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Mechanisms of insecticide resistance in a temephos selected Culex quinquefasciatus (Diptera: Culicidae) strain from Sri Lanka

Published online by Cambridge University Press:  10 July 2009

H.T.R. Peiris  and
J. Hemingway
H.T.R. Peiris
Affiliation:
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, UK
J. Hemingway
Affiliation:
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, UK
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Abstract

Culex quinquefasciatus Say from Peliyagoda, Sri Lanka, has larval resistance to temephos, malathion, fenitrothion and chlorpyrifos. Biochemical assays on individual resistant and susceptible mosquitoes of this strain showed that there was a good correlation between this resistance and increased esterase activity with both 1-and 2-naphthyl acetate, which appears to be the major resistance mechanism in this multiple organophosphate resistant strain. There was no significant difference in malaoxon, bendiocarb or propoxur sensitivity of the acetylcholinesterase from the resistant and susceptible strains, indicating that the sensitivity of the target site has not been altered. Biochemical assays on mass homogenates of the resistant and susceptible strains showed no correlation between resistance and the level of glutathione s-transferase activity, or the amount of cytochrome P450 present.

Type
Research Paper
Information
Bulletin of Entomological Research , Volume 80 , Issue 4 , December 1990 , pp. 453 - 457
Copyright
Copyright © Cambridge University Press 1990

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References

Amerasinghe, F.P. (1986) Current studies in vector populations in areas under development in Mahaweli System C. in Proceedings of the workshop on irrigation and vector borne disease transmission. Sri Lanka.Google Scholar
Amin, A.M. & Peiris, H.T.R. (1990) Detection and selection of organophosphate and carbamate insecticide resistance in Culex quinquefasciatus from Saudi Arabia. Medical and Veterinary Entomology 4, 269–273.CrossRefGoogle ScholarPubMed
Bonning, B.C., Hemingway, J., Romi, R. & Majori, G. (in press) Interaction of insecticide resistance genes in field populations of Culex pipiens from Italy in response to changing insecticide selection pressure. Bulletin of Entomological Research 81.Google Scholar
Curtis, C.F. & Pasteur, N. (1981) Organophosphate resistance in vector populations of the complex of Culex pipiens L. (Diptera: Culicidae). Bulletin of Entomological Research 71, 153–161.CrossRefGoogle Scholar
ffrench-Constant, R.H. & Bonning, B.C. (1989) Rapid microtitre plate test distinguishes insecticide resistant acetylcholinesterase genotypes in the mosquitoes Anopheles albimanus, An. nigerrimus and Culex pipiens. Medical and Veterinary Entomology 3, 9–16.CrossRefGoogle ScholarPubMed
Georghiou, G.P. & Pasteur, N. (1978) Electrophoretic esterase patterns in insecticide resistant and susceptible mosquitoes. Journal of Economic Entomology 71, 201–205.Google Scholar
Georghiou, G.P. & Pasteur, N. (1980) Organophosphate resistance and esterase patterns in natural populations of the southern house mosquitoes from California. Journal of Economic Entomology 73, 489–492.CrossRefGoogle Scholar
Hemingway, J., Smith, C., Jayawardena, K.G.I. & Herath, P.R.J. (1986) Field and laboratory detection of altered acetylcholinesterase resistance genes which confer organophosphate and carbamate resistance in mosquitoes. Bulletin of Entomological Research 76, 559–564.CrossRefGoogle Scholar
Hemingway, J., Callaghan, A. & Amin, A.M. (1990) Mechanisms of organophosphate and carbamate resistance in Culex quinquefasciatus from Saudi Arbaia. Medical and Veterinary Entomology 4, 275–282.CrossRefGoogle Scholar
Hemingway, J., Jayawardena, K.G.I., Weerasinghe, I.S. & Herath, P.R.J. (1987) The use of biochemical tests to identify multiple insecticide resistance mechanisms in field selected populations of Anopheles subpictus (Diptera: Culicidae). Bulletin of Entomological Research 77, 57–66.CrossRefGoogle Scholar
Jayasekera, N., Chellia, R.V., Jansen, C.G. & Pathmanathan, S. (1986) Mosquito studies in Sri Jayawardenapura – the new capital of Sri Lanka. Mosquito Borne Disease Bulletin 2, 87–92.Google Scholar
Magnin, M., Marboutin, E. & Pasteur, N. (1988) Insecticide resistance in Culex auinquefasciatus (Diptera: Culicidae) in West Africa. Journal of Medical Entomology 25, 99–194.Google Scholar
Motoyama, N. & Dauterman, W.C. (1974) The role of non-oxidative metabolism in organophosphate resistance. Journal of Agricultural Food Chemistry 22, 350–356.CrossRefGoogle Scholar
Pasteur, N. & Georghiou, G.P. (1980) Analysis of esterase as a means of determining organophosphate resistance in field populations of Culex pipiens mosquitoes. in Proceedings and papers of the 44th annual conference of the California mosquito and vector control association. Jan. 20–23rd.Google Scholar
Paseur, N., Iseki, A. & Georghiou, G.P. (1981) Genetic and biochemical studies of the highly active esterases A and B associated with organophosphate resistance in mosquitoes of the Culex pipiens complex. Biochemical Genetics 19, 909–919.CrossRefGoogle Scholar
Peiris, H.T.R. (1989) Biochemistry and genetics of organophosphorus and carbamate insecticide resistance in Sri Lankan Culex species. 176 pp. PhD Thesis, University of London.Google Scholar
Peiris, H.T.R. & Hemingway, J. (1990) Temephos resistance and the associated cross-resistance spectrum in a strain of Culex quinquefasciatus from Peliyagoda, Sri Lanka. Bulletin of Entomological Research 80, 49–55.Google Scholar
Ranasinghe, L.E. & Georghiou, G.P. (1979) Comparative modification of insecticide resistance spectrum of Culex pipiens fatigans Wied. by selection with temephos and temephos/synergist combinations. Pesticide Science 10, 502–508.Google Scholar
Raymond, M., Fournier, D., Bride, J.M., Cuany, A., Berge, J., Magnin, M. & Pasteur, N. (1986) Identification of resistance mechanism in Culex pipiens (Diptera: Culicidae) from southern France: Insensitive acetylcholinesterase and detoxifying oxidases. Journal of Economic Entomology 79, 1452–1458.Google Scholar
Stone, B.F. & Brown, A.W.A. (1969) Mechanisms of resistance to fenthion in Culex pipiens fatigans Wied. Bulletin of the World Health Organization 40, 401–408.Google ScholarPubMed
Takahashi, M. & Yasutomi, K. (1987) Insecticide resistance of Culex tritaeniorhynchus (Diptera: Culicidae) in Japan: Genetics and mechanisms of resistance to organophosphorus insecticides. Journal of Medical Entomology 24, 595–603.CrossRefGoogle ScholarPubMed
Villani, F. & Hemingway, J. (1987) The detection and interaction of multiple organophosphorus and carbamate insecticide resistance genes in field populations of Culex pipiens from Italy. Pesticide Biochemistry and Physiology 27, 218–228.Google Scholar
Villani, F., White, G.B., Curtis, C.F. & Miles, S.J. (1983) Inheritance and activity of some esterases associated with organophosphate resistance in mosquitoes of the complex Culex pipiens L. (Diptera: Culicidae). Bulletin of Entomological Research 73, 153–170.CrossRefGoogle Scholar
WHO (World Health Organization) (1981) Instructions for determining the susceptibility or resistance of mosquito larvae to insecticides. WHO/VBC/81.807.Google Scholar