The Greater Chassahowitzka black bear population is the smallest documented in North America with fewerthan 20 individuals. Its future depends on landscape linkages with other bear populations that are separated bydenatured habitat. We used a Geographic Information System (GIS) to identify potential landscape linkagesbetween this isolated population and six others in Florida. Pathway lengths ranged from 60–194 km withvarying potentials for facilitating black bear dispersal. Each pathway incorporated 35Ð88% conservation landand encountered at least 11 dispersal bottlenecks. Even pathways that incorporated extensive conservationland encountered bottlenecks that make these linkages potentially unviable. All six pathways, however, passedthrough ≥95% core black bear habitat. Thus, the infrastructure for a conservation network is still largelyintact. The Suwannee pathway provides the best opportunity to restore connectivity between the GreaterChassahowitzka Ecosystem (GCE) and a southward colonising bear population in the Big Bend region.However, intensification of development poses an immediate threat to maintaining connectivity between theGCE and other bear populations in Florida. Through immediate strategic planning and active conservationand restoration measures, many of the generated pathways can provide long-term connectivity. Least-cost pathanalyses can aid in the conservation of wide-ranging animals by providing managers with a science-based,empirically derived blueprint of potential landscape linkages.