Sperm Collection and Processing Methods: A Practical Guide

doi:10.1017/CBO9780511545184

This practical guide to sperm collection and processing methods demonstrates how successful these procedures are to overcome even severe infertility. This compilation, prepared by an interdisciplinary team of experts, provides clinical and laboratory professionals in reproductive medicine with a comprehensive overview and a useful source of reference. Difficulties with sperm procurement can involve biological deficiencies with sperm creation, delivery, or both. The various procedures described in this text are all designed for one purpose: acquisition of as many viable sperm as possible, given initial patient conditions. Once sperm are collected, a myriad of processing methods are then available to optimize their fertilization potential. Recent advances have made fertility conditions once deemed hopeless a thing of the past. Most sterility cases are now either treatable or entirely circumvented through various clinical and laboratory techniques described in this volume, including surgical correction, hormone treatment, and various sperm enhancement technologies.

"[A] clear...well written and helpful guide to the practical management of a range of clinical and laboratory situations, some of which are relatively infrequently encountered." Stewart Irvine, Human Fertility

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Contents

Frontmatter
pp i-iv
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Contents
pp v-vi
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List of contributors
pp vii-ix
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Acknowledgment
pp x-x
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Introduction
pp 1-8
- By Rajasingam S. Jeyendran, Northwestern University Medical School, Chicago, Illinois, USA
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Part 1 - Sperm procurement methods
pp 9-14
- By Rajasingam S. Jeyendran, Northwestern University Medical School, Chicago, Illinois, USA, Craig Niederberger, University of Illinois at Chicago, Chicago, Illinois, USA
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Patient semen collection
pp 15-23
- By Rajasingam S. Jeyendran, Northwestern University Medical School, Chicago, Illinois, USA, Jane M. Nani, Advanced Reproductive Health Centers, Ltd, North Chicago, Illinois USA
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Patient semen collection with assistance
pp 24-37
- By Jane M. Nani, Advanced Reproductive Health Centers, Ltd, North Chicago, Illinois, USA, Rajasingam S. Jeyendran, Northwestern University Medical School, Chicago, Illinois, USA
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Clinical semen collection
pp 38-84
- By David Chen, Northwestern University Medical School, Chicago, Illinois, USA, Robert E. Brannigan, Northwestern University Medical School, Chicago, Illinois, USA, Rajasingam S. Jeyendran, Northwestern University Medical School, Chicago, Illinois, USA
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Clinical sperm collection
pp 85-104
- By Richard A. Schoor, University of Illinois at Chicago, Chicago, Illinois, USA, Lawrence S. Ross, University of Illinois at Chicago, Chicago, Illinois, USA, Craig Niederberger, University of Illinois at Chicago, Chicago, Illinois, USA
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Part 2 - Sperm processing methods
pp 105-110
- By Rajasingam S. Jeyendran, Northwestern University Medical School, Chicago, Illinois, USA, X. John Zhang, Northwestern University Medical School, Chicago, Illinois, USA
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General procedures: sperm washing, treatment, and cryopreservation
pp 111-122
- By Rajasingam S. Jeyendran, Northwestern University Medical School, Chicago, Illinois, USA, Andrew A. Ruiz, Hinsdale Center for Reproduction, Hinsdale, Illinois, USA, X. John Zhang, Northwestern University Medical School, Chicago, Illinois, USA
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Sperm processing procedures for intrauterine insemination
pp 123-140
- By Andrew A. Ruiz, Hinsdale Center for Reproduction, Hinsdale, Illinois, USA, Rajasingam S. Jeyendran, Northwestern University Medical School, Chicago, Illinois, USA
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Sperm processing procedures for assisted reproductive technology
pp 141-153
- By X. John Zhang, Northwestern University Medical School, Chicago, Illinois, USA, Rajasingam S. Jeyendran, Northwestern University Medical School, Chicago, Illinois, USA
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Conclusion
pp 154-155
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Further Reading
pp 156-156
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Index
pp 157-160
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Sperm Collection and Processing Methods

A Practical Guide

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Contents

Frontmatter
pp i-iv

Contents
pp v-vi

List of contributors
pp vii-ix

Acknowledgment
pp x-x

Introduction
pp 1-8

Part 1 - Sperm procurement methods
pp 9-14

Patient semen collection
pp 15-23

Patient semen collection with assistance
pp 24-37

Clinical semen collection
pp 38-84

Clinical sperm collection
pp 85-104

Part 2 - Sperm processing methods
pp 105-110

General procedures: sperm washing, treatment, and cryopreservation
pp 111-122

Sperm processing procedures for intrauterine insemination
pp 123-140

Sperm processing procedures for assisted reproductive technology
pp 141-153

Conclusion
pp 154-155

Further Reading
pp 156-156

Index
pp 157-160

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Sperm Collection and Processing Methods

A Practical Guide

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