Volunteer corn (Zea mays L.) is a competitive weed in corn-based cropping systems. Scientific literature does not exist about the water use of volunteer corn grown in different crops and irrigation systems. The objectives of this study were to characterize the growth and evapotranspiration (ETa) of volunteer corn in corn, soybean [Glycine max (L). Merr.], and sorghum [Sorghum bicolor (L.) Moench] under center-pivot irrigation (CPI) and subsurface drip irrigation (SDI) systems. Field experiments were conducted in south-central Nebraska in 2021 and 2022. Soil moisture sensors were installed at depths of 0 to 0.30, 0.30 to 0.60, and 0.60 to 0.90 m to track soil water balance and quantify seasonal total ETa. Corn was the most competitive, as volunteer corn had the lowest biomass, leaf area, and plant height compared with the fallow. Soybean was the least competitive with volunteer corn, as the plant height, biomass, and leaf area of volunteer corn in soybean were similar to fallow at 15, 30, 45, and 60 d after transplanting (DATr). Averaged across crop treatments, irrigation type did not affect volunteer corn growth at 15 to 45 DATr. Soil water depletion and ETa were similar across crop treatments with and without volunteer corn, as water was not a limiting factor in this study. The ETa of volunteer corn was the highest in soybean (623 mm), followed by sorghum (622 mm), and corn (617 mm) under CPI. The SDI had higher irrigation efficiency, because without affecting crop yield, it had 3%, 6%, and 8% lower ETa in soybean (605 mm), sorghum (585 mm), and corn (571 mm), respectively. Although soil water use did not differ with volunteer corn infestation, a soybean yield loss of 27% was observed, which suggests that volunteer corn may not compete for moisture under fully irrigated conditions; however, it can impact the crop yield potential due to competition for factors other than soil moisture.

Palmer amaranth (Amaranthus palmeri S. Watson) is a major biotic constraint in agronomic cropping systems in the United States. While crop–weed competition models offer a beneficial tool for understanding and predicting crop yield losses, within these models, certain weed biological characteristics and their responses to the environment are unknown. This limits understanding of weed growth in competition with crops under different irrigation methods and how competition for soil moisture affects crop growth parameters. This research measured the effect of center-pivot irrigation (CPI) and subsurface drip irrigation (SDI) on the actual evapotranspiration (ETa) of A. palmeri grown in maize (Zea mays L.), soybean [Glycine max (L.) Merr.], and fallow subplots. Twelve A. palmeri plants were alternately transplanted 1 m apart in the middle two rows of maize, soybean, and fallow subplots under CPI and SDI in 2019 and 2020 in south-central Nebraska. Maize, soybean, and fallow subplots without A. palmeri were included for comparison. Soil-moisture sensors were installed at 0-0.30, 0.30-0.60, and 0.60-0.90-m soil depths next to or between three A. palmeri and crop plants in each subplot. Soil-moisture data were recorded hourly from the time of A. palmeri transplanting to crop harvest. The results indicate differences in A. palmeri ETa between time of season (early, mid-, and late season) and crop type across 2019 and 2020. Although irrigation type did not affect subplot data, the presence of A. palmeri had an impact on subplot ETa across both years, which can be attributed to the variable relationship between volumetric soil water content (VWC) and ETa throughout the growing season due to advancing phenological stages and management practices. This study provides important and first-established baseline data and information about A. palmeri evapotranspiration and its relation to morphological features for future use in mechanistic crop–weed competition models.

