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The development of oral fluid-based diagnostics and applications in veterinary medicine

  • John R. Prickett (a1) and Jeffrey J. Zimmerman (a1)
Abstract
Abstract

The purpose of this review was to discuss the history of the development and implementation of oral fluid diagnostics for infectious diseases of humans and domestic animals. The use of oral fluid for the assessment of health and diagnosis of disease in humans and animals has a surprisingly long history. As early as 1909, Pollaci and Ceraulo reported sensitive and specific agglutination of ‘Micrococcus melitensis’ (Brucella melitensis) by oral fluid from patients diagnosed with Malta Fever. A 1986 report of the detection of antibodies against human immunodeficiency virus (HIV) in oral fluid from patients with acquired immunodeficiency syndrome (AIDS) marked the start of a remarkably rapid series of developments in oral fluid-based assays. Cumulatively, the literature strongly supports implementation of oral fluid-based diagnostics in veterinary diagnostic medicine. Pathogen-specific IgA, IgM and IgG antibodies have all been demonstrated in oral fluid collected from diverse domestic animal species in response to infection. A variety of infectious agents, both local and systemic, are shed in oral fluid, including some of the most economically significant pathogens of production animals (e.g. foot-and-mouth disease virus, classical swine fever virus and porcine reproductive and respiratory syndrome virus) Ultimately, point-of-care rapid assays (i.e. cow-side, sow-side or pen-side tests) and access to real-time infectious disease data will revolutionize our delivery of health management services.

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*Corresponding author. E-mail: prickett@iastate.edu
References
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Amado LA, Villar LM, de Paula VS, de Almeida AJ and Gaspar AMC (2006). Detection of hepatitis A, B, and C virus-specific antibodies using oral fluid for epidemiological studies. Memórias do Instituto Oswaldo Cruz 101: 149155.
Archetti I, Amadori M, Donn A, Salt J and Lodetti E (1995). Detection of foot-and-mouth disease virus-infected cattle by assessment of antibody response in oropharyngeal fluids. Journal of Clinical Microbiology 33: 7984.
Archibald DW, Zon L, Groopman JE, McLane MF and Essex M (1986). Antibodies to human T-lymphotropic virus type III (HTLV-III) in saliva of acquired immunodeficiency syndrome (AIDS) patients and in persons at risk for AIDS. Blood 67: 831834.
Atkinson J, Dawes C, Ericson T, Fox PC, Gandara BK, Malamud D, Mandel ID, Navazesch M and Tabak LA (1993). Guidelines for saliva nomenclature and collection. Annals of the New York Academy of Sciences 694: xixii.
Baguley SDK, Horner PJ, Maple PAC and Stephenson L (2005). An oral fluid test for syphilis. International Journal of STD and AIDS 16: 299301.
Beckenkamp G (1985). Distribution pattern of the cellular oral immune system in the major and minor salivary glands, immunochemical findings. HNO 33: 196203.
Ben Salah A, Zaâtour A, Pomery L, Cohen BJ, Brown DW and Andrews N (2003). Validation of a modified commercial assay for the detection of rubella-specific IgG in oral fluid for use in population studies. Journal of Virological Methods 114: 151158.
Berger R, Ainbender E, Hodes HL, Zepp HD and Hevizy MM (1967). Demonstration of IgA polioantibody in saliva, duodenal fluid and urine. Nature 214: 420422.
Brandtzaeg P (1975). Human secretory immunoglobulin M an immunochemical and immunohistochemical study. Immunology 29: 559570.
Brandtzaeg P (1981). Transport models for secretory IgA and secretory IgM. Clinical and Experimental Immunology 44: 221232.
Brandtzaeg P (1989). Salivary immunoglobulins. In: Tenovuo JO (ed.) Human Saliva: Clinical Chemistry and Microbiology. Boca Raton, FL: CRC Press, pp. 151.
