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Molecular characterization of whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) populations infesting cassava

  • I. Abdullahi (a1) (a2), S. Winter (a2), G.I. Atiri (a1) and G. Thottappilly (a3)

Bemisia tabaci (Gennadius) populations, collected from cassava and other plants in major cassava-cultivation areas of Sub-saharan Africa and from elsewhere around the world, were studied to determine their biotype status and genetic variation. Random amplified polymorphic DNA–polymerase chain reaction (RAPD–PCR) markers were used to examine the genetic structure of the populations. The dendogram obtained using the neighbour joining method (NJ) split the cassava-associated populations from the non-cassava types with a 100% bootstrap probability. Analysis of molecular variance (AMOVA) of the RAPD fragments revealed that 63.2% of the total variation was attributable to differences among populations, while the differences among groups (host) and within populations accounted for 27.1 and 9.8% respectively. Analysis of the internally transcribed spacer region I (ITS 1) of the ribosomal DNA confirmed that the cassava populations of B. tabaci populations were distinct from non-cassava populations. Experiments to establish whitefly populations on various host plants revealed that cassava-associated populations were restricted to cassava only, whereas B. tabaci from other hosts were polyphagous but did not colonize cassava. Hence, populations of B. tabaci from cassava in Africa represent a distinct group.

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*Fax: +49 (0)531 299 3014 E-mail: S.Winter@BBA.DE
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Abdullahi, I., Atiri, G., Winter, S. & Thottappilly, G. (1998) Differentiation of whitefly (Bemisia tabaci) biotypes from different regions in Africa and their relation to the occurrence of cassava mosaic geminiviruses. In Mitteilungen aus der Biologische Bundesanstalt 357. Braunschweig: 284 (in German).
Banks, G.K., Green, R.L., Cerezo, E.R., Louro, D. & Markham, P.G. (1998) Use of RAPD–PCR to characterize whitefly species in the Iberian Peninsula. p. L 18 in Proceedings of the International Workshop on Bemisia and Geminiviruses.San JuanPuerto RicoJune 7–12 1998.
Banks, G.K., Bedford, I.D., Beitia, F.J., Rodríguez-Cerezo, E. & Markham, P.G. (1999) A novel geminivirus of Ipomoea indica (Convolvulaceae) from southern Spain. In Plant Disease 83, 486.
Bedford, I.D., Briddon, R.W., Markham, P.G., Brown, J.K. & Rosell, R.C. (1992) Bemisia tabaci – biotype characterization and the threat of this whitefly species to agriculture. Proceedings 1992 British Crop Protection Conference – Pests and Diseases 3, 12351240.
Bedford, I.D., Briddon, R.W. & Markham, P.G. (1996) A new species of Bemisia or biotype of Bemisia tabaci, as a future pest of European Agriculture. Proceedings of Plant Health and the European Single Market. British Crop Protection Conference Monograph 54, 381386.
Bellows, T.S., Perring, T.M., Gill, R.J. & Headrick, D.H. (1994) Description of a species of Bemisia (Homoptera, Aleyrodidae). Annals of the Entomological Society of America 87, 195206.
Bharathan, N., Graves, W.R., Narayanan, K.R., Schuster, D.J., Bryan, H.H. & McMillan, R.T. (1990) Association of double stranded RNA with whitefly-mediated silvering in squash. Plant Pathology 39, 530538.
Bird, J. (1957) A whitefly-transmitted mosaic of Jatropha gossypifolia. Agricultural Experimental Station, University of Puerto Rico 22, 135.
Bird, J. & Maramorosch, K. (Eds). (1975) In Tropical diseases of legumes. pp. 171. New York: Academic Press.
Bird, J. & Maramorosch, K. (1978) Viruses and virus diseases associated with whiteflies. Advances in Virus Research 22, 55110.
Brown, J.K., Frohlich, D.R. & Rosell, R.C. (1995) The sweetpotato or silverleaf whiteflies: biotypes of Bemisia tabaci or a species complex?. Annual Review of Entomology 40, 511534.
Brown, J.K., Perring, T.M., Cooper, A.D., Bedford, I.D. & Markham, P.G. (2000) Genetic analysis of Bemisia (Hemiptera: Aleyrodidae) populations by isoelectric focusing electrophoresis. Biochemical Genetics 38, 1325.