Corn that is resistant to aryloxyphenoxypropionate, known commercially as Enlist™ corn, enables the use of quizalofop-p-ethyl (QPE) as a selective postemergence (POST) herbicide for control of glufosinate/glyphosate-resistant corn volunteers. Growers usually mix QPE with 2,4-D choline or glufosinate or both to achieve broad-spectrum weed control in Enlist corn. The objectives of this study were 1) to evaluate the efficacy of QPE applied alone or mixed with 2,4-D choline and/or glufosinate to control glufosinate/glyphosate-resistant corn volunteers in Enlist corn and 2) to determine the effect of application time (V3 or V6 growth stage of volunteer corn) of QPE-based treatments on volunteer corn control and Enlist corn injury and yield. Field experiments were conducted in Clay Center, NE, in 2021 and 2022. Quizalofop-p-ethyl (46 or 93 g ai ha−1) applied at the V3 or V6 growth stage controlled volunteer corn by ≥88% and ≥95% at 14 and 28 d after treatment (DAT), respectively. QPE (46 g ai ha−1) mixed with 2,4-D choline (800 g ae ha−1) produced 33% less than expected control of V3 volunteer corn in 2021, and 8% less than expected control of V6 volunteer corn in 2022 at 14 DAT. Volunteer corn control was improved by 7% to 9% using the higher rate of QPE (93 g ai ha−1) in a mixture with 2,4-D choline (1,060 g ae ha−1). QPE mixed with glufosinate had an additive effect and interactions in any combinations were additive beyond 28 DAT. Mixing 2,4-D choline can reduce QPE efficacy on glufosinate/glyphosate-resistant corn volunteers up to 14 DAT when applied at the V3 or V6 growth stage; however, the antagonistic interaction did not translate into corn yield loss. Increasing the rate of QPE (93 g ai ha−1) while mixing with 2,4-D choline can reduce antagonism.

Effective irrigation management is critical for future food supplies and the prosperity of producers engaged in irrigation production. Through a deficit irrigation field experiment, we determine the financial impact on producers caused by changing irrigation costs, corn prices, extreme weather events, and restricting irrigation levels. Results suggest that the optimal economic strategy within our constrained optimization model is to fully irrigate, with the economic impact highly dependent on commodity prices, restriction level, and irrigation costs. The greatest economic losses caused by irrigation restrictions come from decreases in yield. Some simulations resulted in negative profits, indicating that a switch to alternative crops requiring less irrigation may be warranted.

Weeds compete with crops for soil moisture, along with other resources, which can impact the germination, growth, and seed production of weeds; however, this impact has not been systematically recorded and synthesized across diverse studies. To address this knowledge gap, a global meta-analysis was conducted using 1,196 paired observations from 86 published articles assessing the effect of water stress on weed germination, growth characteristics, and seed production. These studies were conducted and published during 1970 through 2020 across four continents (Asia, Australia, Europe, and North America). Imposed water stress was expressed as solution osmotic potential (ψsolution), soil water potential (ψsoil), or soil moisture as percent field capacity. Meta-analysis revealed that water stress inhibits weed germination, growth, and seed production, and the quantitative response intensified with increasing water stress. A ψsolution greater than −0.8 MPa completely inhibits germination of both grass and broadleaf weeds. A ψsolution from −0.09 to −0.32 MPa reduces weed germination by 50% compared with the unstressed condition. Moderate soil water stress, equivalent to 30% to 60% field capacity, inhibits growth characteristics (branches or tillers per plant, leaf area, leaves per plant, plant height, root, and shoot biomass) by 33% and weed seed production by 50%. Severe soil water stress, below 30% field capacity, inhibits weed growth by 51% and seed production by 88%. Although water stress inhibits weed growth, it does not entirely suppress the ability to germinate, grow, and produce seeds, resulting in weed seedbank accumulation. This creates management challenges for producers, because weed seeds can survive in the soil for many years, depending on weed species and environmental conditions. Quantitative information compiled in this meta-analysis can be instrumental to model the weeds’ multidimensional responses to water stress and designing integrated weed management strategies for reducing the weed seedbank.

Velvetleaf (Abutilon theophrasti Medik.) is a troublesome broadleaf weed that competes with crops for resources such as soil moisture. Water stress can affect the ability of weed species to grow and produce seeds. The objective of this study was to determine the effect of degree of water stress on the growth and fecundity of A. threophrasti using soil moisture sensors under greenhouse conditions. Abutilon threophrasti seeds collected from a corn (Zea mays L.)–soybean [Glycine max (L.) Merr.] field were grown in silty clay loam soil, and plants were maintained at 100%, 75%, 50%, and 25% soil field capacity (FC) corresponding to no, light, moderate, and high water-stress conditions, respectively. Water was added daily to pots based on soil moisture levels detected by a Meter Group 5TM sensor to maintain the desired water-stress level required by treatment. Plants maintained at 100% FC had the maximum number of leaves (28 leaves plant−1), followed by 21 and 15 leaves plant−1 at 75% and 50% FC, respectively. Abutilon threophrasti at 100% and 75% FC achieved maximum plant height (108 to 123 cm) compared with 83 cm at 50% FC. Abutilon threophrasti maintained at 75% FC had the greatest growth index (79,907 cm3) followed by 72,197 cm3 at 100% FC and 64,256 cm3 at 50% FC. Seed production was similar at 100%, 75%, and 50% FC (288 to 453 seeds plant−1) compared with 2 seeds plant−1 at 25% FC. This is because the majority of plants maintained at 25% FC did not survive more than 77 d after transplanting. Seed germination was 96% to 100% at 100%, 75%, and 50% FC compared with 20% germination at 25% FC. Abutilon threophrasti can survive ≥50% FC continuous water-stress conditions, although with reduced leaf number, plant height, and growth index compared with 75% and 100% FC.