Branson B (2007). State of the art for diagnosis of HIV infection. Clinical Infectious Diseases 45 (suppl. 4): S221S225.
Brill N and Björn H (1959). Passage of tissue fluid into human gingival pockets. Acta Odontologica Scandinavica 17: 1121.
Brill N and Krasse BO (1958). The passage of tissue fluid into the clinically healthy gingival pocket. Acta Odontologica Scandinavica 16: 233245.
Brown DWG, Ramsay ME, Richards AF and Miller E (1994). Salivary diagnosis of measles: a study of notified cases in the United Kingdom, 1991–3. British Medical Journal 308: 10151017.
Cameron SO and Carman WF (2005). The use of Orasure® collection device for hepatitis virus testing in health care settings. Journal of Clinical Virology 34 (suppl. 1): S22S28.
Challacombe S, Percival R and Marsh P (1995). Age-related changes in Immunoglobulin isotypes in whole and parotid saliva and serum in healthy individuals. Oral Microbiology and Immunology 10: 202207.
Challacombe S, Rahman D and O'Hagan D (1997). Salivary, gut, vaginal and nasal antibody responses after oral immunization with biodegradable microparticles. Vaccine 15: 169175.
Challacombe S, Russel M, Hawkes J, Bergmeier L and Lehner T (1978). Passage of immunoglobulins from plasma to the oral cavity in rhesus monkeys. Immunology 35: 923931.
Chang CK, Cohen ME and Bienek DR (2009). Efficiency of oral fluid collection devices in extracting antibodies. Oral Microbiology and Immunology 24: 231235.
Chiappin S, Antonelli G, Gatti R and De Palo EF (2007). Saliva specimen: A new laboratory tool for diagnostic and basic investigation. Clinica Chimica Acta 383: 3040.
Chiba Y and Nakao T (1972). Mumps virus neutralizing antibody in saliva following natural infection. The Tohoku Journal of Experimental Medicine 106: 7581.
Coleman R and Appleman M (1953). Antibodies of syphilis in saliva and serum. Journal of Dental Research 32: 294297.
Collot S, Petit B, Bordessoule D, Alain S, Touati M, Denis F and Ranger-Rogez S (2002). Real-time PCR for quantification of human herpesvirus 6 DNA from lymph nodes and saliva. Journal of Clinical Microbiology 40: 24452451.
Connolly C, Shisana O, Colvin M and Stoker D (2004). Epidemiology of HIV in South Africa – results of a national, community-based survey. South African Medical Journal 94: 776781.
Côrtes V, de Oliveira M and Peixoto Z (1979). Use of saliva in the diagnosis of natural canine rabies. Arquivos do Instituto Biológico 46: 131133.
Corthier G (1976). Swine fever: influence of passive immunity on pig immune response following vaccination with a live virus vaccine (Thiverval strain). Annals of Veterinary Research 7: 361372.
Corthier G and Aynaud J (1977). Comparison of the immune response in serum and bucco-pharyngeal secretions following immunization by different routes with a live hog cholera virus vaccine (Thiverval strain). Annals of Veterinary Research 8: 159165.
Crawford J, Taubman M and Smith D (1975). Minor salivary glands as a major source of secretory immunoglobulin A in the human oral cavity. Science 190: 12061209.
Cuzzubbo AJ, Vaughn DW, Nisalak A, Suntayakorn S, Aaskov J and Devine PL (1998). Detection of specific antibodies in saliva during Dengue infection. Journal of Clinical Microbiology 36: 37373739.
de Azevedo Neto R, Richards A, Nokes D, Silveira A, Cohen B, Passos S, de Souza V, Brown D, Pannuti C and Massad E (1995). Salivary antibody detection in epidemiological surveys: a pilot study after a mass vaccination campaign against rubella in São Paulo, Brazil. Transactions of the Royal Society of Tropical Medicine and Hygiene 89: 115118.
da Silva MV, Dias Camargo E, Vaz AJ and Batista L (1992). Immunodiagnosis of human leptospirosis using saliva. Transactions of the Royal Society of Tropical Medicine and Hygiene 86: 560561.
