Hostname: page-component-7dd5485656-bvgqh Total loading time: 0 Render date: 2025-10-31T01:00:27.684Z Has data issue: false hasContentIssue false
  • English
  • Français

Traceability of grass-feeding in young beef using carotenoid pigments in plasma and adipose tissue

Published online by Cambridge University Press:  13 March 2007

E. Serrano ,
S. Prache ,
B. Chauveau-Duriot ,
J. Agabriel  and
D. Micol
E. Serrano*
Affiliation:
Unité de Recherches sur les Herbivores, INRA Clermont-Theix 63122 St-Genès-Champanelle, France
S. Prache
Affiliation:
Unité de Recherches sur les Herbivores, INRA Clermont-Theix 63122 St-Genès-Champanelle, France
B. Chauveau-Duriot
Affiliation:
Unité de Recherches sur les Herbivores, INRA Clermont-Theix 63122 St-Genès-Champanelle, France
J. Agabriel
Affiliation:
Unité de Recherches sur les Herbivores, INRA Clermont-Theix 63122 St-Genès-Champanelle, France
D. Micol
Affiliation:
Unité de Recherches sur les Herbivores, INRA Clermont-Theix 63122 St-Genès-Champanelle, France
*
†E-mail: melandrera@yahoo.es
Get access

Abstract

The objective of the present study was to test the reliability of the analysis method of the reflectance spectrum of fat in the zone of light absorption by carotenoid pigments developed in lambs to trace grass feeding in calves. Six feeding treatments were compared in suckling calves: ad libitum cocksfoot hay plus ad libitum concentrate or half level of ad libitum concentrate (HH and HL), ad libitum cut grass plus ad libitum concentrate or half level of ad libitum concentrate (GH and GL), ad libitum highland grazed grass plus ad libitum concentrate (PH); ad libitum medium quality cocksfoot hay plus dam's milk (C0). Groups HH, HL, GH, GL and PH were slaughtered at approximately 10 months of age, after 150 days of experimental feeding diet. C0 calves were slaughtered at the beginning of the experiment at approximately 6 months of age. The concentration of carotenoids was analysed by HPLC on diets and calves' plasma at slaughter. The reflectance spectrum of perirenal and subcutaneous fat were measured at slaughter and expressed as AVI units. Plasma carotenoid content was higher (P<0·05) in PH and GL calves than in HH, HL and GH calves. There was an effect (P<0·05) of feeding treatment and measurement site on AVI. Values obtained for perirenal fat were higher (P<0·05) than those obtained for subcutaneous fat. For plasma carotenoid content and for AVI values in both fat tissues there was no overlapping between hay-fed calves on the one hand and GL or grazing calves on the other hand. However, there was some overlapping between hay-fed calves and GH calves. For plasma carotenoid content, values for C0 calves overlapped with values of GH and GL calves. For both fat tissues AVI values of C0 calves overlapped with values of HH, HL, GH and GL calves. AVI of cut grass fed calves (GH and GL groups) increased linearly (P<0·05) with average daily grass intake for both tissues considered. AVI in subcutaneous fat tissue decreased linearly (P<0·05) with average daily concentrate intake but this relation was not significant for AVI in perirenal fat (P>0·05). Plasma carotenoid concentration and the reflectance spectrum of the fat in the zone of light absorption by carotenoid pigments could be used to trace grass feeding in calves. Results obtained in this study suggest that different fatty tissues could differ in their reliability and interest for traceability of grass-feeding, depending on the animal age and production system.

Keywords

beef cattlecarotenoidsgrazingtracer techniques

Information

Type
Research Article
Information
Animal Science , Volume 82 , Issue 6 , December 2006 , pp. 909 - 918
Copyright
Copyright © British Society of Animal Science 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

