Exposure to adversity during the perinatal period has been associated with cognitive difficulties in children. Given the role of the nucleus accumbens (NAcc) in attention and impulsivity, we examined whether NAcc volume at age six mediates the relations between pre- and postnatal adversity and subsequent attention problems in offspring. 306 pregnant women were recruited as part of the Growing Up in Singapore Towards Healthy Outcomes Study. Psychosocial stress was assessed during pregnancy and across the first 5 years postpartum. At six years of age, children underwent structural MRI and, at age seven years, mothers reported on their children’s attention problems. Separate factor analyses conducted on measures of pre- and postnatal adversity each yielded two latent factors: maternal mental health and socioeconomic status. Both pre- and postnatal maternal mental health predicted children’s attention difficulties. Further, NAcc volume mediated the relation between prenatal, but not postnatal, maternal mental health and children’s attention problems. These findings suggest that the NAcc is particularly vulnerable to prenatal maternal mental health challenges and contributes to offspring attention problems. Characterizing the temporal sensitivity of neurobiological structures to adversity will help to elucidate mechanisms linking environmental exposures and behavior, facilitating the development of neuroscience-informed interventions for childhood difficulties.

Objectives/Goals: Dravet syndrome is a developmental and epileptic encephalopathy associated with refractory seizures and a high risk of sudden unexpected death in epilepsy. A pathogenic biallelic variant in SCN1B, SCN1B-p.R98C, was identified in three patients with Dravet syndrome. Here we investigate SCN1B-p.R98C on neuronal function in vivo. Methods/Study Population: Scn1b-p.R98C mice were previously generated using CRISPR-Cas9 gene editing. Homozygous animals exhibit increased susceptibility to hyperthermia induced seizures at postnatal day (P) 15, 100% expression of spontaneous generalized seizures by P30, and ~20% undergo SUDEP by approximately P60. Here we examined the neuronal phenotype of P17–28 male and female Scn1b-p.R89C mice. We used whole-cell patch clamp electrophysiology approaches to measure effects of the variant on passive membrane properties, intrinsic excitability, and single action potential properties of parvalbumin positive (PV+) interneurons and pyramidal neurons in layers 5/6 of the somatosensory cortex and CA1 region of the hippocampus. Wild-type littermates were used as controls. Results/Anticipated Results: Our results show no differences between genotypes in any measure for somatosensory cortical PV+ interneurons or pyramidal neurons. In the CA1 region of the hippocampus, we found no differences for any measure in PV+ interneurons. In contrast, CA1 pyramidal neurons were hyperexcitable, however, with no changes in passive membrane properties or single action potential properties. Discussion/Significance of Impact:

Temporary changes in biological state, such as hunger, can impact decision making differently for men and women. Food scarcity is correlated with a host of negative economic outcomes. Two explanations for this correlation are that hunger affects economic preferences directly or that hunger creates a mindset that focuses on scarcity management to the detriment of other decisions. To test these predictions, we conduct a lab-in-the-field experiment in a health screening clinic in Shanghai, recruiting participants who finish their annual physical exam either before or after they have eaten breakfast. We compare the hungry and sated groups on their risk, time and generosity preferences as well as their cognitive performance. Our results show that men and women respond to hunger in opposite directions, thus hunger reduces the gender gap in decision quality, risk aversion and cognitive performance, but creates one in generosity. Finally, we examine several biomarkers and find that higher blood lipid levels are correlated with greater choice inconsistency, risk aversion and generosity. We contribute to emerging insights on the biological foundations for economic preferences and outcomes.