Dean R, Helps C, Gruffydd Jones TJ and Tasker S (2008). Use of real-time PCR to detect Mycoplasma haemofelis and ‘Candidatus Mycoplasma haemominutum’ in the saliva and salivary glands of haemoplasma-infected cats. Journal of Feline Medicine and Surgery 10: 413417.
DeBuysscher E and Berman D (1980). Secretory immune response in intestinal mucosa and salivary gland after experimental infection of pigs with transmissible gastroenteritis virus. American Journal of Veterinary Research 41: 12141220.
DeBuysscher E and Dubois R (1978). Detection of IgA anti-Escherichia coli plasma cells in the intestine and salivary glands of pigs orally and locally infected with E. coli. Advances in Experimental Medicine and Biology 107: 593600.
Delima AJ and Van Dyke TE (2003). Origin and function of the cellular components in gingival crevice fluid. Periodontology 2000 31: 5576.
Duncan A, Maggi R and Breitschwerdt E (2007). Bartonella DNA in dog saliva. Emerging Infectious Diseases 13: 19481950.
Eblé P, Bouma A, de Bruin M, van Hemert-Kluitenberg F, Van Oirschot J and Dekker A (2004). Vaccination of pigs two weeks before infection significantly reduces transmission of foot-and-mouth disease virus. Vaccine 22: 13721378.
Ellison SA, Mashimo PA and Mandel ID (1960). Immunochemical studies of human saliva. Journal of Dental Research 39: 892898.
Fekadu M, Shaddock J and Baer G (1982). Excretion of rabies virus in the saliva of dogs. Journal of Infectious Diseases 145: 715719.
Figueroa F, Ohlbaum A and Contreras G (1973). Neutralizing antibody response in bovine serum and nasal and salivary secretions after immunization with live or inactivated food-and-mouth disease virus. Infection and Immunity 8: 296298.
Formenty P, Leroy EM, Epelboin A, Libama F, Lenzi M, Sudeck H, Yaba P, Allarangar Y, Boumandouki P, Nkounkou VB, Drosten C, Grolla A, Feldmann H and Roth C (2006). Detection of Ebola virus in oral fluid specimens during outbreaks of Ebola virus hemorrhagic fever in the Republic of Congo. Clinical Infectious Diseases 42: 15211526.
Frankova V and Sixtova E (1987). Specific IgM antibodies in the saliva of mumps patients. Acta Virologica 31: 357364.
Frerichs R, Silarug N, Eskes N, Pagcharoenpol P, Rodklai A, Thangsupachai S and Wongba C (1994). Saliva-based HIV-antibody testing in Thailand. AIDS 8: 885894.
Furuta Y, Aizawa H, Ohtani F, Sawa H and Fukuda S (2004). Varicella–Zoster virus DNA level and facial paralysis in Ramsay Hunt syndrome. Annals of Otology, Rhinology, and Laryngology 113: 700705.
Fylkesnes K and Kasumba K (1998). The first Zambian population-based HIV survey: saliva-based testing is accurate and acceptable. AIDS 12: 540541.
Genco R, Yecies L and Karush F (1969). The immunoglobulins of equine colostrum and parotid fluid. Journal of Immunology 103: 437444.
German A, Hall E and Day M (1998). Measurement of IgG, IgM and IgA concentrations in canine serum, saliva, tears and bile. Veterinary Immunology and Immunopathology 64: 107121.
Gomes-Keller M, Tandon R, Riondato F, Gönczi M, Meli M, Hofmann-Lehmann R and Lutz H (2006a). Detection of feline leukemia virus RNA in saliva from naturally infected cats and correlation of PCR results with those of current diagnostic methods. Journal of Clinical Microbiology 44: 916922.
