Skip to main content
×
Home

Larval species composition and genetic structures of Carposina sasakii, Grapholita dimorpha, and Grapholita molesta from Korea

  • D.H. Kwon (a1), H.K. Kwon (a2), D.H. Kim (a2) and C.Y. Yang (a2)
Abstract
Abstract

Rapid determination of the larval species composition and understanding of their genetic structure is important to establish the appropriate management system for multiple species infesting in fruits. We established accurate and rapid diagnostic methods based on multiplex polymerase chain reaction (PCR) diagnostic techniques to discriminate the three major lepidopteran species in orchard, Carposina sasakii, Grapholita dimorpha, and Grapholita molesta. Each species was identified by amplifying species-specific PCR products (375 bp for C. sasakii, 125 and 234 bp for G. dimorpha, and 125 bp for G. molesta). Based on species composition analysis from six types of infested fruits, G. dimorpha constituted the highest proportion (47.8%), followed by 35.2 and 13.5% for G. molesta and C. sasakii, respectively. Interestingly, high prevalence was found in G. dimorpha and G. molesta for plum and peach, respectively. Based on genetic diversity analysis, the three insect species exhibited moderate or high haplotype diversity and low nucleotide diversity, ranging from 0.319 to 0.699 and 0.0006 to 0.0045, respectively. Demographic expansion was not detected according to either a neutrality test or mismatch distribution analysis. Moreover, no significant genetic structure corresponding to province, host plant, fruit type, or collection period was observed. These results suggest that the population of each species would have high dispersal ability following fruit-generating periods via intrinsic host adaptation ability regardless of the spatial and temporal conditions. Determination of larval composition on fruit is valuable for establishing appropriate management systems that take the species into consideration; additionally, population genetic approaches can be utilized to understand the effects of environmental factors (province, host fruit, fruit type, etc.) on population structures.