Burban, C., Fishpool, D.C., Fauquet, C., Fargette, D. & Thouvenel, J.C. (1992) Host-associated biotypes within West African populations of the whitefly Bemisia tabaci. Journal of Applied Entomology 113, 416423.
Byrne, F.J., Bedford, I.D., Devonshire, A.L. & Markham, P.G. (1995) Esterase variation and squash silverleaf induction in B-type Bemisia tabaci. Bulletin of Entomological Research 85, 175179.
Cahill, M., Johnston, D., Gorman, K. & Denholm, I. (1994) Insecticide resistance in Bemisia tabaci from Pakistan.In Proceedings of the Brighton Crop Protection Conference. pp. 431–436.
Campbell, B.C. (1993) Congruent evolution between whiteflies (Homoptera, Aleyrodidae) and their bacterial endosymbionts based on respective 18S and 16S rDNAs. Current Microbiology 26, 129132.
Carter, S.E., Fresco, L.O., Jones, P.G. & Fairbairn, J.N. (1995) Introduction and diffusion of cassava in Africa. 32 pp. IITA Research Guide no. 49.
Cervera, M.T., Cabezas, J.A., Simon, B., Martinez-Zapater, J.M., Beitia, F. & Cenis, J.L. (2000) Genetic relationships among biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae) based on AFLP analysis. Bulletin of Entomological Research 90, 391396.
Clark, M.A., Baumann, L., Munson, M.A., Baumann, P., Campbell, B.C., Duffus, J.E., Osborne, L.S. & Moran, N.A. (1992) The eubacteria endosymbionts of whiteflies constitute a lineage distinct from the endosymbionts of aphids and mealybugs. Current Microbiology 25, 119123.
Cock, M.J. (1993) Bemisia tabaci an update 1986–. In 1992. pp -. 78 pp. Ascot, Berks: CAB International Institute of Biological Control.
Cohen, S., Duffus, J.E. & Liu, H.Y. (1992) A new Bemisia tabaci biotype in the south western United States and its role in silverleaf of squash and transmission of lettuce infectious yellows virus. Phytopathology 82, 8690.
Costa, A.S. (1976) Whitefly transmitted plant diseases. Annual Review of Phytopathology 14, 429449.
Costa, H.S. & Brown, J.K. (1991) Variation in biological characteristics and esterase patterns among populations of Bemisia tabaci, and the association of one population with silverleaf symptom induction. Entomologia Experimentalis et Applicata 61, 211219.
Costa, A.S. & Russell, L.M. (1975) Failure of Bemisia tabaci to breed on cassava plants in Brazil (Homoptera, Aleyrodidae). Ciencia e Cultura (Sao Paulo) 27. pp 388390.
Costa, H.S., Brown, J.K. & Byrne, D.N. (1991) Host plant selection by the whitefly Bemisia tabaci (Gennadius) (Homoptera, Aleyrodidae) under glasshouse conditions. Journal of Applied Entomology 112, 146152.
Costa, H.S., Johnson, M.W., Ullman, D.E., Omer, A.D. & Tabashnik, B.E. (1993) Sweetpotato whitefly: analysis of biotypes and distribution in Hawaii. Environmental Entomology 22, 1620.
De Barro, P.J. & Driver, F. (1997) Use of RAPD–PCR to distinguish the B biotype from other biotypes of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). Australian Journal of Entomology 36, 149152.
De Barro, P.J., Driver, F., Trueman, J.W.H. & Curran, J. (2000) Phylogenetic relationships of world populations of Bemisia tabaci (Gennadius) using ribosomal ITS 1. Molecular Phylogenetics and Evolution 16, 2936.
Dittrich, V., Hassan, S.O. & Ernst, G.H. (1985) Sudanese cotton and the whitefly: a case study of the emergence of a new primary pest. Crop Protection 4, 161176.
Dubern, J. (1979) Quelques propriétés de la Mosaique Africaine du Manioc. I: La transmission. Phytopathologische Zeitschrift 96, 2539.
Fauquet, C. & Fargette, D. (1990) African cassava mosaic virus: etiology, epidemiology, and control. Plant Disease 74, 404411.
Felsenstein, J. (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783791.
Felsenstein, J. (1994) In Phylogeny Inference Package (PHYLIP), version 3.5c. pp Department of Genetics, University of Washington: Seattle.