Gomes-Keller M, Tandon R, Gönczi E, Meli M, Hofmann-Lehmann R and Lutz H (2006b). Shedding of feline leukemia virus RNA in saliva is a consistent feature in viremic cats. Veterinary Microbiology 112: 1121.
Grönblad EA and Mäkelä O (1986). Salivary and serum antibodies in patients with Yersinia enterocolitica infection. Microbial Pathogenesis 1: 565571.
Groopman J, Salahuddin S, Sarngadharan M, Markham P, Gonda M, Sliski A and Gallo R (1984). HTLV-III in saliva of people with AIDS-related complex and healthy homosexual men at risk for AIDS. Science 226: 447449.
Habel RE (1975). Ruminant digestive system. In: Getty R, Rosenbaum CE, Ghoshal NG and Hillman D (eds) The Anatomy of the Domestic Animals, Vol. 1, 5th edn.Philadelphia, PA: Saunders, pp. 861915.
Harley R, Gruffydd-Jones T and Day M (1998). Determination of salivary and serum immunoglobulin concentrations in the cat. Veterinary Immunology and Immunopathology 65: 99112.
Heddle R and Rowley D (1975). Dog immunoglobulins. I. immunochemical characterization of dog serum, parotid saliva, colostrum, milk and small bowel fluid. Immunology 29: 185195.
Herath HM (2003). Early diagnosis of typhoid fever by the detection of salivary IgA. Journal of Clinical Pathology 56: 694698.
Hochman N, Zakay-Rones Z, Shohat H, Ever-Hadani P, Ehrlich J, Schlesinger M and Morag A (1998). Antibodies to cytomegalo and Epstein–Barr viruses in human saliva and gingival fluid. The New Microbiologica 21: 131139.
Holtkamp D, Rotto H and Garcia R (2007). The economic cost of major health challenges in large U.S. swine production systems. In: Proceedings of the 2007 American Association of Swine Veterinarians Annual Meeting, Orlando, Florida, pp. 8589.
Horner M (1976). The passage of drugs into horse saliva and the suitability of saliva for pre-race testing. British Journal of Sports Medicine 10: 133140.
Hurlimann J and Darling H (1971). In vitro synthesis of immunoglobulin-A by salivary glands from animals of different species. Immunology 21: 101111.
Kanter F and Appleton J (1940). Antibacterial effect of saliva on tubercle bacilli. Journal of Dental Research 19: 279280.
Kasempimolporn S, Saengseesom W, Lumlertdacha B and Sitprija V (2000). Detection of rabies virus antigen in dog saliva using a latex agglutination test. Journal of Clinical Microbiology 38: 30983099.
Kinder A, Carter S, Allan J, Marshall-Clarke S and Craing P (1992). Salivary and serum antibodies in experimental canine taeniasis. Veterinary Parasitology 41: 321327.
Kraus F and Konno J (1963). Antibodies in saliva. Annals of the New York Academy of Sciences 106: 311329.
Lewis M, Wright K, Lafrado L, Shanker P, Palumbo N, Lemoine E and Olsen R (1987). Saliva as a source of feline leukemia virus antigen for diagnosis of disease. Journal of Clinical Microbiology 25: 13201322.
Litt DJ, Samuel D, Duncan J, Harnden A, George RC and Harrison TG (2006). Detection of anti-pertussis toxin IgG in oral fluids for use in diagnosis and surveillance of Bordetella pertussis infection in children and young adults. Journal of Medical Microbiology 55: 12231228.
Llena-Puy C (2006). The role of saliva in maintaining oral health and as an aid to diagnosis. Medicina Oral, Patología Oral y Cirugía Bucal 11: E449455.
Loftager M, Eriksen L and Nielsen R (1993). Antibodies against Actinobacillus pleuropneumoniae serotype 2 in mucosal secretions and sera of infected pigs as demonstrated by an enzyme-linked immunosorbent assay. Research in Veterinary Science 54: 5762.
