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Molecular and morphological characterisation of Pseudococcidae surveyed on crops and ornamental plants in Spain

Published online by Cambridge University Press:  18 October 2011

A. Beltrà ,
A. Soto  and
T. Malausa
A. Beltrà*
Affiliation:
Instituto Agroforestal Mediterráneo, Universitat Politècnica de València, Camí de Vera s/n, 46020 Valencia, Spain
A. Soto
Affiliation:
Instituto Agroforestal Mediterráneo, Universitat Politècnica de València, Camí de Vera s/n, 46020 Valencia, Spain
T. Malausa
Affiliation:
Institut National de la Recherche Agronomique, UMR 1301, Equipe BPI. 400, route des Chappes, BP 167, 06903 Sophia-Antipolis Cedex, France
*
*Author for correspondence Fax: 963879269 E-mail: albeliv@etsia.upv.es
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Abstract

Mealybugs (Hemiptera: Pseudococcidae) are common invasive pests in Europe, causing major problems on crops and ornamental plants. However, very few data are available concerning the mealybug fauna of southern Europe. This lack of data and the difficulty of identifying mealybugs morphologically by traditional techniques currently limit the perspectives for efficient specific pest management. The aim of this study was to provide multi-criterion characterization of mealybugs surveyed in eastern Spain in order to facilitate their routine identification through DNA sequencing or the use of derived species-specific molecular tools. We characterised 33 mealybug populations infesting crops and ornamental plants in eastern Spain, using a combination of molecular and morphological techniques, including the sequencing of the universal barcode DNA region cytochrome c oxidase subunit I (COI). This characterisation has led to the identification of ten species and provides sequence data for three previously unsequenced species, contributing to the phylogenetic knowledge of the family Pseudococcidae. In addition, the intraspecific variations found in the populations of five mealybug species provide insight into their invasion history.

Keywords

DNA barcodingmolecular characterizationmealybugspecies identification
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 102 , Issue 2 , April 2012 , pp. 165 - 172
Copyright
Copyright © Cambridge University Press 2011

