Hostname: page-component-848d4c4894-p2v8j Total loading time: 0 Render date: 2024-05-03T22:01:57.280Z Has data issue: false hasContentIssue false
  • English
  • Français

Expression profile and function analysis of MsCSP17 and MsCSP18 in the larval development of Mythimna separata

Published online by Cambridge University Press:  07 September 2023

Tingting Chen ,
Xue Zhou ,
Jing Wu ,
Gaoliang Xing ,
Haiqun Cao  and
Yong Huang Open the ORCID record for Yong Huang [Opens in a new window]
Tingting Chen
Affiliation:
Anhui Province Key Laboratory of Crop Integrated Pest Management, College of Plant Protection, Anhui Agricultural University, Hefei, Anhui, China
Xue Zhou
Affiliation:
Anhui Province Key Laboratory of Crop Integrated Pest Management, College of Plant Protection, Anhui Agricultural University, Hefei, Anhui, China
Jing Wu
Affiliation:
Anhui Province Key Laboratory of Crop Integrated Pest Management, College of Plant Protection, Anhui Agricultural University, Hefei, Anhui, China
Gaoliang Xing
Affiliation:
Anhui Province Key Laboratory of Crop Integrated Pest Management, College of Plant Protection, Anhui Agricultural University, Hefei, Anhui, China
Haiqun Cao*
Affiliation:
Anhui Province Key Laboratory of Crop Integrated Pest Management, College of Plant Protection, Anhui Agricultural University, Hefei, Anhui, China
Yong Huang*
Affiliation:
Anhui Province Key Laboratory of Crop Integrated Pest Management, College of Plant Protection, Anhui Agricultural University, Hefei, Anhui, China
*
Corresponding authors: Yong Huang; Email: yongh2017@ahau.edu.cn; Haiqun Cao; Email: caohq@ahau.edu.cn
Corresponding authors: Yong Huang; Email: yongh2017@ahau.edu.cn; Haiqun Cao; Email: caohq@ahau.edu.cn
Get access Rights & Permissions [Opens in a new window]

Abstract

Chemosensory proteins (CSPs) were necessary for insect sensory system to perform important processes such as feeding, mating, spawning, and avoiding natural enemies. However, their functions in non-olfactory organs have been poorly studied. To clarify the function of CSPs in the development of Mythimna separata (Walker) larvae, two CSP genes, MsCSP17 and MsCSP18, were identified from larval integument transcriptome dataset. Both of MsCSP17 and MsCSP18 contained four conserved cysteine sites (C × (6)-C × (18)-C × (2)-C), with a signal peptide at the N-terminal. RT-qPCR analysis showed that MsCSP17 and MsCSP18 have different expression patterns among different developmental stages and tissues. MsCSP17 was highly expressed in 1st–4th instar larvae, and MsCSP18 had high expression in adults. Both genes were expressed highly in larval head, thorax, integument and mandible. Moreover, both of MsCSP17 and MsCSP18 were lowly expressed in larval integuments when larvae molted for 6 h and 9 h from 3rd to 4th instar, but highly at the beginning and end phase during molting. After injection of dsMsCSP17 and dsMsCSP18, the expression levels of two genes decreased significantly, with the body weight of larvae decreased, the mortality increased, and the eclosion rate decreased. It was suggested that MsCSP17 and MsCSP18 contributed to the development of M. separata larvae.

Keywords

Chemosensory proteindevelopmentintegumentMythimna separataRNAi
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 113 , Issue 5 , October 2023 , pp. 676 - 683
Check for updates
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

*

These authors have contributed equally to this work.

