Hostname: page-component-8448b6f56d-42gr6 Total loading time: 0 Render date: 2024-04-24T03:37:55.927Z Has data issue: false hasContentIssue false

A new aphid genus Neoaulacorthum (Hemiptera: Aphididae: Macrosiphini), determined by molecular and morphometric analyses

Published online by Cambridge University Press:  27 August 2010

W. Lee
Affiliation:
Insect Biosystematics Laboratory, Research Institute for Agricultural and Life Sciences, Department of Agricultural Biotechnology, Seoul National University, Seoul 151–921, Korea
H. Kim
Affiliation:
Insect Biosystematics Laboratory, Research Institute for Agricultural and Life Sciences, Department of Agricultural Biotechnology, Seoul National University, Seoul 151–921, Korea
S. Lee*
Affiliation:
Insect Biosystematics Laboratory, Research Institute for Agricultural and Life Sciences, Department of Agricultural Biotechnology, Seoul National University, Seoul 151–921, Korea
*
*Author for correspondence Fax: +82-2-873-2319 E-mail: seung@snu.ac.kr

Abstract

We performed molecular and morphological analyses to determine the generic limit of the genus Aulacorthum, including several species with controversial taxonomic histories. The sequences of four mitochondrial genes, COI, COII, srRNA and lrRNA, and one nuclear gene, EF1a, implied that Aulacorthum is not monophyletic, with Aulacorthum magnoliae and Aulacorthum nipponicum forming a clade that is not sister to other currently recognized Aulacorthum species. Morphometric analysis based on 20 morphological characters also showed that A. magnoliae and A. nipponicum exhibited morphological characteristics distinct from congeneric species. Based on these results, we propose a new genus, Neoaulacorthum ge. n., for A. magnoliae and A. nipponicum.