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References

Ashfaq, M., Noor, A.R. & Mansoor, S. (2010) DNA-based characterization of an invasive mealybug (Hemiptera: Pseudococcidae) species damaging cotton in Pakistan. Applied Entomology and Zoology 45, 395–404.CrossRefGoogle Scholar
Baumann, L., Thao, M.L., Hess, J.M., Johnson, M.W. & Baumann, P. (2002) The genetic properties of the primary endosymbionts of mealybugs differ from those of other endosymbionts of plant sap-sucking insects. Applied and Environmental Microbiology 68, 3198–3205.CrossRefGoogle ScholarPubMed
Belshaw, R. & Quicke, D.L.J. (1997) A molecular phylogeny of the Aphidiinae (Hymenoptera: Braconidae). Molecular Phylogenetics and Evolution 7, 281–293.CrossRefGoogle ScholarPubMed
Beltrà, A. & Soto, A. (2011) New records of mealybugs (Hemiptera: Pseudococcidae) from Spain. Phytoparasitica 39, 385–387.CrossRefGoogle Scholar
Beltrà, A., Soto, A., Germain, J.F., Matile-Ferrero, D., Mazzeo, G., Pellizzari, G., Russo, A., Franco, J.C. & Williams, D.J. (2010) The Bougainvillea mealybug Phenacoccus peruvianus, a rapid invader from South America to Europe. Entomologia Hellenica 19, 137–143.CrossRefGoogle Scholar
Ben-Dov, Y. (1994) A Systematic Catalogue of the Mealybugs of the World: (Insecta; Homoptera; Coccoidea; Pseudococcidae and Putoidae) with Data on Geographical Distribution, Host Plants, Biology and Economic Importance. Andover, MA, USA, Intercept Ltd.Google Scholar
Cavalieri, V., Mazzeo, G., Garzia, G.T., Buonocore, E. & Russo, A. (2008) Identification of Planococcus ficus and Planococcus citri (Hemiptera: Pseudococcidae) by PCR-RFLP of COI gene. Zootaxa 1816, 65–68.CrossRefGoogle Scholar
Charles, J.G., Froud, K.J. & Henderson, R.C. (2000) Morphological variation and mating compatibility within the mealybugs Pseudococcus calceolariae and P. similans (Hemiptera: Pseudococcidae), and a new synonymy. Systematic Entomology 25, 285–294.CrossRefGoogle Scholar
Cox, J.M. (1983) An experimental study of morphological variation in mealybugs (Homoptera: Coccoidea: Pseudococcidae). Systematic Entomology 8, 361–382.CrossRefGoogle Scholar
Demontis, M.A., Ortu, S., Cocco, A., Lentini, A. & Migheli, Q. (2007) Diagnostic markers for Planococcus ficus (Signoret) and Planococcus citri (Risso) by random amplification of polymorphic DNA-polymerase chain reaction and species-specific mitochondrial DNA primers. Journal of Applied Entomology 131, 59–64.Google Scholar
Dlugosch, K.M. & Parker, I.M. (2008) Founding events in species invasions: genetic variation, adaptive evolution, and the role of multiple introductions. Molecular Ecology 17, 431–449.CrossRefGoogle ScholarPubMed
Downie, D.A. & Gullan, P.J. (2004) Phylogenetic analysis of mealybugs (Hemiptera: Coccoidea: Pseudococcidae) based on DNA sequences from three nuclear genes, and a review of the higher classification. Systematic Entomology 29, 238–259.CrossRefGoogle Scholar
Franco, J.C., Silva, E.B. & Carvalho, J.P. (2000) Mealybugs (Hemiptera, Pseudococcidae) Associated with Citrus in Portugal. Lisbon, Portugal, ISA Press.Google Scholar
Gimpel, W. & Miller, D.R. (1996) Systematic Analysis of the Mealybugs in the Pseudococcus Maritimus Complex (Homoptera: Pseudococcidae). Gainesville, FL, USA, Associated Publishers.Google Scholar
Granara de Willink, M.C. & Szumik, C. (2007) Phenacoccinae de Centro y Sudamérica (Hemiptera: Coccoidea: Pseudococcidae): Sistemática y Filogenia. Revista de la Sociedad Entomológica Argentina 66, 29–129.Google Scholar
Gullan, P.J., Downie, D.A. & Steffan, S.A. (2003) A new pest species of the mealybug genus Ferrisia Fullaway (Hemiptera: Pseudococcidae) from the United States. Annals of the Entomological Society of America 96, 723–737.CrossRefGoogle Scholar
Gullan, P.J., Kaydan, M.B. & Hardy, N.B. (2010) Molecular phylogeny and species recognition in the mealybug genus Ferrisia Fullaway (Hemiptera: Pseudococcidae). Systematic Entomology 35, 329–339.CrossRefGoogle Scholar
Hall, T. (1999) Bioedit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symposium Series 41, 95–98.Google Scholar
Hardy, N.B., Gullan, P.J. & Hodgson, C.J. (2008) A subfamily-level classification of mealybugs (Hemiptera: Pseudococcidae) based on integrated molecular and morphological data. Systematic Entomology 33, 51–71.CrossRefGoogle Scholar
Hebert, P.D.N., Cywinska, A., Ball, S.L. & deWaard, J.R. (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society, Series B: Biological Sciences 270, 313–321.CrossRefGoogle ScholarPubMed
Heinze, K. (1952) Polyvinylalkohol-lactophenol-gemisch als einbettungsmittel fur blattlause. Die Naturwissenschaften 39, 285–286.CrossRefGoogle Scholar
