Key Experiments in Practical Developmental Biology

doi:10.1017/CBO9780511546204

Originally published in 2005, this unique resource presents 27 easy-to-follow laboratory exercises for use in student practical classes in developmental biology. These experiments provide key insights into developmental questions, and many of them are described by the leaders in the field who carried out the original research. This book intends to bridge the gap between experimental work and the laboratory classes taken at the undergraduate and post-graduate levels. All chapters follow the same format, taking the students from materials and methods, through results and discussion, so that they learn the underlying rationale and analysis employed in the research. The book will be an invaluable resource for graduate students and instructors teaching practical developmental biology courses. Chapters include teaching concepts, discussion of the degree of difficulty of each experiment, potential sources of failure, as well as the time required for each experiment to be carried out in a class with students.

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Contents

Frontmatter
pp i-vi
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Contents
pp vii-x
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List of contributors
pp xi-xiv
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Preface
pp xv-xvi
- By Manuel Marí-Beffa, University of Málaga
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Introduction
pp 1-3
- By Manuel Marí-Beffa, University of Málaga, Jennifer Knight, University of Colorado, Boulder
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SECTION I - GRAFTINGS
- By Manuel Marí-Beffa, University of Málaga, Jennifer Knight, University of Colorado, Boulder
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1 - Two developmental gradients control head formation in hydra
pp 4-22
- By H. R. Bode, Developmental Biology Center and Department of Developmental and Cell Biology, University of California Irvine, 5205 McGaugh Hill, Irvine, California 92697, USA
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2 - Embryonic regulation and induction in sea urchin development
pp 23-36
- By C. A. Ettensohn, Department of Biological Sciences, Science and Technology Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
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3 - The isthmic organizer and brain regionalization in chick embryos
pp 37-49
- By D. Echevarría, Instituto de Neurociencias de Alicante (UMH-CSIC), University of Miguel Hernández, Campus de San Juan, Carretera de Valencia, Km. 87, E-03550 Alicante, Spain, S. Martìnez, Instituto de Neurociencias de Alicante (UMH-CSIC), University of Miguel Hernández, Campus de San Juan, Carretera de Valencia, Km. 87, E-03550 Alicante, Spain
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SECTION II - SPECIFIC CHEMICAL REAGENTS
- By Manuel Marí-Beffa, University of Málaga, Jennifer Knight, University of Colorado, Boulder
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4 - Chemotaxis of aggregating Dictyostelium cells
pp 50-66
- By G. Gerisch, Max-Planck-Institut für Biochimie, Am Klopferspitz 18a, D-82152 Martinsried, Germany, M. Ecke, Max-Planck-Institut für Biochimie, Am Klopferspitz 18a, D-82152 Martinsried, Germany
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5 - Inhibition of signal transduction pathways prevents head regeneration in hydra
pp 67-76
- By L. M. Salgado, CINVESTAV-IPN, Dpto. Biochemistry, Apartado Postal 14-740, 07000 México, D.F., México
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6 - Retinoic acid during limb regeneration
pp 77-84
- By M. Maden, MRC Centre for Developmental Neurobiology, 4th floor New Hunt's House, King's College London, Guy's Campus, London Bridge, London SE1 1UL, UK
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SECTION III - BEAD IMPLANTATION
- By Manuel Marí-Beffa, University of Málaga, Jennifer Knight, University of Colorado, Boulder
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7 - Experimental manipulations during limb development in avian embryos
pp 85-98
- By Y. Gañán, Área Anatomía y Embriología Humanas, Departamento de Ciencias Morfológicas y Biología Celular y Animal, Facultad de Medicina, Universidad de Extremadura, E-06071 Badajoz, Spain, J. Rodríguez-León, Instituto Gulbenkian de Ciência, Rua da Quinta Grande n° 6, Apt. 14, 2780-901 Oeiras, Portugal, D. Macías, Área Anatomía y Embriología Humanas, Departamento de Ciencias Morfológicas y Biología Celular y Animal, Facultad de Medicina, Universidad de Extremadura, E-06071 Badajoz, Spain
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8 - Induction of ectopic limb outgrowth in chick with FGF-8
pp 99-105
