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Effects of Galanthus nivalis agglutinin (GNA) expressed in tomato leaves on larvae of the tomato moth Lacanobia oleracea (Lepidoptera: Noctuidae) and the effect of GNA on the development of the endoparasitoid Meteorus gyrator (Hymenoptera: Braconidae)

Published online by Cambridge University Press:  09 March 2007

M.E. Wakefield ,
H.A. Bell ,
E.C. Fitches ,
J.P. Edwards  and
A.M.R. Gatehouse
M.E. Wakefield*
Affiliation:
Central Science Laboratory, Sand Hutton, York, YO41 1LZ, UK
H.A. Bell
Affiliation:
Central Science Laboratory, Sand Hutton, York, YO41 1LZ, UK
E.C. Fitches
Affiliation:
Central Science Laboratory, Sand Hutton, York, YO41 1LZ, UK
J.P. Edwards
Affiliation:
Central Science Laboratory, Sand Hutton, York, YO41 1LZ, UK
A.M.R. Gatehouse
Affiliation:
School of Biology, Institute for Research on Environment and Sustainability, Devonshire Building, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK
*
*Fax: +44(0)1904 462111 E-mail: m.wakefield@csl.gov.uk
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Abstract

The effect of ingestion of transgenic tomato leaves expressing the plant lectin Galanthus nivalis agglutinin (GNA) on development of larvae of Lacanobia oleracea (Linnaeus) was studied under laboratory conditions. When L. oleracea larvae were fed on tomato line 14.1H, expressing approximately 2.0% GNA, significant increases in the mean larval weight and in the amount of food consumed were found. This resulted in an overall reduction in the mean development time to the pupal stage of approximately 7 days. A significant increase in the percentage survival to the adult moth was also recorded when newly hatched larvae were reared on transgenic tomato leaves (72%) compared to larvae reared on untransformed leaves (40%). The effects of ingestion of GNA by L. oleracea larvae, via artificial diet or the leaves of transgenic tomato or potato plants, on the subsequent development of its solitary endoparasitoid Meteorus gyrator (Thunberg) was also studied. No significant effects on the life cycle parameters of M. gyrator developing in L. oleracea fed on GNA-containing diets were observed. Experiments with transgenic potato plants indicated that the stadium of the host larvae at parasitism had a greater influence on M. gyrator development than the presence of GNA. Potential GNA-binding glycoproteins were detected in the gut and body tissues of larval M. gyrator. Despite detection in host tissues, GNA could not be detected in adult M. gyrator and therefore it is likely that at the time of pupation M. gyrator are able to void the GNA in the meconial pellet.

Keywords

Lacanobia oleraceaGalanthus nivalis agglutinin (GNA)insect-resistant transgenic plantsMeteorus gyratortritrophic interactions
Type
Review Article
Information
Bulletin of Entomological Research , Volume 96 , Issue 1 , February 2006 , pp. 43 - 52
Copyright
Copyright © Cambridge University Press 2006

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