Contents

pp vii-xii

Preface to the second edition

pp xiii-xiv

1 - Introduction

pp 1-8

Part I - The basis of genetic engineering

pp 9-10

2 - Introducing molecular biology

pp 11-26

3 - Working with nucleic acids

pp 27-42

4 - The tools of the trade

pp 43-54

Part II - The methodology of gene manipulation

pp 55-56

5 - Host cells and vectors

pp 57-86

6 - Cloning strategies

pp 87-114

7 - The polymerase chain reaction

pp 115-131

8 - Selection, screening and analysis of recombinants

pp 132-150

Part III - Genetic engineering in action

pp 151-152

9 - Understanding genes and genomes

pp 153-177

10 - Genetic engineering and biotechnology

pp 178-196

11 - Medical and forensic applications of gene manipulation

pp 197-223

12 - Transgenic plants and animals

pp 224-246

13 - The other sort of cloning

pp 247-258

14 - Brave new world or genetic nightmare?

pp 259-262

Suggestions for further reading

pp 263-265

Using the World Wide Web

pp 266-269

Glossary

pp 270-286

Index

pp 287-292