light Laboratory (LNLS). The produced ions from the gas-phase experiments (glycine, adenine and uracil) have been mass/charge analyzed by time-of-flight spectrometer. The analysis of solid phase samples (glycine, DL-proline, DL-valine, adenine and uracil) were performed by a Fourier transform infrared spectrometer coupled to the experimental chamber. Photodissociation cross sections and halflives were determined and extrapolated to astrophysical environments. The nucleobases photostability was up to two orders of magnitude higher than for the amino acids.
Rotating spherical Couette flow in a dipolar magnetic field: experimental study of magneto-inertial waves
- DENYS SCHMITT, T. ALBOUSSIÈRE, D. BRITO, P. CARDIN, N. GAGNIÈRE, D. JAULT, H.-C. NATAF
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- Journal of Fluid Mechanics / Volume 604 / 10 June 2008
- Published online by Cambridge University Press:
- 14 May 2008, pp. 175-197
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The magnetostrophic regime, in which Lorentz and Coriolis forces are in balance, has been investigated in a rapidly rotating spherical Couette flow experiment. The spherical shell is filled with liquid sodium and permeated by a strong imposed dipolar magnetic field. Azimuthally travelling hydromagnetic waves have been put in evidence through a detailed analysis of electric potential differences measured on the outer sphere, and their properties have been determined. Several types of wave have been identified depending on the relative rotation rates of the inner and outer spheres: they differ by their dispersion relation and by their selection of azimuthal wavenumbers. In addition, these waves constitute the largest contribution to the observed fluctuations, and all of them travel in the retrograde direction in the frame of reference bound to the fluid. We identify these waves as magneto-inertial waves by virtue of the close proximity of the magnetic and inertial characteristic time scales of relevance in our experiment.
Survival of gas phase amino acids and nucleobases in space radiation conditions
- S. Pilling, D. P. P. Andrade, R. B. de Castilho, R. L. Cavasso-Filho, A. F. Lago, L. H. Coutinho, G. G. B. de Souza, H. M. Boechat-Roberty, A. Naves de Brito
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- Journal:
- Proceedings of the International Astronomical Union / Volume 4 / Issue S251 / February 2008
- Published online by Cambridge University Press:
- 01 February 2008, pp. 371-376
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- February 2008
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We present experimental studies on the photoionization and photodissociation processes (photodestruction) of gaseous amino acids and nucleobases in interstellar and interpla-netary radiation analogs conditions. The measurements have been undertaken at the Brazilian Synchrotron Light Laboratory (LNLS), employing vacuum ultraviolet (VUV) and soft X-ray photons. The experimental set up basically consists of a time-of-flight mass spectrometer kept under high vacuum conditions. Mass spectra were obtained using a photoelectron photoion coincidence technique. We have shown that the amino acids are effectively more destroyed (up to 70–80%) by the stellar radiation than the nucleobases, mainly in the VUV. Since polycyclic aromatic hydrocarbons have the same survival capability and seem to be ubiquitous in the ISM, it is not unreasonable to predict that nucleobases could survive in the interstellar medium and/or in comets, even as a stable cation.
Experimental and numerical studies of magnetoconvection in a rapidly rotating spherical shell
- N. GILLET, D. BRITO, D. JAULT, H. C. NATAF
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- Journal of Fluid Mechanics / Volume 580 / 10 June 2007
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- 21 May 2007, pp. 123-143
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Thermal magnetoconvection in a rapidly rotating spherical shell is investigated numerically and experimentally in electrically conductive liquid gallium (Prandtl number P = 0.025), at Rayleigh numbers R up to around 6 times critical and at Ekman numbers E ∼ 10−6. This work follows up the non-magnetic study of convection presented in a companion paper (Gillet et al. 2007). We study here the addition of a z-invariant toroidal magnetic field to the fluid flow. The experimental measurements of fluid velocities by ultrasonic Doppler velocimetry, together with the quasi-geostrophic numerical simulations incorporating a three-dimensional modelling of the magnetic induction processes, demonstrate a stabilizing effect of the magnetic field in the weak-field case, characterized by an Elsasser number Λ < (E/P)1/3. We find that this is explained by the changes of the critical parameters at the onset of convection as Λ increases. As in the non-magnetic study, strong zonal jets of characteristic length scales ℓβ (Rhines length scale) dominates the fluid dynamics. A new characteristic of the magnetoconvective flow is the elongation of the convective cells in the direction of the imposed magnetic field, introducing a new length scale ℓφ. Combining experimental and numerical results, we derive a scaling law where U is the axisymmetric motion amplitude, Ũs and Ũφ are the non-axisymmetric radial and azimuthal motion amplitudes, respectively.
Experimental and numerical studies of convection in a rapidly rotating spherical shell
- N. GILLET, D. BRITO, D. JAULT, H. C. NATAF
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- Journal of Fluid Mechanics / Volume 580 / 10 June 2007
- Published online by Cambridge University Press:
- 21 May 2007, pp. 83-121
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Thermal convection in a rapidly rotating spherical shell is investigated experimentally and numerically. The experiments are performed in water (Prandtl number P=7) and in gallium (P=0.025), at Rayleigh numbers R up to 80 times the critical value in water (up to 6 times critical in gallium) and at Ekman numbers E∼10−6. The measurements of fluid velocities by ultrasonic Doppler velocimetry are quantitatively compared with quasi-geostrophic numerical simulations incorporating a varying β-effect and boundary friction (Ekman pumping). In water, unsteady multiple zonal jets, weaker in amplitude than the non-axisymmetric flow, are experimentally observed and numerically reproduced at moderate forcings (R/Rc<40). In this regime, zonal flows and vortices share the same length scale. Gallium experiments and strongly supercritical convection experiments in water correspond to another regime. In these turbulent flows, the zonal motion amplitude U dominates the non-axisymmetric motion amplitude Ũ. As a result of the reverse cascade of kinetic energy, the characteristic Rhines length scale of zonal jets emerges, and the boundary friction becomes the main brake on the growth of the zonal flow. A scaling law U ∼ Ũ4/3 is then derived and verified both numerically and experimentally.