Lutz H and Jarrett O (1987). Detection of feline leukemia virus infection in saliva. Journal of Clinical Microbiology 25: 827831.
Luzza F, Maletta M, Imeneo M, Doldo P, Marasco R, Biancone L and Pallone F (1995). Salivary specific IgG is a sensitive indicator of the humoral immune response to Helicobacter pylori. FEMS Immunology and Medical Microbiology 10: 281283.
Madonia JV, Bahn AN and Calandra JC (1966). Salivary excretion of Coxsackie b-1 virus in rabbits. Applied Microbiology 14: 394396.
Mandel ID (1990). The diagnostic uses of saliva. Journal of Oral Pathology and Medicine 19: 119125.
Mandel ID (1993). Salivary diagnosis: promises, promises. Annals of the New York Academy of Sciences 694: 110.
Martin N (1969). The immunoglobulins: a review. Journal of Clinical Pathology 22: 117131.
Mestecky J (1987). The common mucosal immune system and current strategies for induction of immune responses in external secretions. Annals of the New York Academy of Sciences 7: 265276.
Mestecky J (1993). Saliva as a manifestation of the common mucosal immune system. Annals of the New York Academy of Sciences 694: 184194.
Michaels P (1901). Saliva as an aid in the detection of diathetic diseases. Dental Digest 7: 105110.
Moe CL, Sair A, Lindesmith L, Estes MK and Jaykus LA (2004). Diagnosis of Norwalk virus infection by indirect enzyme immunoassay detection of salivary antibodies to recombinant Norwalk virus antigen. Clinical and Diagnostic Laboratory Immunology 11: 10281034.
Morgan C and Gellhorn A (1947). Detection of drugs in horse saliva. Analytical Chemistry 19: 806808.
Morrier J and Barsotti O (1990). [Secretary IgA and the oral cavity: general review] <original> IgA sécrétoire et cavité buccale: revue générale. Actualités Odonto-Stomatologiques 44: 349364.
Moss DM, Montgomery JM, Newland SV, Priest JW and Lammie PJ (2004). Detection of cryptosporidium antibodies in sera and oral fluids using multiplex bead assay. The Journal of Parasitology 90: 397404.
Nair P and Schroeder H (1986). Duct associated lymphoid tissues (DALT) of minor salivary glands and mucosal immunity. Immunology 57: 171180.
Navazesh M (1993). Methods for collecting saliva. Annals of the New York Academy of Sciences 694: 7277.
Newcomb RW, Ishizaka K and DeVald BL (1969). Human IgG and IgA diphtheria antitoxins in serum, nasal fluids and saliva. Journal of Immunology 103: 215224.
Nigatu D, Samuel D, Cohen B, Cumberland P, Lemma E, Brown D and Nokes J (2008). Evaluation of a measles vaccine campaign in Ethiopia using oral-fluid antibody surveys. Vaccine 26: 47694774.
Nightingale S (1995). From the food and drug administration: oral fluid specimen test system for HIV-1 approved. Journal of the American Medical Association 273: 613.
Ohuma E, Okiro E, Bett A, Abwao J, Were S, Samuel D, Vyse A, Gay N, Brown D and Nokes D (2009). Evaluation of a measles vaccine campaign by oral-fluid surveys in a rural Kenyan district: interpretation of antibody prevalence data using mixture models. Epidemiology and Infection 137: 227233.
Oliveira S, Siqueira M, Brown D, Camacho L, Faillace T and Cohen B (1998). Salivary diagnosis of measles for surveillance: a clinic-based study in Niterói, state of Rio de Janeiro, Brazil. Transactions of the Royal Society of Tropical Medicine and Hygiene 92: 636638.
Parida S, Anderson J, Cox S, Barnett P and Paton D (2006). Secretory IgA as an indicator of oro-pharyngeal foot-and-mouth disease virus replication and as a tool for post vaccination surveillance. Vaccine 24: 11071116.