References

Almagro Armenteros, JJ, Tsirigos, KD, Sønderby, CK, Petersen, TN, Winther, O, Brunak, S, von Heijne, G and Nielsen, H (2019) SignalP 5.0 improves signal peptide predictions using deep neural networks. Nature Biotechnology 37, 420–423.CrossRefGoogle ScholarPubMed
Antony, B, Soffan, A, Jakše, J, Abdelazim, MM, Aldosari, SA, Aldawood, AS and Pain, A (2016) Identification of the genes involved in odorant reception and detection in the palm weevil Rhynchophorus ferrugineus, an important quarantine pest, by antennal transcriptome analysis. BMC Genomics 17, 69.CrossRefGoogle ScholarPubMed
Cao, DP, Liu, Y, Wei, JJ, Liao, XY, Walker, WB, Li, JH and Wang, GR (2014) Identification of candidate olfactory genes in Chilo suppressalis by antennal transcriptome analysis. International Journal of Biological Sciences 10, 846–860.CrossRefGoogle ScholarPubMed
Cheng, DF, Lu, YY, Zeng, L, Liang, GW and He, XF (2015) Si-CSP9 regulates the integument and moulting process of larvae in the red imported fire ant, Solenopsis invicta. Scientific Reports 5, 9245.CrossRefGoogle ScholarPubMed
Davis, MW and Jorgensen, EM (2022) ApE, A plasmid Editor: a freely available DNA manipulation and visualization program. Frontiers in Bioinformatics 2, 818619.CrossRefGoogle Scholar
Duan, Y, Gong, ZJ, Wu, RH, Miao, J, Jiang, YL, Li, T, Wu, XB and Wu, YQ (2017) Transcriptome analysis of molecular mechanisms responsible for light-stress response in Mythimna separata (Walker). Scientific Reports 7, 45188.CrossRefGoogle ScholarPubMed
Gasteiger, E, Hoogland, C, Gattiker, A, Duvaud, S, Wilkins, MR, Appel, RD and Bairoch, A (2005) Protein identification and analysis tools on the Expasy Server. In Walker, JM (ed.), The Proteomics Protocols Handbook. Clifton, NJ: Humana Press, pp. 571–607.CrossRefGoogle Scholar
Gong, L, Luo, Q, Rizwan-ul-Haq, M and Hu, MY (2012) Cloning and characterization of three chemosensory proteins from Spodoptera exigua and effects of gene silencing on female survival and reproduction. Bulletin of Entomological Research 102, 600–609.CrossRefGoogle ScholarPubMed
Gu, SH, Wang, SY, Zhang, XY, Ji, P, Liu, J, Wang, GR, Wu, KM, Gou, YY, Zhou, JJ and Zhang, YJ (2012) Functional characterizations of chemosensory proteins of the alfalfa plant bug Adelphocoris lineolatus indicate their involvement in host recognition. PLoS One 7, e42871.CrossRefGoogle ScholarPubMed
Guo, W, Wang, XH, Ma, ZY, Xue, L, Han, JY, Yu, D and Kang, L (2011) CSP and takeout genes modulate the switch between attraction and repulsion during behavioral phase change in the migratory locust. PLoS Genetics 7, e1001291.CrossRefGoogle ScholarPubMed
Huang, Y, Liao, M, Yang, QQ, Xiao, JJ, Hu, ZY, Zhou, LJ and Cao, HY (2018) Transcriptome profiling reveals differential gene expression of detoxification enzymes in Sitophilus zeamais responding to terpinen-4-ol fumigation. Pesticide Biochemistry and Physiology 149, 44–53.CrossRefGoogle ScholarPubMed
Huang, Y, Liao, M, Yang, QQ, Shi, S, Xiao, JJ and Cao, HQ (2020) Knockdown of NADPH-cytochrome P450 reductase and CYP6MS1 increases the susceptibility of Sitophilus zeamais to terpinen-4-ol. Pesticide Biochemistry and Physiology 162, 15–22.CrossRefGoogle ScholarPubMed
Huang, Y, Yin, HQ, Zhu, Z, Jiang, XC, Li, XX, Dong, YC, Sheng, CW, Liao, M and Cao, HQ (2021) Expression and functional analysis of cytochrome P450 genes in the integument of the oriental armyworm, Mythimna separata (Walker). Pest Management Science 77, 577–587.CrossRefGoogle ScholarPubMed
Jiang, XF, Luo, LZ, Zhang, L, Sappington, TW and Hu, Y (2011) Regulation of migration in Mythimna separata (Walker) in China: a review integrating environmental, physiological, hormonal, genetic, and molecular factors. Environmental Entomology 40, 516–533.CrossRefGoogle Scholar
Jiang, XF, Zhang, L, Cheng, YX and Luo, LZ (2014) Current status and trends in research on the oriental armyworm, Mythimna separata (Walker) in China. Chinese Journal of Applied Entomology 51, 881–889.Google Scholar
Kaupp, UB (2010) Olfactory signalling in vertebrates and insects: differences and commonalities. Nature Reviews Neuroscience 11, 188–200.CrossRefGoogle ScholarPubMed
Kitabayashi, AN, Arai, T, Kubo, T and Natori, S (1998) Molecular cloning of cDNA for p10, a novel protein that increases in the regenerating legs of Periplaneta americana American cockroach. Insect Biochemistry and Molecular Biology 28, 785–790.CrossRefGoogle ScholarPubMed