Palmer amaranth is the most problematic and troublesome weed in agronomic cropping systems in the United States. Acetolactate synthase (ALS) inhibitor and glyphosate-resistant (GR) Palmer amaranth has been confirmed in Nebraska and it is widespread in several counties. Soybean resistant to isoxaflutole/glufosinate/glyphosate has been developed that provides additional herbicide site of action for control of herbicide-resistant weeds. The objectives of this study were to evaluate herbicide programs for control of ALS inhibitor/GR Palmer amaranth and their effect on Palmer amaranth density and biomass, as well as soybean injury and yield in isoxaflutole/glufosinate/glyphosate–resistant soybean. Field experiments were conducted in a grower’s field infested with ALS inhibitor and GR Palmer amaranth near Carleton, Nebraska, in 2018 and 2019. Isoxaflutole applied alone or mixed with sulfentrazone/pyroxasulfone, flumioxazin/pyroxasulfone, or imazethapyr/saflufenacil/pyroxasulfone provided similar control (86%–99%) of Palmer amaranth 21 d after PRE (DAPRE). At 14 d after early-POST (DAEPOST), isoxaflutole applied PRE and PRE followed by (fb) POST controlled Palmer amaranth by 10% to 63% compared to 75% to 96% control with glufosinate applied EPOST in both years. A PRE herbicide fb glufosinate controlled Palmer amaranth 80% to 99% 21 d after late-POST (DALPOST) in 2018, and reduced density 89% to 100% in 2018 and 58% to 100% in 2019 at 14 DAEPOST. No soybean injury was observed from any of the herbicide programs tested in this study. Soybean yield in 2019 was relatively higher due to higher precipitation compared with 2018 with generally no differences between herbicide programs. This research indicates that herbicide programs are available for effective control of ALS inhibitor/GR Palmer amaranth in isoxaflutole/glufosinate/glyphosate-resistant soybean.

Velvetleaf is an economically important weed in popcorn production fields in Nebraska. Many PRE herbicides in popcorn have limited residual activity or provide partial velvetleaf control. There are a limited number of herbicides applied POST in popcorn compared with field corn, necessitating the evaluation of POST herbicides for control of velvetleaf. The objectives of this study were to (1) evaluate the efficacy and crop safety of labeled POST herbicides for controlling velvetleaf that survived S-metolachlor/atrazine applied PRE and (2) determine the effect of velvetleaf height on POST herbicide efficacy, popcorn injury, and yield. Field experiments were conducted in 2018 and 2019 near Clay Center, Nebraska. The experiments were arranged in a split-plot design with four replications. The main plot treatments were velvetleaf height (≤15 cm and ≤30 cm) and subplot treatments included a no-POST herbicide control, and 11 POST herbicide programs. Fluthiacet-methyl, fluthiacet-methyl/mesotrione, carfentrazone-ethyl, dicamba, and dicamba/diflufenzopyr provided greater than 96% velvetleaf control 28 d after treatment (DAT), reduced velvetleaf density to fewer than 7 plants m−2, achieved 99% to 100% biomass reduction, and had no effect on popcorn yield. Herbicide programs tested in this study provided greater than 98% control of velvetleaf 28 DAT in 2019. Most POST herbicide programs in this study provided greater than 90% control of up to 15 cm and up to 30 cm velvetleaf and no differences between velvetleaf heights in density, biomass reduction, or popcorn yield were observed, except with topramezone and nicosulfuron/mesotrione 28 DAT in 2018. On the basis of contrast analysis, herbicide programs with fluthiacet-methyl or dicamba provided better control than herbicide programs without them at 28 DAT in 2018. It is concluded that POST herbicides are available for control of velvetleaf up to 30-cm tall in popcorn production fields.