Frohlich, D.R., Torres-Jerez, I., Bedford, I.D., Markham, P.G. & Brown, J.K. (1999) A phylogeographical analysis of the Bemisia tabaci species complex based on mitochondrial DNA markers. Molecular Ecology 8, 16831691.
Gawel, N.J. & Bartlett, A.C. (1993) Characterization of differences between whiteflies using RAPD–PCR. Insect Molecular Biology 2, 3338.
Geddes, A.M.W. (1990) The relative importance of crop pests in sub-Saharan Africa. Natural Resources Institute Bulletin 36, 68pp.
Gonzales, R.A., Goldman, G.E., Natwick, E.T., Rosenberg, H.R., Grieshop, J.I., Sutter, S.R., Funakosi, T. & Davila-Garcia, S. (1992) Whitefly invasion in Imperial Valley costs growers workers millions in losses. California Agriculture 46, 78.
Guirao, P., Onillon, J.C., Beitia, F. & Cenis, J.L. (1997) The presence of the 'B' biotype of Bemisia tabaci in France. Phytoma 498, 4448.
Harrison, B.D., Lannon, A.M., Massalski, P.R., Robinson, D.J. & Thomas, J.E. (1986) Geographical variation in African cassava mosaic virus in. Proceedings of the Tenth Plant Virus Epidemiology Workshop. 9–11.
Hillis, D.M. & Dixon, M.T. (1991) Ribosomal DNA: molecular evolution and phylogenetic inference. Quarterly Review of Biology 66, 411453.
Legg, J.P., French, R., Rogans, D., Okao-Okuja, G. & Brown, J.K. (2002) A distinct Bemisia tabaci (Gennadius) (Hemiptera: Sternorrhyncha: Aleyrodidae) genotype cluster is associated with the epidemic of severe cassava mosaic virus disease in Uganda. Molecular Ecology 11, 12191229.
Martin, J.H., Mifsud, D. & Rapisarda, C. (2000) The whiteflies (Hemiptera: Aleyrodidae) of Europe and the Mediterranean Basin. Bulletin of Entomological Research 90, 407448.
Maruthi, M.N., Colvin, J., Seal, S., Gibson, G. & Cooper, J. (2002) Co-adaptation between cassava mosaic geminiviruses and their local vector populations. Virus Research 86, 7185.
Mound, L.A. (1983) Biology and identity of whitefly vectors of plant pathogen. In Plant virus epidemiology. The spread and control of insect-borne viruses. pp 305313. [Plumb, R.T and Thresh, J.M., editors]. Oxford: Blackwell.
Mound, L.A. & Halsey, S.H. (Eds). (1978) In Whiteflies of the World. pp 340 pp. New York: Wiley.
Moya, A., Guirao, P., Cifuentes, D., Beitia, F. & Cenis, J.L. (2001) Genetic diversity of Iberian populations of Bemisia tabaci (Hemiptera: Aleyrodidae) based on random amplified polymorphic DNA-polymerase chain reaction. Molecular Ecology 10, 891897.
Perring, T.M., Cooper, A.D., Rodriguez, R.J., Farrar, C.A. & Bellows, T.S.J. (1993) Identification of a whitefly species by genomic and behavioral studies. Science 259, 7477.
Perry, A.S. (1985) The relative susceptibility to several insecticides of adult whiteflies (Bemisia tabaci) from various cotton growing areas in Israel. Phytoparasitica 13, 7778.
Saitou, N. & Nei, M. (1987) The neighbour-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4, 406425.
Schlötterer, C. (1998) Ribosomal DNA probes and primers. In Molecular tools for screening biodiversity. pp 267276. [Karp, A., Isaac, P.G. and Ingram, D.S., editors]. London: Chapman and Hall.
Schneider, S.Roessli, D. & Excoffier, L. (2000) ARLEQUIN ver. 2.000. A software for population genetics data analysis.
Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 46734680.
White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR protocols. A guide to methods and applications. pp 315322. [Innis, M.A., Gelfand, D.H., Sninsky, J.J. and White, T.J., editors]. New York: Academic Press.
Wool, D., Gerling, D., Nolt, B.L., Constantino, L.M., Belloti, A.C. & Morales, F.J. (1989) The use of electrophoresis for identification of adult whitefly in Israel and Colombia. Journal of Applied Entomology 107, 344350.
Yokomi, R.K., Hoelmer, K.A. & Osborne, L.S. (1990) Relationships between the sweet potato whitefly and the squash silver leaf disorder. Phytopathology 80, 895900.
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