Arana, A., Soret, B., Mendizabal, J. A., Corroza, M., Eguinoa, P., Purroy, A. (1998) Changes in adipose tissue accumulation in Rasa Aragonesa breed lambs during growth and fattening. Animal Science 66: 409–413.CrossRefGoogle Scholar
Cardinault, N., Doreau, M., Poncet, C., Nozière, P. (2006) Digestion and absorption of carotenoids in sheep given fresh red clover. Animal Science 82: 49–55.CrossRefGoogle Scholar
Chauveau-Duriot, B., Thomas, D., Portelli, J., Doreau, M. (2005) Effet du mode de conservation sur la teneur en caroténoïdes des fourrages. Rencontres Recherches Ruminants 12: 117.Google Scholar
Commission International de l'Eclairage. 1986. Colorimetry, 2nd edition. CIE publication no. 15.2. CIE, Vienna.Google Scholar
Cody, R. P., Smith, J. K. (1991) Applied statistics and the SAS programming language. Prentice Hall, New Jersey.Google Scholar
Cornu, A., Kondjoyan, N., Begnaud, F., Micol, D., Renou, J. P., Berdagué, J. L. (2001) Les terpènes des viandes, traceurs de l'alimentation et de l'origine géographique des animaux. Rencontres Recherches Ruminants 8: 61.Google Scholar
Coulon, J. B., Priolo, A. (2002) Influence of forage feeding on the composition and organoleptic properties of meat and dairy products: bases for a “terroir” effect. Proceedings of the 19th general meeting of the European Grassland Federation (ed. Durand, J. L., Emile, J. C., Huyghe, C., Lemaire, G.), pp. 513–524, La Rochelle,France.Google Scholar
French, P., O'Riordan, E. G., Monahan, F. J., Caffrey, P. J., Vidal, M., Mooney, M. T., Troy, D. J., Moloney, A. P. (2000) Meat quality of steers finished on autumn grass, grass silage, or concentrate-based diets. Meat Science 56: 173–180.CrossRefGoogle ScholarPubMed
Kirton, A. H., Crane, B., Paterson, D. J., Clare, N. T. (1975) Yellow fat in lambs caused by carotenoid pigmentation. New Zealand Journal of Agricultural Research 18: 267–272.CrossRefGoogle Scholar
Knight, T. W., Waghorn, G. C. (1992) Absorption and deposition of β-carotene in beef and dairy cattle. In Milkfat flavour forum. New Zealand Dairy Research Institute, Palmerston, NZ, 55–61.Google Scholar
Knight, T. W., Death, A. F. (1999) Effects of oral and injected vitamin A (retinol) supplements on liver vitamin A and plasma carotenoid and cholesterol concentrations in cattle. Animal Science 69: 607–612.CrossRefGoogle Scholar
Knight, T. W., Death, A. F., Lambert, M. G., McDougall, D. B. (2001) The rate of reduction in carotenoid concentration in fat of steers fed a low carotenoid ration, and the role of increasing carcass fatness. Australian Journal of Agricultural Research 52: 1023–1032.CrossRefGoogle Scholar
Lyan, B., Azaïs-Braesco, V., Cardinault, N., Tyssandier, V., Borel, P., Alexandre-Gouabau, M. C., Grolier, P. (2001) Simple method for clinical determination of 13 carotenoids in human plasma using an isocratic high-performance liquid chromatographic method. Journal of Chromatography B 751: 297–303.CrossRefGoogle ScholarPubMed
Martin, B., Ferlay, A., Pradel, P., Rock, E., Grolier, D., Dupont, D., Gruffat, J. M., Besle, N., Ballot, Y., Chilliard, Y., Coulon, J. B. (2002) Variabilité de la teneur des laits en constituants d'intérêt nutritionnel selon la nature des fourrages consommés par les vaches laitières. Rencontres Recherches Ruminants 9: 347–350.Google Scholar
Martin, B., Cornu, A., Kondjoyan, N., Ferlay, A., Verdier-Metz, I., Pradel, P., Rock, E., Chilliard, Y., Coulon, J. B., Berdagué, J. L. (2005) Milk indicators for recognizing the types of forages eaten by dairy cows. Proceedings of the European Association of Animal Production,publication no. 112. Bled,Slovenia,127–136.CrossRefGoogle Scholar
Nozière, P., Grolier, P., Durand, D., Ferlay, A., Pradel, P., Martin, B. (2006) Variations in carotenoids, fat-soluble micronutrients, and color in cows' plasma and milk following change in forage and feeding level. Journal of Dairy Science 89: 2634–2648.CrossRefGoogle ScholarPubMed
Patterson, D. S. P. (1965) The association between depot fat mobilization and the presence of xanthophyll in the plasma of normal sheep. Journal of Agricultural Science, Cambridge 64: 273–278.CrossRefGoogle Scholar
Prache, S., Cornu, A., Berdagué, J. L., Priolo, A. (2005) Traceability of animal feeding diet in the meat and milk of small ruminants. Small Ruminant Research 59: 157–168.CrossRefGoogle Scholar
Prache, S., Priolo, A., Grolier, P. (2003a) Effect of concentrate finishing on the carotenoid content of perirenal fat in grazing sheep: its significance for discriminating grass-fed, concentrate-fed and concentrate-finished grazing lambs. Animal Science 77: 225–233.CrossRefGoogle Scholar