- By Á. Raya, Gene Expression Laboratories, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037-1099, USA, C. Rodríguez Esteban, Gene Expression Laboratories, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037-1099, USA, J. C. Izpisúa Belmonte, Gene Expression Laboratories, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037-1099, USA
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SECTION IV - NUCLEIC ACID INJECTIONS
- By Manuel Marí-Beffa, University of Málaga, Jennifer Knight, University of Colorado, Boulder
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9 - RNAi techniques applied to freshwater planarians (Platyhelminthes) during regeneration
pp 106-116
- By D. Bueno, Departament de Genètica, Facultat Biologia, Universitat Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain, R. Romero, Departament de Genètica, Facultat Biologia, Universitat Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain, E. Saló, Departament de Genètica, Facultat Biologia, Universitat Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain
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10 - Microinjection of Xenopus embryos
pp 117-126
- By R. J. Garriock, Department of Cell Biology and Anatomy, LSN 444, University of Arizona College of Medicine, P.O. Box 245044, 1501 N. Campbell Avenue, Tucson, Arizona, 85743, USA, P. A. Krieg, Department of Cell Biology and Anatomy, LSN 444, University of Arizona College of Medicine, P.O. Box 245044, 1501 N. Campbell Avenue, Tucson, Arizona 85743, USA
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SECTION V - GENETIC ANALYSIS
- By Manuel Marí-Beffa, University of Málaga, Jennifer Knight, University of Colorado, Boulder
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11 - Segmental specification in Drosophila melanogaster
pp 127-142
- By L. De Navas, Centro de Biología Molecular “Severo Ochoa”, Universidad Autónoma de Madrid, Cantoblanco, E-28049 Madrid, Spain, M. Suzanne, Centro de Biología Molecular “Severo Ochoa”, Universidad Autónoma de Madrid, Cantoblanco, E-28049 Madrid, Spain, D. Foronda, Centro de Biología Molecular “Severo Ochoa”, Universidad Autónoma de Madrid, Cantoblanco, E-28049 Madrid, Spain, E. Sánchez-Herrero, Centro de Biología Molecular “Severo Ochoa”, Universidad Autónoma de Madrid, Cantoblanco, E-28049 Madrid, Spain
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12 - Genetic analysis of flower development in Arabidopsis thaliana. The ABC model of floral organ identity determination
pp 143-152
- By J. L. Riechmann, Gene Expression Center, Biology, California Institute of Technology, 102B Kerckhoff M/C 156-29, Pasadena, California 91125, USA
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13 - Genetic analysis of vulva development in C. elegans
pp 153-166
- By S. Canevascini, Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland
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SECTION VI - CLONAL ANALYSIS
- By Manuel Marí-Beffa, University of Málaga, Jennifer Knight, University of Colorado, Boulder
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14 - The role of the gene apterous in the development of the Drosophila wing
pp 167-177
- By F. J. Díaz-Benjumea, Centro de Biología Molecular “Severo Ochoa”, Universidad Autónoma de Madrid, Cantoblanco, E-28049 Madrid, Spain
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15 - Extramacrochaetae, an example of a gene required for control of limb size and cell differentiation during wing morphogenesis in Drosophila
pp 178-189
- By A. Baonza, MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
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16 - Hedgehog transduction pathway is involved in pattern formation in Drosophila melanogaster tergites
pp 190-204
- By M. Marí-Beffa, Department of Cell Biology, Genetics and Physiology, Faculty of Science, University of Málaga, 29071 Málaga, Spain
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SECTION VII - IN SITU HYBRIDIZATION
- By Manuel Marí-Beffa, University of Málaga, Jennifer Knight, University of Colorado, Boulder
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17 - Retinoic acid signalling controls anteroposterior patterning of the zebrafish hindbrain
pp 205-216
- By G. Begemann, Department of Biology, University of Konstanz, Universitaetsstr. 10, D-78464 Konstanz, Germany
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18 - Left–right asymmetry in the mouse
pp 217-230
- By M. Blum, University of Hohenheim, Institute of Zoology (220), Garbenstrasse 30, D-70593 Stuttgart, Germany, A. Schweickert, University of Hohenheim, Institute of Zoology (220), Garbenstrasse 30, D-70593 Stuttgart, Germany, C. Karcher, University of Hohenheim, Institute of Zoology (220), Garbenstrasse 30, D-70593 Stuttgart, Germany
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