For binary plug nozzle, the plug cone is exposed to high-temperature mainstream flow, making it one of the nozzle’s high-temperature components. This paper uses the Realizable k-ε turbulence model and the reverse Monte Carlo method to numerically investigate the aerodynamic and infrared radiation characteristics of the plug nozzle. Various slot cooling configurations were adopted to study the nozzle’s infrared radiation in detail. Results indicate that compared to the baseline nozzle, the plug nozzle’s performance is slightly reduced due to the decrease in effective area of flow over the plug cone. Introducing slot cooling at the rear edge provides significant infrared suppression benefits at low detection angles and notably reduces infrared radiation discrepancy with baseline nozzle at high detection angles. The cooling air from slots causes the nozzle jet to exhibit a ‘thermal layered’ feature. With the same total coolant mass flow, the ‘leading edge + trailing edge’ cooling configuration can lower the area-averaged wall temperature of the plug cone by 5.5% – 12.3%. However, its infrared radiation intensity at each detection angle on the pitch detection plane is higher than that of the ‘trailing edge’ configuration. The significance of leading-edge cooling is focused more on thermal protection for the plug. Thus, it is essential to balance coolant mass flow distribution between infrared radiation suppression and thermal protection.

Species of the genus Corynosoma (Acanthocephala: Polymorphida) mainly parasitize marine mammals and rarely marine birds, and are of veterinary and medical importance due to causing corynosomiasis in wildlife and humans. However, the current knowledge of the mitochondrial genomes and mitogenomic phylogeny of this group remains very insufficient. In the present study, the complete mitochondrial genomes of C. bullosum (von Linstow, 1892) and C. evae Zdzitowiecki, 1984 were sequenced and annotated for the first time. Both mitogenomes comprise 12 protein-coding genes (missing atp8), 22 tRNA genes, and 2 ribosomal RNAs (rrnS and rrnL), plus 2 non-coding regions (NCR1 and NCR2). Corynosoma bullosum has the largest mitogenome (14,879 bp) of any polymorphid species reported so far, while C. evae has the smallest (13,947 bp), except for Sphaerirostris lanceoides (Petrochenko, 1949). Comparative mitogenomic analysis also revealed the presence of distinct discrepancies in A + T content and gene rearrangement across the families Polymorphidae, Centrorhynchidae, and Plagiorhynchidae. Moreover, phylogenetic analyses based on the concatenated amino acid sequences of 12 protein-coding genes strongly supported the monophyly of the order Polymorphida and a close affinity between the families Polymorphidae and Centrorhynchidae in Polymorphida. The present mitogenomic phylogeny provides additional evidence for a sister relationship between the genera Corynosoma and Bolbosoma and demonstrated that C. evae has a closer relationship with C. villosum than C. bullosum in the genus Corynosoma.

The strategy method (SM) is, in practice, subject to a possibly severe economic-theoretical bias. Although many studies utilize SM to examine responses to rare or off-equilibrium behaviors unattainable through direct elicitation (DE), they ignore the fact that the strategic equivalence between SM and DE holds for the monetary payoff game but not the game participants actually play, which is in terms of utilities. We report three results. First, failing to account for estimation bias when decisions at one information set can influence utility at another may result in significant differences in decision-making. Second, the magnitude of this bias can be substantial, comparable to other measured treatment effects. Third, minor interventions targeting salience can amplify these differences similarly, causing treatment effects to differ significantly between SM and DE, even reversing in direction. These findings emphasize the need for reconsideration of the SM’s reliability for economic research.

This article highlights a potential and significant economic–theoretical bias in the widely used strategy method (SM) technique. Although SM is commonly employed to analyze numerous observations per subject regarding rare or off-equilibrium behaviors unattainable through direct elicitation (DE), researchers often overlook a critical distinction. The strategic equivalence between SM and DE is applicable in the context of monetary payoff games, but not in the actual utility-based games played by participants. This oversight may lead to inaccurate conclusions and demand a reevaluation of existing research in the field. We formalize the mapping from the monetary payoff game to this actual game and delineate necessary and sufficient conditions for strategic equivalence to apply.