Petraityte R, Jin L, Hunjan R, Razanskiene A, Zvirbliene A and Sasnauskas K (2007). Use of Saccharomyces cerevisiae-expressed recombinant nucleocapsid protein to detect Hantaan virus-specific immunoglobulin G (IgG) and IgM in oral fluid. Clinical and Vaccine Immunology 14: 16031608.
Pinho RT, Pedrosa RC, Costa-Martins P and Castello-Branco LR (1999). Saliva ELISA: a method for the diagnosis of chronic Chagas disease in endemic areas. Acta Tropica 72: 3138.
Poli A, Giannelli C, Pistello M, Zaccaro L, Pieracci D, Bendinelli M and Malvaldi G (1992). Detection of salivary antibodies in cats infected with feline immunodeficiency virus. Journal of Clinical Microbiology 30: 20382041.
Pollaci G and Ceraulo S (1909). [The agglutinating properties of several body fluids during Malta fever] <original> Das agglutinationsvermögen einiger körperflüssigkeiten beim Mediterranfieber. Centralblatt für Bakteriologie, Parasitenkunde und Infektionskrankheiten (I). Abt Originale 52: 268275.
Prasad SR, Yergolkar PN, Walhekar BD and Dandawate CN (1994). Virus specific IgM, IgG and IgA antibodies in serum and saliva of Japanese encephalitis patients. Indian Journal of Pediatrics 61: 109110.
Prickett J, Cutler S, Kinyon J, Naberhaus N, Stensland W, Yoo KJ and Zimmerman JJ (2010). Stability of PRRSV diagnostic targets in swine oral fluid samples. Journal of Swine Health and Production (in press).
Prickett J, Simer R, Christopher-Hennings J, Yoon K-J, Evans RB and Zimmerman J (2008a). Detection of porcine reproductive and respiratory syndrome virus infection in porcine oral fluid samples: a longitudinal study under experimental conditions. Journal of Veterinary Diagnostic Investigation 20: 156163.
Prickett J, Simer R, Yoon K-J, Kim W-I and Zimmerman J (2008b). Surveillance of commercial growing pigs for PRRSV and PCV2 infections using pen-based oral fluid samples: A pilot study. Journal of Swine Health and Production 16: 8691.
Ramsay M, Brugha R and Brown D (1997). Surveillance of measles in England and Wales: implications of a national saliva testing programme. Bulletin of the World Health Organization 75: 515521.
Renter D, Visser A, McFall M, Smith D, Berg J, Silasi L, Berezowski J and Sorensen O (2004). Rapid pen-level surveillance of E. coli O157:H7 in finished feedlot cattle. Animal Health Forum 9: 3.
Rothman R and Kalish B (2009). Update on emerging infections: news from the Centers for Disease Control and Prevention. False-positive oral fluid rapid HIV tests – New York City, 2005–2008. Annals of Emergency Medicine 53: 151156.
Schaefer E (1990). AIDS testing trail approved for saliva test. Nature 346: 6287.
Scicchitano R, Sheldrake RF and Husband AJ (1986). Origin of immunoglobulins in respiratory tract secretion and saliva of sheep. Immunology 58: 315321.
Scott DA, Coulter WA and Larney PJ (1997). Oral shedding of herpes simplex virus type 1: a review. Journal of Oral Pathology and Medicine 26: 441447.
Shackleford J and Wilborn WH (1968). Structural and histochemical diversity in mammalian salivary glands. The Alabama Journal of Medical Sciences 5: 180203.
Sisson S (1975a). Equine digestive system. In: Getty R, Rosenbaum CE, Ghoshal NG and Hillman D (eds) The Anatomy of the Domestic Animals, Vol. 1, 5th edn.Philadelphia, PA: Saunders, pp. 454497.