Type
Research Paper
Copyright
Copyright © Cambridge University Press 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Anstead, J.A., Burd, J.D. & Shufran, K.A. (2002) Mitochondrial DNA sequence divergence among Schizaphis graminum (Hemiptera: Aphididae) clones from cultivated and non-cultivated hosts: haplotype and host associations. Bulletin of Entomological Research 92, 1724.CrossRefGoogle ScholarPubMed
Börner, C. (1952) Europae Centralis Aphides – Die Blättlause Mitteleuropas: Namen, Synonyme, Wirtspflanzen, Generationszyklen. Schriften der Thüringischen Landesarbeitsgemeinschaft für Heilpflanzenkunde und Heilpflanzenbeschaffung in Weimar & Mitteilungen der Thüringischen Botanischen Gesellschaft. Beiheft 3, 1488.Google Scholar
Börner, C. & Heinze, K. (1957) Aphidina-Aphidoidea. Blattläus, plantlice (aphids), pucerons (aphides). pp. 1402 in Blunck, H. (Ed.) Hanbuch der Planzenkrankheiten, 5. Auflage, 5,5,4. Berlin & Hamburg, Germany, Paul Parey.Google Scholar
Blackman, R.L. & Eastop, V.F. (1994) Aphids on the World's Trees: An Identification and Information Guide. 1004 pp. Wallingford, UK, CAB International.CrossRefGoogle Scholar
Blackman, R.L. & Eastop, V.F. (2000) Aphids on the World's Crops: An Identification and Information Guide. 466 pp. 2nd edn. Chichester, UK, John Wiley and Sons Ltd.Google Scholar
Castresana, J. (2002) GBLOCLKS: selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Version 0.91b. Copyrighted by J. Castresana, EMBL.Google Scholar
Choe, H.J., Lee, S.H. & Lee, S. (2006) Morphological and genetic indiscrimination of the grain aphids, Sitobion avenae complex (Hemiptera: Aphididae). Applied Entomology and Zoology 41, 6371.CrossRefGoogle Scholar
Coeur d'acier, A., Jousselin, E., Martin, J.F. & Rasplus, J.Y. (2007) Phylogeny of the genus Aphis Linnaeus, 1758 (Homoptera: Aphididae) inferred from mitochondrial DNA sequences. Molecular Phylogenetics and Evolution 42, 598611.CrossRefGoogle ScholarPubMed
Eastop, V.F. & Blackman, R.L. (2005) Some new synonyms in Aphididae (Hemiptera). Zootaxa 1089, 136.CrossRefGoogle Scholar
Essig, E.O. & Kuwana, S.I. (1918) Some Japanese Aphididae. Proceedings of the California Academy of Sciences 8, 5962.Google Scholar
Farris, J.S., Kallersjo, M., Kluge, A.G. & Bult, C. (1994). Testing significance of incongruence. Cladistics 10, 315319.CrossRefGoogle Scholar
Foottit, R.G., Maw, H.E.L., Pike, K.S. & Miller, R.G. (2010) The identity of Pentalonia nigronervosa Coquerel and P. caladii van der Goot (Hemiptera: Aphididae) based on molecular and morphometric analysis. Zootaxa 2358, 2538.CrossRefGoogle Scholar
Heie, O.E. (1994) The Aphidoidea (Hemiptera) of Fennoscandia and Denmark: Fennoscandia and Denmark: Fauna Entomological Scandinavica V. 242 pp. Scandinavian Science Press, Leiden-Copenhagen.Google Scholar
Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17, 754755.CrossRefGoogle ScholarPubMed
Lee, S. & Kwon, M. (2006) A new aphid of the genus Aulacorthum (Hemiptera: Aphididae) on Magnolia sieboldii K. Koch (Magnoliaeceae) from Korea. The Pan-Pacific Entomologist 82, 332340.Google Scholar
Lee, S.H. (2002) A new genus, Codonopsimyzus, and two species of macrosiphine aphids (Hemiptera: Aphididae) from Korea. The Canadian Entomologist 134, 633646.CrossRefGoogle Scholar
Lee, W., Kim, H., Jan, H. & Lee, S. (2009a) Taxonomic review of the genus Aulacorthum (Sternorrhyncha: Aphididae) for the Korean peninsula, with the description of one new species. Entomological Science 12, 284297.CrossRefGoogle Scholar
Lee, W., Kim, H. & Lee, S. (2009b) Two new species of Aulacorthum (Hemiptera: Aphididae) from Korea. Florida Entomologist 92, 6473.CrossRefGoogle Scholar
Margaritopoulos, J.T., Tzortzi, M., Zarpas, K.D., Tsitsipis, J.A. & Blackman, R.L. (2006) Morphological discrimination of Aphis gossypii (Hemiptera: Aphididae) populations feeding on Compositae. Bulletin of Entomological Research 96, 153165.CrossRefGoogle ScholarPubMed
Margaritopoulos, J., Shigehara, T., Takada, H. & Blackman, R. (2007) Host-related morphological variation within Myzus persicae group (Homoptera: Aphididae) from Japan. Applied Entomology and Zoology 42, 329335.CrossRefGoogle Scholar
Miyazaki, M. (1971) A revision of the tribe Macrosiphini of Japan (Homoptera: Aphididae, Aphidinae). Insecta Matsumurana 34, 1247.Google Scholar
Moran, N.A. (1986) Morphological adaptation to host plants in Uroleucon (Homoptera: Aphididae). Evolution 40, 10441050.CrossRefGoogle ScholarPubMed
Moran, N.A., Kaplan, M.E., Gelsey, M.J., Murphy, T.G. & Scholes, E.A. (1999) Phylogenetics and evolution of the aphid genus Uroleucon based on mitochondrial and nuclear DNA sequences. Systematic Entomology 24, 8593.CrossRefGoogle Scholar
Nylander, J.A., Ronquist, F., Huelsenbeck, J.P. & Nieves-Aldrey, J.L. (2004) Bayesian phylogenetic analysis of combined data. Systematic Biology 53, 4767.CrossRefGoogle ScholarPubMed
Ortiz-Rivas, B., Moya, A. & Martinez-Torres, D. (2004) Molecular systematics of aphids (Homoptera: Aphididae): new insights from the long-wavelength opsin gene. Molecular Phylogenetics and Evolution 30, 2437.CrossRefGoogle ScholarPubMed
Posada, D. & Crandall, K.A. (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics 14, 817818.CrossRefGoogle ScholarPubMed
Remaudière, G. & Remaudière, M. (1997) Catalogue of the world's Aphididae (Homoptera Aphidoidea). Paris INRA.Google Scholar
Shufran, K.A., Burd, J.D., Anstead, J.A. & Lushai, G. (2000) Mitochondrial DNA sequence divergence among greenbug (Homoptera: aphididae) biotypes: evidence for host-adapted races. Insect molecular biology 9, 179184.CrossRefGoogle ScholarPubMed
Swofford, D.L. (2002) PAUP*: Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4.0b10. Sunderland, MA, USA, Sinauer Associates.Google Scholar
Takahashi, R. (1965) Some species of Aulacorthum of Japan (Aphididae, Homoptera). Insecta Matsumurana 27, 99113.Google Scholar
Tamura, K., Dudley, J., Nei, M. & Kumar, S. (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24, 15961599.CrossRefGoogle ScholarPubMed
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 48764882.CrossRefGoogle ScholarPubMed
von Dohlen, C.D., Kurosu, U. & Aoki, S. (2002) Phylogenetics and evolution of the eastern Asian-eastern North American disjunct aphid tribe, Hormaphidini (Hemiptera: Aphididae). Molecular Phylogenetics and Evolution 23, 257267.CrossRefGoogle ScholarPubMed
von Dohlen, C.D., Rowe, C.A. & Heie, O.E. (2006) A test of morphological hypotheses for tribal and subtribal relationships of Aphidinae (Insecta: Hemiptera: Aphididae) using DNA sequences. Molecular Phylogenetics and Evolution 38, 316329.CrossRefGoogle ScholarPubMed
Zhang, D.-X. & Hewitt, G.M. (1996) Nuclear integrations: challenges for mitochondrial DNA markers. Trends in Ecology and Evolution 11, 247251.CrossRefGoogle ScholarPubMed
Supplementary material: File

Lee Supplementary Material

Table .doc

Download Lee Supplementary Material(File)
File 102.9 KB