Hostname: page-component-7dd5485656-wjfd9 Total loading time: 0 Render date: 2025-10-31T08:57:59.728Z Has data issue: false hasContentIssue false
  • English
  • Français

Adipose tissue metabolism and cell size: variation between subcutaneous sites and the effect of copper supplementation

Published online by Cambridge University Press:  02 September 2010

P. A. Sinnett-Smith  and
J. A. Woolliams
P. A. Sinnett-Smith
Affiliation:
AFRC Institute of Animal Physiology and Genetics Research†, Edinburgh Research Station, Roslin, Midlothian EH25 9PS
J. A. Woolliams
Affiliation:
AFRC Institute of Animal Physiology and Genetics Research†, Edinburgh Research Station, Roslin, Midlothian EH25 9PS
Get access

Abstract

Adipose tissue cell volume, lipolytic rates, Iipoprotein lipase (LPL) and acetyl CoA carboxylase (ACC) activities were determined in biopsy samples taken from the back, shoulder, rump and groin subcutaneous fat depots of 17-month-old female sheep (no. = 24). Half of the sheep had received a copper (Cu) supplement at 6 weeks of age. Biopsy samples were taken after fasting overnight.

Differences in cell volume and ACC activity were apparent between sites. Supplementation with Cu at 6 weeks of age led to a proportional increase of between 0·24 and 0·45 in adipose cell volume and a proportional increase in lipolysis which varied from 0·70 to 2·27 depending on site and hormone addition. Correlations between sites for cell volume and lipolytic activity were not high in general but those for ACC and LPL activities were higher (for ACC r = 0·40 to 0·79). Overall the best correlations were between the back and rump sites. Correlations between the various metabolic measurements and cell volume were not apparent within treatment groups.

Information

Type
Research Article
Information
Animal Production , Volume 45 , Issue 1 , August 1987 , pp. 75 - 80
Copyright
Copyright © British Society of Animal Science 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

Brownsey, R. W., Hughes, W. A. and Denton, R. M. 1979. Adrenaline and the regulation of acetyl-coenzyme A caroboxylase in rat epididymal adipose tissue. Biochemal Journal 184: 23–32.CrossRefGoogle ScholarPubMed
Di Girolamo, M., Medlinger, S. and Fertig, J. W. 1971. A simple method to determine fat cell size and number in four mammalian species. American Journal of Physiology 221: 850–858.CrossRefGoogle ScholarPubMed
Fields, M., Ferretti, R. J., Smith, J. C. and Reiser, S. 1983. Effect of copper deficiency on metabolism and mortality in rats fed sucrose or starch diets. Journal of Nutrition 113: 1335–1345.CrossRefGoogle ScholarPubMed
Flint, D. J., Sinnett-Smith, P. A., Clegg, R. A. and Vernon, R. G. 1979. Role of insulin receptors in the changing metabolism of adipose tissue during pregnancy and lactation in the rat. Biochemical Journal 182: 421–427.CrossRefGoogle ScholarPubMed
Honnor, R. C. and Saggerson, E. D. 1980. Altered lipolytic response to glucagon and adenosine deaminase i n adipocytes from starved rats. Biochemical Journal 188: 757–761.CrossRefGoogle Scholar
Ingle, D. L., Bauman, D. E. and Garrigus, U. S. 1972a. Lipogenesis in the ruminant: in vitro study of tissue sites, carbon source and reducing equivalent generation for fatty acid synthesis. Journal of Nutrition 102: 609–616.CrossRefGoogle ScholarPubMed
Ingle, D. L., Bauman, D. E. and Garrigus, U. S. 1972b. Lipogenesis in the ruminant: in vivo site of fatty acid synthesis in sheep. Journal of Nutrition 102: 617–624.CrossRefGoogle ScholarPubMed
Ingle, D. L., Bauman, D. E., Mellenberger, R. W. and Johnson, D. E. 1973. Lipogenesis in the ruminant: effect of fasting and refeeding on fatty acid synthesis and enzymatic activity of sheep adipose tissue. Journal of Nutrition 103: 1479–1488.CrossRefGoogle ScholarPubMed
Kempster, A. J., Cook, G. L. and Grantley-Smith, M. 1986. National estimates of the body composition of British cattle, sheep and pigs with special reference t o trends in fatness: a review. Meat Science 17: 107–138.CrossRefGoogle Scholar
Leat, W. M. F. 1974. The control of fat absorption, deposition and mobilisation in farm animals. In Meat Animals: Growth and Productivity (eds. Lister, D., Rhodes, D. N., Fowler, V. R. and Fuller, M. F.), pp. 177–193. Plenum Press, New York.Google Scholar
Meddis, R. 1980. Unified analysis of variance by ranks. British Journal of Mathematical and Statistical Psychology 33: 84–98.CrossRefGoogle Scholar
Moore, J. H., Christie, W. W., Braude, R. and Mitchell, K. G. 1969. The effect of dietary copper on the fatty acid composition and physical properties of pig adipose tissues. British Journal of Nutrition 23: 281–287.CrossRefGoogle ScholarPubMed
Pond, C. M. 1984. Physiological and ecological importance of energy storage in the evolution of lactation: evidence for a common pattern of anatomical organisation of adipose tissue in mammals. In Physiological Strategies in Lactation (ed. Peaker, M., Knight, C. H. and Vernon, R. G.), pp. 1–32. Academic Press, London.Google Scholar
Thornton, R. F., Hood, R. L., Rowe, R. W. D. and Jones, P. N. 1983. The cellular and metabolic organisation of ovine subcutaneous adipose tissue. Australian Journal of Biological Science 36: 41–48.Google Scholar
Tume, R. K., Thornton, R. F. and Johnson, G. W. 1983. Lipoprotein lipase of sheep and rat adipose tissues. Australian Journal of Biological Science 36: 41–48.CrossRefGoogle ScholarPubMed
Vernon, R. G. 1980. Lipid metabolism in the adipose tissue of ruminant animals. Progress in Lipid Research 19: 23–106.CrossRefGoogle ScholarPubMed
Vernon, R. G., Clegg, R. A. and Flint, D. J. 1981. Metabolism of sheep adipose tissue during pregnancy and lactation. Biochemical Journal 200: 307–314.CrossRefGoogle ScholarPubMed
Vernon, R. G. and Finley, E. 1985. Regulation of lipolysis during pregnancy and lactation in sheep. Biochemical Journal 230: 651–656.CrossRefGoogle ScholarPubMed
Vezhinet, A. and Prud'hon, M. 1975. Evolution of various adipose deposits in growing rabbits and sheep. Animal Production 20: 363–370.CrossRefGoogle Scholar
Wang, C. S. and Smith, R. L. 1975. Lowry determination of protein in the presence of Triton X-100. Analytical Biochemistry 63: 414–417.CrossRefGoogle ScholarPubMed
Wiener, G. 1987. The genetics of copper metabolism in animals and man. In Copper in Animals and Man Vol. 1 (ed. Howell, J. McC. and Gawthorne, J. M.), CRC Press, Florida. In press.Google Scholar
Woolliams, C., Suttle, N. F., Woolliams, J. A., Jones, D. G. and Wiener, G. 1986a. Studies on lambs from lines genetically selected for low and high copper status. 1. Differences in mortality. Animal Production 43: 293–301.Google Scholar
Woolliams, J. A., Suttle, N. F., Woolliams, C., Jones, D. G. and Wiener, G. 1986b. Studies on lambs from lines genetically selected for low and high copper status. 2. Incidence of hypocuprosis on improved hill pasture. Animal Production 43: 303–317.Google Scholar