Understanding the critical time of weed removal (CTWR) is necessary for designing effective weed management programs in popcorn production that do not result in yield reduction. The objective of this study was to determine the CTWR in popcorn with and without a premix of atrazine and S-metolachlor applied PRE. Field experiments were conducted at the University of Nebraska–Lincoln, South Central Agricultural Laboratory near Clay Center, NE in 2017 and 2018. The experiment was laid out in a split-plot design with PRE herbicide as the main plot and weed removal timing as the subplot. Main plots included no herbicide or atrazine/S-metolachlor applied PRE. Subplot treatments included a weed-free control, a non-treated control, and weed removal timing at V3, V6, V9, V15, and R1 popcorn growth stages and then kept weed free throughout the season. A four-parameter log-logistic function was fitted to percentage popcorn yield loss and growing degree days separately to each main plot. The number of growing degree days, when 5% yield loss was achieved, was extracted from the model and compared between main plots. The CTWR was from the V4 to V5 popcorn growth stage in absence of PRE herbicide. With atrazine/S-metolachlor applied PRE, the CTWR was delayed until V10 to V15. It is concluded that, to avoid yield loss, weeds must be controlled before the V4 popcorn growth stage when no PRE herbicide is applied, and PRE herbicide, such as atrazine/S-metolachlor in this study, can delay the CTWR until the V10 growth stage.

Palmer amaranth (Amaranthus palmeri S. Watson) is the most problematic weed in agronomic crop production fields in the United States. The objective of this study was to determine the effect of degree of water stress on the growth and fecundity of A. palmeri using soil moisture sensors under greenhouse conditions. Two A. palmeri biotypes collected from Nebraska were grown in loam soil maintained at 100%, 75%, 50%, 25%, and 12.5% soil field capacity (FC) corresponding to no, light, moderate, high, and severe water stress levels, respectively. Water was regularly added to pots based on soil moisture levels detected by Watermark or Decagon 5TM sensors to maintain the desired water stress level. Amaranthus palmeri plants maintained at ≤25% FC did not survive more than 35 d after transplanting. Amaranthus palmeri at 100%, 75%, and 50% FC produced similar numbers of leaves (588 to 670 plant−1) based on model estimates; however, plants at 100% FC achieved a maximum height of 178 cm compared with 124 and 88 cm at 75% and 50% FC, respectively. The growth index (1.1×105 to 1.4×105 cm3 plant−1) and total leaf area (571 to 693 cm2 plant−1) were also similar at 100%, 75%, and 50% FC. Amaranthus palmeri produced similar root biomass (2.3 to 3 g plant−1) at 100%, 75%, and 50% FC compared with 0.6 to 0.7 g plant−1 at 25% and 12.5% FC, respectively. Seed production was greatest (42,000 seeds plant−1) at 100% FC compared with 75% and 50% FC (14,000 to 19,000 seeds plant−1); however, the cumulative seed germination was similar (38% to 46%) when mother plants were exposed to ≥50% FC. The results of this study show that A. palmeri can survive ≥50% FC continuous water stress conditions and can produce a significant number of seeds with no effect of on seed germination.

Palmer amaranth, a dioecious summer annual weed species, is the most troublesome weed in agronomic crop production systems in the United States. Palmer amaranth resistant to photosystem (PS) II- and 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors is of particular concern in south central Nebraska. The objectives of this study were to determine the effect of PRE followed by POST herbicide programs on PS II- and HPPD-inhibitor-resistant Palmer amaranth control, crop yield, and net economic return in conventional corn. A field study was conducted in 2014, 2015, and 2016 in a grower’s field infested with PS II- and HPPD-inhibitor-resistant Palmer amaranth near Shickley in Fillmore County, Nebraska. A contrast analysis suggested that mesotrione+S-metolachlor+atrazine applied PRE provided 83% Palmer amaranth control at 21 d after application compared to 78 and 72% control with pyroxasulfone+fluthiacet-ethyl+atrazine and saflufenacil+dimethenamid-P, respectively. Most of the PRE followed by POST herbicide programs provided ≥85% Palmer amaranth control. Based on contrast analysis, POST application of dicamba+diflufenzopyr provided 93% Palmer amaranth control compared to 87, 79, and 42% control with dicamba, dicamba+halosulfuron, and acetochlor, respectively, at 28 d after POST. All PRE followed by POST herbicide programs, aside from mesotrione+S-metolachlor+atrazine followed by acetochlor (2,530 to 7,809 kg ha−1), provided 9,550 to 10,500 kg ha−1 corn yield compared with 2,713 to 6,110 kg ha−1 from nontreated control. Similarly, PRE followed by POST herbicide programs, except for mesotrione+S-metolachlor+atrazine followed by acetochlor ($191 and $897 ha−1), provided similar net return of $427 to $707 ha−1 and $1,127 to $1,727 ha−1 in 2014 and 2015-16, respectively. It is concluded that herbicide programs based on multiple sites of action are available for control of PS II- and HPPD-inhibitor-resistant Palmer amaranth in conventional corn.