Copyright
Corresponding author
*Author for correspondence Phone: +01182-2-880-4827 (D.H. Kwon), +01182-63-238-6331 (C.Y. Yang) Fax: +01182-2-873-2319 (D.H. Kwon), +01182-63-238-6305 (C.Y. Yang) E-mail: jota486486@snu.ac.kr (D.H. Kwon) and cyyang@korea.kr (C.Y. Yang)
References
Hide All
Ahn S.-J., Choi K.-H., Kang T.J., Kim H.H., Kim D.-H., Cho M.R. & Yang C.Y. (2013) Molecular diagnosis of Grapholita molesta and Grapholita dimorpha and their different occurrence in peach and plum. Korean Journal of Applied Entomology 52, 365370.
Barcenas N.M., Unruh T.R. & Neven L.G. (2005) DNA diagnostics to identify internal feeders (Lepidoptera: Tortricidae) of pome fruits of quarantine importance. Journal of Economic Entomology 98, 299306.
Beljaev E. & Ponomarenko M. (2005) New lepidopterological finds (Lepidoptera: Gelechiidae, Tortricidae, Geometridae) in south of Russian Far East. Far Eastern Entomologist 155, 111.
Chaudhry G.-U. (1956) The development and fecundity of the oriental fruit moth, Grapholitha (Cydia) molesta (Busck) under controlled temperatures and humidities. Bulletin of Entomological Research 46, 869898.
Chen M. & Dorn S. (2009) Reliable and efficient discrimination of four internal fruit-feeding Cydia and Grapholita species (Lepidoptera: Tortricidae) by polymerase chain reaction-restriction fragment length polymorphism. Journal of Economic Entomology 102, 22092216.
Choi K.-H., Lee S.-W., Lee D.-H., Kim D.-A. & Kim S.-K. (2008) Recent occurrence status of two major fruit moths, oriental fruit moth and peach fruit moth in apple orchards. Korean Journal of Applied Entomology 47, 1722.
Choi K.-H., Lee D.-H., Byun B.-K. & Mochizuki F. (2009) Occurrence of Grapholita dimorpha Komai (Lepidoptera: Tortricidae), a new insect pest in apple orchards of Korea. Korean Journal of Applied Entomology 48, 417421.
Choi K.S., Lee S.-Y., Choi K.-H., Jang I., Kang I.-K. & Jung H.-Y. (2016) Simultaneous identification of Grapholita molesta Busck and Grapholita dimorpha Komai by PCR-RFLP. Entomological Research 46, 206209.
Edgar R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32, 17921797.
Excoffier L. & Lischer H.E. (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources 10, 564567.
Folmer O., Hoeh W., Black M. & Vrijenhoek R. (1994) Conserved primers for PCR amplification of mitochondrial DNA from different invertebrate phyla. Molecular Marine Biology and Biotechnology 3, 294299.
Hada H. & Sekine K.-T. (2011) A diagnostic multiplex polymerase chain reaction method to identify Japanese internal apple-feeding Lepidopteran pests: Grapholita molesta, Grapholita dimorpha (Lepidoptera: Tortricidae), and Carposina sasakii (Lepidoptera: Carposinidae). Applied Entomology and Zoology 46, 287291.
Ishiguri Y. & Shirai Y. (2004) Flight activity of the peach fruit moth, Carposina sasakii (Lepidoptera: Carposinidae), measured by a flight mill. Applied Entomology and Zoology 39, 127131.
Jeong S.A., Sah L.P., Ahn J.J., Kim Y.I. & Jung C. (2012) Occurrence patterns of three major fruit moths, Grapholita molesta, Grapholita dimorpha and Carposina sasakii, monitored by sex pheromone in plum orchards. Korean Journal of Applied Entomology 51, 449459.
Jung C.R. & Kim Y. (2013) Different types of fruit damages of three internal apple feeders diagnosed with mitochondrial molecular markers. Journal of Asia-Pacific Entomology 16, 189197.
Kim D.-S., Lee J.-H. & Yiem M.-S. (2000) Spring emergence pattern of Carposina sasakii (Lepidoptera: Carposinidae) in apple orchards in Korea and its forecasting models based on degree-days. Environmental Entomology 29, 11881198.
Kim D.-S., Lee J.-H. & Yiem M.-S. (2001) Temperature-dependent development of Carposina sasakii (Lepidoptera: Carposinidae) and its stage emergence models. Environmental Entomology 30, 298305.
Kim Y., Bae S., Son Y. & Park J. (2009) Analysis of migration of the oriental fruit moth, Grapholita molesta, in apple-cultivating areas based on population monitoring using sex pheromone and RAPD molecular marker. Korean Journal of Applied Entomology 48, 211219.
Kirk H., Dorn S. & Mazzi D. (2013) Worldwide population genetic structure of the oriental fruit moth (Grapholita molesta), a globally invasive pest. BMC Ecology 13, 12.
Komai F. (1979) A new species of the genus Grapholita Treitschke from Japan allied to the oriental fruit moth, Grapholita molesta (Busck) (Lepidoptera: Tortricidae). Applied Entomology and Zoology 14, 133136.
Komai F. (1999) A taxonomic review of the genus Grapholita and allied genera (Lepidoptera: Tortricidae) in the Palaearctic region. Scandinavian Entomology 55, 1226.
Kwon D.H., Kim D.H., Kim H.H., Lee S.H. & Yang C.Y. (2017) Genetic diversity and structure in apple-infesting pests of Carposina sasakii, Grapholita dimorpha and Grapholita molesta in Korea. Journal of Asia-Pacific Entomology 20, 1316.
Lee S.-Y., Choi K.-S., Choi K.-H., Yoon T.-M. & Jung H.-Y. (2013) Morphological differences between larvae of the oriental fruit moth (Grapholita molesta Busck) and the peach fruit moth (Carposina sasakii Matsumura) in Korea. Applied Microscopy 43, 2126.
