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Genetic variability in the natural populations of Lasioderma serricorne (F.) (Coleoptera: Anobiidae), detected by RAPD markers and by esterase isozymes

Published online by Cambridge University Press:  13 October 2015

T. Coelho-Bortolo ,
C.A. Mangolin  and
A.S. Lapenta
T. Coelho-Bortolo
Affiliation:
Department of Cell Biology and Genetics, State University of Maringá, 87020-900 Maringá, Paraná, Brazil
C.A. Mangolin
Affiliation:
Department of Cell Biology and Genetics, State University of Maringá, 87020-900 Maringá, Paraná, Brazil
A.S. Lapenta*
Affiliation:
Department of Cell Biology and Genetics, State University of Maringá, 87020-900 Maringá, Paraná, Brazil
*
*Author for correspondence Phone: 55 44 30114342 E-mail: aslapenta@uem.br
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Abstract

Lasioderma serricorne (F.) is a small cosmopolitan beetle regarded as a destructive pest of several stored products such as grains, flour, spices, dried fruit and tobacco. Chemical insecticides are one of the measures used against the pest. However, intensive insecticide use has resulted in the appearance of resistant insect populations. Therefore, for the elaboration of more effective control programs, it is necessary to know the biological aspects of L. serricorne. Among these aspects, the genetic variability knowledge is very important and may help in the development of new control methods. The objective of this study was to evaluate the genetic variability of 11 natural populations of L. serricorne collected respectively in three and four towns in the states of Paraná and São Paulo, Brazil, using 20 primers random amplified polymorphic DNA (RAPD) and polymorphisms of esterases. These primers produced 352 polymorphic bands. Electrophoretic analysis of esterases allowed the identification of four polymorphic loci (Est-2, Est-4, Est-5 and Est-6) and 18 alleles. Results show that populations are genetically differentiated and there is a high level of genetic variability within populations. The high degree of genetic differentiation is not directly correlated to geographical distance. Thus, our data indicate that movement of infested commodities may contribute to the dissemination of L. serricorne, facilitating gene flow.

Keywords

Lasioderma serricornegenetic structurepolymorphismgenetic variability
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 106 , Issue 1 , February 2016 , pp. 47 - 53
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Copyright
Copyright © Cambridge University Press 2015 

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