Field and greenhouse experiments were conducted in Nebraska to (1) confirm the 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting resistant-waterhemp biotype (HPPD-RW) by quantifying the resistance levels in dose-response studies, and (2) to evaluate efficacy of PRE-only, POST-only, and PRE followed by POST herbicide programs for control of HPPD-RW in corn. Greenhouse dose-response studies confirmed that the suspected waterhemp biotype in Nebraska has evolved resistance to HPPD-inhibiting herbicides with a 2- to 18-fold resistance depending upon the type of HPPD-inhibiting herbicide being sprayed. Under field conditions, at 56 d after treatment, ≥90% control of the HPPD-RW was achieved with PRE-applied mesotrione/atrazine/S-metolachlor+acetochlor, pyroxasulfone (180 and 270 g ai ha−1), pyroxasulfone/fluthiacet-methyl/atrazine, and pyroxasulfone+saflufenacil+atrazine. Among POST-only herbicide programs, glyphosate, a premix of mesotrione/atrazine tank-mixed with diflufenzopyr/dicamba, or metribuzin, or glufosinate provided ≥92% HPPD-RW control. Herbicide combinations of different effective sites of action in mixtures provided ≥86% HPPD-RW control in PRE followed by POST herbicide programs. It is concluded that the suspected waterhemp biotype is resistant to HPPD-inhibiting herbicides and alternative herbicide programs are available for effective control in corn. The occurrence of HPPD-RW in Nebraska is significant because it limits the effectiveness of HPPD-inhibiting herbicides.

The nonnative biotype of common reed has invaded wetlands in many states including Nebraska, especially along the Platte River from Wyoming to the eastern edge of Nebraska. Therefore, three studies (disking followed by herbicide, mowing followed by herbicide, and herbicide followed by mechanical treatment) were conducted for 3 yr (2008 to 2010) at three locations in Nebraska. The objective was to evaluate common reed control along the Platte River using an integrated management approach based on herbicides (glyphosate or imazapyr), mowing, and disking, either applied alone or in combination. The level of weed control was determined by visual rating, percent flowering, and stem density. On the basis of visual rating, disking and mowing used alone provided common reed control for only a few months. However, the control was significantly prolonged (e.g., at least three seasons) when disking and mowing were combined with herbicide applications. Disking followed by herbicide and mowing followed by herbicide significantly reduced flowering and plant densities (P = 0.0001) compared to the untreated check. These results suggest that a combination of weed control methods has potential to control common reed.

Common waterhemp is one of the most commonly encountered and troublesome weeds in the midwestern United States. It is well known that water stress adversely affects crop growth and yield; however, the effects of water stress on weed growth and seed production are poorly understood. The objective of this study was to determine the effects of degree and duration of water stress on growth, development, and fecundity of two common waterhemp biotypes in greenhouse experiments conducted at the University of Nebraska–Lincoln. No difference was observed in growth, development, and seed production between two biotypes in response to degree and duration of water stress; therefore, data were combined. The degree of water stress study included five treatments, where the amount of water applied to each pot at 2-d interval was equivalent to 100, 75, 50, 25, and 12.5% of pot (soil) water content. The highest plant height (163 cm), number of leaves (231 plant−1), and growth index (4.4 × 105 cm3) were recorded at 100% of pot water content (no water stress). Similarly, aboveground biomass, total leaf area, and seed production reached their maximum at 100% of pot water content treatment, whereas they were reduced as degree of water stress increased. The study of water stress duration included five treatments, where amount of water applied to each pot at 2-, 4-, 6-, 8-, and 10-d intervals was equivalent to 100% of pot water content. The highest plant height (150 cm), number of leaves (210 plant−1), and growth index (3.8 × 105 cm3) were observed at 2-d interval of water stress, whereas seed production was similar at 2-d (36,549 seeds plant−1) and 4-d (34,176 seeds plant−1) intervals. This study shows that common waterhemp has capacity to survive and reproduce even under a higher degree and duration of water stress.