Lee S.Y., Choi K.S., Back C.G., Choi K.H., Kang I.K., Jung H.Y. & Ohga S. (2015) Distinction of Grapholita molesta Busck and Grapholita dimorpha Komai larvae based on morphological feature of anal prolegs. Faculty of Agriculture Kyushu University 60, 291295.
Librado P. & Rozas J. (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25, 14511452.
Liu Y., Cheng J. & Mou J. (1997) Review of the advances of the peach fruit-borer (Carposina sasakii Matsumura). Journal of Shandong Agricultural University 28, 207214.
Ma T., Li Y., Sun Z. & Wen X. (2014) (Z,E)-9,12-Tetradecadien-1-Ol: a major sex pheromone component of Euzophera pyriella (Lepidoptera: Pyralididae) in Xinjiang, China. Florida Entomologist 97, 496503.
Machida T. & Aoyama A. (1931) Pests in Korea: Pests of Fruit Trees. Tokyo, Ext Lite Hal, pp. 5357.
Makaji T. (1987) Diagnosis of Fruit Tree Diseases and Pests with Color Plates. Tokyo, Agriculture Publication Incorporation.
Oku T., Ohira Y. & Wakou M. (1988) Preliminary notes on a plum [Prunus salicina] fruit moth, Grapholita dimorpha Komai (Lepidoptera: Tortricidae). Bulletin of the Fruit Trees Research Station Series C, Morioka 15, 4964.
Park K.-T. & Kim J.-M. (1986) Moths collected in the northen part of civilian control line neighbouring DMZ. Korean Journal of Applied Entomology 25, 7783.
Phillips J. & Proctor J.R. (1970) Development of methods for sampling the oriental fruit moth, Grapholita molesta (Lepidoptera: Tortricidae), in an Ontario peach orchard. Canadian Entomologist 102, 454471.
Pree D., Trimble R., Whitty K. & Vickers P. (1994) Control of oriental fruit moth by mating disruption using sex pheromone in the Niagara Peninsula, Ontario. Canadian Entomologist 126, 12871299.
Song M.J., Zhou L.N., Zai Y.D., Zhang X., Xing H.T., Li S.F. & Xiao F. (1994) A preliminary study of Euzophera pyriella . Plant Protection 20, 1315.
Song S.-B., Choi K.-H., Lee S.-W. & Kim Y.-G. (2007) DNA markers applicable for identification of two internal apple feeders, Grapholita molesta and Carposina sasakii . Korean Journal of Applied Entomology 46, 175182.
Sun J.-T., Wang M.-M., Zhang Y.-K., Chapuis M.-P., Jiang X.-Y., Hu G., Yang X.-M., Ge C., Xue X.-F. & Hong X.-Y. (2015) Evidence for high dispersal ability and mito-nuclear discordance in the small brown planthopper, Laodelphax striatellus . Scientific Reports 5, 8045.
Tamura K., Stecher G., Peterson D., Filipski A. & Kumar S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30, 27252729.
Tikhonov N.P. (1962) Orchard fruit moths of the Far east. Zashchita Rastenii 7, 4546.
Wang J., Yu Y., Li L.-L., Guo D., Tao Y.-L. & Chu D. (2015) Carposina sasakii (Lepidoptera: Carposinidae) in its native range consists of two sympatric cryptic lineages as revealed by mitochondrial COI gene sequences. Journal of Insect Science 15, 85.
Wei S.J., Cao L.J., Gong Y.J., Shi B.C., Wang S., Zhang F., Guo X.J., Wang Y.M. & Chen X.X. (2015) Population genetic structure and approximate Bayesian computation analyses reveal the southern origin and northward dispersal of the oriental fruit moth Grapholita molesta (Lepidoptera: Tortricidae) in its native range. Molecular Ecology 24, 40944111.
Yan S., Liu Y. & Li M. (1998) Grapholita dimorpha-a new record pest damaging fruit trees of China. Forest Pest and Disease 18, 1516.
Yang C., Han K. & Boo K. (2001) Occurrence of and damage by the oriental fruit moth, Grapholita molesta (Busck) (Lepidoptera: Tortricidae) in pear orchards. Korean Journal of Applied Entomology 40, 117124.
Yang C.Y., Han K.S., Jung J.K., Boo K.S. & Yiem M.S. (2003) Control of the oriental fruit moth, Grapholita molesta (Busck) (Lepidoptera: Tortricidae) by mating disruption with sex pheromone in pear orchards. Journal of Asia-Pacific Entomology 6, 97104.
Zheng Y., Peng X., Liu G., Pan H., Dorn S. & Chen M. (2013) High genetic diversity and structured populations of the oriental fruit moth in its range of origin. PLoS ONE 8, e78476.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Bulletin of Entomological Research
  • ISSN: 0007-4853
  • EISSN: 1475-2670
  • URL: /core/journals/bulletin-of-entomological-research
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Type Description Title
PDF
Supplementary Materials

Kwon supplementary material
Table S3

 PDF (82 KB)
82 KB
PDF
Supplementary Materials

Kwon supplementary material
Table S4

 PDF (79 KB)
79 KB
PDF
Supplementary Materials

Kwon supplementary material
Table S2

 PDF (76 KB)
76 KB
PDF
Supplementary Materials

Kwon supplementary material
Table S1

 PDF (83 KB)
83 KB
UNKNOWN
Supplementary Materials

Kwon supplementary material
Figure S1

 Unknown (182 KB)
182 KB
UNKNOWN
Supplementary Materials

Kwon supplementary material
Figure S2

 Unknown (353 KB)
353 KB

Metrics

Full text views

Total number of HTML views: 3
Total number of PDF views: 21 *
Loading metrics...

Abstract views

Total abstract views: 89 *
Loading metrics...

* Views captured on Cambridge Core between 31st July 2017 - 22nd November 2017. This data will be updated every 24 hours.