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Detection rates of aphid DNA in the guts of larval hoverflies and potential links to the provision of floral resources

Published online by Cambridge University Press:  24 February 2022

Dylan Hodgkiss ,
Mark J. F. Brown ,
Michelle T. Fountain Open the ORCID record for Michelle T. Fountain [Opens in a new window]  and
Elizabeth L. Clare
Dylan Hodgkiss
Affiliation:
Centre for Ecology, Evolution & Behaviour, Department of Biological Sciences, School of Life Sciences and the Environment, Royal Holloway University of London, London TW20 0EX, UK NIAB EMR, New Road, East Malling, Kent ME19 6RN, UK
Mark J. F. Brown
Affiliation:
Centre for Ecology, Evolution & Behaviour, Department of Biological Sciences, School of Life Sciences and the Environment, Royal Holloway University of London, London TW20 0EX, UK
Michelle T. Fountain*
Affiliation:
NIAB EMR, New Road, East Malling, Kent ME19 6RN, UK
Elizabeth L. Clare
Affiliation:
School of Biological and Chemical Sciences, Queen Mary University of London, London E1 4NS, UK Department of Biology, York University, 4700 Keele Street, Toronto, Ontario M3J 1P3, Canada
*
Author for correspondence: Michelle T. Fountain, Email: michelle.fountain@niab.com
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Abstract

Aphidophagous hoverflies (Diptera, Syrphidae, Syrphinae) are common flower visitors and aphid predators in a range of flowering plants, including fruit crops. Here, we investigate whether aphid prey DNA can be detected in the gut contents of hoverfly larvae from a commercial strawberry field as a proof of concept that a molecular approach can be used to measure agricultural biocontrol. We used high-throughput sequencing (HTS) to target insect DNA and compared the resulting data to reference databases containing aphid and hoverfly DNA sequences. We explored what impact incorporating wildflowers within polythene-clad tunnels may have on aphid DNA detection rates in hoverfly larvae. In a randomized block experiment, coriander (Coriandrum sativum), field forget-me-not (Myosotis arvensis) and corn mint (Mentha arvensis) plants were inserted in rows of strawberries. Their effect on aphid DNA detection rates was assessed. Aphid DNA was found in 55 of 149 specimens (37%) validating the method in principle for measuring agricultural services provided by hoverflies. Interestingly, detection rates were higher near plots with forget-me-not than plots with coriander, though detection rates in control plots did not differ significantly from either wildflower species. These findings confirm that hoverflies predate aphids in UK strawberry fields, and that HTS is a viable method of identifying aphid DNA in predatory hoverflies. We comment on the need for further method development to narrow down identifications of both predator and prey. We furthermore provide some evidence that there is an effect of intercropping strawberry crops with wildflowers which may affect aphid consumption in hoverfly larvae.

Keywords

Aphidoideabiocontrolfloral resourcesFragariagut content analysishoverfly
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 112 , Issue 4 , August 2022 , pp. 451 - 457
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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