Glyphosate-resistant common waterhemp is a difficult-to-control annual broadleaf weed that has become a serious management challenge for growers in Nebraska and other states in the United States. The objectives of this study were to confirm glyphosate-resistant common waterhemp in Nebraska by quantifying level of resistance in a dose-response study, and to determine the sensitivity and efficacy of POST soybean herbicides for controlling suspected glyphosate-resistant common waterhemp biotypes. Seeds of suspected glyphosate-resistant common waterhemp biotypes were collected from seven eastern Nebraska counties. Greenhouse dose-response experiments were conducted to evaluate the response of common waterhemp biotypes to nine rates of glyphosate (0 to 16×). Common waterhemp biotypes were 3- to 39-fold resistant to glyphosate depending on the biotype being investigated and the susceptible biotype used for comparison. Results of the POST soybean herbicides efficacy experiment suggested that glyphosate-resistant biotypes, except a biotype from Pawnee County, had reduced sensitivity to acetolactate synthase (ALS)–inhibiting herbicides (chlorimuron-ethyl, imazamox, imazaquin, imazethapyr, and thifensulfuron-methyl). Glufosinate and protoporphyrinogen oxidase (PPO)–inhibiting herbicides (acifluorfen, fluthiacet-methyl, fomesafen, and lactofen) provided ≥ 80% control of glyphosate-resistant common waterhemp at 21 d after treatment (DAT). This study confirmed the first occurrence of glyphosate-resistant common waterhemp in Nebraska, and also revealed reduced sensitivity to ALS-inhibiting herbicides in most of the biotypes tested in this study.

The evolution of glyphosate and acetolactate synthase (ALS) inhibitor-resistant common waterhemp in the Midwestern United States has reduced the number of effective POST herbicide options for management of this problem weed in glyphosate-resistant soybean. Moreover, common waterhemp emerges throughout the crop growing season, justifying the need to evaluate herbicide programs that provide season-long control. The objectives of this study were to compare POST-only and PRE followed by (fb) POST herbicide programs for control of glyphosate-resistant common waterhemp in glyphosate-resistant soybean. Field experiments were conducted in 2013 and 2014 in Dodge County, NE, in a field infested with glyphosate-resistant common waterhemp. Programs containing PRE herbicides resulted in ≥83% control of common waterhemp and densities of ≤35 plantsm–2 at 21 d after PRE (DAPRE). Post-only herbicide programs resulted in <70% control and densities of 107 to 215 plants m–2 at 14 d after early-POST (DAEPOST) treatment. PRE fb POST herbicide programs, including saflufenacil plus imazethapyr plus dimethenamid-P, sulfentrazone plus cloransulam, or S-metolachlor plus metribuzin, fb fomesafen plus glyphosate; S-metolachlor plus fomesafen fb acifluorfen plus glyphosate resulted in >90% control of glyphosate-resistant common waterhemp throughout the growing season, reduced density to ≤7plantsm–2, ≥92% biomass reduction, and soybean yield >2,200kg ha–1. Averaged across herbicide programs, common waterhemp control was 84%, and density was 15 plants m–2 with PRE fb POST herbicide programs compared with 42% control, and density of 101 plants m–2 with POST-only herbicide programs at harvest. Results of this study indicated that PRE fb POST herbicide programs with effective modes of action exist for season-long control of glyphosate-resistant common waterhemp in glyphosate-resistant soybean.

The deep-sea portunid crab Bathynectes maravigna, previously known from the Aegean Sea coast of Turkey, is reported for the first time from Mersin Bay on the Levantine Sea coast of Turkey. The record is based on three adult males and two adult females collected by trawling on a sandy bottom at depths between 300 and 450 m in Mersin Bay.