Kumar, S, Stecher, G and Tamura, K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33, 1870–1874.CrossRefGoogle ScholarPubMed
Leal, WS (2013) Odorant reception in insects: roles of receptors, binding proteins, and degrading enzymes. Annual Review of Entomology 58, 373–391.CrossRefGoogle ScholarPubMed
Liu, Y, Qi, M, Chi, Y and Wuriyanghan, H (2016) De Novo assembly of the transcriptome for oriental armyworm Mythimna separata (Lepidoptera: Noctuidae) and analysis on insecticide resistance-related genes. Journal of Insect Science 16, 1–8.CrossRefGoogle ScholarPubMed
Liu, Z, Wang, X, Lei, C and Zhu, F (2017) Sensory genes identification with head transcriptome of the migratory armyworm, Mythimna separata. Scientific Reports 7, 46033.CrossRefGoogle ScholarPubMed
Lv, M, Wu, W and Liu, H (2014) Effects of fraxinellone on the midgut enzyme activities of the 5th instar larvae of oriental armyworm, Mythimna separata walker. Toxins 6, 2708–2718.CrossRefGoogle ScholarPubMed
Ma, C, Cui, SW, Tian, ZY, Zhang, Y, Chen, GM, Gao, XY, Tian, ZQ, Chen, HS, Gou, JY and Zhou, ZS (2019) OcomCSP12, a chemosensory protein expressed specifically by ovary, mediates reproduction in Ophraella communa (Coleoptera: Chrysomelidae). Frontiers in Physiology 10, 1290.CrossRefGoogle ScholarPubMed
Pelosi, P, Iovinella, I, Felicioli, A and Dani, FR (2014) Soluble proteins of chemical communication: an overvie. Frontiers in Physiology 5, 320.CrossRefGoogle Scholar
Pelosi, P, Iovinella, I, Zhu, J, Wang, GR and Dani, FR (2018) Beyond chemoreception: diverse tasks of soluble olfactory proteins in insects. Biological Reviews of the Cambridge Philosophical Society 93, 184–200.CrossRefGoogle ScholarPubMed
Untergasser, A, Cutcutache, I, Koressaar, T, Ye, J, Faircloth, BC, Remm, M and Rozen, SG (2012) Primer3-new capabilities and interfaces. Nucleic Acids Research 40, e115.CrossRefGoogle ScholarPubMed
Wang, ZF, Yang, PC, Chen, DF, Jiang, F, Li, Y, Wang, XH and Kang, L (2015) Identification and functional analysis of olfactory receptor family reveal unusual characteristics of the olfactory system in the migratory locust. Cellular and Molecular Life Sciences 72, 4429–4443.CrossRefGoogle ScholarPubMed
Wang, R, Zhang, XM, Li, HL, Guo, XJ and Luo, C (2016) Identification and expression profiling of five chemosensory protein genes in the whitefly MED, Bemisia tabaci. Journal of Asia-Pacific Entomology 19, 195–201.CrossRefGoogle Scholar
Wanner, KW, Willis, LG, Theilmann, DA, Isman, MB, Feng, Q and Plettner, E (2004) Analysis of the OS-D-like gene family. Journal of Chemical Ecology 30, 889–911.CrossRefGoogle ScholarPubMed
Waterhouse, A, Bertoni, M, Bienert, S, Studer, G, Tauriello, G, Gumienny, R, Heer, FT, de Beer, TAP, Rempfer, C, Bordoli, L, Lepore, R and Schwede, T (2018) SWISS-MODEL: homology modelling of protein structures and complexes. Nucleic Acids Research 46, W296–W303.CrossRefGoogle ScholarPubMed
Xue, WX, Fan, J, Zhang, Y, Xu, QX, Han, ZL, Sun, JR and Chen, JL (2016) Identification and expression analysis of candidate odorant-binding protein and chemosensory protein genes by antennal transcriptome of Sitobion avenae. PLoS One 11, e0161839.CrossRefGoogle ScholarPubMed
Younas, A, Khan, Z, Wakil, W, Shaaban, M and Prager, SM (2016) The efficacy of Beauveria bassiana, jasmonic acid and chlorantraniliprole on larval populations of Helicoverpa armigera in chickpea crop ecosystems. Pest Management Science 73, 418–424.CrossRefGoogle ScholarPubMed
Zhang, ZK and Lei, ZR (2015) Identification, expression profiling and fluorescence-based binding assays of a chemosensory protein gene from the Western flower thrips, Frankliniella occidentalis. PLoS One 10, e0117726.CrossRefGoogle ScholarPubMed
Zhang, YN, Jin, JY, Jin, R, Xia, YH, Zhou, JJ, Deng, JY and Dong, SL (2013) Differential expression patterns in chemosensory and non-chemosensory tissues of putative chemosensory genes identified by transcriptome analysis of insect pest the purple stem borer Sesamia inferens (Walker). PLoS One 8, e69715.CrossRefGoogle ScholarPubMed
Zhao, HL, Zhu, Z, Xing, GL, Li, YY, Zhou, X, Wang, JJ, Li, GT, Cao, HQ and Huang, Y (2022) Deformed mediated larval incisor lobe development causes differing feeding behavior between oriental armyworm and fall armyworm. Insects 13, 594.CrossRefGoogle ScholarPubMed
Supplementary material: File

Chen et al. supplementary material
Download undefined(File)
File 12.5 KB