Hulme, P.E., Bacher, S., Kenis, M., Klotz, S., Kühn, I., Minchin, D., Nentwig, W., Olenin, S., Panov, V., Pergl, J., Pyšek, P., Roques, A., Sol, D., Solarz, W. & Vilà, M. (2008) Grasping at the routes of biological invasions: a framework for integrating pathways into policy. The Journal of Applied Ecology 45, 403–414.Google Scholar
Kenis, M., Auger-Rozenberg, M., Roques, A., Timms, L., Péré, C., Cock, M.J.W., Settele, J., Augustin, S. & Lopez-Vaamonde, C. (2009) Ecological effects of invasive alien insects. Biological Invasions 11, 21–45.CrossRefGoogle Scholar
Kosztarab, M. & Kozar, F. (1988) Scale Insects of Central Europe. Dordrecht, Netherlands, Dr W. Junk Publishers.Google Scholar
Malausa, T., Fenis, A., Warot, S., Germain, J.-F., Ris, N., Prado, E., Botton, M., Vanlerberghe-Masutti, F., Sforza, R., Cruaud, C., Couloux, A. & Kreiter, P. (2011) DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae). Journal of Applied Entomology 135, 142–155.CrossRefGoogle Scholar
Marotta, S. & Tranfaglia, A. (1990) New and little known species of Italian scale insects (Homoptera: Coccoidea). pp. 107–112 in Proceedings of the Sixth International Symposium of Scale Insect Studies, Part II. 6–12 August 1990, Crackow, Poland, Agricultural University Press.Google Scholar
Miller, D.R., Miller, G.L. & Watson, G.W. (2002) Invasive species of mealybugs (Hemiptera: Pseudococcidae) and their threat to US agriculture. Proceedings of the Entomological Society of Washington 104, 825–836.Google Scholar
Miller, D.R., Miller, G.L., Hodges, G.S. & Davidson, J.A. (2005) Introduced scale insects (Hemiptera: Coccoidea) of the United States and their impact on US agriculture. Proceedings of the Entomological Society of Washington 107, 123–158.Google Scholar
Pagel, M. & Meade, A. (2004) A phylogenetic mixture model for detecting pattern-heterogeneity in gene sequence or character-state data. Systematic Biology 53, 571–581.Google Scholar
Park, D.S., Suh, S.J., Hebert, P.D.N., Oh, H.W. & Hong, K.J. (2011) DNA barcodes for two scale insects families, mealybugs (Hemiptera: Pseudococcidae) and armored scales (Hemiptera: Diaspididae). Bulletin of Entomological Research 101, 429–434.CrossRefGoogle ScholarPubMed
Pellizzari, G. & Germain, J. (2010) Scales (Hemiptera, Superfamily Coccoidea). BioRisk 4, 475–510.CrossRefGoogle Scholar
Pieterse, W., Muller, D. & van Vuuren, B. (2010) A molecular identification approach for five species of mealybug (Hemiptera: Pseudococcidae) on citrus fruit exported from South Africa. African Entomology 18, 23–28.CrossRefGoogle Scholar
Pimentel, D., McNair, S., Janecka, J., Wightman, J., Simmonds, C., O'Connell, C., Wong, E., Russel, L., Zern, J., Aquino, T. & Tsomondo, T. (2001) Economic and environmental threats of alien plant, animal, and microbe invasions. Agriculture Ecosystems & Environment 84, 1–20.CrossRefGoogle Scholar
Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572–1574.CrossRefGoogle ScholarPubMed
Roques, A., Rabitsch, W., Rasplus, J., Lopez-Vaamonde, C., Nentwig, W. & Kenis, M. (2009) Alien Terrestrial Invertebrates of Europe. pp. 63–79in DAISIE (Ed.) Handbook of Alien Species in Europe. Dordrecht, Netherlands, Springer.CrossRefGoogle Scholar
Rung, A., Scheffer, S.J., Evans, G. & Miller, D. (2008) Molecular identification of two closely related species of mealybugs of the genus Planococcus (Homoptera: Pseudococcidae). Annals of the Entomological Society of America 101, 525–532.CrossRefGoogle Scholar
Rung, A., Miller, D.R. & Scheffer, S.J. (2009) Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Method to Distinguish Three Mealybug Groups Within the Planococcus citri-P. minor Species Complex (Hemiptera: Coccoidea: Pseudococcidae). Journal of Economic Entomology 102, 8–12.Google Scholar
Saccaggi, D.L., Pietersen, G. & Kruger, K. (2008) A multiplex PCR assay for the simultaneous identification of three mealybug species (Hemiptera: Pseudococcidae). Bulletin of Entomological Research 98, 27–33.CrossRefGoogle ScholarPubMed
Swofford, D. (2003) Paup*: phylogenetic analysis using parsimony (* and other methods). Version 4. Sunderland, UK, Sinauer Associates.Google Scholar
Thompson, J.N. (1998) Rapid evolution as an ecological process. Trends in Ecology & Evolution 13, 329–332.CrossRefGoogle ScholarPubMed
Williams, D.J. (1985) Australian Mealybugs. London, UK, British Museum (Natural History).Google Scholar
Williams, D.J. (2004) Mealybugs of Southern Asia. London, UK, The Natural History Museum and Southern SDN.Google Scholar
Williams, D.J. & Granara de Willink, M.C. (1992) Mealybugs of Central and South America. London, UK, CAB International.Google Scholar
Williamson, M. (1996) Biological Invasions. London, UK, Chapman Hall Ltd.Google Scholar
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