Prache, S., Priolo, A., Grolier, P. (2003b) Persistence of carotenoid pigments in the blood of concentrate-finished grazing sheep: its significance for the traceability of grass feeding. Journal of Animal Science 81: 360–367.CrossRefGoogle ScholarPubMed
Prache, S., Priolo, A., Tournadre, H., Jailler, R., Dubroeucq, H., Micol, D., Martin, B. (2002b) Traceability of grass-feeding by quantifying the signature of carotenoid pigments in herbivores meat, milk and cheese. In Proceedings of the 19th General Meeting of the European Grassland Federation (ed. Durand, J. L., Emile, J. C., Huyghe, C., Lemaire, G.) pp. 592–593La Rochelle,France.Google Scholar
Prache, S., Theriez, M. (1999) Traceability of lamb production systems: carotenoids in plasma and adipose tissue. Animal Science 69: 29–36.CrossRefGoogle Scholar
Priolo, A., Cornu, A., Prache, S., Krogmann, M., Kondjoyan, N., Micol, D., Berdagué, J. L. (2004) Fat volatiles tracers of grass feeding in sheep. Meat Science 66: 475–481.CrossRefGoogle ScholarPubMed
Priolo, A., Prache, S., Micol, D., Agabriel, J. (2002) Reflectance spectrum of adipose tissue to trace grass feeding in sheep: Influence of measurement site and shrinkage time after slaughter. Journal of Animal Science 80: 886–891.CrossRefGoogle ScholarPubMed
Robelin, J. (1986) Growth of adipose tissues in cattle: partitioning between depots, chemical composition and cellularity: a review. Livestock Production Science 14: 349–364.CrossRefGoogle Scholar
Robelin, J. (1978) Répartition des dépôts adipeux chez les bovins selon l'état d'engraissement, le sexe et la race. Bulletin Technique Centre de Recherches Zootechniques et Veterinaires de Theix 34: 31–34.Google Scholar
Robelin, J., Geay, Y. (1975) Estimation de la composition de la carcasse des taurillons à partir de la composition de la 6 ème côte. Bulletin Technique Centre de Recherches Zootechniques et Veterinaires de Theix 22: 41–44.Google Scholar
Rock, C. L. (1997) Carotenoids: biology and treatment. Pharmacology and Therapeutics 75: 185–197.CrossRefGoogle ScholarPubMed
Statistical Analysis Systems Institute (1989) SAS/STAT®. User's guide, version 6, 4th edition. Statistical Analysis Systems Institute Inc., Cary, NC.Google Scholar
Serrano, E., Cornu, A., Kondjoyan, N., Figueredo, G., Agabriel, J., Micol, D. (2006a) Terpene accumulation in muscle and fatty tissues of veal supplemented with essential oils. Journal of Animal and Feed Sciences submitted.Google Scholar
Serrano, E., Pradel, Ph., Roland, J., Dubroeucq, H., Bauchart, D., Hocquette, J. F., Listrat, A., Agabriel, J., Micol, D. (2006b) Young suckling Salers bulls production: effect of type of diet on performances, carcass and muscle characteristics and meat quality. Animal Research submitted.Google Scholar
Swatland, H. J. (1988) Carotene reflectance and the yellowness of bovine adipose tissue measured with a portable fibre-optic spectrophotometer. Journal of the Science of Food and Agriculture 46: 195–200.CrossRefGoogle Scholar
Varela, A., Oliete, B., Moreno, T., Portela, C., Monserrat, L., Carballo, J. A., Sanchez, L. (2004) Effect of pasture finishing on the meat characteristics and intramuscular fatty acid profile of steers of the Rubia Gallega breed. Meat Science 67: 515–522.CrossRefGoogle ScholarPubMed
Vernon, R. G. (1986) The growth and metabolism of adipocytes. In Proceedings of the 43rd Nottingham Easter School in Agricultural Science.Butterworths,London,67–83.CrossRefGoogle Scholar
Williams, P. E. V., Ballet, N., Robert, J. C. (1998) A review of the provision of vitamins for ruminants. Proceedings of the preconference symposium of the Cornell Nutrition Conference1998, 7–37.Google Scholar
Wolter, R. (1988) Alimentation vitaminique Alimentation des bovins, ovins et caprins. Institut National de la Recherche Agronomique, France. pp. 113–120Google Scholar
Yang, A., Brewster, M. J., Lanari, M. C., Tume, R. K. (2002) Effect of vitamin E supplementation on α-tocopherol and β-carotene concentrations in tissues from pasture- and grain-fed cattle. Meat Science 60: 35–40.CrossRefGoogle ScholarPubMed
Yang, A., Larsen, T. W., Tume, R. K. (1992) Carotenoid and retinol concentrations in serum, adipose tissue and liver and carotenoid transport in sheep, goats and cattle. Australian Journal of Agricultural Research 43: 1809–1817.CrossRefGoogle Scholar
Zhou, G. H., Yang, A., Tume, R. K. (1993) A relationship between bovine fat colour and fatty acid composition. Meat Science 35: 205–212.CrossRefGoogle ScholarPubMed