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Isolation and characterization of Antarctic psychrotroph Streptomyces sp. strain INACH3013

  • Paris Leonardo Lavin (a1), Sheau Ting Yong (a2), Clemente M.V.L. Wong (a2) (a3) and Mario De Stefano (a4)

An actinobacterial strain with antimicrobial activity, INACH3013, was isolated from soil collected from Antarctica. The taxonomic status of the isolate was established using a polyphasic approach. The strain was identified as belonging to the genus Streptomyces based on the scanning electron microscopic observation and partial 16S rRNA gene sequence analysis. The sequence analysis revealed that strain INACH3013 is closely related to Streptomyces fildesensis (99.8%), S. beijiangensis (98.1%) and S. purpureus (97.2%). A phylogenetic tree constructed using the partial 16S rRNA gene sequences of strain INACH3013 and closely related strains revealed that INACH3013 fell into the same subclade as S. fildesensis and S. purpureus. Strain INACH3013 was observed to be psychrotolerant, slightly halotolerant (up to 5% NaCl) and capable of inhibiting the growth of seven Gram-negative and eight Gram-positive foodborne pathogens. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of the strain is KJ624755.

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Bentley, S.D., Chater, K.F., Cerdeño-Tárraga, A.M. & 40 others. 2002. Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2). Nature, 417, 141147.
Bérdy, J. 2005. Bioactive microbial metabolites - a personal view. Journal of Antibiotics, 58, 126.
De Souza, M.J., Nair, S., Bharathi, P.L. & Chandramohan, D. 2006. Metal and antibiotic-resistance in psychrotrophic bacteria from Antarctic Marine waters. Ecotoxicology, 15, 379384.
Doroghazi, J.R., Albright, J.C., Goering, A.W., Ju, K.S., Haines, R.R., Tchalukov, K.A., Labeda, D.P., Kelleher, N.L. & Metcalf, W.W. 2014. A roadmap for natural product discovery based on large-scale genomics and metabolomics. Nature chemical biology, 10, 963968.
Edwards, J.A. & Smith, R.I.L. 1988. Photosynthesis and respiration of Colobanthus quitensis and Deschampsia antarctica from the Maritime Antarctic. British Antarctic Survey Bulletin, No. 81, 4363.
Encheva-Malinova, M., Stoyanova, M., Avramova, H., Pavlova, Y., Gocheva, B., Ivanova, I. & Moncheva, P. 2014. Antibacterial potential of streptomycete strains from Antarctic soils. Biotechnology & Biotechnological Equipment, 28, 721727.
Gerber, N.N. & Lechevalier, H.A. 1965. Geosmin, an earthly-smelling substance isolated from actinomycetes. Applied Microbiology, 13, 935938.
Hernández, J., Stedt, J., Bonnedahl, J., Molin, Y., Drobni, M., Calisto-Ulloa, N., Gomez-Fuentes, C., Astorga-Espana, M.S., Gonzalez-Acuna, D., Waldenstrom, J., Blomqvist, M. & Olsena, B. 2012. Human-associated extended-spectrum β-lactamase in the Antarctic. Applied and Environmental Microbiology, 78, 20562058.
Jiang, C. & Xu, L. 1993. Actinomycete diversity in unusual habitats. Actinomycetes, 4, 4757.
Kennedy, A.C. 1999. Bacterial diversity in agroecosystems. Agriculture Ecosystems & Environment, 74, 6576.
Kharel, M.K., Shepherd, M.D., Nybo, S.E., Smith, M.L., Bosserman, M.A. & Rohr, J. 2010. Isolation of Streptomyces species from soil. Current Protocols in Microbiology, 10, 10.1002/9780471729259.mc10e04s19.
Lam, K.S. 2006. Discovery of novel metabolites from marine actinomycetes. Current Opinion in Microbiology, 9, 245251.
Larsen, H. 1986. Halophilic and halotolerant microorganisms – an overview and historical perspective. FEMS Microbiology Letters, 39, 37.
Lazzarini, A., Cavaletti, L., Toppo, G. & Marinelli, F. 2001. Rare genera of actinomycetes as potential producers of new antibiotics. Antonie van Leeuwenhoek, 78, 399405.
Li, J., Tian, X.P., Zhu, T.J., Yang, L.L. & Li, W.J. 2011. Streptomyces fildesensis sp. nov., a novel streptomycete isolated from Antarctic soil. Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology, 100, 537543.
Li, W.J., Zhang, L.P., Xu, P., Cui, X.L., Lu, Z.T., Xu, L.H. & Jiang, C.L. 2002. Streptomyces beijiangensis sp. nov., a psychrotolerant actinomycete isolated from soil in China. International Journal of Systematic and Evolutionary Microbiology, 52, 16951699.
Miller, R.V., Gammon, K. & Day, M.J. 2009. Antibiotic resistance among bacteria isolated from seawater and penguin fecal samples collected near Palmer Station, Antarctica. Canadian Journal of Microbiology, 55, 3745.
Moncheva, P., Tishkov, S., Dimitroval, N., Chipeva, V., Antonova-Nikolova, S. & Bogatzevska, N. 2002. Characteristics of soil actinomycetes from Antarctica. Journal of Culture Collections, 3, 314.
