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Effects of temperature on heat-shock responses and survival of two species of marine invertebrates from sub-Antarctic Marion Island

Published online by Cambridge University Press:  26 June 2013

S. Clusella-Trullas*
Affiliation:
Centre for Invasion Biology, Department of Botany and Zoology, Stellenbosch University, Private Bag X1, Stellenbosch 7602, South Africa
L. Boardman
Affiliation:
Department of Conservation Ecology and Entomology, Stellenbosch University, Private Bag X1, Stellenbosch 7602, South Africa
K.T. Faulkner
Affiliation:
Centre for Invasion Biology, Department of Botany and Zoology, Stellenbosch University, Private Bag X1, Stellenbosch 7602, South Africa
L.S. Peck
Affiliation:
British Antarctic Survey, NERC, High Cross, Madingley Road, Cambridge CB3 0ET, UK
S.L. Chown
Affiliation:
School of Biological Sciences, Monash University, VIC 3800, Australia

Abstract

This study examined high temperature survival and heat shock protein 70 (Hsp70) responses to temperature variation for two marine invertebrate species on sub-Antarctic Marion Island. The isopod Exosphaeroma gigas Leach and the amphipod Hyale hirtipalma Dana had the same tolerance to high temperature. The mean upper temperature which was lethal for 50% of the population (upper lethal temperature, ULT50) was 26.4°C for both species. However, the isopod E. gigas showed significant plasticity of ULT50, with a positive response to acclimation. In addition, the isopod had a heat shock response of Hsp70 at all acclimations, and the amount of Hsp70 protein increased significantly from basal levels upon an acute warm exposure after a cold acclimation. By contrast, the amphipod H. hirtipalma showed limited plasticity of ULT50 and no evidence for a heat shock response (failure of three different Hsp70 antibodies to bind to the extracted 70kDa proteins). Overall, these results reflect different flexibility of thermal tolerance of intertidal invertebrate species on Marion Island, with possible variation in the underlying cellular mechanisms, suggesting that warming associated with climate change may result in changes in species assemblage structure in sub-polar environments.

Type
Biological Sciences
Copyright
Copyright © Antarctic Science Ltd 2013 

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Effects of temperature on heat-shock responses and survival of two species of marine invertebrates from sub-Antarctic Marion Island
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