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Molecular characterization of whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) populations infesting cassava

  • I. Abdullahi (a1) (a2), S. Winter (a2), G.I. Atiri (a1) and G. Thottappilly (a3)

Bemisia tabaci (Gennadius) populations, collected from cassava and other plants in major cassava-cultivation areas of Sub-saharan Africa and from elsewhere around the world, were studied to determine their biotype status and genetic variation. Random amplified polymorphic DNA–polymerase chain reaction (RAPD–PCR) markers were used to examine the genetic structure of the populations. The dendogram obtained using the neighbour joining method (NJ) split the cassava-associated populations from the non-cassava types with a 100% bootstrap probability. Analysis of molecular variance (AMOVA) of the RAPD fragments revealed that 63.2% of the total variation was attributable to differences among populations, while the differences among groups (host) and within populations accounted for 27.1 and 9.8% respectively. Analysis of the internally transcribed spacer region I (ITS 1) of the ribosomal DNA confirmed that the cassava populations of B. tabaci populations were distinct from non-cassava populations. Experiments to establish whitefly populations on various host plants revealed that cassava-associated populations were restricted to cassava only, whereas B. tabaci from other hosts were polyphagous but did not colonize cassava. Hence, populations of B. tabaci from cassava in Africa represent a distinct group.

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*Fax: +49 (0)531 299 3014 E-mail: S.Winter@BBA.DE
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G.K. Banks , I.D. Bedford , F.J. Beitia , E. Rodríguez-Cerezo & P.G. Markham (1999) A novel geminivirus of Ipomoea indica (Convolvulaceae) from southern Spain. In Plant Disease 83, 486.

T.S. Bellows , T.M. Perring , R.J. Gill & D.H. Headrick (1994) Description of a species of Bemisia (Homoptera, Aleyrodidae). Annals of the Entomological Society of America 87, 195206.

N. Bharathan , W.R. Graves , K.R. Narayanan , D.J. Schuster , H.H. Bryan & R.T. McMillan (1990) Association of double stranded RNA with whitefly-mediated silvering in squash. Plant Pathology 39, 530538.

J. Bird & K. Maramorosch (1978) Viruses and virus diseases associated with whiteflies. Advances in Virus Research 22, 55110.

J.K. Brown , D.R. Frohlich & R.C. Rosell (1995) The sweetpotato or silverleaf whiteflies: biotypes of Bemisia tabaci or a species complex?. Annual Review of Entomology 40, 511534.

J.K. Brown , T.M. Perring , A.D. Cooper , I.D. Bedford & P.G. Markham (2000) Genetic analysis of Bemisia (Hemiptera: Aleyrodidae) populations by isoelectric focusing electrophoresis. Biochemical Genetics 38, 1325.

C. Burban , D.C. Fishpool , C. Fauquet , D. Fargette & J.C. Thouvenel (1992) Host-associated biotypes within West African populations of the whitefly Bemisia tabaci. Journal of Applied Entomology 113, 416423.

B.C. Campbell (1993) Congruent evolution between whiteflies (Homoptera, Aleyrodidae) and their bacterial endosymbionts based on respective 18S and 16S rDNAs. Current Microbiology 26, 129132.

M.A. Clark , L. Baumann , M.A. Munson , P. Baumann , B.C. Campbell , J.E. Duffus , L.S. Osborne & N.A. Moran (1992) The eubacteria endosymbionts of whiteflies constitute a lineage distinct from the endosymbionts of aphids and mealybugs. Current Microbiology 25, 119123.

S. Cohen , J.E. Duffus & H.Y. Liu (1992) A new Bemisia tabaci biotype in the south western United States and its role in silverleaf of squash and transmission of lettuce infectious yellows virus. Phytopathology 82, 8690.

A.S. Costa (1976) Whitefly transmitted plant diseases. Annual Review of Phytopathology 14, 429449.

H.S. Costa & J.K. Brown (1991) Variation in biological characteristics and esterase patterns among populations of Bemisia tabaci, and the association of one population with silverleaf symptom induction. Entomologia Experimentalis et Applicata 61, 211219.