Okoro, C.K., Brown, R., Jones, A.L., Andrews, B.A., Asenjo, J.A., Goodfellow, M. & Bull, A.T. 2009. Diversity of culturable actinomycetes in hyper-arid soils of the Atacama Desert, Chile. Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology, 95, 121133.
Perron, G.G., Whyte, L., Turnbaugh, P.J., Goordial, J., Hanage, W.P., Dantas, G. & Desai, M.M. 2015. Functional characterization of bacteria isolated from ancient Arctic soil exposes diverse resistance mechanisms to modern antibiotics. PLoS ONE, 10, 10.1371/journal.pone.0069533.
Poirel, L., Corvec, S., Rapoport, M., Mugnier, P., Petroni, A., Pasteran, F., Faccone, D., Galas, M., Drugeon, H., Cattoir, V. & Nordmann, P. 2007. Identification of the novel narrow-spectrum β-lactamase SCO-1 in Acinetobacter spp. from Argentina. Antimicrobial Agents and Chemotherapy, 51, 21792184.
Ratkowsky, D.A., Lowry, R.K., McMeekin, T.A., Stokes, A.N. & Chandler, R.E. 1983. Model for bacterial culture growth rate throughout the entire biokinetic temperature range. Journal of bacteriology, 154, 12221226.
Ross, J.C. & Vincent, W.F. 1998. Temperature dependence of UV radiation effects on Antarctic Cyanobacteria. Journal of Phycology, 34, 118125.
Saadoun, I. & Gharaibeh, R. 2003. The Streptomyces flora of Badia region of Jordan and its potential as a source of antibiotics active against antibiotic-resistant bacteria. Journal of Arid Environments, 53, 365371.
Segawa, T., Takeuchi, N., Rivera, A., Yamada, A., Yoshimura, Y., Barcaza, G., Shinbori, K., Motoyama, H., Kohshima, S. & Ushida, K. 2013. Distribution of antibiotic resistance genes in glacier environments. Environmental Microbiology Reports, 5, 127134.
Seipke, R.F. 2015. Strain-level diversity of secondary metabolism in Streptomyces albus . PloS ONE, 10, 10.1371/journal.pone.0116457.
Shirling, E.B. & Gottlieb, D. 1966. Methods for characterization of Streptomyces species. International Journal of Systematic and Evolutionary Microbiology, 16, 313340.
Smith, R.I.L. 1991. Exotic sporomorpha as indicators of potential immigrant colonists in Antarctica. Grana, 30, 313324.
Srivibool, R. & Sukchotiratana, M. 2006. Bioperspective of actinomycetes isolates from coastal soils: a new source of antimicrobial producers. Songklanakarin Journal of Science and Technology, 28, 493499.
Stackebrandt, E., Rainey, F.A. & Ward-Rainey, N.L. 1997. Proposal for a new hierarchic classification system, Actinobacteria classis nov. International Journal of Systematic Bacteriology, 47, 479491.
Subramani, R. & Aalbersberg, W. 2013. Culturable rare Actinomycetes: diversity, isolation and marine natural product discovery. Applied Microbiology and Biotechnology, 97, 92919321.
Tamura, K. & Nei, M. 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10, 512526.
Thakur, D., Yadav, A., Gogoi, B.K. & Bora, T.C. 2007. Isolation and screening of Streptomyces in soil of protected forest areas from the states of Assam and Tripura, India, for antimicrobial metabolites. Journal De Mycologie Medicale, 17, 242249.
Tomova, I., Stoilova-Disheva, M. & Vasileva-Tonkova, E. 2014a. Characterization of heavy metals resistant heterotrophic bacteria from soils in the Windmill Islands region, Wilkes Land, East Antarctica. Polish Polar Research, 35, 593607.
Tomova, I., Gladka, G., Tashyrev, A. & Vasileva-Tonkova, E. 2014b. Isolation, identification and hydrolytic enzymes production of aerobic heterotrophic bacteria from two Antarctic islands. International Journal of Environmental Science, 4, 614625.
Van den Burg, B. 2003. Extremophiles as a source for novel enzymes. Current Opinion in Microbiology, 6, 213218.
Vincent, W.F., Gibson, J.A.E., Pienitz, R., Villeneuve, V., Broady, P.A., Hamilton, P.B. & Howard-Williams, C. 2000. Ice shelf microbial ecosystems in the high Arctic and implications for life on snowball earth. Naturwissenschaften, 87, 137141.
Wallace, R.J., Vance, P., Weissfeld, A. & Martin, R.R. 1978. Beta-lactamase production and resistance to beta-lactam antibiotics in Nocardia. Antimicrobial Agents and Chemotherapy, 14, 704709.
Wong, C.M.V.L., Tam, H.K., Alias, S.A., González, M., González-Rocha, G. & Domínguez-Yévenes, M. 2011. Pseudomonas and Pedobacter isolates from King George Island inhibited the growth of foodborne pathogens. Polish Polar Research, 32, 314.
Yoshida, A., Seo, Y., Suzuki, S., Nishino, T., Kobayashi, T., Hamada-Sato, N., Kogure, K. & Imada, C. 2008. Actinomycetal community structures in seawater and freshwater examined by DGGE analysis of 16S rRNA gene fragments. Marine Biotechnology, 10, 554563.
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Antarctic Science
  • ISSN: 0954-1020
  • EISSN: 1365-2079
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