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Isolation and characterization of the first microsatellite markers for the southern harvester termite, Microhodotermes viator

Published online by Cambridge University Press:  10 May 2016

N. Muna  and
C. O'Ryan
N. Muna*
Affiliation:
Department of Molecular & Cell Biology, University of Cape Town, Western Cape, South Africa
C. O'Ryan
Affiliation:
Department of Molecular & Cell Biology, University of Cape Town, Western Cape, South Africa
*
*Author for correspondence Phone: +27 (0)21 406 6241 E-mail: natashia.muna@uct.ac.za
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Abstract

The southern harvester termite, Microhodotermes viator, is ecologically important due to its nutrient cycling activities and trophic interactions. Additionally, M. viator appears to have very long-lived colonies, which amplifies their effect on the environment. In order to estimate the longevity of a colony it is necessary to understand colony genetic structure. However, intra- and intercolonial genetic structure and levels of relatedness have not yet been examined in this species, likely due to a lack of microsatellite markers that effectively hybridize in this species. Here we describe the identification and characterization of seven microsatellite loci for M. viator, using an enriched approach and a preliminary test of their suitability for studies of fine-scale population genetic structure. Seven polymorphic loci were identified, none of which deviated from Hardy–Weinberg equilibrium. The loci had an average of 5.8 alleles per locus (range: 2–14) and an overall mean heterozygosity of 0.51 ± 0.3. Across all loci, population level pairwise F ST values showed significant genetic differentiation. The loci described and preliminary genetic data presented here provide an invaluable tool for future studies of population structure and longevity in M. viator colonies.

Keywords

Harvester termitemicrohodotermes viatormicrosatellitegenotypepopulation genetics

Information

Type
Research Papers
Information
Bulletin of Entomological Research , Volume 106 , Issue 4 , August 2016 , pp. 488 - 493
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Copyright
Copyright © Cambridge University Press 2016 

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