The PCR Revolution: Basic Technologies and Applications

doi:10.1017/CBO9780511818974

The invention of the polymerase chain reaction (PCR) won the Nobel Prize in Chemistry in 1994 and remains one of the most important scientific discoveries of the twentieth century. More than 50,000 researchers in the United States use PCR replication technology, yet there is a significant absence of any recent publications on this subject. In this book, Dr Stephen A. Bustin, a world-renowned PCR expert, examines in detail the latest innovations and the overall impact of PCR on many areas of molecular research. The book contains personal reflections, opinions, and comments by leading authorities on the many applications of the PCR and how this technology has revolutionised their respective areas of interest. Bustin explains the ways in which PCR has overcome many obstacles in life science and clinical research and charts the PCR's development from time-consuming, low throughput, non-quantitative procedure to today's rapid, high throughput, quantitative super method.

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Contents

Frontmatter
pp i-iv
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Contents
pp v-vi
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Contributors
pp vii-x
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Foreword by Russell Higuchi
pp xi-xii
- By Russell Higuchi
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Preface
pp xiii-xviii
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I - BASIC TECHNOLOGIES
pp 1-2
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1 - Real-time polymerase chain reaction
pp 3-11
- By Mickey Williams
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2 - Thermostable enzymes used in polymerase chain reaction
pp 12-18
- By Sudip K. Rakshit
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3 - Inventing molecular beacons
pp 19-47
- By Fred Russell Kramer, Salvatore A. E. Marras, Sanjay Tyagi
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4 - Rapid polymerase chain reaction and melting analysis
pp 48-69
- By Carl T. Wittwer, Randy P. Rasmussen, Kirk M. Ririe
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5 - Polymerase chain reaction and fluorescence chemistries: deoxyribonucleic acid incarnate
pp 70-79
- By Ben Sowers
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6 - Analysis of microribonucleic acid expression by quantitative real-time polymerase chain reaction
pp 80-87
- By Vladimir Benes, Jens Stolte, David Ibberson, Mirco Castoldi, Martina Muckenthaler
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7 - Miniaturized polymerase chain reaction for quantitative clinical diagnostics
pp 88-109
- By Melissa Mariani, Lin Chen, Philip J. Day
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8 - The road from qualitative to quantitative assay: What is next?
pp 110-128
- By Michael W. Pfaffl
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9 - Taking control of the polymerase chain reaction
pp 129-152
- By Tania Nolan, Tanya Novak, Jim Huggett
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II - APPLICATIONS
pp 153-154
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10 - Polymerase chain reaction–based methods for the detection of solid tumor cancer cells for clinical diagnostic and prognostic assays
pp 155-172
- By Susan A. Burchill
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11 - Polymerase chain reaction and infectious diseases
pp 173-188
- By Jim Huggett
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12 - Polymerase chain reaction and respiratory viruses
pp 189-211
- By Ian M. Mackay
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13 - Polymerase chain reaction and severe acute respiratory syndrome
pp 212-228
- By Weijun Chen, Yang Huanming
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14 - The MMR vaccine, measles virus, and autism – A cautionary tale
pp 229-242
- By Stephen A. Bustin
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15 - Noninvasive prenatal diagnosis using cell-free fetal nucleic acids in maternal plasma
pp 243-253
- By Y. M. Dennis Lo
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16 - Polymerase chain reaction–based analyses of nucleic acids from archival material
pp 254-261
- By Ulrich Lehmann
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17 - Microarrays and quantitative real-time reverse transcriptase–polymerase chain reaction
pp 262-275
- By Elisa Wurmbach
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18 - Polymerase chain reaction in the detection of genetic variation
pp 276-283
- By Pui-Yan Kwok
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19 - Polymerase chain reaction: A blessing and a curse for ancient deoxyribonucleic acid research
pp 284-300
- By Michael Hofreiter, Holger Römpler
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Index
pp 301-307
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Plate section
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The PCR Revolution

Basic Technologies and Applications

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