The axisymmetric nozzle mechanism is the core part for thrust vectoring of aero engine, which contains complex rigid-flexible coupled multibody system with joints clearance and significantly reduces the efficiency in modeling and calculation, therefore the kinematics and dynamics analysis of axisymmetric vectoring nozzle mechanism based on deep neural network is proposed. The deep neural network model of the axisymmetric vector nozzle is established according to the limited training data from the physical dynamic model and then used to predict the kinematics and dynamics response of the axisymmetric vector nozzle. This study analyses the effects of joint clearance on the kinematics and dynamics of the axisymmetric vector nozzle mechanism by a data-driven model. It is found that the angular acceleration of the expanding blade and the driving force are mostly affected by joint clearance followed by the angle, angular velocity and position of the expanding blade. Larger joint clearance results in more pronounced fluctuations of the dynamic response of the mechanism, which is due to the greater relative velocity and contact force between the bushing and the pin. Since axisymmetric vector nozzles are highly complex nonlinear systems, traditional numerical methods of dynamics are extremely time-consuming. Our work indicates that the data-driven approach greatly reduces the computational cost while maintaining accuracy, and can be used for rapid evaluation and iterative computation of complex multibody dynamics of engine nozzle mechanism.

The high-altitude balloon proposed in this paper is a long-life balloon carrying a payload through a cable that flies at 20km altitude in near space. A dynamic model of the system, including the thermodynamics of the buoyancy body coupled with a hanging model of the pod, is developed using the Newton–Euler method. The buoyancy body consists of a helium balloon and a ballonet. A differential pressure difference-based altitude adjustment is achieved by tracking the pressure difference at the target altitude. A dynamic simulation of the buoyancy body with a slung pod in autonomous vertical takeoff and altitude regulation processes is presented. The internal thermodynamic variations and pressure differential of the buoyancy body are given. The air mass exchange and blower flow control of the ballonet are validated. The altitude holding error is analysed. The maximum pull force that the cable can withstand is calculated, and the maximum attitude angles of the pod during the ascending and descending processes are depicted. Simulation results provide basic knowledge for the structural design and payload installation of pods.

We investigated whether an observed reduction in overall childhood cancer risk (<15 years of age) in twins has been sustained, and how this extends into young adulthood. We searched for English language publications reporting childhood cancer risk in twins, obtained unpublished data directly from some authors, and updated a meta-analysis. We used the Swedish Multigeneration Register to investigate the age to which the reduced overall risk of childhood cancer (observed previously using that Swedish dataset and in this and earlier meta-analyses) persisted into the teenage/young adult years, and which specific tumors accounted for the overall risk reduction beyond childhood. Our meta-analysis of studies of aggregate childhood cancer risk in twins confirmed their approximate 15% reduction in cancer mortality and incidence. Further analysis of Swedish Multigeneration Register data for 1958 to 2002 suggested these reduced risks of cancer (particularly leukaemias and renal tumors) extended from childhood to young adult ages. Reduced risks of these and some other specific tumor types occurring across childhood/teenage/young adult years appeared to account for most of the overall risk reduction. Our results suggest a persistent reduction of overall childhood cancer risk in twins and that this extends into young adulthood. Risk reductions for several specific tumors might account for this and, although there are several potential explanations, intrauterine growth patterns of twins might be a major contributor.

A simplified configuration was developed to facilitate the mode transition process within an over-under Turbine-Based Combined Cycle (TBCC) inlet. Leveraging dynamic mesh technology, an unsteady numerical simulation of the mode transition was conducted, emphasising the flow characteristics of the mode transition and the impact of key similarity criteria numbers. The findings indicate that at an incoming Mach number of 2.0, the mode transition is paired with a continuous alteration in the capture mass flow of the high-speed duct. This continual change instigates the inlet unstarting, with subsequent flow characteristics being contingent on the historical effect, exhibiting a degree of hysteresis characteristics. When the scale effect is considered, it is observed that a larger model scale results in higher Reynolds (Re) and Strouhal (St) numbers. This directly contributes to a notable delay in the unstart moment, a decrease in the unstart interval, and an enlargement of the hysteresis loop. An examination of control variables reveals that the Re number marginally influences mode transition characteristics, while the St number’s effect constitutes approximately 90% of the scale effect. This conclusively demonstrates that the St number is the predominant similarity criterion number in the mode transition process.