H.S. Costa , J.K. Brown & D.N. Byrne (1991) Host plant selection by the whitefly Bemisia tabaci (Gennadius) (Homoptera, Aleyrodidae) under glasshouse conditions. Journal of Applied Entomology 112, 146152.

H.S. Costa , M.W. Johnson , D.E. Ullman , A.D. Omer & B.E. Tabashnik (1993) Sweetpotato whitefly: analysis of biotypes and distribution in Hawaii. Environmental Entomology 22, 1620.

P.J. De Barro & F. Driver (1997) Use of RAPD–PCR to distinguish the B biotype from other biotypes of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). Australian Journal of Entomology 36, 149152.

P.J. De Barro , F. Driver , J.W.H. Trueman & J. Curran (2000) Phylogenetic relationships of world populations of Bemisia tabaci (Gennadius) using ribosomal ITS 1. Molecular Phylogenetics and Evolution 16, 2936.

V. Dittrich , S.O. Hassan & G.H. Ernst (1985) Sudanese cotton and the whitefly: a case study of the emergence of a new primary pest. Crop Protection 4, 161176.

J. Dubern (1979) Quelques propriétés de la Mosaique Africaine du Manioc. I: La transmission. Phytopathologische Zeitschrift 96, 2539.

C. Fauquet & D. Fargette (1990) African cassava mosaic virus: etiology, epidemiology, and control. Plant Disease 74, 404411.

J. Felsenstein (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783791.

D.R. Frohlich , I. Torres-Jerez , I.D. Bedford , P.G. Markham & J.K. Brown (1999) A phylogeographical analysis of the Bemisia tabaci species complex based on mitochondrial DNA markers. Molecular Ecology 8, 16831691.

N.J. Gawel & A.C. Bartlett (1993) Characterization of differences between whiteflies using RAPD–PCR. Insect Molecular Biology 2, 3338.

D.M. Hillis & M.T. Dixon (1991) Ribosomal DNA: molecular evolution and phylogenetic inference. Quarterly Review of Biology 66, 411453.

J.P. Legg , R. French , D. Rogans , G. Okao-Okuja & J.K. Brown (2002) A distinct Bemisia tabaci (Gennadius) (Hemiptera: Sternorrhyncha: Aleyrodidae) genotype cluster is associated with the epidemic of severe cassava mosaic virus disease in Uganda. Molecular Ecology 11, 12191229.

J.H. Martin , D. Mifsud & C. Rapisarda (2000) The whiteflies (Hemiptera: Aleyrodidae) of Europe and the Mediterranean Basin. Bulletin of Entomological Research 90, 407448.

M.N. Maruthi , J. Colvin , S. Seal , G. Gibson & J. Cooper (2002) Co-adaptation between cassava mosaic geminiviruses and their local vector populations. Virus Research 86, 7185.

A. Moya , P. Guirao , D. Cifuentes , F. Beitia & J.L. Cenis (2001) Genetic diversity of Iberian populations of Bemisia tabaci (Hemiptera: Aleyrodidae) based on random amplified polymorphic DNA-polymerase chain reaction. Molecular Ecology 10, 891897.

T.M. Perring , A.D. Cooper , R.J. Rodriguez , C.A. Farrar & T.S.J. Bellows (1993) Identification of a whitefly species by genomic and behavioral studies. Science 259, 7477.

C. Schlötterer (1998) Ribosomal DNA probes and primers. In Molecular tools for screening biodiversity. pp 267276. [ A. Karp , P.G. Isaac and D.S. Ingram , editors]. London: Chapman and Hall.

J.D. Thompson , D.G. Higgins & T.J. Gibson (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 46734680.

D. Wool , D. Gerling , B.L. Nolt , L.M. Constantino , A.C. Belloti & F.J. Morales (1989) The use of electrophoresis for identification of adult whitefly in Israel and Colombia. Journal of Applied Entomology 107, 344350.

R.K. Yokomi , K.A. Hoelmer & L.S. Osborne (1990) Relationships between the sweet potato whitefly and the squash silver leaf disorder. Phytopathology 80, 895900.

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Bulletin of Entomological Research
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  • EISSN: 1475-2670
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