Sisson S (1975b). Porcine digestive system. In: Getty R, Rosenbaum CE, Ghoshal NG and Hillman D (eds) The Anatomy of the Domestic Animals, Vol. 1, 5th edn.Philadelphia, PA: Saunders, pp. 12691282.
Smith D, Gray T, Moxley R, Younts-dahl S, Blackford M, Hinkley S, Hungerford J, Milton C and Klopfenstein T (2004). A diagnostic strategy to determine the shiga toxin-producing Escherichia coli 0157 status of pens of feedlot cattle. Epidemiology and Infection 132: 297302.
Smith D, Moxley R, Clowser S, Folmer J, Hinkley S, Erickson G and Klopfenstein T (2005). Use of rope devices to describe and explain the feedlot ecology of Salmonella by time and place. Foodborne Pathogens and Disease 2: 6169.
Smith W, Dawson A, Wells P and Burrells C (1975). Immunoglobulin concentrations in ovine body fluids. Research in Veterinary Science 19: 189194.
South M, Cooper M, Wollheim F, Hong R and Good R (1966). The IgA system. I. Studies of the transport and immunochemistry of IgA in the saliva. Journal of Experimental Medicine 123: 615627.
Stallknecht D, Howerth E, Reeves C and Seal B (1999). Potential for contact and mechanical vector transmission of vesicular stomatitis virus New Jersey in pigs. American Journal of Veterinary Research 60: 4348.
Stanford K, Bach S, Marx T, Jones S, Hansen J, Wallins G, Zahiroddini H and McAllister T (2005). Monitoring Escherichia coli O157:H7 in inoculated and naturally colonized feedlot cattle and their environment. Journal of Food Protection 68: 2633.
Tabak LA (2007). Point-of-care diagnostics enter the mouth. Annals of the New York Academy of Sciences 1098: 714.
Tomasi T and Zigelbaum S (1963). The selective occurrence of gamma-1A globulins in certain body fluids. Journal of Clinical Investigation 42: 15521560.
Waldman RH, Mann JJ and Kasel JA (1968). Influenza virus neutralizing antibody in human respiratory secretions. Journal of Immunology 100: 8085.
Wang WK, Chen SY, Liu IJ, Chen YC, Chen HL, Yang CF, Chen PJ, Yeh SH, Kao CL, Huang LM, Hsueh PR, Wang JT, Sheng WH, Fang CT, Hung CC, Hsieh SM, Su CP, Chiang WC, Yang JY, Lin JH, Hsieh SC, Hu HP, Chiang YP, Wang JT, Yang PC and Chang SC (2004). Detection of SARS-associated coronavirus in throat wash and saliva in early diagnosis. Emerging Infectious Diseases 10: 12131219.
Ward RL, Pax KA, Sherwood JR, Young EC, Schiff GM and Bernstein DI (1992). Salivary antibody titers in adults challenged with a human rotavirus. Journal of Medical Virology 36: 222225.
Wheatcroft M (1957). A comparative study of human serum and salivary antibody titers in cases of Brucella melitensis infections. Journal of Dental Research 36: 112117.
Wiggs RB and Lobprise HB (1997). Oral anatomy and physiology. In: Veterinary Dentristy, Principles and Practice. Philadelphia, PA: Lippincott-Raven Publishers, pp. 5586.
Willi B, Boretti FS, Baumgartner C, Tasker S, Wenger B, Cattori V, Meli ML, Reusch CE, Lutz H and Hofmann-Lehmann R (2006). Prevalence, risk factor analysis, and follow-up of infections caused by three feline hemoplasma species in cats in Switzerland. Journal of Clinical Microbiology 44: 961969.
Yamamoto J, Sparger E, Ho E, Andersen P, O'Connor T, Mandell C, Lowenstine L, Munn R and Pedersen N (1988). Pathogenesis of experimentally induced feline immunodeficiency virus infection in cats. American Journal of Veterinary Research 49: 12461258.
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