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Habitat visualization, acquisition features and necessity of the gammaproteobacterial symbiont of pistachio stink Bug, Acrosternum heegeri (Hem.: Pentatomidae)

Published online by Cambridge University Press:  13 June 2019

M. Kashkouli ,
Y. Fathipour Open the ORCID record for Y. Fathipour [Opens in a new window]  and
M. Mehrabadi
M. Kashkouli
Affiliation:
Department of Entomology, Faculty of Agriculture, Tarbiat Modares University, P.O.Box 14115-336, Tehran, Iran
Y. Fathipour*
Affiliation:
Department of Entomology, Faculty of Agriculture, Tarbiat Modares University, P.O.Box 14115-336, Tehran, Iran
M. Mehrabadi
Affiliation:
Department of Entomology, Faculty of Agriculture, Tarbiat Modares University, P.O.Box 14115-336, Tehran, Iran
*
*Author for correspondence Phone: +98 21 48292301 Fax: +98 21 48292200 E-mail: fathi@modares.ac.ir
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Abstract

Plant-sucking stinkbugs are especially associated with mutualistic gut bacterial symbionts. Here, we explored the symbiotic relationship of a pistachio stinkbug, Acrosternum heegeri Fieber by histological, fluorescence in situ hybridization (FISH), real-time PCR and molecular phylogenetic techniques. Furthermore, the effects of the symbiont on the resting/wandering behaviors of the newborn nymphs, pre-adult survival rates, and stage compositions were investigated. Transmission electron microscopy and real-time PCR analyses showed that a rod-shaped gammaproteobacterium was persistently located within the posterior midgut crypts. Molecular phylogenetic and FISH techniques strongly suggested that this symbiont should be placed in the genus Pantoea of the Enterobacteriales. Scanning electron microscopy confirmed the presence of the bacterial cells on the egg surface which the surface sterilization of the eggs resulted in the successful removal of the symbiont from the eggs. Symbiotic and aposymbiotic A. heegeri showed no significant differences in the wandering behaviors of the first nymphal stages, while the symbiont-free insects suffered retarded growth and lower survivability. Together, the results highlight the habitat and acquisition features of Pantoea symbiont and its contribution in A. heegeri biology that might help us for better pest management in the future.

Keywords

Heritable symbiontpentatomidaegammaproteobacteriasymbiont elimination
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 110 , Issue 1 , February 2020 , pp. 22 - 33
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Copyright
Copyright © Cambridge University Press 2019 

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